JPS62167431A - Gel dyeing apparatus - Google Patents

Abstract

PURPOSE:To make it possible to contact a dyeing solution with a flat plate shaped gel under the same condition by always holding said gel under the same condition, by using a specific main arm and a gel press arm capable of vertically holding the flat plate shaped gel and arranging inflow and outflow pipes to the upper and lower parts of one of the arms. CONSTITUTION:A dyeing apparatus 2 consists of a main frame 21 equipped with two vertical rod shaped parts 21a, 21b and formed by bending both parts into an inverted U shape, a gel press frame 22 supported with respect to said main frame by pivot shafts 27, 28 in a rotatable manner and equipped with two similar vertical rod shaped parts 22a, 22b and formed by bending both parts into an inverted U-shape, the dyeing solution inflow pipe 23 horizontally arranged to the upper part of the gel press frame 22 and the outflow pipe 24 horizontally arranged to the lower part of said frame 22 similarly. A dyeing solution almost uniformly flowing down to the lateral direction from the inflow pipe 23 is sprayed to the flat plate shaped gel G and subsequently absorbed by the outflow pipe 24. By this method, the dyeing solution is supplied to the gel G at a predetermined speed and highly reproducible fixation and dyeing of protein can be performed.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Field of Application) The present invention relates to a gel staining device, and more particularly, to a gel staining device, and more particularly, to a gel staining device, which is capable of staining target polymer components separated on a flat gel by electrophoresis.
For example, the present invention relates to a gel staining device that stains proteins simply and efficiently.

(B) Prior Art Conventionally, operations for staining target macromolecular components such as proteins separated on a flat gel by electrophoresis have been performed one by one manually. That is, the gel after electrophoresis and a predetermined staining solution are placed in a container, shaken for a certain period of time, the staining solution is exchanged several times as necessary, and the next step is decolorization and washing.

(c) Problems to be Solved by the Invention Therefore, the conventional staining operation is extremely time-consuming and manual-filing operation, and is an operation with poor reproducibility.

(d) Means for solving the problem and its operation This invention has two vertical rod-shaped bodies (45) for supporting both sides of a flat gel from one side, and Two vertical rod-like bodies are provided for supporting both sides of the flat gel from the other side and sandwiching the flat gel between the two vertical rod-like bodies of the main arm, Horizontally connecting the upper parts of two vertical rod-like bodies of a gel presser arm pivotally supported to open and the gel presser arm or the main arm,
It is equipped with an inflow pipe that receives the supply of staining solution, and an outflow pipe that horizontally connects the lower parts of the two vertical rod-shaped bodies and allows the staining solution to be discharged. In order to cause the staining solution to flow down the entire surface of the corresponding side channel of the flat gel being held, the inflow pipe has a number of spray ports, and the outflow pipe has a number of absorption ports formed at equal intervals. It is a dyeing device.

That is, this invention uses a specific main arm and a gel holding arm that can vertically hold a flat gel, and furthermore, by arranging an inflow pipe and an outflow pipe for the staining solution above and below in one of these arms. , the plate-shaped gel can always be maintained under the same conditions, and the staining solution can be brought into contact with it under the same conditions.

In this invention, both the main arm and the gel presser arm are equipped with two vertical columnar bodies, and these columnar bodies have smooth surfaces so as not to damage the gel by contact or clamping, for example, Examples of pipes are mentioned as preferred.

(e) Examples The present invention will be described in detail below based on examples shown in the drawings. Note that this invention is not limited to this.

First, in Figures 1 to 3, automatic gel staining system <A)
is an insulated staining tank (1) with a built-in gel staining device (2)
, a dyeing solution switching/recovery section Q71, a dyeing solution switching/introducing section 08), a circulation bomber, a temperature control section (5), and a pipe 09 that connects these in this order for circulation.

The heat insulating tank (1) for staining is made of a heat insulating material, and the plate-shaped gels after electrophoresis are held one by one in the gel staining device (2) inside the heat insulating tank. The staining device (2) has two vertical rod-shaped parts (21a
) (21b), and a main frame (21) formed by bending a pipe into an inverted U-shape, and a pivot shaft (271 (28: not shown) that is rotatable with respect to this main frame. Supported by two vertical rods (22a)
(22b) and a gel presser frame 2 formed by bending the pipe into an inverted U-shape, and a staining solution inflow pipe installed horizontally at the top of this gel presser frame, and a horizontal line at the bottom of the gel presser frame. It consists of an outflow pipe (24) installed at. In addition, the circles are the blowing ports formed at equal intervals at the bottom of the inflow pipe p), (1) are the absorption ports similarly formed in the outflow pipe (241), and the inflow pipe (2) is the temperature control part (5). On the other hand, the outflow pipe 241 goes to the staining solution switching and recovery section (17).
Each is supplied with a staining solution.

Next, the dyeing) 8-liquid switching recovery unit (17) is connected to the pipe (recovery channel c31 via the 1-mill J two-way switching valve (11)) (1-4), and this recovery channel is connected to the switching valve (dying solution tank (additional)...)... through the switching valve (161) and the drain source.The flow path that branches to C via the switching valve (13) is also connected to the drain .

Both trains o'i ot> t and 1 core can be used as appropriate.

The dye solution switching introduction section 08) connects the introduction channel [with] from the pipe aC1l via the switching valve C4), and further connects this introduction channel to the recovery channel c31) with the switching valve 05). It is configured.

The temperature control section (5) is constructed by passing a coiled pipe 09 through the mouth of the constant temperature water tank in order to increase the effects of heat exchange and stirring. The six constant-temperature water tanks are adjusted to a predetermined temperature by freezing the water for one night or the like.

J3, (6) is an absorbance meter that monitors the degree of contamination of the circulating solution, especially during the decolorization operation, and sets the solution exchange signal to 1.
It can be output to the controller (8) described below.

(101 is a dye adsorption column that is bypass-connected from pipe a9 via switching valve 12+. Specifically, it is made of, for example, an activated carbon column, and is switched to J3 during the decolorization and washing process after dyeing.

By the way, (8) is a controller, which controls each switching valve (11).
) (G))......, operation of the circulation pump (7), temperature setting of the humidity control section (5), etc., and automatic control of the dyeing process as well as the decolorization and washing process. .

Here, as shown in Figures 2 and 3, the plate-like gel (G
) is housed in a heat-insulating staining tank (1) while being vertically supported by a gel staining device (2). When the controller (8) turns on the circulation pump, the staining solution in the piping section circulates through the system at a temperature of 20°C (for example, 20°C), and the gel staining device (2 )
The dyeing solution that flows down approximately evenly in the width direction from the inflow pipe (3) is sprayed, flows along the flat plate (G) surface, and is then absorbed into the outflow pipe (2).

In this way, the staining solution controlled at a predetermined temperature is supplied to the surface of the flat gel (G) at a predetermined rate, making it possible to automatically fix and stain proteins with high reproducibility in a short time.

Of course, the controller (
8), first switch the switching valve (1υ) and open the recovery channel Ca.
Collect or discard the dyeing solution d that was being circulated through the dyeing process 11. That is, it is collected into the dyeing solution tank □□□ or discarded from the drain 4 via the switching valve 03). Then, changeover valve 06) O5+ 041 is switched to introduce a new dyeing solution from the dyeing solution tank (humidity is adjusted in advance if necessary).The dyeing solution on the surface is introduced into the piping section through the flow path surface, and for a while, the dyeing solution on the surface remains unchanged. Extrude and collect or dispose of. Then, by switching the switching valve (11), the dyeing solution is circulated again.

After the staining process of the flat gel (G), the staining solution is collected or discarded, and then the decolorization/washing solution is transferred to the tank (G).
It is introduced into the piping section by the same operation as when exchanging the dyeing solution mentioned above, and the circulation pump (7
) is circulated and supplied to the flat gel (G).

(F) Effects of the Invention According to the present invention, the dyeing operation can be performed easily and the reproducibility and efficiency can be improved.

[Brief explanation of drawings]

FIG. 1 is a schematic structural explanatory diagram showing one embodiment of the present invention, FIG. 2 is a side structural explanatory diagram of the gel holder, and FIG. 3 is a front structural explanatory diagram thereof. (1)・・・Insulated tank for dyeing, (2)・・・・・・
Gel staining device, (5)...Temperature control section, 2
1) Main arm, (21a) (21b
)... Vertical rod-shaped part, Stretcher... Gel presser arm, (22a > (22b)... Vertical bar-shaped part, (2J)... Inflow pipe, 24+...
...Outflow pipe, C≦)...Spraying is at the mouth, mountain...Absorption port. Figure 2 Figure 3

Claims (1)

[Claims] 1. A main arm provided with two vertical rod-like bodies for supporting both sides of the flat gel from one side, and a main arm that also supports both sides of the flat gel from the other side. , a gel holding arm that is releasably pivoted to the main arm, and includes two vertical rod-like bodies for sandwiching a flat gel between the two vertical rod-like bodies of the main arm; The upper parts of the two vertical rods of the presser arm or the main arm are connected horizontally, and the inflow tube, which receives the dyeing solution, is connected horizontally to the lower parts of the two vertical rods, and the dyeing solution is supplied. The inflow pipe is equipped with a plurality of spray ports, and the outflow pipe is provided with a plurality of spray ports in order to cause the staining solution to flow down the entire surface of the corresponding side channel of the flat gel held by each of the vertical rods of both arms. A gel staining device consisting of a tube with many absorption ports formed at equal intervals.