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JPS557006A - Purification of urokinase - Google Patents

Purification of urokinase

Info

Publication number
JPS557006A
JPS557006A JP7743978A JP7743978A JPS557006A JP S557006 A JPS557006 A JP S557006A JP 7743978 A JP7743978 A JP 7743978A JP 7743978 A JP7743978 A JP 7743978A JP S557006 A JPS557006 A JP S557006A
Authority
JP
Japan
Prior art keywords
column
urokinase
adsorbent
pyrogenous
substances
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP7743978A
Other languages
Japanese (ja)
Inventor
Hiroaki Nakamura
Izumi Kumita
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nippon Soda Co Ltd
Original Assignee
Nippon Soda Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Soda Co Ltd filed Critical Nippon Soda Co Ltd
Priority to JP7743978A priority Critical patent/JPS557006A/en
Publication of JPS557006A publication Critical patent/JPS557006A/en
Pending legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: To obtain high-purity urokinase free from pyrogenous substances, by a combination of gel filtration with a crosslinked dextran and affinity chromatography with urokinase adsorbent.
CONSTITUTION: Crude urokinase (U) obtained from human urines is dissolved in a phosphoric acid buffer and passed through a crosslinked dextran gel column. The effluent of pyrogenous substances is discharged from the column befor U. The column is then connected to a urokinase adsorbent column, wherein a group of the formula (R is amidino or guanidino group; n is an integer 5W12) is linked to a water-insoluble carrier. The pure U flows out of the column and is adsorbed to the adsorbent column.
COPYRIGHT: (C)1980,JPO&Japio
JP7743978A 1978-06-28 1978-06-28 Purification of urokinase Pending JPS557006A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP7743978A JPS557006A (en) 1978-06-28 1978-06-28 Purification of urokinase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP7743978A JPS557006A (en) 1978-06-28 1978-06-28 Purification of urokinase

Publications (1)

Publication Number Publication Date
JPS557006A true JPS557006A (en) 1980-01-18

Family

ID=13634045

Family Applications (1)

Application Number Title Priority Date Filing Date
JP7743978A Pending JPS557006A (en) 1978-06-28 1978-06-28 Purification of urokinase

Country Status (1)

Country Link
JP (1) JPS557006A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6127622A (en) * 1984-07-18 1986-02-07 Mitsubishi Metal Corp Formation of iii-v group compound semiconductor multilayer film
CN102167739A (en) * 2011-02-15 2011-08-31 南昌市浩然生物医药有限公司 Method for purifying human urinary trypsin inhibitor by utilizing salt resistant mixed mode adsorbent

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6127622A (en) * 1984-07-18 1986-02-07 Mitsubishi Metal Corp Formation of iii-v group compound semiconductor multilayer film
CN102167739A (en) * 2011-02-15 2011-08-31 南昌市浩然生物医药有限公司 Method for purifying human urinary trypsin inhibitor by utilizing salt resistant mixed mode adsorbent

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