JPS62209005A - Textile product for cleaning - Google Patents

Abstract

PURPOSE:To provide the titled product containing protamine, its salt or its derivative as a fungicidal mildew-proofing agent, effective in preventing the generation and proliferation of bacteria and mildew in a cleaning textile product such as mop, kitchen dish-cloth, etc., and having high safety. CONSTITUTION:Protamine, its salt or its derivative is used as an fungicidal mildew-proofing component of the objective product. Generally, protamine is found in the testicle of fish and exists in a testis cell nucleus in the form bonded with deoxyribonucleic acid. Examples of typical protamine are salmine (present in sperm nucleus of salmon), clupeine (present in sperm nucleus of herring), scombrine (present in sperm nucleus of mackerel), etc. Synthetic protamine can be used as well as natural protamine. The treatment of the textile product can be carried out by immersing a textile product in a solution of protamine and drying the product. The amount of protamine attached to the fiber is preferably 0.0001-5wt%, especially 0.001-1wt% based on the fiber.

Description

DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention relates to cleaning lam products, and more specifically to cleaning lam products such as motu, cloth, mat, kitchen dish towel, paper napkin, paper towel, etc. It can prevent the growth and growth of bacteria and mold in cleaning textile products (also called dust control products), and
Cleaning Ia containing a highly safe new antibacterial and antifungal agent
Regarding miscellaneous products.

(Prior Art) Generally, various antibacterial agents and antifungal agents are added to dust control products such as motu, cloths, mats, kitchen napkins, paper towels, and paper towels. Dust control products are used to clean general indoor and outdoor dirt such as dirt and dust, as well as food residue in kitchens and the like, so they also capture bacteria and mold that adhere to them. For this reason, the above-mentioned antibacterial and antifungal agents are used on dust control products to prevent bacteria and mold from growing.

Specifically, in the case of dust control products, 2-4-4'-)dichloro-2'-hydroxydiphenyl ether and paraoxybenzoic acid butyl ester are used as antibacterial agents, and paramethylbenzenesulfomethyldiaiodite is used as a fungicide. etc. are used.

(Problem to be solved by the invention) However, the above-mentioned antibacterial and antifungal agents are not necessarily safe for the human body.In the case of dust control products, each manufacturing company has its own standards. Microbiological tests and animal tests have been conducted by the company, and it has been used only after its safety for humans has been confirmed.

Despite these safety tests, dust control products are often in direct contact with the hands of users, and often by personnel manufacturing the products.
There has been a need for even higher levels of safety.

In other words, in the field of dust control products, there has been a long-awaited antibacterial and antifungal agent that not only has antibacterial activity and antifungal properties, but also has a high level of safety that exceeds general standards.

(Means for Solving the Problems) According to the present invention, protamine, its derivatives, or their salts are used alone or mixed with other substances that increase the antibacterial and antifungal effect for cleaning as an antibacterial and antifungal agent. By incorporating it into textile products, extremely high safety can be obtained while suppressing the growth and proliferation of bacteria and mold.

(Function) In the present invention, protamine is used as an antibacterial and antifungal agent because of its excellent safety.

Generally, protamine is present in the testis of fish, and is present in the nucleus of sperm cells bound to deoxyliponucleic acid. Typical protamines listed in fish include salmin (in the sperm nucleus of salmon), scombrin (in the sperm nucleus of mackerel), cyprinine (in the sperm nucleus of carp), and sturin (in the sperm nucleus of sturgeon). (in the sperm nucleus of bonito) and kazoprotamine (in the nucleus of the sperm of bonito). The fish mentioned above are commonly eaten in the human diet.
In other words, since humans have been eating protamine for a long time in order to survive, the protamine that can be extracted from their testicles is guaranteed to be safe, and it goes without saying that it is non-toxic and harmless. Furthermore, it has been confirmed through long-term human experience that it does not cause any skin disorders.

The present inventors intended to utilize the extremely effective antibacterial and antifungal properties of this highly safe protamine. The antibacterial and antifungal tests of protamines, particularly typical salmin and crupein, will be explained below.

Eight types of standard strains in Table 1, which are often found in dust control products, were used as test bacteria.

Table 1 Standard Plot A loopful of platinum was inoculated into 10 cc of the following heart infusion broth medium and cultured at 36°C for 24 hours, which was used as a test bacterial residue.

Heart Infusion Bouillon Beef heart infusion 500 cc Peptone 10.0 g Sodium chloride 5.0 g Water 1000 cc 0.2 cc of an aqueous solution of salmin or crupein diluted 100 times with distilled water was put into a 1 g paper disk with a diameter of 18+t lio. (manufactured by Toyo Roshi Co., Ltd.) was impregnated and used in the test.

Add 0.5 cc of the above test bacterial solution to 10 cc of heart infusion broth medium and dilute it.
cc is dispensed into Petri dishes in advance.Meanwhile, 20 cc of agar medium prepared by adding 1.5% agar to heart infuge and bouillon is poured into the Petri dish, mixed well, and then solidified. The agar plate containing the bacteria is inverted at 36° C. for 20 to 30 minutes with the lid off to dry the surface of the medium.

The disk is attached to the center of the medium surface of this Petri dish. Thereafter, the Petri dish was inverted and cultured at 36° C. for 24 hours, followed by determination.

The antibacterial properties of the sample were judged based on the presence or absence of an inhibition zone, and the criteria for judgment were as follows.

Table 2 Stopping 1ヱυ■E dealer Hiyotango 1j No blocking belt
− Inhibition zone width is small ± Inhibition zone width is moderate ± Inhibition zone width is large ++ Table 3 shows the results of determining the antibacterial properties of salmin and crupein.

Table 3 As is clear from Table 3, A and B are Durum-negative bacteria.
, C have a slightly weak antibacterial activity, but D, E, F, G, )l, which are Durham-positive bacteria, show extremely strong antibacterial activity without exception, and can be fully used as an antibacterial agent in dust control products. I understand.

Mold Resistance Test As test molds, seven types of standard molds shown in Table 4, which are often found in dust control products, were used.

Table 4 Add 0.0 dioctyl sodium sulfosuccinate to distilled water
Add 0.5% and dissolve it into a test tube and sterilize it in an autoclave.Add standard mold 5 white fungus to this test tube and stir it thoroughly.

The spores are dispersed and used for testing.

Paper discs (manufactured by Toyo Roshi Co., Ltd.) having a diameter of 18 m and a weight of 0.1 g were impregnated with 0.2 cc of an aqueous solution prepared by diluting salmin or crupein 100 times with distilled water and used in the test.

Next, 20 cc of potato dextrose agar medium having the following composition is dispensed into a vetri dish and allowed to solidify.

Potato dextrose agar medium Potato infusion 200.0° Glucose 20. Og agar 15. GK distilled water 1000 cc 0.2 cc of the above spore suspension was dropped onto this agar medium and spread evenly with a conlage rod.

The disk is pasted on the center of the surface of this agar medium, and the judgment is made after incubation at 28° C. for 48 hours. The 0 criterion is the same as the antibacterial test shown in Table 2, and the judgment is made visually.

Table 5 shows the results of determining the antifungal properties of salmin and crupein.
Shown below.

Table 5 As is clear from Table 5, the antifungal power of a is somewhat weak, but b-H's 6! ! Remarkable anti-fungal properties were confirmed against mold. Penicillium, Alpergillus, and Talatosporium make up the majority of the molds, and although some of the molds showed slightly weak results with Alpergillus, the rest showed remarkable mold-proofing power, and protamines such as salmin and crupein control dust. It was found that it can be fully used as a mold preventive agent for products.

The protamine used in the present invention has both antibacterial and antifungal properties, and as a result, it was conventionally used as a mixture of both antibacterial and antifungal agents. By using protamine alone, it is possible to exhibit antibacterial and antifungal properties.

(Description of preferred embodiments of the invention) In the present invention, it is of course possible to use a mixture of a plurality of protamines, or to use a mixture of existing antibacterial agents, antifungal agents and protamine, and to obtain the desired antibacterial activity.・Various modifications can be made to exhibit antifungal properties. Furthermore, as a characteristic of protamine, when an antibacterial and antifungal agent is used together with a surfactant, its effectiveness tends to be reduced in many cases, whereas this phenomenon is not observed with protamine; Can be actively shared with other agents. In other words, it is considered to be optimal as an antibacterial and antifungal agent for cleaning textile products that share surfactants.

Furthermore, recent rapid advances in synthetic chemistry and biotechnology have made it possible to synthesize protamine as a protein, and not only natural protamine but also synthetic protamine, its salts, or its derivatives can be applied to the present invention.

The molecular weight of protamine is approximately 4,000 to 8,000, and as a characteristic of the protein, it has folds in various places.
It is particularly suitable for fibrous dust control products such as paper towels. That is, it is entangled with the fiber bundles of the dust control product and is hard to separate, and can maintain its antibacterial and antifungal effect even after being washed a considerable number of times with normal washing.

Since protamine is soluble in water and does not coagulate due to heat, various methods are used to treat dust control products with protamine as an antibacterial and antifungal agent.

Since protamine is a basic protein, it is easily obtained in the form of salts such as sulfate, hydrochloride, and phosphate, but it can also be used in the form of 'jltm base. Of course, other derivatives having antibacterial and antifungal properties, such as acylated protamine, may also be used.

Protamines (a general term for protamine, its salts, and its derivatives) are used as antibacterial and antifungal agents in cleaning textile products such as cloths, mats, rags, kitchen towels, paper napkins, paper rags, and paper towels. This amount varies depending on the degree of antibacterial properties required and the application, but generally speaking, from 0.0001 to 5% ffi, especially 0.00%, based on the IA fiber.
It is preferable to use it in an amount of 1 to 1% by weight; that is, if this amount is less than the above range, sufficient antibacterial and antifungal properties may not be obtained, and even if it exceeds the above range, There is no particular advantage in terms of the above properties, and it is economically disadvantageous.

In order to contain protamines in H & male bag products for cleaning,
A method of immersing the textile product in a solution of protamines and drying it, or a method of applying a solution of protamines to the UAM product by spray coating or other application means can be adopted.

Cleaning textile products are often coated with or impregnated with dust adsorbents to improve their ability to absorb and retain dust.Oil-based and water-based dust adsorbents are used; The above-mentioned protamines can be blended into the adsorbent and included in cleaning textile products in the form of a protamine-containing dust adsorbent composition. Such a usage mode is particularly desirable, as protamine is easily positively charged. Therefore, it firmly ionically adheres to fibers that tend to be negatively charged, resulting in a sustained action.

When impregnating with an oil agent for dust adsorption such as that normally used in chemical motups, known methods such as adopting an oil-in-water emulsion method in which protamine is dispersed or spraying an oil agent in which protamine is dispersed are used. It can be incorporated at the appropriate stage of the processing method. In this case, it is desirable that the dust adsorbent contains 0.01 to 5% by weight of protamines.

(Operations and Effects of the Invention) According to the present invention, by using protamines present in the testes of fish as an antibacterial and antifungal agent, the occurrence of bacteria and mold in cleaning textile products where they are extremely likely to grow can be prevented. In addition to being able to effectively suppress proliferation, extremely high safety is guaranteed even when the human body comes into direct or indirect contact with cleaning textile products, or when tableware or the like comes into contact with them.

(Example) Examples of cleaning textile products to which an antibacterial and antifungal agent according to the present invention are added will be described in detail below.

100 parts (by weight) of XA tanyu cotton cloth (the same applies hereinafter) to 4 volumes of 20 times
Soak in 0°C warm water and add room temperature non-volatile high boiling point mineral oil 3.
5 parts of zwitterionic activator, 1 part of zwitterionic activator, and 0.5 part of nonionic activator, and further mixed with 0.1 part of salmine and dispersed as an emulsified oil.The liquid becomes cloudy and the temperature is maintained at 40℃. While stirring the cotton cloth, the liquid became clear in about 10 minutes.

That is, mineral oil is adsorbed to the cotton cloth, and salmine is also adsorbed and impregnated.

After dehydration and drying, indoor cleaning was performed once a day for 28 days using this cotton cloth. During this time, no mold growth was observed on the cotton fabric, indicating that the antibacterial and antifungal effects of salmin continued. In addition, after exposing cotton fabric to ethylene oxide gas for one day to kill dust, bacteria, and mold, a test was conducted to determine whether antibacterial and antifungal agents remained within the cotton fabric. A circular piece of cloth with a diameter of 18+++s is cut from cotton cloth, and this circular piece of cloth is used as a disk as described above.
Antibacterial test of species C, D, E, F, G, H,
A mold resistance test was conducted on five types of molds c, d, e j and g. Regardless of the type of bacteria and mold, the size of the inhibition zone was almost the same before and after cleaning, indicating that the antibacterial and antifungal activity continued to work without decreasing. Therefore, salmin was found to be an effective antibacterial and antifungal agent in dust control products.

0.3 parts of Krupein as an antibacterial and antifungal agent was added to 89.7 parts (by weight) of a high-boiling point mineral oil that is nonvolatile at room temperature and stirred to prepare an oil composition, and 20 parts of this oil composition was added. 100 parts of a pile-shaped mop that is rotating and falling is impregnated by spraying using a spray method.

Using this oil-impregnated mop, indoor cleaning was performed once a day for 28 days. No mold growth was observed on this motsup.
It was found that Krupein's antibacterial and antifungal effects were sustained.

In addition, pile pieces were cut from this motsupu and atomized with ethylene oxide gas for one day to eliminate mold and mildew in the dust.
After killing the bacteria, a test was conducted to see if any antibacterial and antifungal activity remained within the pile pieces. Three pile pieces cut to a length of 18 layers were lined up and circumferentially circumferentially with cellophane tape to be used as a sample. Six types of i! iC.

Antibacterial test of D, E, F, G, I (and 5 types of molds c, d).

A mold resistance test was conducted for e, f, and g. It was found that the size of the inhibition zone was almost the same before and after cleaning, regardless of the type of bacteria or mold, indicating that the antibacterial and antifungal effects were sustained. Therefore, Krupein was found to be an effective antibacterial and antifungal agent in dust control products.

XmuA usalmin is dissolved in purified water, and a kitchen towel made of cotton cloth is placed in this aqueous solution. After taking out a kitchen dish towel from the aqueous solution and wringing it out, 0.05 parts by weight of salmine was left in the solution based on 100 parts by weight of the kitchen dish towel.

This kitchen dish towel was used three times a day for nine days, washed every day, dried, and reused. During this time, no mold growth was observed on one kitchen dish towel, indicating that salmin's antibacterial and antifungal properties were maintained.

Every 3 days, cut out a piece of cloth with an 18m square square layer from this dish towel, and use ethylene oxide gas for half a day to kill the mold and bacteria inside the cloth, and then check to see if any antibacterial and antifungal bacteria remain inside the cloth. I conducted some tests. This cloth piece was used in the same manner as in Example 1 to incubate 6 types of bacteria C9D, E, F, G, H.
Antibacterial test of 5 types of molds c and d.

The mold resistance tests e, f, and g were conducted.

The test was conducted a total of four times on day 0, 3 days later, 6 days later, and 9 days later, and the size of the inhibition zone was almost the same as that on day 0 until day 6, indicating that the antibacterial and antifungal activity was It turned out to be persistent.

On the 9th day, the inhibition zone became slightly smaller, and a decrease in antibacterial activity and antifungal activity was observed.

The above results are considered to be due to the following reasons. In general, protamine is positively charged in an aqueous solution, and salmin is no exception.On the other hand, the surface of cotton cloth is negatively charged in water, and the positively charged salmine and negatively charged cotton cloth electrically attract each other. is firmly adsorbed onto the cotton cloth by electric force. In addition, salmine physically entangles with the fibers, reinforcing this bond. As a result, even if the kitchen dish towel was washed again, salmin was difficult to remove from the cotton cloth, and it is thought that its antibacterial and antifungal effects were sufficiently exerted for about 6 days. However, on the 9th day, the desorption due to rewashing became more intense, and the effect seems to have decreased.

Therefore, based on this experiment, if salmin is adsorbed onto the kitchen towel again on the 9th day, the antibacterial and antifungal agent will be restored and the towel can be reused. In particular, in the case of kitchen dish towels, salmin is dissolved in water and just by dipping it into the solution, it can be automatically adsorbed into the fibers by electric force and physical entanglement force, so the adsorption power is extremely high, and it can easily be used at home. can be easily subjected to antibacterial and antifungal treatment. It goes without saying that the same procedure can be applied to other protamines.

Claims (9)

[Claims] (1) A cleaning textile product containing protamine, a salt thereof, or a derivative thereof as an antibacterial and antifungal agent. (2) The cleaning textile product according to claim 1, wherein the protamine is protamine extracted from fish sperm cells. (3) The cleaning textile product according to claim 1, wherein the textile product is a mop. (4) The cleaning textile product according to claim 1, wherein the textile product is cloth. (5) The cleaning textile product according to claim 1, wherein the textile product is matte. (6) The cleaning textile product according to claim 1, wherein the textile product is a cloth. (7) The cleaning textile product according to claim 1, wherein the textile product is a kitchen dish towel. (8) The cleaning textile product according to claim 1, wherein the textile product is a paper product. (9) The cleaning textile product according to claim 1, wherein protamine, its salt, or its derivative is contained in an amount of 0.0001 to 5% by weight per textile product.