JPS59210343A - Automatic separation and collecting method of serum and its apparatus - Google Patents

Abstract

PURPOSE:To prevent the intrusion of dust and microbes in the atmosphere and blood plates into serums by collecting the serums into a collecting tube by centrifugal force without exposing aperture parts of a whole blood tube and a serum collecting tube to the atmosphere. CONSTITUTION:A whole blood tube 1, wherein a specified amt. of blood is charged, and an empty serum collecting tube 2 are inserted respectively into holders 3 and 4 which are inserted into a rack case 5, and the aperture parts are closed tightly by setting a collecting cap 11. Then after the bite of a lock plate 27 with a hook 28 is released, a rotor 7 is rotated by driving a motor 31. Then a bucket 8 is inclined horizontally due to centrifugal force, and the blood to be separated is separated into blood plates at the bottom part and serums at the upper part. After separation, by tilting the whole blood tube 1 and the serum collecting tube 2 slightly to a rotary shaft 26 side, and rotating the rotor 7 again by the motor 31, the serums as a supernatant liquid is collected in the serum collecting tube 2 from a separated liquid guide 24.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method and an apparatus for automatically separating serum from blood by centrifugation without manual intervention and without exposing it to the atmosphere. Inside the nokkesoto, which is swingably suspended, all blood vessels injected with blood are placed inside the radial direction of the locus, and serum collection tubes are placed outside, and both blood vessels are placed at the openings of these tubes. A separation cap having a separation liquid guide groove communicating with the openings of the tube is airtightly fitted, and the rotor is rotated to separate the blood in all the blood vessels into the serum and the face plate, and then the rotor is stopped. Rotate the rotor again by slightly tilting the bottom of the packet vertically or in the direction of the rotational axis of the rotor, and the centrifugal force will separate the serum in all blood vessels from the separation liquid guide groove of the collection cap into the serum collection tube. and a packet swingably suspended from the rotor, a bucket removably provided in the bucket, and a whole blood vessel opened on two sides, and a bucket outside the whole blood vessel in the bowsoto. A serum collection tube 6J that is freely insertable and removable in the radial direction of the rotor and has an open top surface, and the openings of all blood vessels and serum collection tubes are sealed;
and a bucket tilting mechanism consisting of a collection cap having a separated liquid guide groove in which the openings of both tubes communicate with each other, and a tournoid, an air cylinder, etc. for tilting the lower side of the packet in the direction of the rotational axis of the rotor. It is characterized by consisting of.

DESCRIPTION OF THE PREFERRED EMBODIMENTS Examples of the automatic serum dispersion separation method and device according to the present invention will be described below with reference to the drawings.

A full-face tube 1 into which a predetermined amount of blood a has been injected and an empty serum collection tube 2 are inserted into a whole blood vessel holder 3 and a serum collection tube holder 4, respectively, and each of these holders 3.4 is attached to a rack case 5. inserted into.

The upper surface of each holder 3.4 inserted into the rack case 5 has a separation liquid guide groove 1.0 in which the upper surface openings of the full-surface tube 1 and the serum collection tube 2 communicate with each other, and 1.2 is fitted with a collection camp 11 whose upper part is sealed.

This rack case 5 is fitted into a rotor 7 of a centrifugal separator 6 and a packet 8 that is swingably suspended from a suspension groove 39, and its upper surface is pressed and fixed by a plate 9.

Here, as the full-face tube 1, a test tube-like bottle with an open top is used, and as the serum collection tube 2, one having the same shape as the full-face tube 1 but slightly shorter and smaller is used.

The whole blood vessel holder 3 and the serum collection tube holder 4 are of the same shape and size, and both have four tube body insertion/removal holes 12.13 at equal intervals so that the head of each tube 3.4 protrudes. The top of the serum collection tube 2 is formed so as to protrude slightly upward from the top of the whole blood vessel 1, and the bottom is provided with a spring 1.4.15 for holding the tube. is interposed.

A pair of holder insertion parts 16.17 are formed in the radial direction of the rotor 7 during use of the rack case 5, and a fitting groove 1.8.19 of the distribution gap 11 is formed on the upper edge of the insertion part 16.17. are formed, and flanges 20 and 21 are formed on the upper end of the rack case in the opposing radial direction so as to facilitate gripping when the rack case 5 is inserted into and removed from the packet 8. The separation cap 11 is attached to the upper part of the facing full-face tube 1 and serum collecting tube 2 through a separation liquid guide 10 whose openings communicate with each other. silicone rubber formed with fitting parts 22 and 23 that are respectively sealed;
The aliquoting liquid guide groove 10 is made of a flexible material such as polycarbonate, and the bottom surface thereof is inclined from the upper edge of the serum collection tube 2 to the upper edge of the entire blood vessel 1 at a guide portion 24. is formed. Further, the lower peripheral edge of the fitting portion 22, 23 is formed with stepped portions 22a and 23a that come into contact with the upper peripheral surfaces of the full-surface tube 1 and the serum collection tube 2.

The bucket l-8 has a hook 28 that is engaged with a lock plate 27, which will be described later, at a slight inclination, at the lower end of the rotor 7 on the rotating shaft 26 side, on a surface perpendicular to the hanging shaft 25. Slide grooves 2 of 1/-1-9 are provided diagonally downward and on both inner surfaces of the upper side of the surface where the hanging shaft 25 is provided.
9.3o will be established by us.

The rotating shaft 26 of D-1-7 is the rotating shaft of the motor 31 (
(not shown), and is directly connected to the motor fixing base 32.
A disk-shaped locking plate 27 having a pot-shaped peripheral edge is attached to the bearing 33 of the rotating shaft 26 fixed above so as to be movable up and down.

That is, the spiral spring 34 is attached to the outer periphery of the bearing 33.
A spring case 35 is installed on the spiral spring 34 so as to be movable up and down, and a lock plate 27 is attached to the outer periphery of the spring case 35 via a bearing 36.

The upper circumferential edge of the lock plate 27 is configured to have a size such that it is positioned directly below the hook 28 when the rotor 7 is stopped, that is, when the packet 8 is suspended vertically, and the upper peripheral edge of the lock plate 27 A ring-shaped suction plate 38 is fixed at a position opposite to a ring-shaped solenoid 37 fixed to the motor fixing head 32.
Therefore, due to the excitation of the solenoid 37, the adsorption plate 38 resists the elasticity of the spiral spring 34 and moves the solenoid 3
7, the lock plate 27 descends, the packet 8 is disengaged from the hook 28, the solenoid 37 is demagnetized, the attraction plate 38 is lifted up by the elasticity of the spiral spring 34, and the lock plate 27 rises. , is engaged with the hook 8 of the packet 8, and the lower side of the packet 8 is slightly tilted toward the rotation axis 26 of the rotor 7.

In the figure, 40 indicates a chamber of the centrifuge 6.

With the automatic serum fraction collection device configured in this way,
To explain how to separate and collect serum, first, insert the whole blood vessel 1 into which the blood to be separated a has been injected into the tube insertion hole 12 of the whole blood vessel holder 3, and then insert the whole blood vessel 1 into which the blood to be separated a is injected. An empty serum collection tube 2 is inserted into the body insertion/removal hole 13.
These whole blood vessel holder 3 and serum collection tube holder 4 are each set as a pair, and the rotor 7
After positioning the whole blood vessel holder 3 on the rotating shaft 26 side and the serum collection tube 4 on the outside thereof and inserting it into the holder insertion part 16.17 of the rack case 5, insert the insertion groove 18.1 from above.
9 is fitted with a collection cap 11 to seal the openings of each full-face tube 3 and serum collection tube 4.

This rack case 5 is attached to the whole blood vessel holder 3 in the same manner as above.
Te packet 8 with its side located on the rotating shaft 26 side of rotor 7
insert the plate 9 into the slide grooves 29 and 30 of this packet 8 to press the top surface of the collection camp 11,
To prevent each of the above instruments inserted into the packet 8 from flying out from inside the packet 8 during centrifugal rotation.

Packet 8 is on the whole blood vessel Porg 3 side, i.e. hook 2
The hanging shaft 25 is loosely fitted into the hanging groove 39 of the rotor 7 with the side provided with the rotor 7 facing the rotating shaft 26 of the rotor 7, and the hanging shaft 25 is hung to complete the set.

Next, the solenoid 37 is energized, the suction plate 38 of the lock plate 27 is attracted, and the motor 31 is rotated with the lock plate 27 and the hook 28 disengaged.
When rotated, the packet 8 is tilted from a vertical position to a horizontal position by centrifugal force.

That is, as shown in Figure 9, the full-face tube 1 and the serum collection tube 2 are also tilted from the vertical position N (a) to the horizontal position (b), and the blood to be separated in the full-face tube 1 is separated by centrifugal force. The serum C is separated into two parts by the bottom faceplate.

After the separation is completed, the rotation of the motor 31 is stopped, and the rotation of the rotor 7 is stopped. After returning the entire blood vessel 1 and serum collection tube 2 to the vertical position (c), the solenoid 37 is turned on. The lock plate 27 is lifted up by the elasticity of the viral spring 34, and its upper edge is attached to the packet 8.
The bottom of the packet 8 is slightly tilted toward the rotating shaft 26 of the rotor 7.

That is, the full surface tube 1 and the serum collection tube 2 are in the vertical position ii'? (
c), the bottom part is tilted toward the rotating shaft 26 side of the rotor 7).

When the rotor 7 is rotated again by the motor 31 in this state, the supernatant liquid serum C in the full-surface tube 1 is separated into the serum collection tube 2 through the separation liquid guide groove 1o.
At this time, since the lock plate 27 and the hook 28 are engaged, the lock plate 27 is also rotated as the packet 8 rotates, but the lock plate 27 is attached to the spring case 35 via the bearing 36. Therefore, the lock plate 27 rotates smoothly together with the flea cerato 8.

After the serum C has been collected, the rotation of the motor 31 is stopped, the rotation of the rotor 7 is stopped, the solenoid 37 is energized, the engagement between the lock plate 27 and the hook 28 is released, and the bucket 8 is vertically moved. Then, the full surface tube 1 and the serum collection tube 2 also return to the vertical position (E), and if serum C exceeding the capacity of the blood vessel 2 has been collected on the serum collection tube 2 side, the excess serum C is collected. is naturally refluxed into the entire surface tube 1 from the guide portion 24 of the collection cap 11 due to the drop, and the collection of serum C is completed.

After this separation of serum C is completed, contrary to the above-mentioned procedure, remove the bucket 8 from the rotor 7, remove the plate 9 from the bucket 8, take out the rack case 5 from the bucket 8, and remove the rack case 5 from the bucket 8. Remove the collection cap 11 from the blood vessel holder 3, remove the full-face tube 1 from the whole blood vessel holder 3, plate it, and discard the excess serum C. Take out the serum collection tube 2 from the serum collection tube holder 4 and use the serum C for the intended use. Serve. Rank case 5 is serum collection tube 2
Alternatively, the collection cap 11 can be used as a storage case for covering the serum collection tube 2 and storing it in a refrigerator or the like.

In the above-mentioned embodiment, a device is shown that can simultaneously separate and collect two sets of 4-to-1 sets, such as a full-face tube 1 and a serum collection tube 2, but even a 1-to-1 set can also be used for many-to-half sets. However, it goes without saying that the number does not matter, and there are 3 holders in the slot 8.
It is also possible to use a configuration in which the full-face tube 1 and the serum collection tube 2 are inserted directly into the bucket 8 without inserting the whole blood vessel 1 and the serum collection tube 2 through the rack case 4 and the rack case 5. , the no-kerato tilting mechanism also includes a solenoid 37, a suction plate 38,
In addition to the lock plate 27, the lock plate 2 can be tilted directly by using an air cylinder or the like, or by using an air cylinder instead of the solenoid 37.
It is obvious that the design can be changed as appropriate, such as by raising and lowering the 7.

The present invention separates blood into serum and a face plate using the above-described method and apparatus, and then automatically collects serum without exposing all blood vessels or the opening of the serum collection tube to the atmosphere. There is no risk that dust or bacteria floating in the air will be mixed into the serum during collection, and it is possible that all blood vessels should be vertically placed or their bottoms should be placed in the direction of the rotational axis of the rotor. The rotor is rotated at a slight tilt, and the centrifugal force is used to separate the serum into the serum collection tube.The serum collection tube is held almost vertically, and the supernatant liquid is collected while tilting all the blood vessels. With devices that separate serum into serum collection tubes, even a small amount may get mixed into the faceplate or the collection tube, and to avoid contamination of the faceplate, more serum than necessary is left in all blood vessels, resulting in wasted serum. Therefore, pure and high-quality serum separation can be automatically performed without waste.

Furthermore, in the apparatus according to the present invention, by configuring it as in the embodiment, the serum does not scatter outside the bottle when separating and collecting serum, and it is also convenient for storing serum. It has both effects.

[Brief explanation of drawings]

The drawings show an embodiment of the automatic serum separation and collection method and apparatus according to the present invention. The figure is a perspective view of the rack case, Figure 3 is a perspective view of the bucket, Figures 4 (a) and (b) are a plan view and sectional view of the collection cap, Figure 5 is a perspective view of the plate, and Figure 6 Figure 7 is a partially cross-sectional front view of the main part during serum separation, Figure 8 is a partially cross-sectional front view of the main part during serum separation, and Figure 8 is a bucket suspended from the rotor. FIGS. 9A to 9E are explanatory diagrams showing the state of serum fraction collection. 1... Full tube 2... Serum collection tube 7°°°
Rotor 8... Bucket 10... Separated liquid guide groove 11... Collection cap 26... Rotating shaft 37
... Solenoid B ... Face plate C ... Serum patent applicant Domestic Centrifuge Co., Ltd.

Claims (1)

[Claims] (1) In a packet that is swingably suspended in a rotor, all blood vessels into which blood is injected are arranged inside the rotor in the radial direction, and serum collection tubes are arranged outside, A collection cap with a separating liquid guide groove that communicates with the openings of both tubes is airtightly fitted to the openings of both tubes, and the rotor is rotated to separate the blood in all blood vessels into serum and the face plate. After that, the rotor is stopped, the bottom of the packet is slightly tilted vertically or in the direction of the rotation axis of the rotor, and the rotor is rotated again, and the centrifugal force is used to collect the serum in all blood vessels. An automatic serum separation and collection method characterized by separating serum into a serum collection tube from a groove. (2) A packet that is swingably suspended from a rotor, a whole serum that is removably inserted into the packet and has an open top surface, and a radiator of the rotor outside of all the blood vessels inside the packet. A serum collection tube that is freely insertable and removable in the direction and has an open top surface; Automatic serum separation comprising a collection cap having a liquid guide and a packet tilting mechanism comprising a solenoid-air cylinder or the like for tilting the lower side of the packet in the direction of the rotational axis of the rotor. Separation device. (3) The opening of the serum collection tube is provided at a higher position than the openings of all blood vessels, and the lower side of the separation liquid guide groove is on the line connecting the opening edges of all blood vessels and the opening edges of the serum collection tube. The automatic serum separation and collection device according to claim (2), which is formed to be inclined to. (4.) The insertion/removal holes for all the blood vessels and the insertion/removal hole for the serum collection tube formed in the packet are equipped with springs or other elastic materials to constantly push all the blood vessels and the serum collection tube upward. The automatic serum separation and collection device according to any one of claims (2) and (3), wherein the automatic serum separation and collection device has an elastic mechanism for insertion and removal, and the collection cap is made of flexible material such as polyethylene or silicone rubber. (5) A packet according to any one of claims (2) to (4), wherein the packet is constituted by a rank into which all blood vessels and serum collection tubes are inserted and removed, and a keratin into which the rank is inserted and removed. Automatic serum separation and collection device.