JPS59214765A - Plasma separator and use thereof - Google Patents

Abstract

PURPOSE:To separate plasma from a small amount of a blood handily while compacting the equipment by employing a cylinder filled with a filter material. CONSTITUTION:A cylinder 2 made of plastics with a bottom plate 3 is placed on a transparent glass plate 1 and filled with a filter material 4 in a layer. The back of the bottom plate 3 is made higher in the smoothness to fully reflect an incident light. A whole blood dropped into a sunk portion 4a for storing blood in several drops flows downward based on own gravity and the capillary phenomenon of the filter material 4. The filter is formed coarse in the upper layer and close in the lower layer so that blood cells are retained in the upper layer while the plasma passes through a drilled hole 3a and expands in a void 5 as thin film. The separation of the plasma can be done handily without use of any large centrifugal separator or the like.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a plasma separator for separating whole blood and plasma portions, and a method for using the plasma separator for measuring the concentration of a test component in the plasma portion.

Conventionally, plasma separation has used costly equipment such as centrifugal separators, but these have disadvantages such as being difficult to operate and requiring a relatively large amount of blood. There was a need for a method to separate plasma from a small amount of blood.

The present invention has been made in view of the above circumstances, and the device is compact and easy to operate, and the device of the present invention can be used for separation instead of using a method that requires only a small amount of whole blood. The object of the present invention is to provide a plasma separation device which has the unique advantage of being able to measure the concentration of a specific component contained in a pooled plasma portion as it is.

In the device of the present invention, a metal bottom plate having a plurality of perforations is disposed on the surface of a colorless and transparent glass plate having a predetermined thickness with a slight gap between the glass plate and the bottom plate. It is constructed by placing a cylindrical body filled with F material for blood cell plasma separation on the upper part of the cylinder, and the plasma part separated from the blood flowing down from the upper part of the filled material is stored in the space as a thin film. It is something you are forced to do.

In addition, as a method of using the device of the present invention, light having the same wavelength as the absorption wavelength of the test component in the plasma portion, which is incident on the thin film from the back surface of the glass plate, is totally reflected on the back surface of the metal bottom plate. The concentration of the component to be detected is determined from the intensity I of the reflected light detected by the detector.
It is used to find C.

Hereinafter, the present invention will be explained based on embodiments with reference to the drawings. Figure 1 is a cross-sectional explanatory diagram showing the apparatus of the present invention.

A cylindrical body 2 having a bottom plate 3 is placed on a surface la of a colorless and transparent glass plate l having a predetermined thickness, for example, about 3 mm. The cylindrical body 2 is preferably made of a glass material such as polyvarnish, chill, polyethylene, etc. Inside the cylindrical body 2, a P material 4 for separating the sample blood into a blood cell portion and a plasma portion is placed on top thereof to form a blood pool. It is filled in layers forming a hemispherical depression 4a. The door material is laminated relatively loosely in the upper layer and relatively densely in the lower layer so that blood can be passed through smoothly. Next, the bottom plate 3 may be smooth on both the front and back, and in particular, it is preferable that the back surface is smooth because it totally reflects the incident light. As shown in FIG. 2, a plurality of perforations 3a are provided, and the fractionated plasma portion 7 passes through the perforations and forms a thin film in the small gap 5 formed between the bottom plate 3 and the glass plate l. It is something that can be stored.

Since the plasma separator according to the present invention has the above-described configuration, the whole blood that is dropped in units of several drops into the depressed portion 4a, where blood cells accumulate, gradually collects due to its own weight and the capillary action of the door material 4. It flows downward, but at this time, since the upper layer of the P material is coarse and the lower layer is dense, the blood cells stay in the upper layer, and the plasma finally passes through the perforation 3a and forms a thin film in the gap 5. It begins to spread. The thickness of the void is set in advance to be approximately several micrometers. In this way, it is possible to easily separate plasma without using conventional centrifugal separators, etc., which cost a lot of money, and since the structure is relatively simple, the manufacturing cost is also low. It is extremely useful as a blood test device as it is compatible with Sidi Spodeple products.

Next, a unique method of using the device of the present invention will be explained.

FIG. 3 is an explanatory view showing the state of use, and plasma has already been stored in the gap between the surface 1a of the glass plate 1 and the bottom plate 3 in the form of a thin film 10. Light having the same wavelength as the absorption wavelength of the test component (for example, bilirubin) in the plasma portion is refracted and transmitted through the glass plate 1 and the thin film 10, but is totally reflected by the metal bottom plate 3 and passes through the thin film 10 and the glass plate again. 1 and its strength can be detected by the detector 6.

Now, if the angle of incidence θ and the thickness D of the thin film are kept constant, the optical path length (d; α + β) of the light that passes through the thin film (in this case, it is treated as a cell) is determined, so Lambertian It is derived from Beer's formula % formula % (where ■ is the intensity of reflected light, ε is the molecular extinction coefficient d is the optical path length), and the purpose can be achieved.

The method of the present invention can measure the concentration of various plasma components by combining the device of the present invention with a reflection spectrophotometer. It is useful to apply to lines.

[Brief explanation of the drawing]

FIG. 1 is a sectional view showing a plasma separation device according to the present invention, and FIG.
The figure is a plan view showing the bottom plate portion of this device. 1...Glass plate, la...Glass plate surface, 2...
- Cylindrical body, 3... Bottom plate, 3a... Perforation, 4... Furnace material, 4&... Decave part, 5... Void, 6... Detector, 10... Thin film of plasma part . Patent applicant: Mochida Pharmaceutical Co., Ltd. Patent attorney Masanori Kai

Claims (2)

[Claims] (1) A metal bottom plate with a plurality of perforations is disposed on the surface of a colorless and transparent glass plate having a predetermined thickness with a slight gap between the glass plate and the top of the bottom plate. A cylindrical body filled with P material for separating blood cells and plasma is placed on the cylindrical body, and the plasma portion separated from the blood flowing down from the top of the filled P material is stored in the gap as a thin film. Plasma separation device 0 characterized by (2) A metal bottom plate with a plurality of perforations is disposed on the surface of a colorless and transparent glass plate having a predetermined thickness with a slight gap between the glass plate and the bottom plate. It is constructed by placing a cylindrical body to be filled with F U' 1≦ for separating blood cells and plasma, and the plasma 11ls separated from each door from the filled blood flowing down from the upper part of the fcP material is stored in the void as a thin film. Using a plasma separator equipped with a plasma separator, the light that is incident on the thin film from the back surface of the glass plate and has the same wavelength as the absorption wavelength of the test component in the plasma portion is totally reflected on the back surface of the metal bottom plate. Let it pass through the thin film again.
From the intensity I of the reflected light detected by the rztector, the concentration C of the test component can be calculated using the formula % (where ε is the molecular extinction coefficient and d is the optical path length). A method of using a plasma separator characterized by: