JPS59192938A - Sample box with automatic rotating polarizing plate - Google Patents

Abstract

PURPOSE:To control an analyzer deflecting plate with high precision and vary a measurement time upon occasion by leading out polarized light parallel to excitation light and polarized light perpendicular to the excitation light under the control of a stepping motor. CONSTITUTION:White light L1 emitted by a nanosecond light source 1 has the quantity of light adjusted by a diaphragm 2, a filter 3 selects only light with wavelength for exciting a sample, and a polarizing plate 4 of a polarizer irradiates a sample solution fixed in a sample cell 5 with only linear polarized light L2. Fluorescent light L3 emitted by the sample is incident to a polarizing plate 9 of an analyzer and rotated at 90 deg. angle intervals by the stepping motor 10, so that a detector 6 measures the polarized light parallel to the linear polarized light of the excitation light and polarized light perpendicular to it as to the intensity at predetermined intervals of time. This stepping motor is driven by following an optional procedure by inputting desired data by a control circuit.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of Industrial Application The present invention is directed to a sample box of an analyzer that utilizes fluorescence depolarization to investigate minute rotational movements of nanoseconds such as polymers in a solution. This was bad for new improvements, and the accuracy of the rotation angle of the polarizing plate deteriorated, resulting in poor data reliability.

It's targeted.

Aperture bar for adjustment 3 is a filter for extracting the monochromatic light that passes through the aperture 2 and detects the fc source, 4 is a nyc polarizer polarizing plate that is in close contact with the filter 3, and 5 is a sample cell made of quartz glass or the like. A desired sample solution (not shown) is stored in the hollow portion 5cL of the cell 5, passes through the polarizing plate 4, and is irradiated with brightly polarized light L2.6.
is the fluorescent light emitted from the sample L3i filter 7, aperture 8
A detector, 9, consisting of a photomultiplier tube, receives via
The analyzer polarizing plate 10 located immediately in front of the filter 7 is a step motor for rotating the polarizing plate 9 by a predetermined amount through a rotation axis 10α. It's like being driven by X.

In addition, as shown in FIG.
It is connected to 0α.

Effect of the Invention The white light L1 emitted from the nanosecond light source 1 is adjusted in light intensity by the aperture 2, and only the light having a wavelength that excites the sample is selected by the N1 filter 3.Only the line-side light L2 is selected by the polarizer polarizing plate 4. In cell 5, the fixed sample solution is irradiated. The fluorescent light L3 emitted from this sample enters the analyzer polarizing plate 9, which is rotated by a step motor 10 by 90 degrees to separate polarized light parallel to and perpendicular to the line side light of the excitation light. The detector 6 measures the intensity at predetermined time intervals. The step motor is driven in an arbitrary manner by inputting desired data to a control circuit (not shown).

Effects of the Invention Since the present invention uses a step motor as described above to rotate the analyzer polarizing plate, (1) the analyzer polarizing plate can be controlled with high precision without integrating each rotational error.

(21) The rotation speed and period of rotation can be controlled by the number of signal pulses per second entering the Luss motor and the frequency, so the measurement time can be changed according to the needs of the foot measurer.

(3) Fully automated control using a computer makes it possible to increase the efficiency of time-consuming experiments.

There are significant effects such as

[Brief explanation of the drawing]

1 and 2 are a plan view of the apparatus according to the present invention and a plan view of only the analyzer polarizing plate. In Figure 1, 1 is a nanosecond light source that emits light in a wide range from the ultraviolet region to the visible light region, 2 is an aperture, 3 is a filter, 4 is a polarizer polarizing plate, 5 is a sample cell, and 5α is a sample cell. 6 is a detector consisting of a photomultiplier tube;
7 is an aperture, 8 is a filter, 9 is an analyzer polarizing plate, 1
0α is a step motor, a support for connecting 10 and the analyzer polarizing plate 9, and 10 is a step motor for rotating the analyzer polarizing plate. Applicant Daini Seikosha Co., Ltd. Agent Patent Attorney Mogami Figure 1 Figure 2

Claims (1)

[Claims] A nanosecond light source extracts linearly polarized light from its light, and a polarizer polarizer and its linearly polarized light extract the fluorescent light parallel to the excitation light from the quartz glass sample cell that holds the excited sample and the sample. This sample box consists of an analyzer polarizing plate that extracts polarized light perpendicular to the polarized light, and a photomultiplier tube detector that measures the total intensity of each polarized light.It is controlled by a step motor to detect polarized light parallel to the n1 excitation light and perpendicular to the excitation light. It is characterized by being able to take out polarized light alternately.
Polarizing plate automatic rotating sample box.