JPS58149699A - Method and tool for detection of pulmonary cancer cell and pulmonary microorganism - Google Patents

Abstract

PURPOSE:To carry out the detection of pulmonary cancer cell and pulmonary microorganisms, surely, by collecting the phlegm of the suspected patient day after day, storing the specimen separately by the day of collection, and inspecting the specimen. CONSTITUTION:The vessel 1 for the diagnosis of pulmonary cancer is composed of a nearly cuboidal transparent material and has plural cylindrical cavities separated from each other. The vessel is furnished with the parts for pasting number plates and labels. Each lids 11c-13c is composed of a screw part and a knob. The vessel 2 for the collection of the phlegm for detecting the pulmonary microorganism has the same structure, and can be combined with the vessel 1. The collected phlegm is put into the preservation liquid in the collection part corresponding to the data of the collection and closed with a lid, and the vessel 1 and the vessel 2 are sent to the section for the detection of pulmonary cancer cell and the section for the detection of microorganisms, respectively. A necessary number of the vessels each having a lid may be combined together and used in combination with a container bag.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for simultaneously detecting lung cancer cells and organisms in the lungs such as Mycobacterium tuberculosis through a cell test using sputum, and an apparatus used therefor.

□In recent years, the number of cancer cases has increased the most in the world, and the rate of increase in lung cancer in particular is about 160 times that of other cancers.In Japan, the mortality rate from cancer, especially lung cancer, is rapidly increasing. This accounts for more than 26% of all cancer deaths worldwide.

Among these cancers with high mortality rates, if we consider the trends in mortality rates for lung cancer, pulmonary tuberculosis, and gastric cancer over a 40-year period from 1950 to 1988, we find that the mortality rates for pulmonary tuberculosis and gastric cancer have decreased, as shown in Figure 1. Regardless of the situation, lung cancer rates were approximately 16 times higher in 1980 than in 1950, and
It is estimated that 64# is more than 26 times as large, which is why we are hoping for the emergence of a lung cancer test C11L, a testing method that is simple and highly accurate for early detection.

The causes of this rapid increase in lung cancer are said to be air pollution, smoking, chemicals, an increase in the elderly population, and a decrease in deaths from other diseases due to the development of various treatments.As is well known, lung cancer occurs in the hilar region. There are two types of cancer: the hilar type and the field type, which occurs in the pulmonary bulge.The former type of cancer is extremely closely related to smoking, and includes squamous cell carcinoma and small cell undifferentiated carcinoma, which is the most common type of lung cancer. Of these, the latter cancer accounts for approximately 66%! Although the impact caused by smoke is said to be less severe than the former, it is said that this applies to adenocarcinoma and large cell undifferentiated cancer, accounting for approximately 45% of cases.

Cancer that develops in the hilar region (also called central type) cannot be diagnosed, especially in the early stages, because it overlaps with the central shadow on chest X-ray photographs due to the structure of the human body, and dark tear examination is essential.

Therefore, in the past, for inpatients, the main cells were collected and cell tests were performed at the same time, and for outpatients or patients located far from the testing facility, an 8-day supply was collected via a preservation solution in a bottle or sputum bag. Sequentially collect the amount in a container such as a bottle,
In the case of a katsu bottle, the mixture is stirred, and in the case of a bag, it is taken out from the mixture and inspected.As a result of the experiment, 1
The detection rate is approximately 80% with a single sputum test, and even when 8 days' worth of samples are mixed in the same container, there are differences in the order of collection, destruction of cells, and the part extracted into the specimen. , the detection rate is almost similar to that of sputum examination performed only once in #E,
Accurate testing is not possible.

Furthermore, lung cancer examinations are performed using chest X-ray photographs, but as mentioned above, hilar cancer occurs in the large bronchi and overlaps with the central shadow due to the structure of the human body, so it is difficult to detect hilar cancer, especially in the early stages. However, since lung field cancer occurs in small areas of the bronchi, it is relatively easy to detect abnormalities in X-rays, but early small shadows can be diagnosed as lung cancer. It is difficult to diagnose whether it is just inflammation, or whether it is pulmonary tuberculosis or a chest disease such as true 1 syndrome, and even now it is not possible to clearly distinguish between these at this stage. The current situation is that there are particularly few medical practitioners.

1. Therefore, by performing not only the lung cancer 1ll1i drop test, which is relatively easy to judge clearly at an early stage, but also the general bacteriological test, the mortality rate for lung cancer tests and especially for chest diseases can be reduced by 9%. However, the decrease in ÷ means that the number of new patients has not disappeared and the disease is still serious, such as pulmonary tuberculosis, which is said to be difficult to develop in the same person as lung cancer. There is a strong demand for an inspection method and equipment to be used for it.

child? ,? In view of J, the present invention allows lung cancer cell testing to be performed every day for an arbitrary period of time in the dark from the human body, and
At the same time, a series of dark tears for testing microorganisms in the lungs, such as pulmonary tuberculosis bacteria, are collected in sequence into a set of identified container-shaped ## tools, and by testing each of these dark tears, lung cancer can be detected. A series of tests are conducted in conjunction with tests for cells and microorganisms in the lungs,
This was done with the purpose of providing a highly accurate test for lung cancer cells, as well as a method for testing microorganisms in the lungs, as well as equipment for conducting this test efficiently. is 1. 1) Dark tears are secreted from the human body every day for any period of time, only the darkness on the last day of the dark tear period is taken as the main character, and the others are separated and collected every day through a preservation solution, and a series of sputum specimens are collected through the preservation solution. A method of conducting a lung cancer test and also conducting a lung microorganism test based on the main character on the last day. A sputum collection container for lung cancer testing that is distinguishable, has the shape of a sealed container, and is formed to be able to contain a preservation solution; The present invention is a device formed by forming a sputum collection container part for intrapulmonary biological examination into a combination shape.

Note that the preservation solution herein is a general term for a solution that is made of, for example, methanol, thymol, and saline, and is used to prevent dark sputum cells from rotting and to prevent cell degeneration.

Hereinafter, in order to further clarify the gist of the present invention described above,
An embodiment of the present invention will be described in detail with reference to the drawings as follows.

Embodiment 1 of the present invention is shown in FIGS. 1 to 142, and numeral 1 in the figures is a rectangular parallelepiped sputum collection container for lung cancer testing made of a transparent material, and is placed upward at the position of wheel a. In the sampling section 11, which is open and divided into a cylindrical wl of arbitrary depth, 1
1, 18k is drilled.

Female screws are screwed into the mouths of the collection parts 11h, 12h, and 18h, and numbers "11" and "2" are carved from right to left on the upper wall of the container l near the mouths. 8"
The identification symbols ita, 12hata, 18& are displayed,
Further, on the front cki wall of the container section 1, each of the sampling sections 11
Identification labels 11b%ttb, isb are attached to the front positions of h, 12h, and 18h, displaying the name of the person to be examined, the date and time of darkness, and the medical facility/ward (department, ridge), etc., and are attached to the left side wall. In the section, four sections 81 forming upwardly open dovetail-shaped engagement sections 8 are formed in nine stages in the vertical direction from the top wall.

11 (111! 0118e is a bud, and each collection part 11h, 12 of the former container part l has a male screw part formed to be screwed into the threaded part carved on the mouth of 18, and a male screw part near the center. It is integrally formed with seven flange parts each having a vertical wall-shaped handle part 1110.1!1Cs181c inside, and the handle part 1
11g, 1210% On the top of 181e is the music identification number 1
1&, 12a.

Identification symbol same as 18 &11' Hatake, 12'a % 18
'a' is displayed respectively.

In addition, when the above-mentioned Sugarie, 11, 18 (+ male screw part and the sampling part 11h, 12h, 118h female thread part of the mouth part of the container part 1 are screwed together, the collection part 11h, tgh, 18
k is designed to become a sealed chamber as appropriate (via a gasket, etc. if necessary).

Next, reference numeral 2 denotes a sputum collection container for testing pulmonary organisms such as pulmonary tuberculosis 1, which has the same rectangular parallelepiped shape in longitudinal section as the container 1 and is made of a transparent material, and can be opened upward at an appropriate position. A sampling section 121h partitioned into a cylindrical chamber of any size is drilled, a female screw is engraved in the mouth part t of the sampling part 21b, and an identification mark Jij21a is displayed on the mouth part 21b. In addition, there is a book label f on the side of the container.
Identification label/I formed identical to lb-12b, 111
/21b is attached, and the recess 81 is attached to the right side wall.
A convex portion 82 is formed to protrude vertically from the upper wall for fitting.

21 (l is a bud, and the above *1xcs12e.

Similar to 18C, a male screw part is provided, and a flange part is integrally provided with a handle part 211C installed in the shape of a standing wall at the upper part, and the same identification symbol 21a as the identification symbol 21 Hatake is displayed on the handle part 211C. Even if it is already mentioned above! 21 (+) is screwed into the collection part 21k, the collection part 21k is formed as a closed chamber, similar to the container part 1.

Next 1' (-% ■ is the preservation solution, an appropriate amount of methanol,
It is a mixture of thymol and saline, and an arbitrary amount of this is collected in the collection portions 11h, 1! ! h.

The liquid was injected into each tube at 18.

In this way, the device in the first embodiment is constructed by combining the container part 1 and the container part 2, etc., and when using this, first, on the 1st day of collection ts, when waking up early in the morning, Rinse and clean the inside of the subject's mouth, then take a deep breath, cough loudly, and gasp, and expectorate the phlegm that is secreted from deep into the lungs into a clean, dry volume.

If you find it difficult to extract 4, it will be easier if you open your mouth wide and repeatedly inhale the steam from the boiling water, then cough it out.

Pick up the sputum that has been coughed up in this way with a chopstick, etc., and remove it from container part 1.
After opening the lid of the bud and confirming that the storage solution is in the collection part 11h, put the file into the collection part 11h, hold the handle part 111c of the file, and insert it into the threaded part of the collection part 11h. sure 5
N! Screw it together to prevent it from getting wet in the storage solution.

Then, in the 2nd sampling cylinder and the 88th sampling cylinder, f
On the 1st and 1st day of JtI period collection, the test subject coughed up the first hard tube in the front tube.
Day and day are collected through preservation solution K into Jl [section 18h,
Securely screw the lid 12 (1,180) onto the mouth of the sampling section 12b%18h.

Furthermore, in the 48th collection, in the same manner as described above, only the main material is aspirated into the collection part 21h of the container 2 with the lid 21e opened, and then the lid 21 (I is put into the mouth of the collection part 21h). Securely screw together.

The containers ff1 and 2 containing the 4 sputum collected in this manner are combined into one body and immediately delivered to the testing facility.

In this testing facility, the container part 1 is separated from the container part 1 by lifting the container part 2 upward to separate the convex part 82 from the recess part 81 of the container part l. During the examination, in the lung cancer examination department where the cytology instructor conducts the examination, for example, out of the dark lachrymal components collected each day, the parts that are likely to contain cancer cells are selected and removed, thereby obtaining a clean tissue. Prepare specimens for each person, screen them to avoid oversights, and
The presence or absence of cancer cells is examined using a sputum microscopy.Meanwhile, the container 2 separated from the container 1 is sent to the lung microscopy examination department, where it is subjected to a microscopy using a smear as described above. to check for the presence of tuberculosis 1 class organisms in the lungs.

In this way, even though samples are collected for 4 consecutive days, tjs 1st ~
@Until the 8th, samples were collected using preservation solution K.
There is no putrefaction or denaturation of the sputum, and the sputum used for lung biological testing is kept in a test bag all day long, and immediately delivered to the testing department and tested separately, so there is no need to use a preservation solution. In both cases, highly accurate tests can be performed by performing a series of tests on sputum without spoiling or denaturing it.

Next N: Figures a to 6 show a second embodiment of the present invention, in which 4 is a sputum collection container for lung cancer testing, which is different from the above-mentioned embodiment. A container body 41 for collecting on the first day and a container main body 4 for collecting on the second day! and the container body 48 for collection on the 8th day are separated and made of transparent material.
It is formed into a cylindrical shape with a bottom, and the inside is a collection part 41.
b, 42, and 48h, and the identification symbols 411i, 42hatake, and 48& of the number game 1:2.8 are displayed in sequence on the upper part of the outer surface, and below these, the above-mentioned identification lape/
Identification rahe yv4 with the same content as L'llb, 12b, 18b
lb, 42b, and 48b are attached to each other.

Saratko, 41e, 42c, 4Jl are the quantities, and the identification numbers 418, 42&, 48& and the same numbers as the identification symbols 418, 42&, 48& are displayed on the top surface of the disks, and the identification numbers 41', 42'a, and 48 Lu are displayed. Each of the container bodies 41, 42, 4 is made up of a shaped flange portion, and a cylindrical fitting portion extending integrally from the flange portion and having a ring-shaped protruding portion around the outer circumferential position as appropriate.
Fits into the mouth of No.8.

, the sampling portions 41h, 42h, and 48h are formed as close as possible. In addition, the sampling portions 41h, 42h, 48
An arbitrary amount of the above-mentioned preservation solution is injected into each part of the tube.

Next <6 is lung 4ii! This is a sputum collection container part for testing of living organisms in the lungs such as F bacteria, and is made up of a container body 51 having the same shape as the container body 41, 42.4g, and housing a collection part 51h inside.
An identification symbol 61& of the number 4 is displayed on the upper part of the outer surface of this, and an identification label A/61b having the same content as the identification label A/21b is pasted below it.

Further, 61C is a lid with the identification mark 51 on the top surface.
An identification symbol 51&, which is the same as &, is displayed, and the container body 6
It is formed so that it can be fitted into the mouth of the sampling section 51h of No. 1.

410, 420, and 480 are individual storage bags, one side is open with a transparent film such as vinyl, and a strip-shaped fitting part is attached to the tip of the open opening. There are 8 of them in a bag shape, @t
In the container body 41, 42, 48 containing the J period preservation solution K, f
i41(1,426,48(+) is attached.In this way, the storage bag 410
．． The container body 41, 42, 48, etc. stored in the container body 420.480, and the tale fitted to the mouth of the container body 51 are assembled into a set, and one side is opened with a transparent film such as vinyl. Then, it is stored in a bag-like storage bag 6 having a strip-shaped fitting part around the open end, and a manual 7 containing instructions for use is stored in this bag so as to be visible from the outside. There is.

As described above, the tool a in the second embodiment is configured by combining the container part 4 and the container part 5, etc., and when using this, the storage bag 6 and the storage bags 410, 420.
The container part 4.6 is taken out from the container part 80, and then inspected according to the first embodiment, and then put back into the storage bag as a set, and inspected in each inspection department according to the first embodiment. conduct.

In the above example, the method of collecting sputum for lung cancer test every day for 8 days and testing it was described in detail, but the method is not limited to this, but also includes collection of sputum for 5 days or more. The buds are fitted together with dovetail-like attachment parts to form an integral body, but the buds are not particularly limited to this as long as they can be easily combined or removed in one piece, and the shape of the buds may be such that the collection part can be sealed. It may be formed in any way, and the lid and the collection part do not have to be screwed together, but other methods may be used as long as the lid and sampling part are sealed to prevent leakage of the contents inside.

Furthermore, the content of the Icfa-specific label may be arbitrary, and the identification symbol may be of any other type as long as the collection site can be easily identified.

As described above, the present invention is not limited to these embodiments, and the configuration thereof may be arbitrarily determined within the range in which the above-described objects, functions, and effects of the present invention are achieved.

As explained in detail above, in carrying out lung cancer cell tests, the present invention is designed to carry out a series of tests for living organisms in the lungs, such as pulmonary tuberculosis cocoons, which are easily misdiagnosed due to chest diseases. Finally, on this last day, we collected the main material for the lung microbial test, and the dark tears collected up to the second day before were filtered separately and collected through a preservation solution, so we collected them in series. Dark tears for lung cancer tests do not decay or degenerate at all even if it takes several days to collect them, and dark tears for lung microbial tests can be collected on the final day without using a preservation solution, and can be collected on the last day without using a preservation solution. There is no problem at all since it is inspected (it can be collected effectively).

In addition, as a result of the experiment, the detection rate of cancer cells was only about 80% when the sputum sample was collected from a single day of incisions or samples from several days were mixed in the same container. In the method of collecting sputum every day as in the invention, separating the samples, and testing the sputum each time, the amount of sputum collected is approximately 70 to 80 in 8 days and 80 in 4.5 days.
The detection rate was ~90%, making it possible to perform an extremely accurate test.

Furthermore, the equipment should be divided into sections for convenient collection of sputum for lung cancer cell testing and pulmonary microorganism testing, and the container for lung cancer cell testing should be easily collected on separate days. Since it is designed to be clearly identified, anyone can use it easily.

In addition, the overall structure is compact and well-organized, making it easy to carry and handle.Furthermore, the container part has an engaging part, and the external shape is a stable rectangular or cylindrical shape, so it can be inspected if necessary. It can be used by fitting it into a support stand, and it is extremely easy to handle without causing unexpected problems such as falling over during inspection.

Furthermore, if chest X-ray examination is used in addition to the dark tear test described above, it will greatly contribute to early detection, and the contribution to humanity will be extremely large. I have to say that this is a big deal.

[Brief explanation of drawings]

The drawings show one embodiment of the present invention, and FIG. 1 shows a perspective view of the m-tool according to the present invention, with the lid opened, and FIG. 2 shows the same as shown in FIG. 1. Figure 8 is a central cross-sectional view of the assembled state with the lid closed. FIG. 4 is a plan view showing a partial storage case, and FIG.
The figure is a partially cutaway plan view of the storage bag containing the instruction manual, and Figure 6 is a graph showing trends in male mortality rates per 10,000 people due to lung cancer, pulmonary tuberculosis, and gastric cancer. 41.4 - Sputum collection container for lung cancer testing 2.5 - Thin noodles - Sputum collection container for lung internal biological testing 41.42% 48.
51-Ichishunki body ll approx. 12h% 18h, 21 slope 41 slope 42 slope 48h, 5
th---Collection part 111.12"s 19m, 21"s 41"s
42"s 4B"s 51' ---Kamabetsu era identification label 110% 126% 180% 210% 4C% 42(
1% 48 (1% 5C - Part. ooooo. [F] LO size - [Bello = s < Punishment C ■ 0ri Fig. 3 Fig. 4

Claims (2)

[Claims] (1) The main material excreted from the human body every day for any period of time is separated and collected in a preservation solution, and the weight of the main material is separated from the raw material in the preservation solution and collected, and then the main material is collected in the preservation solution. A test for lung cancer cells and microorganisms in the lungs, characterized in that the presence or absence of lung cancer cells is tested by using the sputum inside each day as a sample, and the detection of microorganisms in the lungs is also performed using only the main character as a specimen. Method. (2) The main parts excreted from the human body every day for an arbitrary period of time are separated using a preservation solution and placed in a sealed container shape #C which is marked with an arbitrary number of X- marks and can be identified. A combination letter includes a sputum collection container for lung cancer testing, and a sputum collection container for lung biological testing, which is arranged in at least one @#r-area, is identifiable, and is shaped like a sealed container. An instrument used to examine lung cancer cells and microorganisms in the lungs, which are characterized by their formation in the lungs.