JPS62185166A - Apparatus for automatic culture and analysis of liquid specimen - Google Patents

Abstract

PURPOSE:To automatically perform the culture and examination of many kinds of liquid specimens, by arranging a large number of culture containers each comprising a flexible bag in parallel and changing the pressure from the outside. CONSTITUTION:A quantitative pump 14 is operated to suck up a liquid specimen from one of liquid specimen containers 10 and supplies the same to specimen conditioning tanks 28 through a feed pipe 15 and a flow passage change-over apparatus 23. Succeedingly, the apparatus 23 is changed over to operate the quantitative pump 19 of a culture solution tank 17 and an air in injector 16 and a definite amount of a culture solution is injected in the conditioning tanks 28 and both liquids are mixed by stirring devices 27 to prepare liquid specimens with a predetermined diluting ratio while said specimens are introduced into flexible specimen bags 34. Subsequently, the specimen in the other container 10 is diluted with the culture solution by the same operation to prepare separate specimens which are, in turn, injected in other specimen bags 34 to store several kinds or ten and several or more kinds of liquid specimens. Next, the internal pressures of hermetically closed containers 38 are increased and the liquid specimens in the specimen bags 34 are supplied to an examination part 41 by air pressure through liquid supply pipes 35, transfer pipes 32 and a calibrating change-over part 40 and measurement is automatically performed according to a standard dilution method by an electronic operational processing part 43 to which the culture analytical schedule to the liquids to be examined is inputted.

Description

Detailed Description of the Invention (Industrial Application Field) The present invention relates to an automatic culture analyzer, specifically, to efficiently prepare culture samples sequentially from a large number of 1α samples to be tested, culture the sample solution, and The present invention relates to an automatic culture analysis device for liquid samples that can inspect a culture sample every hour, prepare a new culture sample, and record it.

(Prior Art) A BOD measuring device is a commonly known example of a microbial cultivation process investigation test.

This BOD measurement device is implemented based on the semi-dilution method stipulated in 2 Japanese Industrial Standards, Industrial Waste Water K Testing Method JIS-KO+02-21 (i: Ti).

In this method based on the standard dilution method, gases such as air and the culture sample are kept in non-contact during culture to prevent oxygen from being supplied to the culture medium from the outside.

A glass flask (1) as shown in Figure 7 is used as a culture medium, and the amount of decrease in fW oxygen δ degrees (DO) of the culture medium stored in a tightly stoppered state is measured. It uses the amount of oxygen consumed by microbial activity under a certain range of welding conditions suitable for the growth and activity of microorganisms as an index of organic contamination, and is a measurement method that is quite limited in measurement conditions. Also B
OD measurement is extremely delicate and the maximum allowed storage time for the sample used is a short 9 hours, but the results are not known until a 5-day incubation period, and it is one of the measurement methods that cannot be repeated. Therefore, we carefully estimate the results and
A great deal of effort and skilled techniques are required to prepare many types of culture samples by adjusting sample liquids to various dilution rates and to perform accurate measurements. For this reason, automatic devices for the above-mentioned measurement methods have been proposed in recent years. For example, JP-A-55-
The B00 detection device seen in Publication No. 90856 is the 20th
As shown schematically in the figure, a carbon dioxide absorbent (2) is placed at a certain distance in the culture sample, and absorbs and removes the same amount of carbon dioxide gas as the oxygen consumed by the activity of microorganisms.
The BOD value is estimated by knowing the amount of carbon dioxide that has been absorbed and removed using a manometer (3), or by replenishing oxygen corresponding to the amount of carbon dioxide and knowing the amount of replenishment.

Is it also aimed at automatic labor saving? Unexamined Japanese Patent Publication No. 1987- as A place
Publication No. 75062 is mentioned. This automatic 800 measuring device consists of a culture bottle cassette in which culture bottles into which a predetermined amount of pretreated sample water has been injected are arranged and housed in a number corresponding to the number of dilution stages, and a culture bottle in which the cassette is set and the number of culture bottles in each dilution stage is measured. An automatic diluter that dilutes water to a predetermined value, an automatic Do measurement that measures the amount of dissolved oxygen in culture bottles immediately after dilution, and in culture bottles that have been diluted for a culture period, and its measurement. It has a data processing device that calculates the biochemical oxygen demand value of the water sample based on the results.

(Problem to be solved by the invention) Cultivation of microorganisms, etc. is extremely delicate, and if there is a difference in even one of the culture factors, or it is affected by the measurement technology or measurement method, the measured value will fluctuate to some extent. do. Therefore, when comparing cultures of different samples using indicators of culture progress such as PH, turbidity, dissolved oxygen, or rIi element consumption after a certain period of incubation, for example, after 5 days of incubation, it is necessary to compare the cultures of different samples under exactly the same conditions. It is necessary to culture. In addition, based on the standard dilution method, BOD measurement requires the preparation of at least four different test solutions for each test sample, and in the case of a large number of test samples, a test sample with a concentration of four times that of the test sample must be prepared. Requires culture vessel.

However, in conventional devices, glass bottles are used as culture vessels, and it is difficult to prepare a test solution without keeping many of these glass bottles tightly closed, which limits the number of glass bottles that can be arranged.

In the conventional automated BOD measurement device, Atsu-C is applied to at most three to four types of test samples in one device.

(Means for Solving the Problems) The present invention provides a flexible bag with almost no elasticity (this bag was disclosed in Japanese Patent Application No. 60-4) in place of the conventional rigid culture container.
No. 21146) is used as a culture container, and by changing the pressure from the outside of the bag, the volume of the bag is given expansion and contraction behavior, α is made to perform expansion and contraction behavior, and the opening of the bag is A type of pump function is provided to the bag body to introduce liquid into the bag body and to discharge the introduced liquid through a series of liquid guiding pipes.

By installing a large number of such bags in parallel, it is possible to automatically perform airtight culture and testing of test solutions made from multiple samples.
As shown schematically in fig.

(B) Multiple samples) A plurality of suction tubes (1
2) (12), one suction supply pipe (13) extending from the multi-branch cock (11), and a metering pump for sucking up a predetermined amount of sample liquid from one of the sample liquid chambers 7G (10).
14) A sample liquid supply liquid provided with an air injection tube (16) for transporting the sample liquid IJP discharged from the stationary pump (b) through the transport tube (15) using air.

(b) A culture solution tank (17), a washing solution tank (18), a metering pump (19) (20) and a conveying pipe (21) for sending out a predetermined amount of culture solution and washing solution from both tanks (17) and (18).
) (22) A supply unit for a culture solution and a washing solution.

c) A flow path switching device (23) for each of the transport pipes (15) and (21) of the sample liquid supply section and the culture solution supply section.
.

Flow path 1 of the conveying pipe (22) of the cleaning liquid supply section. JJ switching-3A position (24) is provided, and both flow path switching (23) (24) is provided.
) A plurality of I&I pipes (25) and (26) branched from the sample liquid pipe are each equipped with an agitation A device (27) on each discharge port side. Ui department.

(d) Liquid feeding pipes (2) extending from each of the sample adjustment tanks (28)
9), a discharge pipe (30) that communicates with the waste liquid path, a liquid guide pipe (3) that communicates with the culture medium 4 section, a transfer pipe (32) that communicates with the inspection device side, and these four pipes (29) (30). )(31)
(32) A flow path switching device (
33) A sample liquid flow path switching unit comprising (33).

(f) A large number of flexible sample bags (34) (3/l) are arranged in parallel, and a liquid supply pipe (35) is connected to each sample bag (34).
(35) and the liquid guide pipe (31) are connected to the stop valve (3
6) and a culture section that is communicated via a multi-branched cock (37).

(f) Airtight container (38) that covers the outside of the sample bag (34)
) and a suction/exhaust device (39) for adjusting the internal pressure of the closed container (38).

(G) Calibration switching unit (40) and the calibration switching unit (40)
A tester (41) and a test standard solution tank (42) connected to
).

(H) An electronic arithmetic processing unit (43) having a function of reading and processing the detection value of the inspection device (41) and controlling the operation of each movable element of each of the above parts.

It is characterized by having the following.

(Function) When the metering pump (14) of the sample liquid supply section is operated to suck up one of the sample liquid containers (10) from the suction tube (12), the sucked up liquid sample flows into the transport tube (15). sent. Then, when the air injector (16) is operated almost in synchronization with the stoppage of the metering pump (14) to supply air into the conveying pipe (15) for a predetermined period of time, the liquid is drained at the air supply position. At the same time, the liquid sample in the transport pipe (15) is supplied to a predetermined test Ik adjustment tank by this air via the channel cutter 8 device (23) and the liquid sending pipe (25). Next, switch the flow path switch M position (23) and turn on the metering pump (19) and air injector (1) of the culture solution supply section in the same way as above.
When step 6) is operated, a predetermined amount of culture solution is transferred to the sample SI! Injected into the J tank tank 23).

Both solutions are mixed by a stirring device (27) to create a test solution with a predetermined dilution ratio. Once the test solution is created.

First, in order to wash the flexible sample bag (34) during the test tα, a flow path switching device (33) and a stop valve (3) are installed.
6) and the multi-branch cock (37) to connect the liquid supply pipe (29), liquid guide pipe (31), and specific liquid supply pipe (35>), and to The liquid is introduced into the flexible sample bag (34).Then, the flow path cutting device (33
), the suction/exhaust device (39) is operated to supply air into the sealed container (38), and the internal pressure of the sealed container (38) is increased. The liquid is supplied through the liquid supply pipe (35), the liquid guide pipe (31), and the switching device (33).
) and is pushed out towards the discharge pipe (30). By repeating the injection and discharge of the test liquid several times, the test liquid passageway and the sample bag (34) are cleaned with the test liquid.

Thereafter, when a test liquid is injected into the sample bag (34), a pure test liquid without any impurities and in a bubble-free state is stored in the sample bag (34). In this way, several sample bags (34)
When the storage of the same type of test solution is completed within
) to discharge the test liquid remaining in the adjustment tank (28), and then operate the base running pump (20) of the cleaning supply section and the flow path switching position 'A' (24) to perform the above-mentioned test. The adjustment tank (28) into which the liquid has been poured is cleaned with a cleaning liquid.

By the same operation as above, the sample in the pond sample liquid container (10) is supplied to another adjustment tank (28) and diluted with culture solution to create another test liquid. By injecting and storing in the sample bags (34), several types or more than ten test liquids are stored in a large number of sample bags (34).

The test solution thus stored is sent toward the testing section after a preset incubation time has elapsed. That is.

The outside of the sample bag (34) containing the test solution stored for a predetermined incubation period is pressurized by the 1-norm air device (39), and the multi-branch cock (3?), stop valve (36) and flow path switching device ( 33) and select the corresponding sample gt(3).
When the liquid supply pipe (35) of 4) is connected to the transfer pipe (32) via the liquid guide pipe (31), the test liquid in the sample bag (34) flows out toward the transfer pipe (32). 2 calibration switching section (
40) and reaches a tester (41), where the tester (41) performs BOD measurement and the data is recorded and stored in an electronic processing section (43).

(Example) To further explain the drawings showing the embodiments of the present invention in more physical terms, the base supply unit for the liquid sample (10^Xl0B) in the sample liquid container (10) will be explained below. As shown schematically in FIG. 2, the metering pump (14) and the flow path switching device (
23) and the air injection pipe (15) connected to
jl,, to.

We can collaborate on (16). The conveying pipe (15) has an inner diameter of 3 to 5 m.
/ml lx' degree glass tube is applied, air inlet tube (
There is a thin tube (15A) with an inner diameter of 0.3 m/m on the metering pump side where the discharge port of 16A) merges with the conveying pipe (15).
), and after the metering pump (14) is stopped, the conveyor pipe (
15) By injecting 2 air into the tube, the liquid is drained at the outlet of the thin tube (158) as shown in FIGS. 3 and 4, and the conveyance from the outlet of the thin tube (15A) is The liquid sample in the tube (15) is transferred to a predetermined sample adjustment tank (
28).

Such a liquid metering/feeding mechanism is also applied to the transport pipe (21) of the culture solution supply section, and a fixed amount of culture medium (178) sent out by the metering pump (19) is supplied with air in the same manner as above. The liquid is drained by the action of injection 5 (16).

In addition, the liquid being sent out from the liquid cutting surface (15B) in the +if direction does not remain in the transport tube (21) and all of the liquid is sample S11!2! It is arranged to be injected into the tank (28).

Cleaning liquid 1 A cleaning liquid tank (18) storing distilled water, for example.
The washing water (18A) is sent to a transport pipe <22) extending toward an arbitrary sample adjustment tank (28) by a metering pump (20) and a flow path switching device (24), and the transport pipe (22)
A spray nozzle (22A) provided at the discharge port of the cleaning water (188) is sprayed in the form of a shower. The flow path 1; the JJ changing device (24) is connected to the pump (20) (l! A switching valve (46) for selectively communicating the continuous conduit (44^) with the conduit (45), and a switching valve (46) for selectively communicating the conduit (44^) with the conduit (45); It is constituted by a multi-branch cock (47) for communicating with.

Similarly, flow path switching is performed between the transport pipe (15) of the sample liquid supply section, the transport pipe (21) of the culture solution supply section, and the liquid transport pipes (25) extending toward each sample adjustment tank (28). The device (23) is also composed of one switching valve (48) and a multi-branch cock (49) as shown in FIG. (28) can be selectively supplied.

The extending liquid feed pipe (29) is connected to the route cutting device (33). For example, as shown in FIG.
.

The liquid feed pipe (29), the liquid guide pipe (3I) and the transfer pipe (
The adjustment tank (28) is connected to the three-way hole cock (51), and the opening/closing valve (50) and the three-way cock (51) are operated based on a predetermined program. ) Drain the liquid inside or sample a bag (34)
The test solution is introduced into the full sample bag (34), the test solution is discharged from the sample bag (34), and the test solution is transferred to the testing section.

The sample bag (34) is formed by adhesively sealing the periphery of two flexible films on the front and back sides, preferably to a thickness of 15 mm with little elasticity, as shown in FIGS. 8 and 9.
It is formed by adhering (54) two flexible films (52) and (53) of approximately 0 μm with slightly different dimensions in the width direction, and attaching a hollow cap (55) above. A liquid capacity of approximately 100 to 150 cm is applied. And as above, the width dimension is 5"
If a sample bag (34) is formed by two flexible films (52) and (53) of 4, the sample bag (34) can be opened with the hollow cap (55) facing upward as shown in FIG. If the internal liquid is discharged through the hollow cap (55) by applying external pressure after the liquid is injected in this suspended state, the center of gravity of the bag will gradually move downward as the discharge progresses, and the bag will At the top of the front and back films (
52) and (53) are in close contact with each other, thereby making the hollow cap (55
) and the remaining liquid are blocked. That is, as mentioned above, the front and back fills l, (52)
(53) are in close contact with each other, and vertical pleats (56) are formed on the sides of the width-1 wide fill-no, and the pleats (56)
) serves as a path for the remaining fluid to exit again, allowing all of the remaining fluid to be expelled from the body.

FIG. 11 shows a sample bag (34) in which the hollow tube (57) of the hollow cap (55) extends to the bottom of the bag instead of the IJV exit passage forming means using the pleats (56). It is something. In the apparatus of the present invention, 24 or more of the above-mentioned flexible sample bags (34) are arranged in parallel, and each sample bag (
34) The hollow cap (55) is the multi-branched cock (37)
They are each connected to a liquid supply (35) branching from the .

In the embodiment shown in FIG. 1, each of the sample bags (34) is individually housed in a closed container (38). This airtight container (38) has an internal volume of 2 to 2 that of the sample bag (34).
Three times more flexible outer bags are applied, and each of these outer bags is connected to the air conduit (5) of the intake/exhaust device (39) to adjust its internal pressure.
8). The intake and exhaust device (39) does not need to be specified, but as shown in FIG. 1, the intake and exhaust pipe (60) of the air pump (59) is connected to the multi-branch cock (61). It is preferable to connect the air conduits (58) (58) branching from the multi-branch cock (61) to each outer bag constituting the airtight container (38).
Figures 16 to 16 show the sample bag (
34) is an explanatory diagram of the introduction and discharge of liquid into the liquid.

That is, the intake and exhaust device (39) exhausts the air inside the closed container (38) to create a negative pressure inside the closed container (38), and in this state, the stop valve (36) and the switching device (33)
by operating the liquid supply pipe (28) extending from the sample adjustment tank (28).
29) and the liquid supply pipe (35).

The sample bag (
34). The inflow of liquid is stopped when the tension Pf of the sample bag (34) and the pressure P1 of the inflowing liquid are balanced. Next, operate the switching device (33) to connect the transfer pipe (32) and the liquid supply pipe (35), and
'When air is introduced into the closed container (38) by the air device (39) to increase the pressure PI inside the closed container (38),
The pressing force of the pressure P1 acts on the entire outer surface of the sample bag (34), and due to this pressing force, the liquid in the sample bag (3) flows back through the liquid supply pipe (35) to the transfer pipe 32). was leaked to
Finally, as shown in FIG. 15, the front and back sides of the sample bag (34) are brought into close contact, and the internal liquid is almost completely drained.

The sample bag (34) is made of a glass-blocking laminate film when the device of the present invention is used for BOD measurement, and is made of a gas-permeable film when used for tissue culture. It's good to do that.

Further, even if a box-shaped rigid V5 container is used as the airtight container (38), the above-mentioned procedure [injection of the liquid into the sample bag (34) and discharge from the sample bag (34)] can be achieved.

Furthermore, by connecting two adjacent sample bags (34×34) as shown in FIG.

The liquid can be transferred from one bag to the other and can be stirred.

FIG. 18 shows a plurality of double bag bodies (34B) for storing sample bags (-34) inside the outside 'A' (34^), which are further arranged in eight hard sealed containers (38), Exterior (311 people) smoked 1
The pressure can be increased and decreased individually through the JP air pump (50), the multi-branch cock (61) and the air conduit (58), and the pressure inside the closed container (38) can be increased and decreased using another suction/exhaust pump (59A). This shows how this was done. According to this aspect, it is possible to cause several sample bags (34) to simultaneously suck and discharge sample liquid by pressurizing or depressurizing the closed container (38).

Further, as shown in FIG. 19, if a plurality of sample bags (34) (34) without outer bags are housed in a closed container (38) and the inside of the closed container (38) is pressurized or depressurized, Each sample bag (34) (34) can be equipped with the ability to drain or inhale sample liquid.

Desired sample bag (3
It becomes possible to discharge the sample liquid or inject the sample liquid into the sample bag (34) in step 4).

Next, we will explain an example of the use of the device of the present invention. Fig. 1 is an explanatory diagram in which it is applied as an ROD automatic measuring device, and its standard specifications include 24 sample liquid containers (100 liters) in the sample liquid supply section. ), and suction tubes (+2) (12) branched from 240 branch cocks (11) are suspended from each sample liquid container (10) (10). By the operation of the pump (14), a predetermined amount of the liquid to be tested in the sample liquid container (10) is drawn from one suction pipe (12) communicating with the suction supply pipe (13) to a predetermined amount according to the procedure described in 114. Trial adjustment tank (
28) can be injected into any tank. Each sample adjustment tank 28) is connected to a liquid supply pipe (29).
), flow path switching device (33), liquid guide pipe (31>, stop valve (36), 240 branch cocks (37), 24 wooden liquid supply pipes branching from each multi-branch cock (37) (37) (35) Through (35), liquid can be sequentially injected into 96 (4×24) sample bags (34) installed in parallel in the culture section.

In addition, the culture solution (17A) in the culture solution tank (17), the washing solution tank (
18) Cleaning liquid (18^) is also supplied from the electronic processing unit (43) using the metering pump (+9).
(20) is manually prepared and supplied to a predetermined sample adjustment tank (28).

Next, the 800 measurement operation will be described. First, the cleaning liquid supply section is activated to spray a predetermined amount of cleaning liquid (18A) into one of the sample adjustment tanks (28) to clean the sample adjustment tank (28). As the cleaning liquid (18A), distilled water is usually used. Next, the flow path switching device (33) and the stop valve (38
), operate the multi-branch cock (37) to open the sample adjustment tank (
After the washing water in 28) flows into a predetermined sample bag (34), the flow path switching device fif (33) is operated, and external pressure is applied to the sample bag (34) by the action of the suction/exhaust device (39). In addition, the inflowing washing water is made to flow backwards upwards to the 1st outlet pipe (3rd pipe).
0). By repeating the injection and discharging of the cleaning liquid into the sample bag (34) several times in this way, the sample bag (34)
4) is cleaned, and the flow path switching device (23) is cleaned in the same manner.
, (24), (33) and multi-branched cock (37) (3
7), the sample preparation tank (28)
and other sample bags (34) can be cleaned.

Next, adjust the sample solution. First, multi-branched cock (11)
to communicate the suction tube (12) suspended in the desired sample liquid container (10) with the suction supply tube (13),
After sucking up a predetermined amount of sample solution (10^) with a metering pump (14), the air injector (16) is operated to wash a fixed amount of sample solution (IOA) into four sample adjustment tanks (28).
). Next, operate the metering pump (19) and air injector (16) to prepare the pre-programmed culture solution (17A) for four samples in sequence. ! (28) and stirred to prepare a test solution with a dilution ratio nl n2 n3 n4 specified in the standard dilution method specified in 2 Japanese Industrial Standards Industrial Wastewater Test Method JIS 0102, 2+. Afterwards, each sample SI! I tank (28
), the flow path switching device (33), each slip valve (36), and each multi-branch cock (37) of the liquid sending pipe (29) are operated to transfer each of the test liquids with the above four types of dilution 1a ratio into four test liquids. Injecting the test solution into the sample bags (34) (34), preferably draining the injected test solution according to the above-mentioned cleaning procedure, and injecting it again several times. ) (34) to remove impurities other than the test liquid, a predetermined amount of each test liquid was added to each sample gt (
34) It is recommended to inject into (34).

Each test liquid is thus injected into the sample bag (34).

After a preset time of 15 minutes to 120 hours has elapsed, the multi-branch cock (37), stop valve (36), flow path cutter (33), calibration switching unit (40), etc. (43), the predetermined sample bag (34) and the inspection device (41) are brought into communication, and at the same time, the outside of the predetermined sample bag 34 is Pressurized and tested [bag (34)
The test liquid inside is sent toward the test device (41). After sending out the required amount of test liquid, stop valve (36)
is closed, and the dissolved oxygen concentration of the test liquid reaching the test device (41) is measured by the test 2g (41), and the measured value is manually input to the electronic processing unit (43) and recorded. After that, by sequentially performing such operations on the test liquid in each sample bag (34), JISKO102
, 21 can be automatically performed.

As described above, in the apparatus of the present invention, cleaning of each channel and sample bag, injection of sample liquid into the sample bag (34), and discharge of the sample liquid from the sample bag (34) are performed according to a predetermined program. For example, a turbidity meter, a PH meter, a spectrophotometer, a particle counter, an ion chromatograph, an ion electrode, an oxygen electrode, or a liquid chromatograph can be used as the inspection device (41). Also, by using a disinfectant aqueous solution and irises water together as a cleaning solution, can chemicals become resistant to microorganisms? It can also be applied to U-ability tests or plant tissue culture devices.

In addition, the flexible sample bag (34) has a combination of resistance to minute particles + chemical resistance, gas impermeability, or gas permeability depending on the various uses mentioned above. It goes without saying that an appropriate IJ resin film is selected and produced, and the shape of the sample bag is arbitrarily selected depending on the application.

(Effects of the Invention) As detailed above, the automatic 13-mm analyzer for the θ body test ↑'1 (according to the present invention) has a plurality of sample liquid containers (10) suspended from multi-branch cocks (eyes). Suction pipe (I2)
and one suction supply pipe (13) extending from the multi-branch cock (11), and a metering pump (14) for sucking up a predetermined amount of sample liquid from one of the sample liquid 'fI devices (to).
, an air injector (16) that uses air to transport the sample liquid discharged from the metering pump (14) through the transport tube (15).
), a culture solution tank (17), a washing solution tank (18), metering pumps (10) and (20) for delivering a predetermined amount of culture solution and washing solution from both tanks, and a conveyor. A culture solution and washing solution supply section with pipes (2+) (22), and a discharge port of each of the liquid feed pipes (25) and (26) of the sample solution supply section and the culture solution and washing solution supply section. A plurality of sample liquid adjustment tanks (28) equipped with a stirring device (27) arranged therein, a liquid supply pipe (29) extending from each sample liquid adjustment tank (28), and a discharge pipe (29) communicating with a waste liquid path. 3
0), a liquid conduit (31) communicating with the culture section, and an inspection W (
41) and a flow path switching device (33) for connecting any two of these four pipes.
) and a large number of flexible sample bags (
34) are arranged in parallel, and the liquid supply pipe (35) communicating with each sample bag (34) and the liquid guide pipe (31) are connected to each other through a stop valve (36) and a multi-branch cock (37). A pressure adjustment system consisting of a culture section communicating with each other, an airtight container (38) containing the sample gt (34), and an air suction device (39) for increasing and reducing the pressure inside the airtight container (38). section, 2 calibration switching section (40), and the calibration switching section (40).
0) and a test standard solution tank (4
2), and an electronic calculation processing section that has a function of reading and processing the detection values detected by the tester (41) and controlling the movable elements of each of the above parts. , supplying multiple types of test liquids to the sample liquid supply section,
By inputting the culture analysis schedule for these test liquids into the electronic processing unit, for example, as mentioned above, BOD measurements using the standard dilution method of JIS KO102, 21 can be sequentially and automatically carried out, making it possible for experienced chestnut operators to A wide variety of test solutions can be processed efficiently and on schedule without any hassle.

[Brief explanation of drawings]

1 to 19 schematically show embodiments of the present invention, in which FIG. 1 is a configuration diagram of the entire device, FIGS. 2 and 3 are schematic side views of a quantitative supply section for sample liquid, Fig. 4 is a partially enlarged view showing the liquid drain section, Figs. 5, 6, and 7 are explanatory views showing aspects of the flow path switching section, and Figs. 8, 9, and 10 are Front view, sectional view and side view of sample bag, 1st
Figure 1 is a front view showing another aspect of the sample bag, 12th to 16th figures.
The figure is an explanatory diagram of the inflow of liquid into the sample bag due to suction and exhaustion of the airtight container and the ejection of α-isomer. FIG. 19 is an explanatory diagram of other aspects of the pressure adjusting section, and a second
Figure 0 is the conventional BOD it! This is a brief explanation (2) of the Eleventh Basic Law. 1 patent senior Daiwabo Co., Ltd. Figure 2 Figure 3 Figure 8 Figure 12 Figure 14 Figure 16 Figure 17 Figure 19 Figure 18 Figure 20

Claims (1)

[Claims] (a) A plurality of suction pipes hanging from a multi-branch cock to each of a plurality of sample liquid containers, and one suction supply pipe extending from the multi-branch cock, from one sample liquid container. A sample liquid supply unit equipped with a metering pump for sucking up a predetermined amount of sample liquid, and an air injector for transporting the sample liquid discharged from the metering pump through a conveying tube using air. (b) A supply unit for culture solution and washing solution, which includes a culture solution tank, a washing solution tank, and a metering pump and a conveyance pipe for sending out predetermined amounts of culture solution and washing solution from both tanks. (c) A plurality of sample liquid adjustment tanks each including a stirring device disposed at the discharge port of each of the liquid feed pipes of the sample liquid supply section and the culture solution and cleaning solution supply sections. (d) A liquid sending pipe extending from each of the sample liquid adjustment tanks, a discharge pipe communicating with the waste liquid path, a liquid guiding pipe communicating with the culture section, and a transfer pipe leading to the testing device, and any of these four pipes. Sample liquid flow path switching unit equipped with a flow path switching device for connecting two tubes (e) A large number of flexible sample bags are arranged side by side, and a liquid supply pipe and A culture section communicating with the liquid conduit through a stop valve and a multi-branch cock. (F) A pressure adjustment section comprising a flexible exterior and/or a hard sealed container in which the sample bag is housed, and a suction/exhaust device for pressurizing the interior of the exterior and/or the sealed container. (g) An inspection section comprising a calibration switching section, an inspection device communicating with the calibration switching section, and a test standard solution tank. (H) An electronic arithmetic processing unit having a processing function of reading the detection value detected by the above-mentioned inspection device and a function of controlling each movable element of each of the above-mentioned parts. An automatic culture analysis device for liquid samples, characterized by comprising: