JPS5745458A - Immunologic method for trace analysis - Google Patents
Immunologic method for trace analysisInfo
- Publication number
- JPS5745458A JPS5745458A JP12059780A JP12059780A JPS5745458A JP S5745458 A JPS5745458 A JP S5745458A JP 12059780 A JP12059780 A JP 12059780A JP 12059780 A JP12059780 A JP 12059780A JP S5745458 A JPS5745458 A JP S5745458A
- Authority
- JP
- Japan
- Prior art keywords
- antigen
- antibody
- silver halide
- resulted
- reaction product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title abstract 3
- 230000001900 immune effect Effects 0.000 title 1
- 238000004454 trace mineral analysis Methods 0.000 title 1
- 239000000427 antigen Substances 0.000 abstract 4
- 102000036639 antigens Human genes 0.000 abstract 4
- 108091007433 antigens Proteins 0.000 abstract 4
- -1 silver halide Chemical class 0.000 abstract 4
- 229910052709 silver Inorganic materials 0.000 abstract 3
- 239000004332 silver Substances 0.000 abstract 3
- 239000007795 chemical reaction product Substances 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 2
- 230000001235 sensitizing effect Effects 0.000 abstract 2
- 230000003595 spectral effect Effects 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- OAKJQQAXSVQMHS-UHFFFAOYSA-N hydrazine Substances NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 abstract 1
- 150000002429 hydrazines Chemical class 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 230000003287 optical effect Effects 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/583—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with non-fluorescent dye label
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Silver Salt Photography Or Processing Solution Therefor (AREA)
Abstract
PURPOSE:To increase the sensitivity of detection and to decrease the amount of sample, by bringing a labeled antigen-antibody reaction product into contact with silver halide at the presence of hydrazine compounds. CONSTITUTION:An antigen or an antibody is labeled with a spectral sensitizing dye for photograph as a labeling agent, and a sample contg. the antigen or antibody to be measure, and the antibody or antigen which reacts with the antigen or antibody specifically, are reacted competitively. The resulted antigen-antibody reaction product is brought into contact with silver halide contg. hydrazine compound shown in the general formula (H), and exposed to the light of wavelength which is absorbed by a spectral sensitizing dye. The exposed silver halide is developed, and the optical density of the resulted chromophoric dye is measured. According to this process, the sensibility of detection is enhanced and the multiitem determination can be performed with the quantity of same similar to the usual method.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12059780A JPS5745458A (en) | 1980-09-02 | 1980-09-02 | Immunologic method for trace analysis |
| DE8181106825T DE3170135D1 (en) | 1980-09-02 | 1981-09-01 | Method for immunochemical measurement of trace components |
| EP81106825A EP0047472B1 (en) | 1980-09-02 | 1981-09-01 | Method for immunochemical measurement of trace components |
| US06/298,719 US4404289A (en) | 1980-09-02 | 1981-09-02 | Method for immunochemical measurement of trace components |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12059780A JPS5745458A (en) | 1980-09-02 | 1980-09-02 | Immunologic method for trace analysis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5745458A true JPS5745458A (en) | 1982-03-15 |
| JPS625296B2 JPS625296B2 (en) | 1987-02-04 |
Family
ID=14790190
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12059780A Granted JPS5745458A (en) | 1980-09-02 | 1980-09-02 | Immunologic method for trace analysis |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5745458A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63277971A (en) * | 1987-03-09 | 1988-11-15 | ジヤンセン・フアーマシユーチカ・ナームローゼ・フエンノートシヤツプ | Improving method for precipitating metallic particle onto marker |
| WO2022085762A1 (en) * | 2020-10-22 | 2022-04-28 | 富士フイルム株式会社 | Reagent for measuring skin sensitization, compound, and method for measuring skin sensitization |
-
1980
- 1980-09-02 JP JP12059780A patent/JPS5745458A/en active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63277971A (en) * | 1987-03-09 | 1988-11-15 | ジヤンセン・フアーマシユーチカ・ナームローゼ・フエンノートシヤツプ | Improving method for precipitating metallic particle onto marker |
| WO2022085762A1 (en) * | 2020-10-22 | 2022-04-28 | 富士フイルム株式会社 | Reagent for measuring skin sensitization, compound, and method for measuring skin sensitization |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS625296B2 (en) | 1987-02-04 |
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