JPS56151356A - Method and device for measuring of t and b cells - Google Patents
Method and device for measuring of t and b cellsInfo
- Publication number
- JPS56151356A JPS56151356A JP5489880A JP5489880A JPS56151356A JP S56151356 A JPS56151356 A JP S56151356A JP 5489880 A JP5489880 A JP 5489880A JP 5489880 A JP5489880 A JP 5489880A JP S56151356 A JPS56151356 A JP S56151356A
- Authority
- JP
- Japan
- Prior art keywords
- cell
- suspension
- cells
- separators
- stored
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title abstract 2
- 210000001744 T-lymphocyte Anatomy 0.000 abstract 6
- 210000003719 b-lymphocyte Anatomy 0.000 abstract 6
- 239000006285 cell suspension Substances 0.000 abstract 4
- 210000000265 leukocyte Anatomy 0.000 abstract 3
- 239000007788 liquid Substances 0.000 abstract 3
- 239000000725 suspension Substances 0.000 abstract 3
- 210000004698 lymphocyte Anatomy 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 1
- 238000005259 measurement Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Ecology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
PURPOSE:To enable a simple, rapid, and correct measurement of a proportion of T cell and B cell, by a method wherein T cell suspension and B cell suspension are obtained from a leukocyte suspension. CONSTITUTION:The leukocyte suspension, stored in a test liquid storage receptacle 5, is supplied in each equal amount into T cell separator 6 and B cell separator 7, and are caused to penetrate into a separating agent wherewith the separators are filled. Instantly, or after the suspension is kept in a stationary manner for a specific period of time, a cleansing liquid in a receptacle 4 is fed into the separators 6 and 7 where their respective separating agents are washed, and unabsorbed T cells and B cells are fed out of the separators 6 and 7 togetherwith the cleansing liquid to be stored in receptacles 8 and 9. The number of cells in the T cell suspension and B cell suspension, which are stored therein, are measured by means of a detector 2 and are inputted to an arithmetic unit 3 where the proportion of the T cell and B cell in the lymphocyte and leukocyte or in the lymphocyte are computed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5489880A JPS56151356A (en) | 1980-04-26 | 1980-04-26 | Method and device for measuring of t and b cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5489880A JPS56151356A (en) | 1980-04-26 | 1980-04-26 | Method and device for measuring of t and b cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS56151356A true JPS56151356A (en) | 1981-11-24 |
Family
ID=12983413
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5489880A Pending JPS56151356A (en) | 1980-04-26 | 1980-04-26 | Method and device for measuring of t and b cells |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS56151356A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4836928A (en) * | 1984-04-28 | 1989-06-06 | Terumo Kabushiki Kaisha | Separation method, separation device and separation apparatus for separating body fluid into respective components |
-
1980
- 1980-04-26 JP JP5489880A patent/JPS56151356A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4836928A (en) * | 1984-04-28 | 1989-06-06 | Terumo Kabushiki Kaisha | Separation method, separation device and separation apparatus for separating body fluid into respective components |
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