JPS59141055A - Modified electrode - Google Patents
Modified electrodeInfo
- Publication number
- JPS59141055A JPS59141055A JP57196608A JP19660882A JPS59141055A JP S59141055 A JPS59141055 A JP S59141055A JP 57196608 A JP57196608 A JP 57196608A JP 19660882 A JP19660882 A JP 19660882A JP S59141055 A JPS59141055 A JP S59141055A
- Authority
- JP
- Japan
- Prior art keywords
- electrode
- transport substance
- nitrogen
- amount
- saturation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 16
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229910002804 graphite Inorganic materials 0.000 claims abstract description 13
- 239000010439 graphite Substances 0.000 claims abstract description 13
- 239000007787 solid Substances 0.000 claims abstract description 9
- JZMJDSHXVKJFKW-UHFFFAOYSA-M methyl sulfate(1-) Chemical compound COS([O-])(=O)=O JZMJDSHXVKJFKW-UHFFFAOYSA-M 0.000 claims abstract description 6
- 108091006149 Electron carriers Proteins 0.000 claims description 17
- ROFVEXUMMXZLPA-UHFFFAOYSA-N Bipyridyl Chemical compound N1=CC=CC=C1C1=CC=CC=N1 ROFVEXUMMXZLPA-UHFFFAOYSA-N 0.000 claims description 4
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 claims description 4
- 239000000126 substance Substances 0.000 abstract description 16
- 239000013543 active substance Substances 0.000 abstract description 3
- 238000003411 electrode reaction Methods 0.000 abstract description 3
- 229920000642 polymer Polymers 0.000 abstract description 2
- 238000010828 elution Methods 0.000 abstract 2
- 230000003100 immobilizing effect Effects 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 description 7
- 230000000975 bioactive effect Effects 0.000 description 7
- 229910052799 carbon Inorganic materials 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 108010052832 Cytochromes Proteins 0.000 description 4
- 102000018832 Cytochromes Human genes 0.000 description 4
- 102000001554 Hemoglobins Human genes 0.000 description 4
- 108010054147 Hemoglobins Proteins 0.000 description 4
- 239000000975 dye Substances 0.000 description 4
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 4
- 239000003929 acidic solution Substances 0.000 description 3
- 230000002378 acidificating effect Effects 0.000 description 3
- 125000003118 aryl group Chemical group 0.000 description 3
- 239000003729 cation exchange resin Substances 0.000 description 3
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 description 2
- 229930002875 chlorophyll Natural products 0.000 description 2
- 235000019804 chlorophyll Nutrition 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
- 241000192537 Anabaena cylindrica Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 description 1
- 229910052770 Uranium Inorganic materials 0.000 description 1
- 229940088623 biologically active substance Drugs 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229940023913 cation exchange resins Drugs 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 108010008495 collagen-glycosaminoglycan copolymer Proteins 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 108010043199 cytochrome c(3) Proteins 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- FIKAKWIAUPDISJ-UHFFFAOYSA-L paraquat dichloride Chemical compound [Cl-].[Cl-].C1=C[N+](C)=CC=C1C1=CC=[N+](C)C=C1 FIKAKWIAUPDISJ-UHFFFAOYSA-L 0.000 description 1
- 229920002717 polyvinylpyridine Polymers 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/333—Ion-selective electrodes or membranes
- G01N27/3335—Ion-selective electrodes or membranes the membrane containing at least one organic component
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nanotechnology (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Electrochemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Composite Materials (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Materials Engineering (AREA)
- Crystallography & Structural Chemistry (AREA)
- Molecular Biology (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は、導電性固体電極の表面を修飾した新規な電極
及びその用途に関する。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a novel surface-modified conductive solid electrode and its uses.
従来、導電性固体電極の表面を、重合体状の電子伝達体
で修飾した電極は公知であるが、そのような電極は、重
合度によるバラツキが生じやすり、シかも使用に際して
強酸性溶液を必要とする欠点があった。Conventionally, electrodes in which the surface of a conductive solid electrode is modified with a polymeric electron carrier are known, but such electrodes tend to vary depending on the degree of polymerization and require a strong acidic solution for use. There was a drawback.
パイロリティックグラファイトのうえに、ポリビニルピ
リジン等を固定化した電極が知られている（Ｎ、 Ｏｙ
ａｍａ ｅｔ ａｌ、 Ｊ。Electrodes in which polyvinylpyridine, etc. are immobilized on pyrolytic graphite are known (N, Oy
ama et al, J.
Ｅ Ｉｅｃｔｒｏｃｈｅｍ、 Ｓ ｏｃ、 、 １２
７． Ｇ４０ （１９Ｈ）など〉が、強酸性溶液下で
のみ使用可能であり、また固定化される物質が陽イオン
系のみに限られるという欠点を持つ。E Electrochem, Soc, , 12
7. G40 (19H), etc.) have the disadvantage that they can only be used in strongly acidic solutions and that the substances that can be immobilized are limited to cationic substances.
また、メチルビオロゲンのような電子伝達体を、溶液ま
たは懸濁液の形で、化学修飾を施し、または施していな
い電極と一緒に使用づる方法も知られている。しかし、
その場合には、多量の電子伝達体を必要とし、電極反応
に関与しない物もあるので効率上不利である。It is also known to use electron carriers such as methyl viologen in solution or suspension form with electrodes with or without chemical modification. but,
In that case, a large amount of electron carriers are required, and some of them do not participate in the electrode reaction, which is disadvantageous in terms of efficiency.
本発明の目的は、これら従来技術の難点を解消し、製造
、取扱いが容易で、広範な応用面を持つ、新規な修飾電
極を提供するにある。It is an object of the present invention to provide a novel modified electrode that overcomes the drawbacks of these conventional techniques, is easy to manufacture and handle, and has a wide range of applications.
本発明者は、既に、導電性固体電極の表面に、芳香族基
含有強酸性陽イオン交換樹脂及び含窒素電子伝達体を固
定化したことを特徴とする電極及びそれを使用する生体
活性物質センサーその他を発明しており、その発明は、
芳香族基含有強酸性陽イオン交換樹脂を介在させれば、
含窒素電子伝達体が安定に固定化された修飾電極が得ら
れるという発見に基づくのである。（特願昭５７−３６
８７０＞。The present inventor has already disclosed an electrode characterized in that an aromatic group-containing strongly acidic cation exchange resin and a nitrogen-containing electron carrier are immobilized on the surface of a conductive solid electrode, and a bioactive substance sensor using the same. He has also invented other things, and his inventions are:
If a strongly acidic cation exchange resin containing an aromatic group is used,
This is based on the discovery that a modified electrode in which a nitrogen-containing electron carrier is stably immobilized can be obtained. (Special application 1986-36
870>.
本発明者は、更に研究の結果、そのような芳香族基含有
強酸性陽イオン交換樹脂を介在「ずに生体活性物質に応
答する含窒素電子伝達体としてビオロゲン色素、ビピリ
ジン、フェナントロリン及び１−メトキシ−５−メチル
フエナゼニウム メチル サルフェートのうちの少なく
とも１種を選び、これをグラファイト層状面（ｂａｓａ
ｌ ｐｌａｎｅ ） Ｂ出のパイロリティックグラフ
ァイトと組合わせることにより安定な化学修飾電極が容
易に得られ、ヘモグロビンなどのヘム蛋白質、ＮＡＤＨ
。As a result of further research, the present inventor found that viologen dye, bipyridine, phenanthroline, and 1-methoxy as nitrogen-containing electron carriers that respond to biologically active substances without intervening such aromatic group-containing strongly acidic cation exchange resins. Select at least one of -5-methylphenazenium methyl sulfate and apply it to a graphite layered surface (basa
A stable chemically modified electrode can be easily obtained by combining with pyrolytic graphite from B.
.
Ｎ Ａ Ｄ Ｐ ｌ−１などに優れた電気化学的応答を
示すことを見出し、このような発見に基づき本発明を完
成した。すなわち、本発明はグラフフィト層状面（ｂａ
ｓａｌ ｐｌａｎｅ ）露出のパイロリティックグラ
ファイトからなる導電性固体電極の表面に、ごオロゲン
色素、ビピリジン、フェナントロリン及び１−メトキシ
−５−メチルフエナゼニウム メチル ザルフェートか
らなる群より選ばれた含窒素電子伝達体を固定化した化
学修飾電極に関するものである。It was discovered that the present invention exhibits an excellent electrochemical response to N A D P l-1, etc., and the present invention was completed based on this discovery. That is, the present invention provides graphite layered surfaces (ba
A nitrogen-containing electron carrier selected from the group consisting of ologen dye, bipyridine, phenanthroline, and 1-methoxy-5-methylphenazenium methyl sulfate is applied to the surface of an exposed conductive solid electrode made of pyrolytic graphite (sal plane). This invention relates to a chemically modified electrode with immobilized .
本発明に使用する導電性固体電極は、従来公知のもので
よく、グラフフィトの層状面が多く露出しているパイロ
リティックグラファイト例えばＵ、Ｃ，Ｃ，製５ｔｒｅ
ｓｓ −ａｎｎｅａｌｅｄｐｙｒｏｌｙｔｉｃ ｇｒ
ａｐｈｉｔｅ （以下５ＡＰＧと略記する。）等の炭素
電極が例として挙げられる。The conductive solid electrode used in the present invention may be a conventionally known one, such as pyrolytic graphite with many graphite layered surfaces exposed, such as 5tre made of U, C, C, etc.
ss-annealedpyrolytic gr
Examples include carbon electrodes such as aphite (hereinafter abbreviated as 5APG).
含窒素電子伝達体としては、ビオロゲン色素、ビピリジ
ン類、フェナントロリン及び１−メトキシ−５−メチル
フエナゼニウム メチル サルフェートが用いられる。As the nitrogen-containing electron carrier, viologen dyes, bipyridines, phenanthroline, and 1-methoxy-5-methylphenazenium methyl sulfate are used.
本発明の修飾電極は、上記のようなグラファイト層状面
（ｂａ、ｓａｌ ｐｌａｎｅ ）の露出した炭素電極
を含窒素電子伝達体水溶液中に浸漬し必要により洗浄、
乾燥により容易に作成することができる。炭素電極表面
に固定化された含窒素電子伝達体の量は、単分子層（飽
和吸着量）が好ましいが、それ以下の量であっても実用
に供し得る。The modified electrode of the present invention is prepared by immersing a carbon electrode with an exposed graphite layer plane (BA, sal plane) in an aqueous solution of a nitrogen-containing electron carrier, cleaning it if necessary,
It can be easily created by drying. The amount of nitrogen-containing electron carrier immobilized on the carbon electrode surface is preferably a monomolecular layer (saturated adsorption amount), but even if the amount is less than that, it can be used practically.
本発明の電極の従来の電極に勝る利点としては、下記の
ものが挙げられる。Advantages of the electrode of the present invention over conventional electrodes include the following.
（１）炭素電極を含窒素電子伝達体水溶液中に浸漬する
だけでよく、作成が極めて容易であり、かつ、含窒素電
子伝達体の使用量が極めて少ない。(1) It is extremely easy to create the carbon electrode by simply immersing it in an aqueous solution of the nitrogen-containing electron carrier, and the amount of the nitrogen-containing electron carrier used is extremely small.
（２）電極反応に使用する溶液は中性付近、すなわちｐ
）−１４〜１０でよい。すなわち、従来の問題となる強
酸性溶液は不要であって、生体活性物質に応答する電極
に適する。(2) The solution used for the electrode reaction is near neutrality, that is, p
)-14 to 10 is sufficient. That is, there is no need for a strongly acidic solution, which is a problem in the prior art, and it is suitable for electrodes that respond to biologically active substances.
（３）電子伝達体は単量体でよく、しかも定量的に固定
化される。すなわち、重合体の調製、重合度の均質化を
必要としない。(3) The electron carrier may be a monomer and is quantitatively immobilized. That is, it is not necessary to prepare a polymer or homogenize the degree of polymerization.
（４）電子伝達体は単分子層で固定化され安定であるの
で、溶液または懸濁液の形で使用する場合よりも量が少
なくて済み、かつ効率がよい。(4) Since the electron carrier is fixed in a monomolecular layer and is stable, it requires less amount and is more efficient than when used in the form of a solution or suspension.
（５）適度に親水性でかつ溶出しないので、使用上便利
である。(5) It is convenient to use because it is appropriately hydrophilic and does not elute.
それ故、このような電極は、生体活性 物質に応答する電極として顕著に優れている。Therefore, such electrodes are bioactive It is outstandingly good as an electrode that responds to substances.
このような電極は、該含窒素電子伝達体と共役する生体
活性物質の存在により電流を発生させることができるの
で、バイオリアクターの電極として使用し得る。また、
そのとき該生体活性物質の温度と電流量とが直線的比例
関係にあるので、センサーとして使用され、生体活性物
質を定量することができる。Such electrodes can be used as electrodes in bioreactors since they can generate electrical current due to the presence of bioactive substances conjugated to the nitrogen-containing electron carrier. Also,
At this time, since the temperature of the bioactive substance and the amount of current are in a linear proportional relationship, it can be used as a sensor to quantify the bioactive substance.
すなわち、測定しようとする生体活性物質をクロマトグ
ラフなどで分別した後、ｐ ｉｆ ４〜１０の緩衝溶液
中に加え、一方に本発明による電極、他方に適当な電極
を設置して通電し、電流量を測定すると、生体活性物質
の濃度が測定できる。That is, after the biologically active substance to be measured is separated using a chromatograph or the like, it is added to a buffer solution with a pif of 4 to 10, an electrode according to the present invention is placed on one side, and an appropriate electrode is placed on the other side, and electricity is applied to the mixture. By measuring the amount, the concentration of the bioactive substance can be determined.
含窒素電子伝達体に応答する生体活性物質を例示すると
、ＮＡＤＨ，ＮＡＤＰＨ，チトクロムＣ３，チトクロム
Ｃ，ヘモグロビン。Examples of bioactive substances that respond to nitrogen-containing electron carriers include NADH, NADPH, cytochrome C3, cytochrome C, and hemoglobin.
クロロフィル等及びこれらを含む電子伝達系が挙げられ
る。Examples include chlorophyll and the like and electron transport systems containing these.
以下、本発明を実施例により具体的に説明するが、本発
明はこれらに限定されるものではない。EXAMPLES Hereinafter, the present invention will be specifically explained with reference to Examples, but the present invention is not limited thereto.
実施例１
直径５ｍｍの５ＡＰＧを１−メトキシ−５−メチルフエ
ナゼニウム メチル サルフェートの１ｍＭ水溶液中に
約１０秒間浸漬し、次いで洗浄、乾燥し修飾電極を作成
した。Example 1 5APG with a diameter of 5 mm was immersed in a 1 mM aqueous solution of 1-methoxy-5-methylphenazenium methyl sulfate for about 10 seconds, then washed and dried to prepare a modified electrode.
この電極を用い、ＮＡＤＨを電気化学的に酸化した。Ｎ
ＡＤＨのｍ度と電流量の関係を測定したところ、表１に
示すように直線的比例関係を示した。Using this electrode, NADH was electrochemically oxidized. N
When the relationship between the m degree of ADH and the amount of current was measured, a linear proportional relationship was shown as shown in Table 1.
実施例２
直径５＋ｎｍの５ＡＰＧをビオロゲン色素の１１ＴＩＭ
水溶液中に約１０秒間浸漬し、次いで洗浄、乾燥し修飾
電極を作成した。Example 2 5APG with a diameter of 5+nm was converted into viologen dye 11TIM
It was immersed in an aqueous solution for about 10 seconds, then washed and dried to produce a modified electrode.
この電極を用い、還元型ヘモグロビンを電気化学的に酸
化した。ヘモグロビンの濃度と電流量の関係を測定した
ところ、表２に示すように直線的比例関係を示した。Using this electrode, reduced hemoglobin was electrochemically oxidized. When the relationship between the concentration of hemoglobin and the amount of current was measured, a linear proportional relationship was shown as shown in Table 2.
実施例３
直径５ｍｍの５ＡＰＧをビピリジンの１ｍＭ水溶液中に
約１０秒間浸漬し、次いで洗浄、乾燥し修飾電極を作成
した。Example 3 A 5APG with a diameter of 5 mm was immersed in a 1 mM aqueous solution of bipyridine for about 10 seconds, then washed and dried to produce a modified electrode.
この電極を用い、還元型チトクロムＣを電気化学的に酸
化した。チトクロムＣの濃度と電流量の関係を測定した
ところ、表３に示すように直線的比例関係を示しＩＣ０
実施例４
実施例１と同様に作成した修飾電極をＮＡＤＨ，５０ｏ
／Ｊの水溶液に浸漬し、電流密度的１０μＡ１０（で通
電したところ、ＮＡＤをほぼ定量的に得た（収率的９０
％）。Using this electrode, reduced cytochrome C was electrochemically oxidized. When the relationship between the concentration of cytochrome C and the amount of current was measured, a linear proportional relationship was shown as shown in Table 3.
/J in an aqueous solution and energized with a current density of 10 μA (10 μA), NAD was obtained almost quantitatively (yield: 90 μA).
%).
実施例５
直径５ｍｍの５ＡＰＧを１ｍＭメチビオロゲン水溶液に
約１０秒間浸漬し、次いで洗浄、乾燥し修飾電極を作成
した。Example 5 5APG with a diameter of 5 mm was immersed in a 1 mM methiviologen aqueous solution for about 10 seconds, then washed and dried to prepare a modified electrode.
表１
ＮＡＤＨ濃度 ビーク電流
（ｇ／、ｅ＞ （μＡ）０．６８
１２．６
０．５０ ９．１
０．３５ ６．３
０．１７ ３．３
ｏ、 ｏ ｏ、 。Table 1 NADH concentration Peak current (g/, e> (μA) 0.68
12.6 0.50 9.1 0.35 6.3 0.17 3.3 o, o o, .
表２
ヘモグロビン ビーク電流
（ａｚｌ） （μＡ）
１２．９ ３．０
８、５ ２．０
６、５ １．５
３、３ ０．８
チトクロムＣビーク電流
（ｃ＋／Ｊ＞ （μＡ）８、０
９．１
６、０ ６．９
４・０ ４．６
２、０ ２．３
また蒸溜水２０ｍｇにポリビニルアルコール３ｇを加え
常温でよく分散させた。ゆっくり加熱しなからｓｍさせ
ない状態で完全に溶解させた後ゆっくり冷やし常温に戻
した。その後必要に応じて、牛血清アルブミンをポリビ
ニルアルコール重量当り１％加えて溶解させた。この溶
液にアナベナシリンドリ力
（ａｎａｂａｅｎａ ｃｙｌｉｎｄｒｉｃａ）懸濁液
（クロロフィル含量約３重量％）を０．２８０混合した
。Table 2 Hemoglobin peak current (azl) (μA) 12.9 3.0 8, 5 2.0 6, 5 1.5 3, 3 0.8 Cytochrome C peak current (c+/J> (μA) 8, 0
9.1 6.0 6.9 4.0 4.6 2.0 2.3 Furthermore, 3 g of polyvinyl alcohol was added to 20 mg of distilled water and well dispersed at room temperature. After heating slowly and completely dissolving the mixture without causing sm, it was slowly cooled to room temperature. Thereafter, if necessary, 1% bovine serum albumin was added and dissolved based on the weight of polyvinyl alcohol. To this solution was mixed 0.280 of anabaena cylindrica suspension (chlorophyll content about 3% by weight).
この溶液を用意した該修飾電極上に均一に薄く塗布し、
直ちに０〜５℃の暗所のデシケータ−に入れて乾燥した
。乾燥重量は２ ｍｇであった。Apply this solution thinly and uniformly on the prepared modified electrode,
It was immediately placed in a desiccator in the dark at 0 to 5°C to dry. Dry weight was 2 mg.
Claims (1)
）露出のパイロリティックグラファイトからなる導電性
固体電極の表面に、ビオロゲン色素、ビピリジン、フェ
ナントロリン及び１−メトキシ−５−メチルフエナゼニ
ウム メチル サルフェートからなる群より選ばれた含
窒素電子伝達体を固定化した化学修ｆｓ電極。1. Graphite layered plane (basal plane)
) A nitrogen-containing electron carrier selected from the group consisting of viologen dye, bipyridine, phenanthroline, and 1-methoxy-5-methylphenazenium methyl sulfate is immobilized on the surface of an exposed conductive solid electrode made of pyrolytic graphite. Chemically modified fs electrode.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57196608A JPS59141055A (en) | 1982-11-09 | 1982-11-09 | Modified electrode |
| US06/543,295 US4595479A (en) | 1982-11-09 | 1983-10-19 | Modified electrode |
| EP83306407A EP0109767B1 (en) | 1982-11-09 | 1983-10-21 | Modified electrode |
| DE8383306407T DE3377017D1 (en) | 1982-11-09 | 1983-10-21 | Modified electrode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57196608A JPS59141055A (en) | 1982-11-09 | 1982-11-09 | Modified electrode |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS59141055A true JPS59141055A (en) | 1984-08-13 |
Family
ID=16360579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57196608A Pending JPS59141055A (en) | 1982-11-09 | 1982-11-09 | Modified electrode |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS59141055A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090159449A1 (en) * | 2007-12-20 | 2009-06-25 | Canon Kabushiki Kaisha | METHOD FOR PRODUCING ELECTRODE HAVING IMMOBILIZED pi-CONJUGATED LIGANDS, ELECTRODE HAVING IMMOBILIZED pi-CONJUGATED METAL COMPLEXES, AND ELECTRODE HAVING IMMOBILIZED pi-CONJUGATED MULTINUCLEAR METAL COMPLEXES |
-
1982
- 1982-11-09 JP JP57196608A patent/JPS59141055A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090159449A1 (en) * | 2007-12-20 | 2009-06-25 | Canon Kabushiki Kaisha | METHOD FOR PRODUCING ELECTRODE HAVING IMMOBILIZED pi-CONJUGATED LIGANDS, ELECTRODE HAVING IMMOBILIZED pi-CONJUGATED METAL COMPLEXES, AND ELECTRODE HAVING IMMOBILIZED pi-CONJUGATED MULTINUCLEAR METAL COMPLEXES |
| US8241709B2 (en) * | 2007-12-20 | 2012-08-14 | Canon Kabushiki Kaisha | Method for producing electrode having immobilized π-conjugated ligands, electrode having immobilized π-conjugated metal complexes, and electrode having immobilized π-conjugated multinuclear metal complexes |
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