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JPS62240063A - Sterilization and bacteria removal method - Google Patents

Sterilization and bacteria removal method

Info

Publication number
JPS62240063A
JPS62240063A JP61084332A JP8433286A JPS62240063A JP S62240063 A JPS62240063 A JP S62240063A JP 61084332 A JP61084332 A JP 61084332A JP 8433286 A JP8433286 A JP 8433286A JP S62240063 A JPS62240063 A JP S62240063A
Authority
JP
Japan
Prior art keywords
bacteria
present
molded product
fibers
sterilization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP61084332A
Other languages
Japanese (ja)
Other versions
JPH0720845B2 (en
Inventor
睦夫 村上
和雄 寺本
小玉 正智
徹 谷
遠藤 善裕
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toray Industries Inc
Original Assignee
Toray Industries Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toray Industries Inc filed Critical Toray Industries Inc
Priority to JP61084332A priority Critical patent/JPH0720845B2/en
Publication of JPS62240063A publication Critical patent/JPS62240063A/en
Publication of JPH0720845B2 publication Critical patent/JPH0720845B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Apparatus For Disinfection Or Sterilisation (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)

Abstract

(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。
(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

【発明の詳細な説明】 (産業上の利用分野) 本発明は、殺菌及び菌を除去する方法に関する。[Detailed description of the invention] (Industrial application field) TECHNICAL FIELD The present invention relates to a method for sterilizing and removing bacteria.

(従来技術) 我々の生活空間には、各種の細菌、カビ、バクテリア等
の微生物が存在している。そして、?:S温多湿な)2
現下では、それらの繁殖が特に活発であり、繊維の変質
・変色、劣化等の現象を起したり、腐敗・発酵現象をお
こしたり、不快な臭気を発生したりしている。
(Prior Art) Microorganisms such as various bacteria, molds, and bacteria exist in our living spaces. and,? :S warm and humid)2
At present, their proliferation is particularly active, causing phenomena such as deterioration, discoloration, and deterioration of fibers, rotting and fermentation phenomena, and the generation of unpleasant odors.

また、外科手術に際しては近代的な専門技術や極めて複
雑な設備が用いられているにもかかわらず、創傷感染が
相変らず多く、病院での関心の高い事項の1つである。
Furthermore, despite the use of modern specialized techniques and extremely complex equipment during surgical procedures, wound infections remain common and remain a major concern in hospitals.

このため、病原菌による術後感染を防ぎ、傷の治産に役
立てるとか、桑物笠を体内に投与する際、その経路から
の病原微生物侵入を防ぐ材料及σ患名の闘病生活を快い
ものにするなどの医療用繊維製品の開発が望まれていた
For this reason, it can be used to prevent post-operative infections caused by pathogenic bacteria and aid in wound healing, and when administered into the body, it can be used as a material to prevent pathogenic microorganisms from entering the body through that route, and to make the life of a patient fighting illness more pleasant. There was a desire to develop medical textile products such as

従来、抗菌および抗カビ加工法としては、天然または合
成繊維に抗菌力をもつ化合物、たとえば第4級アンモニ
ウム塩などを塗布またはスプレーしたり、化合物溶液に
繊維を含浸する方法が知られている。しかし、これらの
方法では効力に持続性がなく、その後の洗)Rヤ摩擦等
によって容易に抗菌剤が脱落し安全衛生上および排水公
害等の面からも問題でおった。また、抗菌剤を添加した
樹脂を用いて樹脂加工を行なうと繊維の風合を111な
つという欠点をイーしていた。
Conventionally, known antibacterial and antifungal processing methods include coating or spraying natural or synthetic fibers with a compound having antibacterial activity, such as a quaternary ammonium salt, or impregnating the fibers with a solution of the compound. However, these methods do not have long-lasting efficacy, and the antibacterial agent easily falls off due to subsequent washing, rubbing, etc., which poses problems in terms of safety and hygiene, as well as drainage pollution. Furthermore, when resin processing is performed using a resin to which an antibacterial agent is added, the texture of the fibers becomes 111.

(発明が解決しようとづる問題点) 本発明は、空気中、水、水溶液、および血液中に含まれ
る菌を選択的に殺菌おにび菌を除去する方法を捉供づる
一bのである。
(Problems to be Solved by the Invention) The present invention provides a method for selectively sterilizing bacteria and removing bacteria contained in air, water, aqueous solutions, and blood.

(問題点を解決づるだめの手段) 本発明は、次の構成を右する。(Means for solving problems) The present invention has the following configuration.

菌または菌を含有する媒体に、置換すとして下記一般式
(1)の官能基を主鎖または側鎖に右する重合体またほ
ぞの成型品を接触させることを特徴とする殺菌および菌
を除去する方法。
Sterilization and removal of bacteria characterized by contacting bacteria or a medium containing bacteria with a polymer or tenon molded product having a functional group represented by the following general formula (1) in the main chain or side chain as a substitute. how to.

RI    R2 l −CH2−N−C0−C−1・・・・・・・・・(1)
目 上式中、RおJ、びR2は水素1京子または低級アルキ
ル基を承り−0 以下、本発明の詳細な説明する。。
RI R2 l -CH2-N-C0-C-1 (1)
In the above formula, R, J, and R2 each represent hydrogen or a lower alkyl group.The present invention will be described in detail below. .

本発明でいう菌とは、ダラム陰性球菌、グラム陽性好気
性桿菌、ダラム陰性嫌気竹桿菌等のダラム陰性菌や黄色
ブドウ球菌で代表されるグラム陽性菌などを例示するこ
とができる。
Examples of bacteria in the present invention include Durham-negative bacteria such as Durham-negative cocci, Gram-positive aerobic bacilli, and Durham-negative anaerobic bamboo bacilli, and Gram-positive bacteria such as Staphylococcus aureus.

本発明でいう手合体とは、万香族ポリアミド、ポリエス
テル、ポリスルホン、ポリフェニレンサルファイド、ポ
リスチレンなどの手合体を意味するが、中でもポリスチ
レンが化学的に安定であり特に好ましい。また重合体が
精品性ポリプロピレン、・ポリエチレンなどで代表され
るポリα−オレフィンによって補強されていれば、機械
的性質が向上するのでざらに好ましい。
The term "hand coalescence" as used in the present invention refers to hand coalescence of aromatic polyamides, polyesters, polysulfones, polyphenylene sulfides, polystyrenes, etc. Among them, polystyrene is particularly preferred because it is chemically stable. Furthermore, it is more preferable if the polymer is reinforced with a polyα-olefin such as high quality polypropylene or polyethylene, since the mechanical properties will be improved.

上記一般式(1)中、R1およびR2は水素原子または
メヂル基、エヂル基などの低級アルキルL(が挙げられ
るが、中でも水素原子である場合が最も製造しやすい。
In the above general formula (1), R1 and R2 may be a hydrogen atom or a lower alkyl L such as a medyl group or an edyl group, but a hydrogen atom is the easiest to produce.

該重合体中の上記一般式(1)で示される官能基の吊に
は特に限定はないが、少なすぎると該重合体またはその
成型品と菌または菌を含有する媒体との親和性が悪くな
り、処理能力が低下するので、該手合体1gあたり0.
2ミリモル以上、にり好ましくは0.6ミリモル以上存
在するのがよい。
There is no particular limitation on the number of functional groups represented by the above general formula (1) in the polymer, but if there is too little, the affinity between the polymer or its molded product and bacteria or bacteria-containing medium will be poor. Since the processing capacity decreases, the amount of 0.0.
The amount is preferably 2 mmol or more, preferably 0.6 mmol or more.

また、該重合体またはその成型品は使用条件にJ3いて
溶出物がなく、実質上不活性である。すなわち、化学処
理や表面処理で殺菌力を付、すするのとは異なり、その
もの自体が殺菌力を保持しているので安全である。この
ため、長期間の使用でも効果が持続するので産業上の利
用価値は極めて高い。
Furthermore, the polymer or its molded product does not elute under J3 usage conditions and is substantially inactive. In other words, it is safe because it retains its sterilizing power, unlike sipping after adding sterilizing power through chemical treatment or surface treatment. Therefore, the effect remains even after long-term use, so its industrial value is extremely high.

本発明でいう成型品とは繊維、膜、中空糸、粒状物およ
びそれらの高次加工品を意味する。そして、繊維ならば
織物、編物、紙、フェルト、フィルターなどの高次形態
でも用いることができる。
The term "molded product" as used in the present invention means fibers, membranes, hollow fibers, granules, and highly processed products thereof. Fibers can also be used in higher forms such as woven fabrics, knitted fabrics, paper, felt, and filters.

とりわけ、繊維、中空糸が流路を確保できる使用形態に
できるので良い。この特性は成型品を血液のような高粘
性液体の処理剤として使用する簡m要である。この場合
、該成型品の表面積はあまり小さすぎると、菌の処理能
力が低下し、またあまり大きすぎても、本発明成型品を
充填したカラムの通液性は悪くなるので、該成型品の表
面積は0゜01以上50m2/g以下、に リ好マt、
 < ハ、0゜05以上10rn2/g以下がよい。
In particular, fibers and hollow fibers are good because they can be used in a manner that ensures a flow path. This property makes it easy to use the molded product as a treatment agent for highly viscous liquids such as blood. In this case, if the surface area of the molded product is too small, the ability to treat bacteria will be reduced, and if it is too large, the liquid permeability of the column packed with the molded product of the present invention will be poor. The surface area is 0゜01 or more and 50m2/g or less,
<c, preferably 0°05 or more and 10rn2/g or less.

本発明でいう成型品の調製方法の具体例をあげると、多
芯海島型構造でポリプロピレンにより補強したポリエチ
レンpA雑をW+(aとニトロベンゼンの存在下、室温
でパラホルムアルデヒドとN−メヂロールーα−り1コ
ルアセトアミドを用いて不溶化とアミトメデル化をおこ
なったあと、ヨウ化カリウムを含む含水エタノールに浸
し、55℃で4時間加熱することにより連成できる。
To give a specific example of the method for preparing the molded product referred to in the present invention, polyethylene pA miscellaneous with a multicore sea-island structure reinforced with polypropylene is mixed with paraformaldehyde and N-medyrolyl-alpha at room temperature in the presence of W+(a) and nitrobenzene. Coupling can be carried out by insolubilizing and atomedelizing using 1coacetamide, immersing in aqueous ethanol containing potassium iodide, and heating at 55° C. for 4 hours.

本発明でいう成型品を用いて菌または菌を含有する媒体
から殺菌および菌を除去する方法を例示すると、該成型
品を菌または菌を含イjプる媒体に接触させたあと、該
成型品を会頭【すればよい。接触の方法として、該成型
品を充填したカラムを調製し、これに菌または菌を含有
する媒体を通液したり、該成型品をフェルト状または濾
紙状物とし、菌または菌を含有する媒体を濾別する方法
も好ましく用いられる。そして、該成型品はそれ単独で
用いてもよいし、多孔質膜のようなものと組み合せた使
い方も可能である。
To exemplify a method for sterilizing and removing bacteria from bacteria or a medium containing bacteria using the molded article of the present invention, after bringing the molded article into contact with bacteria or a medium containing bacteria, [You should do it]. As a contact method, a column filled with the molded product is prepared and the bacteria or a medium containing the bacteria is passed through the column, or the molded product is made into a felt or filter paper-like material and the molded product is filled with the bacteria or a medium containing the bacteria. Also preferably used is a method of separating by filtration. The molded product may be used alone or in combination with something like a porous membrane.

本発明でいう成型品の使用例をあげると、該成型品を充
填したカラムに輸液、透析液または血液、生理食塩水、
水溶液、水、空気などを循環させる方法、火傷部や偏部
の表面を該成型品で被覆する方法などがある。また、該
成型品から得られる製品を例示すると医療用関連の1i
li維製品としては、血液との接触材お1、エプロン、
ベッドカバー、おむつ、手術用カバー、顔面マスク、女
性の衛生具、失禁用パッド、実験着、洗濯品用のバッグ
、巻包帯、シーツ、枕ケース、ベッドカバー、手術用衣
料、縫合糸などがある。さらに医療用以外では、衣服、
壁紙、じゅうたん、食品の保存容器、食品の包装用具、
掃除機用ゴミ袋などもあげられる。
To give an example of the use of the molded product in the present invention, a column filled with the molded product can be used for infusion, dialysate or blood, physiological saline, etc.
Examples include a method of circulating an aqueous solution, water, air, etc., and a method of covering the surface of a burnt part or uneven part with the molded product. In addition, examples of products obtained from the molded product include medical-related 1i
Li textile products include blood contact materials, aprons,
Bed covers, diapers, surgical covers, face masks, feminine hygiene products, incontinence pads, lab coats, laundry bags, bandages, sheets, pillow cases, bed covers, surgical clothing, sutures, etc. . Furthermore, for non-medical purposes, clothing,
Wallpaper, carpets, food storage containers, food packaging tools,
You can also give garbage bags for vacuum cleaners.

以下に実施例を示す。Examples are shown below.

(実施例) (殺菌および菌除去用材おlの調製) ポリプロピレン(三井゛ノーブレン゛’J3HG)50
部を島成分とし、ポリスチレン(“スタイロン”666
)46部、ポリプロピレン(住友“ノーブレン”WF−
727−F)4部の混合物を海成分とする海島型複合繊
維(層数16、単糸繊度2.6デニール、引張強度2.
9!l]/d 、伸度50%、フィラメント’6142
>50Qを、N−メチロール−α−クロルアセトアミド
50g、ニトロベンゼン400a、98%硫酸400(
]およびパラホルムアルデヒド0.85oからなる混合
溶液中に浸し、20℃で1時間反応させた。繊維を反応
液から取り出し、・0℃の氷水51中に投じて、反応停
止ざゼたのち、水で洗浄し、次に、繊維に付着している
ニトロベンゼンをメタノールで抽出除去した。この繊維
を50℃で真空乾燥して、クロルアセトアミドメチル化
繊維71g (原料繊維)を得た。
(Example) (Preparation of sterilization and bacteria removal material) Polypropylene (Mitsui Noblen'J3HG) 50
part is an island component, and polystyrene (“Styron” 666
) 46 parts, polypropylene (Sumitomo “Noblen” WF-
727-F) sea-island composite fiber containing 4 parts of the mixture as a sea component (number of layers: 16, single yarn fineness: 2.6 denier, tensile strength: 2.
9! l]/d, elongation 50%, filament '6142
>50Q, N-methylol-α-chloroacetamide 50g, nitrobenzene 400a, 98% sulfuric acid 400(
] and 0.85°C of paraformaldehyde, and reacted at 20°C for 1 hour. The fibers were taken out from the reaction solution and poured into ice water 51 at 0°C to stop the reaction, then washed with water, and then the nitrobenzene adhering to the fibers was extracted and removed with methanol. This fiber was vacuum dried at 50° C. to obtain 71 g of chloroacetamide methylated fiber (raw material fiber).

このクロルアセトアミドメチル化、IIfE40oをヨ
1り化カリウム130(1、エタノール800m1おに
び水240m1からなる混合溶液中に浸し、55℃で4
時間加熱してヨード化したあと、エタノールおよび水で
−F分洗浄して本発明例のw4Iftであるヨードアセ
トアミトメデル化繊維(含水度0゜31/PH7,4,
0,32/塩酸型)を得た。
This chloroacetamidomethylated IIfE40o was immersed in a mixed solution consisting of 130 ml of potassium iodide (1), 800 ml of ethanol, 240 ml of rice water, and heated to 55°C for 4 hours.
After iodization by heating for a period of time, the -F portion was washed with ethanol and water to obtain the iodoacetamedomedelized fiber (w4Ift of the present invention) (water content 0°31/PH7,4,
0,32/hydrochloric acid form) was obtained.

(生菌数測定法) 大腸菌(Escherichia  coli  AT
CC25922)を滅菌したリン酸緩衝液に浮遊させ1
06CFu/m1 (集落形成単位)程度のvi度に調
製した。
(Viable bacteria count measurement method) Escherichia coli AT
CC25922) was suspended in a sterilized phosphate buffer solution.
The vi degree was adjusted to approximately 0.6 CFu/m1 (colony forming unit).

繊維と菌液を娠とう後、菌液を3段階希釈(100,1
0,104希釈)し、各0.1mlをDHL寒天培地(
El氷水薬(株)、ニツスイプレート D Hl−寒天
18地〉に接種した。37°C24時間W ’fM 俊
、コロニー数を測定した。
After incubating the fibers and bacterial fluid, dilute the bacterial fluid in three stages (100, 1
0.104 dilution) and 0.1 ml of each was added to DHL agar medium (
Nitsusui Plate D Hl-Agar 18-di> manufactured by El Hyosuiyaku Co., Ltd. was inoculated. The number of colonies was measured at 37°C for 24 hours.

実施例16 実施例で得た本発明例1,2の繊維おにび比較繊維につ
いて以下の殺菌および菌除去実験をおこなった。調製し
た繊lft0.25qを3 cmの長さに切り10m 
lガラス製テストデユープに入れ、8m1の蒸溜水を入
れたあと栓をし、121℃ 30分オートクレーブにか
けた。このあと上澄液を除き、菌液5mlを加えて室温
で娠とうじ、所定84間ごとにサンプリングした。対照
(コントロール)として、菌液のみを注入した試験管を
用意し、同様に娠とうした。所定04間の娠とう後、生
菌数を測定し表1に示す結果を19だ。
Example 16 The following sterilization and bacteria removal experiments were conducted on the comparison fibers of Inventive Examples 1 and 2 obtained in Example. Cut the prepared fiber lft0.25q into 3 cm lengths of 10 m.
The mixture was placed in a glass test duvet, 8 ml of distilled water was added thereto, the stopper was sealed, and the mixture was autoclaved at 121°C for 30 minutes. Thereafter, the supernatant was removed, 5 ml of the bacterial solution was added, and the cells were incubated at room temperature, and samples were taken at predetermined intervals of 84 hours. As a control, a test tube into which only the bacterial solution was injected was prepared and similarly impregnated. After a predetermined period of 04 days of pregnancy, the number of viable bacteria was measured and the result shown in Table 1 was 19.

表1から本発明例では短時間で菌数がゼロになるのに対
し、化学構造のよく似た同じハロゲン原子である比較例
では菌数の低下が見られず効果のないことがわかる。
Table 1 shows that in the inventive example, the number of bacteria becomes zero in a short period of time, whereas in the comparative example, which has a similar chemical structure and uses the same halogen atom, no decrease in the number of bacteria is observed, indicating that there is no effect.

なお、比較例はクロルアセトアミドメチル化繊1! (
含水1哀0.34/PH7,4,0,34/@酸型)を
用いた。
In addition, the comparative example is chloracetamidomethyl synthetic fiber 1! (
Water content 0.34/PH7,4,0,34/@acid type) was used.

実施例2゜ 殺菌効果が繊維からの溶出によるのかどうか調べた。本
発明例の繊維0.250を3 cmの長さに切り10m
1ガラス性テストデユー1に入れ、8rn lの蒸溜水
を入れたあと栓をし、121℃ 30分オートクレーブ
にかりた。このチューブの中から上澄液1mlをとり、
実施例1で述べた大腸菌液5mlを加えて所定n間ごと
に1ナンブリングしながら8時間振とうした。対照(コ
ントロール)として、菌液のみを注入した試験管も同様
に振とうした。振とう後、生菌数を測定し表2に示プ結
宋を19だ。
Example 2 It was investigated whether the bactericidal effect was due to elution from fibers. Cut the fiber 0.250 of the present invention example into a length of 3 cm and a length of 10 m.
1. After adding 8 rnl of distilled water, the mixture was put in a glass Test Dew 1, the stopper was closed, and the mixture was autoclaved at 121°C for 30 minutes. Take 1 ml of supernatant from this tube,
5 ml of the E. coli solution described in Example 1 was added, and the mixture was shaken for 8 hours while numbering once every predetermined interval. As a control, a test tube into which only the bacterial solution was injected was also shaken in the same manner. After shaking, the number of viable bacteria was measured and the result was 19 as shown in Table 2.

表2から本発明例では対照(コントロール)と同レベル
の菌数が確認されたことから、繊維からの溶出物による
殺菌ではないことがわかる。
Table 2 shows that the number of bacteria in the present invention example was the same as that of the control, indicating that the sterilization was not caused by eluates from the fibers.

実施例3゜ 本発明例の繊維を用いてくり返し実験による画処理能力
を調べた。本発明例の繊維0.250を3 cmの長さ
に切り10m1ガラス製テストデユープに入れ、8ml
の蒸溜水を入れたあと栓をし、121℃ 30分オート
クレーブにかけた。このあと上澄液を除き、実施例1で
述べた大腸菌液5m1を加え、1時間振とうした。菌液
をサンプリング後、菌液を捨て新たに同濃度の菌液5m
lを加え、計4回くり返した。サンプリングした試料を
用いて生菌数を測定し表3に示す結果を)qた。
Example 3 Using the fibers of the present invention, the image processing ability was investigated through repeated experiments. Cut the fiber 0.250 of the example of the present invention into a length of 3 cm, place it in a 10 m glass test duplex, and add 8 ml of the fiber.
After adding distilled water, the container was stoppered and autoclaved at 121°C for 30 minutes. Thereafter, the supernatant was removed, 5 ml of the E. coli solution described in Example 1 was added, and the mixture was shaken for 1 hour. After sampling the bacterial solution, discard the bacterial solution and add 5 ml of new bacterial solution with the same concentration.
1 was added and repeated a total of 4 times. The number of viable bacteria was measured using the sampled samples, and the results are shown in Table 3).

表3から本発明例では24 x 10” CFU/ml
l!度の菌液を用いて4回くりし実験を行なっても殺菌
力の低下が少なく効果が維持されていることがわかる。
From Table 3, in the present invention example, 24 x 10” CFU/ml
l! It can be seen that even if the experiment was repeated four times using the same bacterial solution, there was little decrease in bactericidal power and the effect was maintained.

しかも、再生操作をせずに行なうことができた。Moreover, this could be done without any playback operations.

実施例4゜ 本発明例の繊維を用いて菌種を変更して殺菌及び菌除去
実験を行なった。菌種としては、(1)緑膿菌(Psc
udomonas aeruginosa AICC2
7853> (2)霊菌(Serratia marc
csccns ArCC8100) (3)肺炎桿菌(
に1ebsiella pncumoniac ATC
C27736)(4)ネズミチフス菌(SalmOne
lla typhimuriLIm^TCC13311
)を用いた。本発明例の繊#0.25qを3cmの長さ
に切り10m1ガラス製デストチユーブに入れ、8ml
の蒸溜水を入れたあと栓を。
Example 4 Using the fibers of the present invention, sterilization and bacteria removal experiments were conducted with different types of bacteria. As for bacterial species, (1) Pseudomonas aeruginosa (Psc
udomonas aeruginosa AICC2
7853> (2) Serratia marc
csccns ArCC8100) (3) Klebsiella pneumoniae (
1ebsiella pncumoniac ATC
C27736) (4) Salmonella Typhimurium (SalmOne
lla typhimuriLIm^TCC13311
) was used. Cut the fiber #0.25q of the present invention example into 3 cm length, put it in a 10 m1 glass dest tube, and add 8 ml
After adding distilled water, turn on the stopper.

し、121℃ 30分オートクレーブにか()だ。Then autoclave at 121℃ for 30 minutes.

このあと上澄液を除き、菌液5mlを加えて室温で振と
うし、所定時間ごとにサンプリングした。
Thereafter, the supernatant was removed, 5 ml of the bacterial solution was added, the mixture was shaken at room temperature, and samples were taken at predetermined intervals.

対照(コントロール)として、菌液のみを注入した試験
管を用意し、同様に振とうした。所定時間の振とう俊、
生菌数を測定し表4に示す結−果を19だ。
As a control, a test tube into which only the bacterial solution was injected was prepared and shaken in the same manner. Shaking for a specified time,
The number of viable bacteria was measured and the result shown in Table 4 was 19.

表4から本発明例では大腸菌以外のダラム陰性菌に対し
ても強い殺菌効果を有することがわかる。
Table 4 shows that the examples of the present invention have a strong bactericidal effect against Durham-negative bacteria other than E. coli.

実施例5 本発明例の繊維を用いてダラム陽性菌による殺菌および
菌除去実験を行なった。菌種としては、黄色ブドウ球菌
(Staphylococcus aurcus 八T
CC25923)を用いた。本発明例の繊維0.25g
を3Cmの長さに切りlQmlガラス製テストデユープ
に入れ、8mlの蒸溜水を入れたあと栓をし、121℃
 30分オートクレーブにか(プた。この必と」:ff
y液を除き、菌液5mlを加えて室温で娠とうし、所定
時間ごとにサンプリングした。対照(コントロール)と
して、菌液のみを注入した試験管を用意し、同様に娠と
うした。所定時間の振とう後、生菌数を測定し表5に示
す結果を)qた。
Example 5 Sterilization and bacteria removal experiments using Durham-positive bacteria were conducted using the fibers of the present invention. The bacterial species is Staphylococcus aureus (Staphylococcus 8T).
CC25923) was used. 0.25g of fiber of the present invention example
Cut into 3cm lengths and place in a 1Qml glass test duvet, add 8ml of distilled water, stopper, and heat at 121°C.
Autoclave for 30 minutes (this is a must): ff
The y solution was removed, 5 ml of the bacterial solution was added, and gestation was carried out at room temperature, and samples were taken at predetermined intervals. As a control, a test tube into which only the bacterial solution was injected was prepared and similarly impregnated. After shaking for a predetermined period of time, the number of viable bacteria was measured and the results shown in Table 5) were obtained.

なお、培地はトリプトソーヤ寒天培地(日永製薬(株)
、ニツスイプレート)を用いた。
The medium used is trypto soya agar medium (Hinaga Pharmaceutical Co., Ltd.).
, Nitsusui plate) was used.

表5から本発明例ではダラム陽性菌の黄色ブドウ球菌で
も強い殺菌効果を右することがわかる。
Table 5 shows that the present invention exhibits a strong bactericidal effect even on Staphylococcus aureus, a Durham-positive bacterium.

表1 表2 表3 表5 (発明の効果) 本発明は、菌の汚染レベルを低下させ、創傷感染を制t
i!II L/、傷口で無菌状態にすることができる。
Table 1 Table 2 Table 3 Table 5 (Effects of the invention) The present invention reduces the level of bacterial contamination and suppresses wound infection.
i! II L/, the wound can be made aseptic.

しかも、材料からの溶出がないので非毒性、非感作性、
非刺激性であり使用中に抗菌力が低下しないため安全か
つ取扱いやすい。
In addition, there is no elution from the material, so it is non-toxic and non-sensitizing.
It is non-irritating and does not lose its antibacterial activity during use, making it safe and easy to handle.

Claims (1)

【特許請求の範囲】 菌または菌を含有する媒体に、置換基として下記一般式
(1)の官能基を主鎖または側鎖に有する重合体または
その成型品を接触させることを特徴とする殺菌及び菌を
除去する方法。 ▲数式、化学式、表等があります▼・・・・・・(1) 上式中、R_1およびR_2は水素原子または低級アル
キル基を示す。
[Claims] Sterilization characterized by contacting bacteria or a medium containing bacteria with a polymer having a functional group represented by the following general formula (1) as a substituent in its main chain or side chain, or a molded product thereof. and methods for removing bacteria. ▲There are mathematical formulas, chemical formulas, tables, etc.▼・・・・・・(1) In the above formula, R_1 and R_2 represent a hydrogen atom or a lower alkyl group.
JP61084332A 1986-04-14 1986-04-14 How to sterilize and remove germs Expired - Lifetime JPH0720845B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP61084332A JPH0720845B2 (en) 1986-04-14 1986-04-14 How to sterilize and remove germs

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP61084332A JPH0720845B2 (en) 1986-04-14 1986-04-14 How to sterilize and remove germs

Publications (2)

Publication Number Publication Date
JPS62240063A true JPS62240063A (en) 1987-10-20
JPH0720845B2 JPH0720845B2 (en) 1995-03-08

Family

ID=13827554

Family Applications (1)

Application Number Title Priority Date Filing Date
JP61084332A Expired - Lifetime JPH0720845B2 (en) 1986-04-14 1986-04-14 How to sterilize and remove germs

Country Status (1)

Country Link
JP (1) JPH0720845B2 (en)

Also Published As

Publication number Publication date
JPH0720845B2 (en) 1995-03-08

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