JPS6038372B2 - How to prevent and treat dog diarrhea - Google Patents

Abstract

1503094 The prophylaxis and treatment of diarrhoea in dogs NISSHIN FLOUR MILLING CO Ltd 7 April 1976 [9 April 1975] 14161/76 Heading ASB Diarrhoea in dogs is prevented and treated by orally administering to dogs Bifidobacterium pseudolongum and/or Bifidobacterium adolescentis isolated from the intestines and/or faeces of dogs. The bifidobacteria may be administered as a food composition obtained (1) by mixing them with a dog food, (2) dispersing them in sterilized milk or (3) by mixing them into a gelatinized starch paste containing an amino acid vacuum drying the mixture and then crushing it to granules. Veterinary compositions comprise (1) the above Bifidobacterium pseudolongum and/or Bifidobacterium adolescentis and (2) a dog food, sterilized milk or an amino acid e.g. sodium glutamate, lysine hydrochloride and/or 1-cysteine.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for preventing the occurrence of diarrhea and treating diarrhea, and more specifically, the present invention relates to a method for preventing the occurrence of diarrhea and treating diarrhea, and more specifically, the present invention relates to a method for preventing the occurrence of diarrhea and treating diarrhea. The present invention relates to a method for preventing and treating major diarrhea by orally administering one or more species of U. adrecentis to dogs.

Conventionally, antibacterial substances commonly used in humans have been used to treat large-scale diarrhea, but there are problems such as the spread of resistant bacteria and side effects.
Safe prevention and treatment methods for diarrhea are required, but currently there is no definitive method for prevention.

The present inventors completed the present invention by conducting bacteriological research on major diarrheal diseases and confirming that bacteria of the genus Bifidobacterium, which are resident bacteria in the intestine, are closely related to diarrheal diseases. I ended up doing it.

In other words, by administering large amounts of Bifidobacterium resident in the intestine, not only the number of contributing bacteria increases, but also the bacteria that grow abnormally during diarrhea is reduced, and the normal bacterial population returns to cure the diarrhea. This is what I found. The method of the present invention involves orally administering to a dog one or more types of bacteria resident in the intestine of large humans, such as Hyphidobacterium pseudolongum and Hyphidobacterium adolescentis.

Pifidobacterium pseudolongum used here is type b, and Bifidobacterium adrecenteis is types b, c, and d, and the dosage is at least once a day for prevention, and for treatment. In the case of 1 pi or more, a remarkable effect appears. These bacteria are originally colonized in the intestinal tract of healthy people, and no toxicity is observed when administered orally.

For example, when these freeze-dried bacteria were administered to mice, L was 2 females/k9 or more, which was the maximum dose that could be administered. The form of the bacteria to be administered is not particularly limited, such as dry preparations or liquid preparations, and it may be mixed with feed and given.

However, since the genus Bifidobacterium is an anaerobic bacterium, special consideration must be given to resistance to enzymes. For example, according to the method of Patent No. 645552 owned by the present applicant, It is possible to obtain stable formulations. It is also effective to add bacteria-promoting substances during formulation. Next, the effects of the present invention will be explained by giving specific examples.

Table 1 shows an example of administering one dose each of Bifidobacterium pseudolongum types b, c, and d and Pifidobacterium adolescentis type b to a dog exhibiting diarrhea symptoms. Shown below.

*As shown in Table 1, administration of Bifidobacterium leads to an increase in the bacteria themselves)
Both studies show that bacteria that specifically increase during diarrhea naturally decrease and return to normal flora.

Next, dogs exhibiting diarrhea symptoms were selected and examined for the presence of parasites. If positive, they were dewormed. Dogs exhibiting diarrhea for three days or more were targeted.

Table 2 shows the results of administering Bifidobacterium to this. Table 2 (Note) The numbers in the table indicate the number of large animals with diarrhea.

As mentioned above, Bifidobacterium that comes to animals other than large animals not only has almost no effect, but also has a dosage of 1.
A specific effect has been observed in both cases and above. Next, the puppies at 30 days old were separated from their mother dogs, fed with milk and commercially available dog food as baby food, and immediately after weaning, Bifidobacterium was administered. The results are shown in Table 3.

As shown in Table 3 and above, Bifidobacterium derived from animals other than Bifidobacterium are ineffective, and doses below 1 or less are less effective.

Example 1 Bufidobacterium pseudolongum isolated from a large stool sample was cultured anaerobically in Brix medium for 3702 q hours, and then collected by centrifugation.

The collected bacterial cells were M/151 containing L-cysteine.
Disperse the acid buffer at a volume equal to 1 volume per bacterial cell, and then add the following protective glue equivalent to 5 volumes of bacterial cells and disperse uniformly. Protective glue composition potato starch
5 Sodium glutamate 10 L cysteine 19g gelatin
15gM/5 phosphate buffer
500' and add 70% of the total amount of wheat starch.

The fungal paste solution prepared in this way is poured into a tray to form a flat plate, dried at 370 for 2 hours, and crushed with a mixer to form granules. When the preparation thus obtained was used to treat major diarrhea, the results shown in Table 4 were obtained.

Table 4
-1. Regular stool As mentioned above, the present invention was extremely effective against severe diarrhea.

Example 2 Bifidobacterium pseudolongum and Bifidobacterium adolescentis isolated from dog feces are separately cultured in a Brix medium anaerobically at 370°C for 20 hours and collected by centrifugation.

Claims (1)

[Claims] 1. A method for preventing and treating diarrhea, which comprises orally administering to a dog Bifidobacterium pseudolongum or/and Bifidobacterium adolescentis collected from the dog's digestive tract or feces.