JPS5598349A - Determinating method of l-lysine - Google Patents
Determinating method of l-lysineInfo
- Publication number
- JPS5598349A JPS5598349A JP635879A JP635879A JPS5598349A JP S5598349 A JPS5598349 A JP S5598349A JP 635879 A JP635879 A JP 635879A JP 635879 A JP635879 A JP 635879A JP S5598349 A JPS5598349 A JP S5598349A
- Authority
- JP
- Japan
- Prior art keywords
- microorganism
- electrode
- film
- lysine
- carbon dioxide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 title abstract 9
- 244000005700 microbiome Species 0.000 abstract 12
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract 8
- 239000010408 film Substances 0.000 abstract 6
- 235000019766 L-Lysine Nutrition 0.000 abstract 4
- 239000004472 Lysine Substances 0.000 abstract 4
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract 4
- 239000001569 carbon dioxide Substances 0.000 abstract 4
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 238000005262 decarbonization Methods 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 2
- 229920000298 Cellophane Polymers 0.000 abstract 1
- 238000000502 dialysis Methods 0.000 abstract 1
- SDCJMBBHNJPYGW-UHFFFAOYSA-L disodium;hydrogen carbonate;chloride Chemical compound [Na+].[Na+].Cl.[O-]C([O-])=O SDCJMBBHNJPYGW-UHFFFAOYSA-L 0.000 abstract 1
- 230000002940 repellent Effects 0.000 abstract 1
- 239000005871 repellent Substances 0.000 abstract 1
- 238000007789 sealing Methods 0.000 abstract 1
- 239000010409 thin film Substances 0.000 abstract 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To make possible the determination of L-lysine simply and precisely by sealing a microorganism germ having L-lysine decarbonization enzyme activity and using a microorganism electrode having dissolved carbon dioxide gas film on which a microorganism film having a microorganism film fixed.
CONSTITUTION: A microorganism electrode 7 in which a microorganism germ 2 having a L-decarbonization enzyme activity is sealed between a diaphragm 1 of carbon dioxide gas electrode and a thin film 4 having a finely small opening covering thereon or a microorganism electrode 7 in which a microorganism germ fixing microorganism film is attached onto a diaphragm of dissolved carbon dioxide gas electrode is employed. This microorganism electrode 7 is immersed into L-lysine containing liquid to be tested or contacted therewith under an air repellent condition, and electromotive force is measured to determine L-lysine. For example, dissolved carbon dioxide gas electrode 1, a microorganism layer 2, a carrier 3 a dialysis film (cellophane film), pH electrode 6, an internal liquid 6 (NaHCO3-NaCl) constitute the microorganism electrode 7.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP54006358A JPS6042911B2 (en) | 1979-01-22 | 1979-01-22 | Assay method for L-lysine |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP54006358A JPS6042911B2 (en) | 1979-01-22 | 1979-01-22 | Assay method for L-lysine |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5598349A true JPS5598349A (en) | 1980-07-26 |
JPS6042911B2 JPS6042911B2 (en) | 1985-09-25 |
Family
ID=11636135
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP54006358A Expired JPS6042911B2 (en) | 1979-01-22 | 1979-01-22 | Assay method for L-lysine |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6042911B2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6976580B2 (en) | 2001-11-06 | 2005-12-20 | Mizuno Corporation | Golf bag with a stable base |
-
1979
- 1979-01-22 JP JP54006358A patent/JPS6042911B2/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6976580B2 (en) | 2001-11-06 | 2005-12-20 | Mizuno Corporation | Golf bag with a stable base |
Also Published As
Publication number | Publication date |
---|---|
JPS6042911B2 (en) | 1985-09-25 |
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