JPS62296873A - Multi-layer flat plate cultivation apparatus - Google Patents
Multi-layer flat plate cultivation apparatusInfo
- Publication number
- JPS62296873A JPS62296873A JP13961886A JP13961886A JPS62296873A JP S62296873 A JPS62296873 A JP S62296873A JP 13961886 A JP13961886 A JP 13961886A JP 13961886 A JP13961886 A JP 13961886A JP S62296873 A JPS62296873 A JP S62296873A
- Authority
- JP
- Japan
- Prior art keywords
- liquid
- plates
- flat plate
- stacked
- attached
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【発明の詳細な説明】
３、発明の詳細な説明
〔産業上の利用分野〕
本発明は、動物細胞等の付着式培養装置に関し、特に培
地交換や、移植、培養回収時の労力を低減できるように
し、かつ同時に付帯設備の必要性を軽減できる多層平板
培養装置に関する。[Detailed Description of the Invention] 3. Detailed Description of the Invention [Field of Industrial Application] The present invention relates to an attached culture device for animal cells, etc., which can particularly reduce labor during culture medium exchange, transplantation, and culture collection. The present invention relates to a multilayer plate culture device that can reduce the need for incidental equipment.
Ｃ従来の技術〕
従来多層平板培養装置は、ルー瓶等の手段に比べ、大量
の培養を扱うのに適した工夫がなされ、その代表的なも
のは特公昭５５−３０８３３である。C. Prior Art] Conventional multilayer plate culture apparatuses have been devised to be more suitable for handling large amounts of culture than methods such as Roux bottles, and a typical example is Japanese Patent Publication No. 55-30833.
従来の多層平板培養装置は、培地注入の際に、人力にて
持ち上げ、横転させる必要があり、培地排出及び培養回
収時には、長時間人力にて持ち上げた状態を維持しなく
てはならなかったゆこのとき、無菌室内で十分な装置と
細心の注意がなければ雑菌混入の恐れがあり、又培養時
には、恒温室内に設置しなければならなかった。Conventional multilayer plate culture devices require manual lifting and overturning when injecting culture media, and must remain lifted manually for long periods of time when discharging the culture medium and recovering the culture. At this time, there is a risk of contamination with various bacteria unless sufficient equipment and care are taken in a sterile room, and the culture must be placed in a thermostatic chamber.
長時間人力にて持ち上げた状態を維持しなければならな
かった問題点を解決するために、横転式架台を用意し、
容易に、その架台に取り付け可能とし、恒温室に入れた
時に場所を小さくするために、容易にその架台から取り
はずせるようにした。In order to solve the problem of having to maintain a lifted state manually for a long time, we prepared a rollover type mount.
The device can be easily attached to the frame, and can be easily removed from the frame in order to reduce the space required when placed in a constant temperature room.
又、Ｉｇ養のために恒温室を設置しなくてはならなかっ
た問題点を解決するために、前記特許請求の範囲第２項
記載の周壁ユニットと、天蓋と底部とを装着可能とした
。Furthermore, in order to solve the problem of having to install a constant temperature room for Ig cultivation, the peripheral wall unit described in claim 2, the canopy, and the bottom can be attached.
第１図は、本発明による多層平板培養装置の複数枚積層
した平板を水平にし、液体を注入したときの断面図であ
り、液体人出栓１に取り付けられたバルブ２を開き、バ
ッキング３によって液もれが防止された？！数枚積層し
た平板中に、斜線で示したように液体を注入すると、注
入された液体の体積と同し体積の空気が、気体人出栓か
ら排出されるので、液体注入時加圧の必要なしに液体が
注入され、第２図は、液体が注入された複数枚積層した
平板に取り付けられたバルブを閉鎖し、横転させた複数
枚積層した平板を示す断面図であり、注入された液体は
、斜線に示す液体は、液体人出孔で連絡し、各平板の底
部に隔てられた状態で下方に移（すＪする。第３図は、
再度水平に手早く戻された複数枚積層しまた平板を示す
断面図であり、斜線部に示す様に液体は、各段の平板に
ほぼ均等に分配され、培養可能状態であることを示して
いる。FIG. 1 is a cross-sectional view of the multilayer plate culture device according to the present invention when a plurality of stacked plates are held horizontally and liquid is injected. Was leakage prevented? ! When liquid is injected into several stacked flat plates as shown by diagonal lines, the same volume of air as the volume of the injected liquid is discharged from the gas tap, so pressurization is not necessary when liquid is injected. Figure 2 is a cross-sectional view showing a plurality of stacked flat plates that have been turned over after closing the valve attached to the plurality of stacked flat plates into which liquid has been injected. The liquid indicated by diagonal lines communicates with the liquid through the liquid outlet hole and moves downward while being separated from the bottom of each flat plate.
This is a cross-sectional view showing a plurality of stacked plates that have been quickly returned to the horizontal plane, and as shown in the shaded area, the liquid is almost evenly distributed to each level of the plates, indicating that they are ready for cultivation. .
第４図は、再度横転させ、液体人出栓が下になる様に傾
斜させ、バルブを開放した。複数枚積層した平板を示す
断面図であり、液体人出栓から排液するに従い、気体人
出栓から空気が流入する為、吸引の必要なしに液体は排
液される。第５図は、横転式架台に取り付けた本発明に
よる多層平板培養装置の外観図であり、図に示すように
、人力にて持ち上げた状態を維持する必要なしに、前記
第１図から第５図で説明した作用を現すことを示す。In Figure 4, the vessel was turned over again, tilted so that the liquid tap was facing down, and the valve was opened. It is a sectional view showing a plurality of laminated flat plates, and as the liquid is drained from the liquid tap, air flows in from the gas tap, so the liquid is drained without the need for suction. FIG. 5 is an external view of the multilayer plate culture apparatus according to the present invention attached to a roll-over type pedestal. This shows that the effect explained in the figure is exhibited.
第６図は、本発明を構成する。周囲が縁高となり、かつ
気体及び液体を供給し得る供給孔を有する平板の斜視図
であり、ａは、横転式架台への取り付け部をもたない平
板の全体図、ｂは横転式架台への取り付け部を有す平板
の部分図であり、第７図は、架台の斜視図であり、基部
１０に立った２本の支柱１１のそれぞれの先端に、ラチ
ェットを有し、支持部１３に取り付けた、本発明による
多層平板培養袋’ＴＩを回転させた場合、各平板が水平
な状態で回転を停止させることができる構造である。FIG. 6 constitutes the invention. It is a perspective view of a flat plate with a peripheral edge height and a supply hole capable of supplying gas and liquid, in which a is an overall view of the flat plate without an attachment part to a rollover type pedestal, and b is an overall view of the flat plate without a part to be attached to a rollover type pedestal. 7 is a partial view of a flat plate having an attachment part, and FIG. When the attached multilayer plate culture bag 'TI according to the present invention is rotated, the structure is such that the rotation can be stopped while each plate is in a horizontal state.
第８図はａが天１７．ｂ、ｃが周壁ユニット、ｄが底部
であり、ａは店開プラスチックを粘り合わせて、中空と
した断熱構造となっており、ｂ、 ｃはアルミ板金の
内壁の外に、発泡プラスチックの断熱材を粘り、ケーシ
ングした構造となっており、特に、Ｃは架台取り付け部
１５を具備し、ｄは二重ガラスの中空板の周囲に’／Ａ
ＢＷＡヒーター１６を具備し、ケーシングした構造と
なっており、複数のｂの中間にＣを、上部にａ、下部に
ｄを積層し、中に本発明による多層平板培養装置を設置
する為に、多層平板培養装置は、任意の温度に設定する
ことができ、恒温室の設備のないところでも使用するこ
とができる。更に第９図に示すように、架台に取り付け
た場合には、人力にて持ち上げることなく容易に培養を
行うことができる。In Figure 8, a is 17. b and c are the peripheral wall units, d is the bottom, a is a hollow insulating structure made of plastic glued together, and b and c are foamed plastic insulation materials outside the inner wall of aluminum sheet metal. In particular, C is equipped with a frame mounting part 15, and d is a hollow plate of double glass.
It is equipped with a BWA heater 16 and has a casing structure, in which C is stacked in the middle of a plurality of b, a is stacked on the top, and d is stacked on the bottom, and in order to install the multilayer plate culture device according to the present invention inside, The multilayer plate culture device can be set at any temperature and can be used even in places without constant temperature room equipment. Furthermore, as shown in FIG. 9, when it is attached to a pedestal, culturing can be easily performed without lifting it manually.
第１図から第４図は、本発明による多層平板培養装置の
作用を示す断面図であり、第５図は本発明による多層平
板培養装置を架台に取り付けた時の作用を示す外観図、
第６図は、本発明による多層平板培養装置を構成する主
要要素である平板の斜視図、第７図は、本発明による多
層平板培養装置を取り付けられる。横転式架台の外観図
、第８図は、本発明による多層平板培養装置に取り付け
られる。ａ天蓋、５周壁ユニツト、Ｃ架台取り付け部を
具備した周壁ユニット、ｄ温調ヒーターを具備する底部
の斜視図、第９図は本発明による多層平板培養装置に天
、１１９と周壁ユニット１８、底部２０及び架台１７を
取り付けたセットの外観斜視図である。
１・・・液体人出栓 ２・・・バルブ３・・・パフキ
ング
４・・・複数枚積層した平板
５・・・気体人出栓 ６・・・液体入出孔７・・・気
体人出孔
８・・・多層平板培′！９装匝
９・・・架台 １０・・・基部１１・・・支柱
１２・・・ラチェット１３・・・支持部
１４・・・温調つまみＩ５・・・架台取り付け部
１６・・・ｍ調ヒーター １７・・・架台１８・・・周
壁ユニット １９・・・天苫２０・・・底部
以上
出願人 セイコー電子工業株式会社
代理人 弁理士 最 上 務（他１名）、６会
第２図
第３図
第６図
１２ °フ〈トコ−・ノド
弔７図
１ｉｉイ木人出十辷1 to 4 are cross-sectional views showing the function of the multilayer plate culture device according to the present invention, and FIG. 5 is an external view showing the function when the multilayer plate culture device according to the present invention is attached to a pedestal,
FIG. 6 is a perspective view of a plate which is a main element constituting the multilayer plate culture apparatus according to the present invention, and FIG. 7 is a perspective view of a plate to which the multilayer plate culture apparatus according to the present invention is attached. FIG. 8 is an external view of the rolling pedestal mounted on the multilayer plate culture apparatus according to the present invention. (a) A canopy, (5) a peripheral wall unit, (C) a peripheral wall unit equipped with a frame attachment part, (d) a perspective view of a bottom part equipped with a temperature control heater, and FIG. 20 and a pedestal 17 are attached. FIG. 1...Liquid outlet valve 2...Valve 3...Puffing 4...Plural laminated flat plate 5...Gas outlet valve 6...Liquid inlet/outlet hole 7...Gas outlet hole 8...Multilayer plate culture'! 9 boxes 9... Frame 10... Base 11... Support 12... Ratchet 13... Support part
14... Temperature control knob I5... Frame attachment part 16... M-adjustment heater 17... Frame 18... Peripheral wall unit 19... Tentom 20... Bottom and above Applicant: Seiko Electronic Industries Co., Ltd. Company agent Patent attorney Tsutomu Mogami (and 1 other person), 6th meeting, Figure 2, Figure 3, Figure 6, Figure 12
Claims (2)
、周囲が縁高となり、かつ気体及び液体を供給し得る供
給孔を有する平板を、複数枚積層し横転式架台に取り付
けられる事を特徴とした多層平板培養装置。(1) Mainly made of a material that allows cells to attach and proliferate, has a high edge around the perimeter, and has supply holes that can supply gas and liquid.It is characterized by being stacked with multiple flat plates and mounted on a rollover type pedestal. A multilayer plate culture device.
断熱材から成る周壁ユニットと、気体／液体入出栓取り
付け孔を具備し、断熱構造となった天蓋と、温調ヒータ
ーを具備した底部とを備える前記特許請求の範囲第１項
記載の多層平板培養装置。(2) A peripheral wall unit that surrounds the surrounding area at the same height as the above-mentioned flat plate and is mainly made of heat insulating material, a canopy that has an insulating structure with gas/liquid inlet/outlet plug mounting holes, and a bottom part that is equipped with a temperature control heater. A multilayer plate culture apparatus according to claim 1, comprising:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13961886A JPS62296873A (en) | 1986-06-16 | 1986-06-16 | Multi-layer flat plate cultivation apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13961886A JPS62296873A (en) | 1986-06-16 | 1986-06-16 | Multi-layer flat plate cultivation apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS62296873A true JPS62296873A (en) | 1987-12-24 |
Family
ID=15249482
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP13961886A Pending JPS62296873A (en) | 1986-06-16 | 1986-06-16 | Multi-layer flat plate cultivation apparatus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62296873A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05501953A (en) * | 1990-09-19 | 1993-04-15 | ベリー，エリック・スコット | Continuous high-density cell culture system |
| DE4406632C1 (en) * | 1994-03-01 | 1995-10-12 | Dietmar Dr Vollbrecht | Solid bioreactor |
| WO2017078007A1 (en) * | 2015-11-06 | 2017-05-11 | 学校法人日本大学 | Culture container |
| JP2020043809A (en) * | 2018-09-18 | 2020-03-26 | テルモ株式会社 | Bioreactors, systems and methods for cell culture |
-
1986
- 1986-06-16 JP JP13961886A patent/JPS62296873A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05501953A (en) * | 1990-09-19 | 1993-04-15 | ベリー，エリック・スコット | Continuous high-density cell culture system |
| DE4406632C1 (en) * | 1994-03-01 | 1995-10-12 | Dietmar Dr Vollbrecht | Solid bioreactor |
| WO2017078007A1 (en) * | 2015-11-06 | 2017-05-11 | 学校法人日本大学 | Culture container |
| JPWO2017078007A1 (en) * | 2015-11-06 | 2018-08-30 | 学校法人日本大学 | Culture vessel |
| US11299699B2 (en) | 2015-11-06 | 2022-04-12 | Nihon University | Culture container |
| JP2020043809A (en) * | 2018-09-18 | 2020-03-26 | テルモ株式会社 | Bioreactors, systems and methods for cell culture |
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