JPS58216960A - Rinsing unit in blood inspecting apparatus - Google Patents

Abstract

PURPOSE:To carry out the rinsing of a specimen accurately and smoothly, by a delivering needle and a recovery tank are connected for a definite time after a rinsing liquid is delivered to extract the rinsing liquid in the delivering needle. CONSTITUTION:Plural pairs of delivering needles 1 and suction needles 2 are assembled with the under surface side of a head A and the needles 1 are connected to a rinsing liquid tank 13 through a rinsing liquid supply pipe 12 while the needles 2 are connected to a recovery tank 22 through a suction pipe 21. In rinsing, a test tube (a) is raised by operating a lift apparatus (not shown by the drawing) and a valve 17 is opened in such a state that a pressure pump 15 is operated to inject a difinite amount of the rinsing liquid. In the next step, the residual rinsing liquid is recovered through a bypass pipe 5 by using a vaccum pump and each needle 2 is brought to a state reaching the bottom of the test tube (a). In this state, the rinsing liquid in the test tube (a) is recovered and these processes are repeated.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a blood test that is used in the medical field to test a patient's blood by washing a test tube containing reaction beads (beads) reacted with blood as a specimen. This invention relates to improvements to the cleaning device in the device.

As shown in Figure 1, blood tests of patients are usually carried out in medical practice in a large number of test tubes (a) supported in parallel on a rack (3) (or in test holes arranged in a large number on a titration plate). ), titrate a predetermined amount of blood as a sample,
React with the reagent on the surface of the reaction sphere b (beads coated with a reagent that reacts with blood) that has been placed in the test tube a in advance (the reagent is applied to the inner wall of the test tube or the inner wall of the test hole). Next, the reaction bulb in test tube a is washed with a cleaning solution and the surface of the washed reaction bulb is inspected. The reaction bulb b (or the inner surface of the test tube or test hole) in the test tube or test hole is usually cleaned using a rack (3) that supports the test tubes a in parallel.
(or a titration plate with a large number of aligned test holes) is set on the base plate (4) provided in the machine frame K of the washing device M, as shown in FIG. Each test tube a... is placed in a state where it faces a large number of dispensing needles (1) and suction needles (2), which are arranged vertically on two heads A of the base plate (4), From this state, the cleaning liquid is fed to the head A, and a certain amount of cleaning liquid is injected into each test tube a from each dispensing needle (2). Then, the base plate (4) is raised and lowered. N (4
0) (in some cases, head A may be lowered) and the suction needle (2) enters each test tube a, and the operation of the vacuum pump connected to head A causes each suction ff1l (2) to enter each test tube a. ) to remove the cleaning solution by suction, then lower the platform (4) and pull out the suction needle (2) upwards from inside the test tube a. 1) Dispense by... and repeat this process.

The injection of the washing liquid from each dispensing needle (1) is controlled by injecting the washing liquid into the lumen of the head A on the rear end side of the lumen (as shown in Fig. 3). The distribution trunk (10a) that continues long in the left and right direction on the upper end side) and its distribution trunk (]O
A distributor (10) consisting of a large number of longitudinal distribution branches (]Ob) whose base ends are connected to a) and arranged in a comb-teeth shape.
and each distribution branch (10b) of the distributor (10)
The base end of each dispensing needle (1) is connected to the distributor (10), as shown in FIG.
A cleaning liquid supply pipe (12) communicating with a cleaning liquid tank (13) whose inside is pressurized via a pressurizing pipe (14) by a pressure pump (15) is connected, and this cleaning liquid supply pipe ( A valve V is provided in the middle of 12), and the operation is controlled by the valve V.

By the way, in this conventional means, when the cleaning liquid supply pipe 02 leading to the distributor (10) is shut off by the operation of the valve (1) and the dispensing of the cleaning liquid is stopped, over time, The cleaning liquid drips from the tip of the dispensing needle (1), and due to the dripping, the cleaning liquid in one of the many dispensing needles (1)... When the lumen of the dispensing needle flJ becomes empty, the inside of the distributor (10) communicates with the outside through the lumen of the dispensing needle flJ, which has become empty, and the dispenser (10) is broken and air enters into the distributor (10) from the empty lumen of the dispensing needle (1).
)... This causes an inconvenience in that the cleaning liquid accumulated in the distributor (IQl) flows down from the distributor (IQl).

The present invention was made to solve the problems of the conventional means, and an object of the present invention is to provide a novel washing device that can accurately and smoothly wash a specimen.

The invention will now be described with reference to illustrative embodiments. Note that the same reference numerals are used for parts common to the conventional device.

In Fig. 2, A is a head, and on its lower surface, multiple pairs of dispensing needles (1) and suction needles (2) combined to form pairs are assembled at predetermined intervals. There is.

Below the head A are a number of test tubes a-a... or
Base plate (4) for setting the titration plate with many holes.
is mounted so that it can be raised and lowered via a lifting device (40). The elevating device (40) is used to relatively change the distance between the head 8 and the base plate (4), and may be used to raise and lower the spatula 1'A.

Inside the head A, as shown in Fig. 3, there is a distribution main passage (]Oa) located at the rear end side (upper end side in Fig. 3) of the head A lumen and continuous in the left-right direction. , its distribution trunk (]
A large number of distribution branches (]Ob) in the anteroposterior direction (downward to − in FIG. 3) arranged in a comb-like shape with their base ends connected to Oa).
...and the distributor (10
) is installed, and its distribution branch (]Ob)...
Each of the aforementioned dispensing needles f attached to the bottom surface of the head A
The proximal end (]a) of il... is the connection point. Also,
Inside the head A, there is a liquid collection main path (20a) arranged at the front end side of the head A lumen and continuous in the left and right direction, and a liquid collection main path (20a) whose proximal end is connected to the liquid collection main path (20a). A liquid collector (20) is made up of a large number of liquid collection branches (20b) arranged in a comb-like shape in the front and back direction, and each liquid collection branch (20b) is connected to the distributor (10). Aligned distribution branches (10b)...
The above-mentioned suction needles (2)... have their proximal ends located at the bottom of the liquid collection branch (20b) of the liquid collector (20). (2a) is the connection. The liquid collector (20) is connected to its liquid collection trunk (20a).
The connected pipe (20C) is made to protrude from the top side of the head A, one end of the suction pipe (21) is connected thereto, and the other end of the suction pipe (21) is connected as shown in FIG. As shown, it is connected to a recovery tank (22), and a vacuum pump (24) is connected to the recovery tank (22) via a communication pipe (23).
) to the lumen of the suction needle (2).

Also, the distributor 00) has its distribution trunk (]
A connecting pipe (IOC) to be connected to Oa) is made to protrude from the upper surface of head A, and one end of the cleaning liquid supply pipe 02) is connected thereto, and the other end of the cleaning liquid supply pipe (Oa) is connected to the cleaning liquid tank. (13) is connected and communicated with the cleaning liquid tank (13).
) is connected to a pressure pump (15) via a communication pipe (14).
are connected and communicated with each other, and the cleaning liquid is supplied to the distributor (1) by pressurizing the air 1F in the cleaning liquid tank (13) by the pump (I5).
0) and then to each dispensing needle (1).

The cleaning liquid supply pipe (12) is provided with a first valve (17) in the middle thereof. The valve (1
7) is opened for a certain period of time and supplies a certain amount of the cleaning liquid in the tank (13) to the distributor (10).

Further, the first valve (17) of the cleaning liquid supply pipe 02)
) and the distributor (10),
As shown in FIG. 4, a bypass pipe (5) communicating with the above-mentioned suction pipe (2) is connected.The bypass pipe (5) is provided with a second valve (51).

The second valve (51) is normally closed,
When the first valve (17) performs an opening operation and closes, it is opened for a certain period of time to apply suction pressure to the distributor (10). b is the piece in test tube a.

Next, the main functions of the cleaning device configured as described above will be explained.

First, a rack (3) in which test tubes a-a... are supported in parallel is set on a platform (4), and the distance between it and the dispensing head A is adjusted appropriately by operating the lifting device (4). Dispensing needle (1) and suction needle (2) combined in a pair above each test tube a
The situation is such that

Subsequently, while the pressurizing pump (15) is in operation, the first valve (17) is operated at a certain time. Then, the cleaning liquid in the cleaning liquid tank (13) is transferred from the cleaning liquid supply pipe (I2) in a pre-calculated amount determined by the opening time of the first valve (17) to the first valve 07). and is fed to the distributor (10) side. Then, the distributor (10) distributes the cleaning liquid to each of the wave nails (1), (1), etc., and a predetermined amount of cleaning liquid is applied to each test tube a-a from each dispensing needle (1).
...is injected into the body.

Next, by operating the elevating device (40), the relative distance between the base plate (4) and the head A is reduced, and the lower end of each suction needle (2) provided on the dispensing head A is Test tube i... Let it reach the bottom of the tube.

As a result, each suction needle (2)... is connected to the vacuum pump (24).
) (the vacuum pump (24) is always in operation), the pressure in the recovery tank (22), which is at negative pressure, sucks out the cleaning liquid in each test tube a. The collected waste is collected into the collection tank (22) through the pipe (21).

Next, operate the lifting device again and place the suction needle (2)...
is pulled upward from each test tube a...
Dispense the washing liquid from the dispensing needle (1) into each test tube a using the operations described above, and then transfer the washing liquid to the suction needle (2)...
・Suction and collect, and repeat this as many times as necessary.

This washes the inner surface of each test tube a and the reaction bulb, but the device of the present invention further allows the washing liquid to be applied to each dispensing needle (1) and the reaction bulb.
When dispensing from ... into each test tube a is completed, it acts as follows.

That is, by opening the first balloon (17) for a certain period of time, a calculated amount of washing liquid is dispensed into each test tube a, and when the first valve (17) is closed, each dispensing needle (1)・(1
] ... stops, but in conjunction with the closing operation of the first valve (17), the second valve (!51) stops.
) is opened for a certain period of time, and the bypass pipe (5) is connected to the pipe (12), the distributor OQl, the dispensing pipe (Ill (11)..., the dispensing needle (1), flJ... Suction pressure from the vacuum pump (24) acts through the
Each dispensing needle (1) ... the cleaning liquid that is filled in the cleaning liquid supply path between the inside and the first valve (1η) and remains is transferred to the recovery tank t22 by a predetermined amount. Each dispensing needle (1) acts to collect and draw out the washing liquid from inside. The reverse recovery amount of the washing liquid is determined by changing the liquid level of the washing liquid remaining in the washing liquid supply path to the lower end position of each dispensing needle (1) supported by the dispensing head A. This is to completely prevent the washing liquid from dripping from each dispensing needle (1) by returning it lower than the second valve (5υ
Set according to the opening time.

As explained above, the washing device in the blood test device according to the present invention is capable of dispensing a calculated fixed amount of washing liquid into each test tube (or test hole), and after dispensing the washing liquid into each test tube. When the pressure that was acting on the injection needles stops, each dispensing needle is connected to the recovery tank under negative pressure for a certain period of time, and the cleaning liquid in each dispensing needle is drawn out. This eliminates the problem of liquid dripping from the dispensing needle when dispensing an amount of washing solution, causing uneven washing, and the washing of each test tube and reaction bulb is now easier. Be able to do it accurately.

[Brief explanation of the drawing]

FIG. 1 is a perspective view of a rack and test tubes, FIG. 2 is a partially cutaway side view of the apparatus according to the present invention, FIG. 3 is a cross-sectional plan view of the head of the above apparatus, and FIG. Explanatory diagram, 5th
The figure is an explanatory diagram of a conventional device. Explanation of drawing symbols A...Head 1...Dispensing needle 1a...
Base end portion 10...Distributor 10a...Distribution main path 10b...Distribution branch path 12...Washing liquid supply pipe 13...Washing liquid tank 14...Communication pipe 15...Pressurization Pump 17...first valve 2
...Suction needle 2a...Proximal end 20...Liquid collector
20a...Liquid collection trunk 2b...Liquid collection branch 2]...
- Suction pipe 22... Recovery tank 23... Communication pipe 24... Vacuum pump 3... Titration plate a... Test tube b... Beads 4... Base plate 4o... Lifting Device 5...Bypass pipe 5]... Second valve procedure amendment (discharge) Director of the Patent Office Kazuo Wakasugi 1, Indication of the case 1982 Patent Application No. 100066 2, Title of the invention Blood test device Relationship between the cleaning device 3 and the person making the amendment case Applicant name Yasunobu Tsukioka 4, agent address 6-7-n Nishi-Shinjuku, Shinjuku-ku, Tokyo 160 (Stoke Pilding 10F) Name ( 6505)! Buried soil Kazu Shinseki
Department Phone: Tokyo (342) 0826 5, Date of amendment order 6, Number of inventions increased by amendment 7, Subject of amendment ``Description'' 8, Contents of amendment Amend the specification in an attached document. (1), Specification, page 4, line 2, "dispensing needle (2)"
The text has been corrected to read "dispensing needle (1)". (2) In the specification, page 5, line 7, "pressurizing pipe 0
(A)" has been corrected to read "Communication pipe (14) Jl.
)" has been corrected to read "elevating device (40)." (4), Specification, page 16, line 3, l'-2b...
・The phrase "liquid collection branch" has been corrected to "20b...liquid collection branch."

Claims (1)

[Claims] A head on which a dispensing needle and an aspiration needle are attached, a base plate on which a test tube is set below the head, an elevating device that relatively changes the distance between these heads and the base plate, and a vacuum pump for moving the aspiration needle. a suction pipe and a recovery tank that communicate with each other, a distributor that is connected to each of the dispensing needles and is incorporated in the head to distribute cleaning liquid thereto, and a distributor and a cleaning liquid supply pipe connected to each of the dispensing needles. A communicating cleaning liquid tank, a pressurizing pump that pressurizes the inside of the cleaning liquid tank, a first valve provided on the cleaning liquid supply pipe, and an intermediate portion of the cleaning liquid supply pipe between the first valve and the distributor. A bypass pipe is connected to the suction pipe, and a second valve is provided on the bypass pipe, and the second valve is normally closed and opens for a certain period of time when the first valve opens and closes. A cleaning device for a blood test device, characterized in that it is configured to perform cleaning.