JPS6125314B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a separated sample extrusion device for collecting lymphocytes from a sample after centrifugation, for example.

When collecting lymphocytes from blood, a blood collecting syringe 1 is directly centrifuged as shown in FIG. Figure 2 shows the state after centrifugation.
When collecting only lymphocytes 6 from a separated sample, conventionally, the lymphocytes 6 were collected by manually pressing a syringe while visually observing the separated layer of lymphocytes 6. In the conventional collection method described above, collection is performed by pushing the syringe 1 manually, so it is not easy to push out only the lymphocytes 6.
Moreover, it is impossible to efficiently collect only lymphocytes 6. In FIGS. 1 and 2, 2 is blood, 3 is a microtube, 4 is a specific gravity liquid, and 5 is a piston.

An object of the present invention is to easily control the amount of extrusion and efficiently collect lymphocytes, unlike the conventional method of manually pressing a separated layer of lymphocytes while visually observing the separation layer.

The present invention will be explained below with reference to the embodiments shown in FIGS. 3 to 5.

In FIGS. 3 and 4, 11 is a case. 12 is a slide holder fixed inside the case, and side plates 13, 13 have vertically long guide holes 1.
4 and 14 have been established. Sliders 13 and 15 are disposed inside the side plate 13 and close to the side plate 13, and the sliders 15 and 15 are connected to the guide holes 14 and 14.
Screw 1 passed through and fixed to slider 15, 15
6 and 16 allow vertical movement relative to the slide holder 12. A horizontally arranged screw shaft 17 is rotatably supported on the slider 15. The screw shaft 17 has a right-hand threaded part 18 and a left-hand threaded part 19 with its approximate center as a boundary, and one end 21 protrudes from the case 11 and has a knob 20 fixed thereto. Although not shown, the part of the case 11 from which the one end 21 of the screw shaft 17 protrudes has a vertically long elongated hole, and the one end 21 protrudes from the elongated hole. The screw shaft 17 has slide screws 22 on each of the right-hand threaded portion 18 and the left-hand threaded portion 19.
23 are screwed together. 24 and 25 are a pair of arms, the upper ends of which are rotatably connected to a fulcrum 33 fixed to the slide holder 12 with a pin or the like;
Its lower ends are rotatably connected to slide screws 22 and 23, respectively. Furthermore, arms 24, 25
The upper end of may be connected to the case 11. A slide base 26 is horizontally fixed to the lower end of the slider 15, and the slide base 26 has a plurality of syringe pressing parts 27, 27, . . . that protrude downward. The syringe pressing parts 27, 27, . . . are constructed with screws screwed into the slide base 26 so that the amount of protrusion of each can be adjusted.

With the above configuration, when the screw shaft 17 is rotated counterclockwise by the knob 20, the slide screws 22, 23
will move in the direction of arrow A. As a result, the arms 24, 25 are rotated in the direction of arrow B, and the slide screws 22, 23, screw shaft 17, slider 15, and slide base 26 are each moved downward. Conversely, it is clear that if the knob 20 is rotated clockwise, it will be moved upward. A syringe holder 28 fixed to the case 11 is arranged horizontally below the slide base 26, so that the syringe 1 can be arranged directly below the syringe pressing parts 27, 27, . . . . The syringe holder 28 has a substantially U-shaped side surface, and its upper plate 2
9 and the lower plate 30 each have a plurality of U-shaped grooves for inserting and holding the syringe 1 therein. 31 is a test tube holder placed on the upper surface of the bottom plate of the case 11, and 32 is a test tube.

To collect lymphocytes, they are held in a syringe holder 28 with a syringe shown in FIG. 2 after centrifugation. Then, rotate the knob 20 counterclockwise to push down the slide base 26 and press the piston 5 with the syringe pressing part 27 to first push out the specific gravity liquid 4 into a test tube etc. Next, set up the test tube 32 for collecting lymphocytes and Turn knob 20 while visually checking the separation layer in step 6.
is rotated counterclockwise to push out the separated layer of lymphocytes 6 and collect them into a test tube 32.

FIG. 5 shows the relationship between the moving positions of the slide screws 22 and 23 and the corresponding downward speed of the slide base 26 when the rotation of the screw shaft 17 is kept constant. . As shown in Figure 5, D
In the range, the descending speed of the slide base 26 is fast,
It can be seen that the descending speed is slow in the C range. Therefore, it can be understood that the D range is suitable for pushing out the specific gravity liquid 4, and the C range is suitable for pushing out the separated layer of lymphocytes 6 because of slow velocity control.

According to the present invention, since the amount of extrusion of the separated sample can be easily controlled, the desired separated layer can be efficiently fractionated.

[Brief explanation of the drawing]

FIG. 1 is a front view showing the state of the sample before centrifugation, and FIG. 2 is a front view showing the state of the sample after centrifugation. Figure 3 is a front view of the device of the present invention;
The figure is a side view seen from direction A in FIG. 3. FIG. 5 shows the moving position of the slide screw and the corresponding slide base 26 in the device of the present invention.
FIG. 1 is a cylinder, 11 is a case, 13 is a slide holder, 15 is a slider, 17 is a screw shaft,
18 is a right-hand threaded part, 19 is a left-hand threaded part, 20 is a knob,
22 and 23 are slide screws, 24 and 25 are arms, 26 is a slide base, 27 is a syringe pressing part, 28 is a syringe holder, and 31 is a test tube holder.

Claims (1)

[Claims] 1. A case, a slide holder fixed to the case, a slider held by the slide holder so as to be able to move up and down, and a right-handed screw thread that is rotatably held by the slider and arranged almost horizontally, with a right-hand screw threaded around the center of the case. A screw shaft with a left-hand threaded portion and a knob fixed to one end, a slide screw screwed into each threaded portion of the screw shaft, and a slide screw whose upper end is rotatably connected to a slide holder or case and whose lower end is a slide screw. Extrusion of a separated sample comprising: a pair of arms rotatably connected to the slider; a slide base connected to the lower end of the slider and having a cylinder pressing part; and a syringe holder located below the slide base and fixed to a case. Device.