JPS6210156B2 - - Google Patents
Info
- Publication number
- JPS6210156B2 JPS6210156B2 JP57191519A JP19151982A JPS6210156B2 JP S6210156 B2 JPS6210156 B2 JP S6210156B2 JP 57191519 A JP57191519 A JP 57191519A JP 19151982 A JP19151982 A JP 19151982A JP S6210156 B2 JPS6210156 B2 JP S6210156B2
- Authority
- JP
- Japan
- Prior art keywords
- electrode
- output
- circuit
- detection
- immobilized enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000001514 detection method Methods 0.000 claims description 23
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 16
- 239000008103 glucose Substances 0.000 claims description 16
- 108010093096 Immobilized Enzymes Proteins 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 9
- 230000002452 interceptive effect Effects 0.000 claims description 8
- 238000010586 diagram Methods 0.000 description 8
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108010015776 Glucose oxidase Proteins 0.000 description 4
- 239000004366 Glucose oxidase Substances 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 229940116332 glucose oxidase Drugs 0.000 description 4
- 235000019420 glucose oxidase Nutrition 0.000 description 4
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 229910052697 platinum Inorganic materials 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 239000013060 biological fluid Substances 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- RGHNJXZEOKUKBD-SQOUGZDYSA-N Gluconic acid Natural products OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005669 field effect Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
【発明の詳細な説明】
（技術分野）
本発明は、固定化酵素電極を用いて生体液中の
成分を測定するグルコース濃度検出装置に関す
る。DETAILED DESCRIPTION OF THE INVENTION (Technical Field) The present invention relates to a glucose concentration detection device that measures components in a biological fluid using an immobilized enzyme electrode.
（背景技術）
近年、酵素電極の名称のもとに生体中の成分を
迅速簡便に測定のできる電極が広く用いられる様
になつてきた。この酵素電極は酵素の固定化技術
の進歩とイオン電極やガス感応電極の発展とが相
まつて出現したものである。その構造は特定の基
質と特異的に反応する酵素膜の部分と系の濃度変
化に応答する従来型の電極部分（下地電極）とか
ら成る二重構造の電極であり、かかる固定化酵素
電極を用いて生体液中の成分を迅速かつ簡便に測
定が可能となつた。(Background Art) In recent years, electrodes that can quickly and easily measure components in living organisms have come into widespread use under the name of enzyme electrodes. This enzyme electrode emerged from the combination of advances in enzyme immobilization technology and developments in ion electrodes and gas-sensitive electrodes. Its structure is a double-structured electrode consisting of an enzyme membrane part that specifically reacts with a specific substrate and a conventional electrode part (substrate electrode) that responds to changes in the concentration of the system. Using this method, components in biological fluids can be measured quickly and easily.
第１図は従来のグルコース濃度検出装置を示す
模式図で、１は血液や尿素の測定溶液、２はグル
コースオキシダーゼ（GOD）を白金電極上に固
定化して成る固定化酵素電極で、試料極を構成す
る。３は白金電極で対極を構成する。４は電流
計、５は定電圧電源である。 Figure 1 is a schematic diagram showing a conventional glucose concentration detection device. 1 is a measurement solution for blood or urea, 2 is an immobilized enzyme electrode consisting of glucose oxidase (GOD) immobilized on a platinum electrode, and the sample electrode is Configure. 3 constitutes a counter electrode with a platinum electrode. 4 is an ammeter, and 5 is a constant voltage power supply.
而して、血液や尿中のグルコース濃度は下記の
反応式によつて測定する。 Therefore, the glucose concentration in blood or urine is measured using the following reaction formula.
即ち、グルコース（β−Ｄ−C6H12O6）は測定
溶液１中に存在する酸素（O2）と共にグルコース
オキシダーゼ（GOD）に特異的に反応してグル
コン酸（C6H12O6）と過酸化水素（H2O2）にな
る。この過酸化水素（H2O2）が下地電極である白
金電極により酸化され負の電子（e-）が生じ、か
かる電子により生ずる電流値を電流計４により測
定することによりグルコース濃度を測定する。 That is, glucose (β-D-C 6 H 12 O 6 ) reacts specifically with glucose oxidase (GOD) together with oxygen (O 2 ) present in the measurement solution 1 to form gluconic acid (C 6 H 12 O 6 ) and hydrogen peroxide (H 2 O 2 ). This hydrogen peroxide (H 2 O 2 ) is oxidized by the platinum electrode serving as the base electrode to generate negative electrons (e - ), and the glucose concentration is measured by measuring the current value generated by these electrons with an ammeter 4. .
しかしながら、血液や尿中にはアスコルビン酸
（C6H8O6）や尿素のように酸化されやすい物質が
存在し、これが下地電極により酸化され下記の如
く反応し、妨害電流を発生させ、正確なグルコー
ス濃度が測定できない事態がおこる欠点があつ
た。 However, there are substances that are easily oxidized such as ascorbic acid (C 6 H 8 O 6 ) and urea in blood and urine, and these are oxidized by the base electrode and react as shown below, generating interference current and making accurate However, there was a drawback that the glucose concentration could not be measured.
C6H8O6→C6H6O6＋2H+2e-
（発明の目的）
本発明は上記欠点に鑑みなされたもので、その
目的とするところは、妨害電流に影響されること
なくグルコースの濃度のみを正確に検出できるグ
ルコース濃度検出装置を提供するにある。 C 6 H 8 O 6 →C 6 H 6 O 6 +2H + 2e - (Objective of the Invention) The present invention has been made in view of the above drawbacks, and its object is to An object of the present invention is to provide a glucose concentration detection device that can accurately detect only the concentration of glucose.
（発明の開示）
第２図は本発明に係るグルコース濃度検出装置
の原理図で、固定化酵素電極２と対極３とにより
構成される従来と同様の検出回路Ａに、固定化酵
素電極２の下地電極と同一種の材質より構成され
る電極６と対極３より構成される妨害物質濃度検
出回路Ｂを加え、両検出回路Ａ，Ｂの出力を相殺
回路Ｃに入力することにより固定化酵素に特異的
に反応するグルコースの濃度のみを検出するよう
にしたものである。尚、第３図ａ〜ｃは上記各検
出回路Ａ，Ｂ，Ｃの出力レベルを示す特性図で、
同図ａは検出回路Ａの出力を示し、グルコース濃
度と妨害物質濃度が足された出力レベルを示す。
同図ｂは検出回路Ｂの出力を示し、妨害物質濃度
を示す。同図ｃは相殺回路Ｃの出力を示し、グル
コース濃度のみを示す。(Disclosure of the Invention) FIG. 2 is a principle diagram of the glucose concentration detection device according to the present invention. By adding an interfering substance concentration detection circuit B consisting of an electrode 6 made of the same material as the base electrode and a counter electrode 3, and inputting the outputs of both detection circuits A and B to a cancellation circuit C, the immobilized enzyme can be detected. It is designed to detect only the concentration of glucose that specifically reacts. Incidentally, FIGS. 3 a to 3 c are characteristic diagrams showing the output levels of each of the above-mentioned detection circuits A, B, and C.
Figure a shows the output of the detection circuit A, and shows the output level that is the sum of the glucose concentration and the interfering substance concentration.
Figure b shows the output of detection circuit B, indicating the interfering substance concentration. Figure c shows the output of the cancellation circuit C, and shows only the glucose concentration.
第４図は本発明の一実施例を示す回路図であ
る。図中５は定電圧回路で、オペアンプOP１の
正端子に定電圧を与えてオペアンプOP１の出力
電圧を一定とするため、抵抗R1、電界効果型ト
ランジスタFET、ツエナーダイオードZDから成
る直列回路と、オペアンプOP１の出力電圧を決
める。抵抗R2，R3と、出力電圧を更に分圧する
抵抗R4，R5とより成る。そして、この定電圧回
路５の出力を対極３に印加している。７は前記検
出回路Ａで、オペアンプOP２と抵抗R6とからな
り抵抗R6に流れる電流の大きさに応じた出力電
圧を出力端より出力する。８は前記検出回路Ｂ
で、オペアンプOP３と抵抗R7とからなり抵抗R7
に流れる電流の大きさに応じた出力電圧を出力端
より出力する。９は前記相殺回路で、オペアンプ
OP４と抵抗R8，R9，R10，R11とからなり、前記
両検出回路７，８の出力電圧の差を出力端より出
力する。 FIG. 4 is a circuit diagram showing one embodiment of the present invention. 5 in the figure is a constant voltage circuit, in order to apply a constant voltage to the positive terminal of the operational amplifier OP1 and keep the output voltage of the operational amplifier OP1 constant, a series circuit consisting of a resistor R 1 , a field effect transistor FET, and a Zener diode ZD; Determine the output voltage of operational amplifier OP1. It consists of resistors R 2 and R 3 and resistors R 4 and R 5 that further divide the output voltage. The output of this constant voltage circuit 5 is applied to the counter electrode 3. 7 is the detection circuit A, which is composed of an operational amplifier OP2 and a resistor R6 , and outputs an output voltage from an output terminal according to the magnitude of the current flowing through the resistor R6 . 8 is the detection circuit B
The resistor R7 consists of the operational amplifier OP3 and the resistor R7 .
The output terminal outputs an output voltage according to the magnitude of the current flowing through the output terminal. 9 is the above-mentioned cancellation circuit, which is an operational amplifier.
It consists of OP4 and resistors R 8 , R 9 , R 10 , and R 11 , and outputs the difference between the output voltages of both the detection circuits 7 and 8 from the output terminal.
（発明の効果）
本発明は上記のように、試料極としてグルコー
ス濃度を測定する固定化酵素電極を用い、前記固
定化酵素電極を構成する下地電極と同一種類の妨
害物質濃度検出電極を配し、前記固定化酵素電極
と妨害物質濃度検出電極の各出力をそれぞれの検
出回路に入力し、その両検出回路の出力を相殺回
路に入力して両検出回路出力の差を出力し、その
出力により濃度を測定したので、妨害物質による
影響のない固定化酵素に反応するグルコース濃度
のみを正確に検出することができるグルコース濃
度検出装置を提供できた。(Effects of the Invention) As described above, the present invention uses an immobilized enzyme electrode for measuring glucose concentration as a sample electrode, and arranges an interfering substance concentration detection electrode of the same type as the base electrode constituting the immobilized enzyme electrode. , input the respective outputs of the immobilized enzyme electrode and the interfering substance concentration detection electrode to their respective detection circuits, input the outputs of both detection circuits to a canceling circuit, output the difference between the outputs of both detection circuits, and use the outputs to Since the concentration was measured, it was possible to provide a glucose concentration detection device that can accurately detect only the glucose concentration that reacts with the immobilized enzyme without being affected by interfering substances.
第１図は従来例の模式図、第２図は本発明に係
る原理図、第３図ａ〜ｃは各検出回路の出力特性
図、第４図は本発明の一実施例を示す回路図であ
る。
Fig. 1 is a schematic diagram of a conventional example, Fig. 2 is a principle diagram according to the present invention, Figs. 3 a to c are output characteristic diagrams of each detection circuit, and Fig. 4 is a circuit diagram showing an embodiment of the present invention. It is.
Claims (1)
と、前記固定化酵素電極を構成する下地電極と同
一種類の妨害物質濃度検出電極とを配し、前記固
定化酵素電極と妨害物質濃度検出電極の各出力を
それぞれの検出回路に入力し、その両検出回路の
出力差を相殺回路を介して検出して成るグルコー
ス濃度検出装置。1. An immobilized enzyme electrode for measuring glucose concentration and an interfering substance concentration detection electrode of the same type as the base electrode constituting the immobilized enzyme electrode are arranged, and each output of the immobilized enzyme electrode and interfering substance concentration detection electrode is arranged. is input into each detection circuit, and the difference in output between the two detection circuits is detected via a canceling circuit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57191519A JPS5982082A (en) | 1982-10-29 | 1982-10-29 | Apparatus for determining glucose concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57191519A JPS5982082A (en) | 1982-10-29 | 1982-10-29 | Apparatus for determining glucose concentration |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5982082A JPS5982082A (en) | 1984-05-11 |
| JPS6210156B2 true JPS6210156B2 (en) | 1987-03-04 |
Family
ID=16276001
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57191519A Granted JPS5982082A (en) | 1982-10-29 | 1982-10-29 | Apparatus for determining glucose concentration |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5982082A (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8508677D0 (en) * | 1985-04-03 | 1985-05-09 | Genetics Int Inc | Assay for salicylate |
| JPS6222552U (en) * | 1985-07-25 | 1987-02-10 | ||
| JP2678348B2 (en) * | 1994-11-07 | 1997-11-17 | 全国学校用品株式会社 | Writing brush case |
| PT1077634E (en) | 1998-05-13 | 2003-12-31 | Cygnus Therapeutic Systems | MONITORING OF PHYSIOLOGICAL SUBSTANCES TO BE ANALYZED |
-
1982
- 1982-10-29 JP JP57191519A patent/JPS5982082A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5982082A (en) | 1984-05-11 |
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