JPS5850302Y2 - urine test device - Google Patents

Description

[Detailed explanation of the invention] Conventionally performed urine tests are based on five or three items: protein, sugar, pH, urobilinogen, and occult blood.These tests involve observing cloudiness or coloration when a reagent is added. Or dip a test paper in it and observe the color development.

In particular, in the case of color tests, the density of the color varies depending on the density of the urine, and the distribution of components becomes uneven due to chromatography when the test paper is impregnated, so the test results based on color cannot be interpreted as such. There are individual differences in judgment due to the subjectivity of the person in charge of the test, the test is inefficient and is not suitable for processing a large number of samples in mass screenings, etc., and there is a drawback that it is easy to mix up samples and test items when recording test data. there were.

The purpose of this invention is to automate the sending of specimens and testing, thereby eliminating individual differences in judgment of test results, increasing testing efficiency, and eliminating confusion during recording.

In this design, a test strip impregnated with a color reaction reagent is used, and the test strip is supported on a waterproof tape with perforations for continuous processing.

The tape is subjected to a drop of test urine at one location during its travel, and the color reaction is detected optically at another location.

The test piece has a small area of no more than 10 mm on one side.

This is to prevent uneven coloration from occurring due to chromatographic phenomena when the test urine is dropped onto the test strip.

The amount of test urine supplied onto the test piece can be controlled to a nearly constant amount by specifying the number of drops, and one drop is about 0.
．． It is 6 cc.

Further, when optically detecting coloration, white illumination light is irradiated onto the test piece from an oblique direction, and scattered light emitted from the test piece in a substantially perpendicular direction is used for coloration detection.

This is because the surface reflected waves emitted from the test piece in an oblique direction are white light, which impedes the detection of coloration, so we try to avoid receiving these surface reflected waves as much as possible and focus exclusively on the expression of the test piece. and by attempting to receive internally scattered light.

The appropriate illumination angle for the test piece is 40 degrees.

Then, the scattered light of the test piece is photometered at different wavelengths, the ratio of both photometry results is calculated, and the inspection result is determined based on the ratio.

By dividing the photometric results of different wavelengths in this way, testing can be performed without being affected by fluctuations in power supply voltage, deterioration of the light source, changes in color density due to the darkness of urine, etc.
In the case of a color reaction that causes a hue change, the photometric value of one wavelength can be increased while the photometric value of the other wavelength can be decreased at the same time, making it possible to obtain extremely high sensitivity.

Furthermore, in two-wavelength photometry, the scattered light of a test piece obtained along the same optical axis is divided into two parts by a semi-transparent reflecting mirror, and each part is photometered at a different wavelength.

This is because if photometry is performed at different wavelengths on different positions on the test piece or when photometry is performed at each wavelength on scattered light directed at different angles, errors will occur in the test results.

In addition, according to two-wavelength photometry using a translucent reflector as described above, in order to prevent the above-mentioned errors, the light that is irradiated onto the specimen and scattered along the same optical axis is replaced by a mechanically moving part. Since the process can be performed without using a , the structure of the device is simple and there are fewer failures, and since both photometric results are obtained at the same time, the circuit for calculating the ratio is also simple.

That is, if a test piece is alternately illuminated with light of different wavelengths along the same optical axis, the light from two light sources can be alternately guided onto the illumination optical axis by a chopper, or the light from one light source can be High-speed mechanical movement is required to alternately move the two filters on the road, complicating the structure and mechanical adjustment, and making mechanical wear and failure more likely.

Moreover, since both photometric outputs appear alternately, the ratio of both photometric outputs cannot be calculated unless they are stored using a memory circuit on the side, which increases the complexity of the electrical circuit. .

Next, this invention will be explained with reference to illustrated embodiments.

1 is a tape made of water-resistant material with the same dimensions as 8mrrt movie film, and has feed holes 2, 2...
・, and the length of the two pieces is 3, 3...
are pasted at intervals of four frames.

These test pieces 3, 3... are filter papers impregnated with reagents and dried, and their dimensions are small, 10 mm x 5 myt.

The above-mentioned tape 1 is intermittently pulled out from the reel 4 on which it is wound by a wheel 6 having claws 5, 5 that engage with the feed holes 2, 2, . . . .

Test pieces 3, 3, etc. are sent in order with this tape 1.
. . . first receives urine dripping under a suitable dripping device 7 and then enters the tunnel 8.

After being sent into the tunnel for about 30 seconds, inspection window 9
reach the position.

The test piece 3 in the inspection window 9 is illuminated from approximately 400 oblique directions by a light source 10, and the scattered light 11 that exits in a direction perpendicular to the surface of the test piece passes through a condenser lens 12 and a filter 13. Enter the semi-transparent mirror 14, where 11a and 1
1b, and filters 15a and 15, respectively.
b and enters the light receivers 16a and 16b.

The respective outputs of the photodetectors 16a and 16b are amplified by logarithmic amplifiers 17a and 17b, respectively, and then both enter a differential amplifier 18.

This difference output is digitized by the AD converter 19 and supplied to the computer 21 via the line 20a.

Multiple sets of inspection machines similar to the above I to 19 are prepared separately,
These are test pieces 3, 3 and 3, each impregnated with a different type of reagent.
... is used, and the wheels 6 of each inspection machine are operated synchronously.

The digitized inspection output of each inspection machine is
b, 20c... and is supplied to the verification machine 21.

The inspection results from each inspection machine are sent to the line printer 22.
are supplied to the printer and printed on the same line all at once.

In the above-mentioned apparatus, urine of a patient at a certain time is simultaneously dropped onto the test pieces 3 of a plurality of tapes 1.

The test piece 3 enters the tunnel 8 due to the movement of the tape 1, and after a sufficient time has elapsed for the reagent contained in the test piece to react with the urine, they reach the inspection window 9 all at once.

The tunnel prevents the specimen from drying out during this time and prevents the color from fading due to external light.

The filter 13 selectively passes only the wavelength band necessary for the inspection item from among the scattered light of the test piece 3, and removes light rays of unnecessary wavelengths.

The filters 15a and 15b each pass two wavelength bands related to the inspection item.

The ratio of the output of the light receiving element in these two wavelength bands appears on the output side of the differential amplifier 18, and the calculator 21 uses this as a reference.
The test results will be judged within.

Data on multiple types of test items for the same patient are printed on the same line by a line printer.

In the above example, the test piece 3 is small, with no more than 10 peaks on one side, as is clear from FIG. seep through.

When inspecting coloration, since the light is illuminated from an oblique direction and the scattered light is captured from a right angle, there will be no damage caused by light reflected from the surface of the test piece.

In addition, when photometrically measuring two color components using a filter, the scattered light traveling in the same direction is divided by a semi-transparent reflector, which eliminates inspection errors caused by differences in the inspection location and inspection direction of the specimen. can be avoided.

Since the test result is obtained as a quotient of the intensities of two reflected lights of different wavelengths, it is not affected by changes in color density due to differences in urine concentration.

As described above, in this invention, test pieces are used instead of conventionally used reagents and test strips, and they are attached to tapes with feed holes at regular intervals, and each tape can be operated synchronously. Moreover, there is no need to make mistakes in sample numbers or test items, and furthermore, through mechanization, it is possible to eliminate individual differences in judgment of test results and improve test efficiency.

Incidentally, instead of operating a plurality of tapes synchronously as in the above embodiment, a single tape with a plurality of test pieces arranged successively or side by side may be used.

[Brief explanation of drawings]

FIG. 1 is a diagram showing an example of a test tape used in this invention, and FIG. 2 is a block diagram of one embodiment of this invention. 1...Test tape, 2...Spread hole, 3
......Test piece, 5...Feeding claw, γ...
...Urine warming device, 10...Light source, 14...
...Semi-transparent mirror, 15a and 15b...Filter, 18...Differential amplifier.

Claims (1)

[Scope of utility model registration request] A test tape in which test pieces each impregnated with a reagent not exceeding 1 CtfL are attached at regular intervals to a water-resistant tape having a feed hole, a tape feeding mechanism having a feed claw that engages with the feed hole, and the tape. A device that drips urine onto the upper test piece, a light source that irradiates light from an oblique direction onto the test piece onto which urine has been dropped, and a translucent reflector that divides the scattered light emitted from the test piece in an approximately perpendicular direction into two. , filters with different transmission wavelengths interposed in each path of the divided scattered light, elements that receive the light transmitted through each filter, and the product of electrical output of each element. A urine testing device consisting of a division circuit that calculates .