JPS5669556A - Protein analyzing chemical and protein quantitative method - Google Patents
Protein analyzing chemical and protein quantitative methodInfo
- Publication number
- JPS5669556A JPS5669556A JP14576879A JP14576879A JPS5669556A JP S5669556 A JPS5669556 A JP S5669556A JP 14576879 A JP14576879 A JP 14576879A JP 14576879 A JP14576879 A JP 14576879A JP S5669556 A JPS5669556 A JP S5669556A
- Authority
- JP
- Japan
- Prior art keywords
- protein
- reagent
- sample
- dye
- quantitative method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
PURPOSE: To provide a protein analyzing reagent and protein quantitative method including dye coomassie briliant blue. G-250, methyl cellurose and acid.
CONSTITUTION: A reagent comprises 2,500.008W0.014% kown dye reagent. briliant blue. G of formula (I), 0.01W0.03% methyl cellurose, and aicd (phosphoric acid, oxalic acid). pH is kept at 0.7W0.9 and if required, a buffering agent for stabilizing pH and a suitable solvent are added in a suitable amount. The colouring degree of the mixing liquid of the chemicals and a sample is measured by a spectrophotometer or a colorimeter to make the protein estimation. In this way, this reagent is not likely to be influenced by a substance coexisiting in the sample and the various type of protein in the sample is estimated with good regeneration and high detecting sensitivety and the mixture solution including the produced protein-dye complex is good in stability, and said reagent is suitable for detecting the protein in such as urine, blood serum, pith liquid, food.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14576879A JPS6049256B2 (en) | 1979-11-09 | 1979-11-09 | Shiyaku for protein analysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14576879A JPS6049256B2 (en) | 1979-11-09 | 1979-11-09 | Shiyaku for protein analysis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5669556A true JPS5669556A (en) | 1981-06-10 |
| JPS6049256B2 JPS6049256B2 (en) | 1985-10-31 |
Family
ID=15392705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14576879A Expired JPS6049256B2 (en) | 1979-11-09 | 1979-11-09 | Shiyaku for protein analysis |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6049256B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5284560A (en) * | 1991-06-24 | 1994-02-08 | Wako Pure Chemical Industries, Ltd. | Acid dye staining method |
| US9091694B2 (en) | 2011-08-19 | 2015-07-28 | Acquascience Limited | Protein detection |
-
1979
- 1979-11-09 JP JP14576879A patent/JPS6049256B2/en not_active Expired
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5284560A (en) * | 1991-06-24 | 1994-02-08 | Wako Pure Chemical Industries, Ltd. | Acid dye staining method |
| US9091694B2 (en) | 2011-08-19 | 2015-07-28 | Acquascience Limited | Protein detection |
| US9377467B2 (en) | 2011-08-19 | 2016-06-28 | Acquascience Limited | Protein detection |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6049256B2 (en) | 1985-10-31 |
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