JPS5774087A - Separation and purification of placental enzyme transformating angiotensin (a tase) - Google Patents
Separation and purification of placental enzyme transformating angiotensin (a tase)Info
- Publication number
- JPS5774087A JPS5774087A JP15123480A JP15123480A JPS5774087A JP S5774087 A JPS5774087 A JP S5774087A JP 15123480 A JP15123480 A JP 15123480A JP 15123480 A JP15123480 A JP 15123480A JP S5774087 A JPS5774087 A JP S5774087A
- Authority
- JP
- Japan
- Prior art keywords
- tase
- supernatant
- transformating
- angiotensin
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000015427 Angiotensins Human genes 0.000 title 1
- 108010064733 Angiotensins Proteins 0.000 title 1
- 102000004190 Enzymes Human genes 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 title 1
- 230000003169 placental effect Effects 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 238000000926 separation method Methods 0.000 title 1
- 239000006228 supernatant Substances 0.000 abstract 3
- 239000007864 aqueous solution Substances 0.000 abstract 2
- 210000002826 placenta Anatomy 0.000 abstract 2
- 150000003751 zinc Chemical class 0.000 abstract 2
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- ZOIORXHNWRGPMV-UHFFFAOYSA-N acetic acid;zinc Chemical compound [Zn].CC(O)=O.CC(O)=O ZOIORXHNWRGPMV-UHFFFAOYSA-N 0.000 abstract 1
- 125000000129 anionic group Chemical group 0.000 abstract 1
- 239000003945 anionic surfactant Substances 0.000 abstract 1
- 150000001450 anions Chemical class 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 238000000354 decomposition reaction Methods 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 238000005194 fractionation Methods 0.000 abstract 1
- 238000002523 gelfiltration Methods 0.000 abstract 1
- 239000002736 nonionic surfactant Substances 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 239000000047 product Substances 0.000 abstract 1
- 238000005185 salting out Methods 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 239000004094 surface-active agent Substances 0.000 abstract 1
- 239000004246 zinc acetate Substances 0.000 abstract 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 abstract 1
- 229960001763 zinc sulfate Drugs 0.000 abstract 1
- 229910000368 zinc sulfate Inorganic materials 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: The extraction with a surfactant, the fractionation of the extract with a zinc salt and other operations are effected to separate and purify the A Tase from tissues of human placenta.
CONSTITUTION: Tissues of human placenta are homogenized together with an aqueous solution containing an anionic or nonionic surfactant and centrifuged into the precipitate and the supernatant. The supernatant is fractionated by salting out with a zinc salt such as zinc acetate or zinc sulfate to recover the resultant supernatant. It is adjusted in pH to 6W8, brought into contact with an anion exchanger equilibrated with a buffer solution of 6W8pH, eluted with an aqueous solution containing 0.5W2M salt and the eluate is treated with a protease for decomposition. Then, the product is subjected to gel filtration to isolate the fraction in which A Tase is active.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15123480A JPS5774087A (en) | 1980-10-27 | 1980-10-27 | Separation and purification of placental enzyme transformating angiotensin (a tase) |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15123480A JPS5774087A (en) | 1980-10-27 | 1980-10-27 | Separation and purification of placental enzyme transformating angiotensin (a tase) |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5774087A true JPS5774087A (en) | 1982-05-10 |
Family
ID=15514174
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15123480A Pending JPS5774087A (en) | 1980-10-27 | 1980-10-27 | Separation and purification of placental enzyme transformating angiotensin (a tase) |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5774087A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59104555A (en) * | 1982-11-08 | 1984-06-16 | アボツト・ラボラトリ−ズ | Method of measuring terminal deoxynucleotidyltransferase |
| US5168061A (en) * | 1986-05-15 | 1992-12-01 | Board Of Regents, The University Of Texas System | Human chorionic peptidase-1 |
| WO1998006829A1 (en) * | 1996-08-12 | 1998-02-19 | Fujisawa Pharmaceutical Co., Ltd. | Methods for preparing bioreactors |
| US6271201B1 (en) | 1993-07-15 | 2001-08-07 | Board Of Regents, The University Of Texas System | Methods for the selective regulation of placental prostanoids and inhibition of labor using IGF-I |
-
1980
- 1980-10-27 JP JP15123480A patent/JPS5774087A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59104555A (en) * | 1982-11-08 | 1984-06-16 | アボツト・ラボラトリ−ズ | Method of measuring terminal deoxynucleotidyltransferase |
| US5168061A (en) * | 1986-05-15 | 1992-12-01 | Board Of Regents, The University Of Texas System | Human chorionic peptidase-1 |
| US6271201B1 (en) | 1993-07-15 | 2001-08-07 | Board Of Regents, The University Of Texas System | Methods for the selective regulation of placental prostanoids and inhibition of labor using IGF-I |
| WO1998006829A1 (en) * | 1996-08-12 | 1998-02-19 | Fujisawa Pharmaceutical Co., Ltd. | Methods for preparing bioreactors |
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