JPS63158144A - Filtering method - Google Patents

Abstract

PURPOSE:To rapidly and simply perform the solid-liquid separation of a small amount of a specimen, especially, in clinical chemistry, by providing a filter to the lower part of a centrifugal sedimentation tube under tension to place the specimen on said filter and applying the specimen to a centrifugal separator to perform solid-liquid separation. CONSTITUTION:A centrifugal sedimentation tube A is constituted by inserting a filtrate receiving part 2 in a filtering part 1. The filtering part 1 is integrated in such a state that a filter 5 is held between an upper half body 4 and a lower half body 5. Concretely, a flange part 6 is formed in the upper half part 3 and a ring-shaped projection 6a is formed to the under surface of said flange part 6. The lower half body 4 is a cylindrical body having a diameter smaller than that of the upper half body 3 and the upper surface thereof is formed as the support surface 7 of the filter 5. The filtering part 1 is formed by inserting the lower half body 4 in the upper half body 3 in such a state that the filter 5 is placed on the support surface 7, and the filter 5 is held between the support surface 7 and the ring-shaped projection 6a and the abutted part 8 of both of them is welded by high frequency. In filtering, a specimen is placed on the filter 5 and applied to a usual centrifugal separator. A solid and a liquid are separated rapidly and easily.

Description

DETAILED DESCRIPTION OF THE INVENTION <Industrial Application Field> The present invention relates to a filtration method for separating solid and liquid using a centrifuge using a filter.

<Prior art> When performing solid-liquid separation of a sample in which solid matter is dispersed or suspended in a solution, gravity filtration or suction filtration is generally performed using a filter, and the sample is centrifuged or Another method is to separate it into a supernatant layer and a precipitate layer by centrifuging it.

<Problems to be Solved by the Invention> However, the former method has the disadvantage that filtration time is required, and the latter method is extremely difficult to completely remove only the supernatant layer. Furthermore, solid-liquid separation in such a method is not completely performed, and the filtrate and precipitate are quite wet, so that the amount of clear liquid obtained is correspondingly small.

<Means for Solving the Problems> The present inventor investigated a method for quickly and easily separating and filtering solid-liquid, and found that the objective could be achieved by combining filter filtration and centrifugation. The present invention was completed.

That is, the present invention is a filtration method characterized by attaching a filter below a centrifugal sedimentation tube, placing a sample on the filter, and subjecting the sample to a centrifuge for solid-liquid separation.

The present invention will be specifically explained below.

The sample targeted by the present invention has solid matter dispersed in the solution,
Any suspension may be used, but it is especially useful when the amount of sample is 71; when it is desired to obtain a filtrate quickly in a small amount, for example, when removing protein from serum or urine in clinical chemistry. It can be suitably used for.

Further, one embodiment of the centrifugal sedimentation tube used in the present invention will be explained with reference to FIG.
The filter section 1 is welded together with the filter 5 sandwiched between the upper half 3 and the lower half 4.

Specifically, a flange portion 6 is formed inside the upper half body 3 along the inner circumferential surface, and a ring-shaped protrusion 6a for holding down is formed along the inner diameter portion of the lower surface of the flange portion 6.

On the other hand, a support surface 7 for a filter 5 is formed on the upper surface of the lower half 4, which is a cylindrical body with a smaller diameter than the upper half 3. In order to form the filtration section 1, a filter is placed on this support surface 7. In this state, insert the lower half body 4 into the upper half body 3, sandwich the filter 5 between the support surface 7 and the inner ring-shaped protrusion 6a, and insert the lower half body 4 into the upper half body 3.
The abutting portion 8 of the lower half body 4 is welded using high frequency, ultrasonic waves, or the like. The filter used for this purpose is a filter paper or a Chiron filter cloth, and the filter paper can be selected from those commonly used in chemical experiments.

In Figure 1, the filter is held horizontally, but by arranging the filter at an angle, it is possible to move the filter in the direction of the centrifugal vector when performing centrifugation using a swing rotor centrifuge. On the other hand, since the filter is tilted and the solids on the filter paper are accumulated on one side, the filtration rate can be kept high, and the filtration area is increased, so the filtration ability is improved.

In addition, as shown in FIG. 2, the sample reaction tube 10 is structured so as to fit into the upper part of the filtration section 1, and by centrifuging the reaction tube 10 while it is fitted, the sample in the reaction tube can be removed. can all be targeted for filtration.

To perform filtration using such a centrifugal sedimentation tube, a sample is placed on the filter, placed in a conventional centrifuge, and centrifuged. Rotation speed 1000-15000 r, p, m.

After centrifugation for 2 to 20 minutes, the solution is completely transferred to the filtrate receiver.

<Effects of the Invention> As described above, the present invention can perform solid-liquid separation of a sample extremely quickly, and can be particularly effectively used in the field of clinical chemistry, where it is necessary to handle a small amount of sample without waste.

<Examples> Example 1 Serum was filtered using the centrifugal sedimentation tube shown in FIG. still,
The filter was covered with Toyo Roshi NrL5C.

One serum sample was collected in a reaction tube, 0.5 ml of 30% trichloroacetic acid solution was added as a protein removal agent, and the mixture was sufficiently stirred to coagulate and precipitate the proteins.

This reaction tube was covered with a centrifugal sedimentation tube and inverted to transfer the reaction solution onto a filter paper, which was then centrifuged in a swing-type centrifuge at 300 Orp, p, m, for 15 minutes, resulting in a purified protein-free protein. 1.4 ml of serum was obtained.

Example 2 0.51 me of urine from a nephrotic patient was collected in a reaction tube, and 2 ml of 0.33 N perchloric acid was added thereto to precipitate proteins.

Using the same centrifugal sedimentation tube as used in Example 1,
The mixture was centrifuged at 000 r, p, m for 5 minutes to obtain 2.46 ml of clear protein-free solution.

Example 3 A large feces was filtered using a centrifugal sedimentation tube in which the filter 5 shown in FIG. 1 was a nylon filter cloth.

Collect 0.5.7 feces of a dog infected with Fueraria into a reaction tube,
2 ml of physiological saline was added to this and sufficiently suspended.

After covering the tube with a centrifugal sedimentation tube, the tube was inverted, and the tube was then centrifuged in a centrifuge for 5 minutes at 100 Orp, p, m, and filtered.

[Brief explanation of the drawing]

FIG. 1 shows one embodiment of a centrifugal sedimentation tube used in the present invention. Zu bud, = I”i It, 'lWqt slug; □1te'J'+'S. l...filtration section, 2...filtrate receiving section, 5...filter, 10...sample reaction tube

Claims (1)

[Claims] A filtration method characterized by attaching a filter below a centrifugal sedimentation tube, placing a sample on the filter, and subjecting the sample to a centrifuge for solid-liquid separation.