JPS55162989A - Preparation of aldolase - Google Patents
Preparation of aldolaseInfo
- Publication number
- JPS55162989A JPS55162989A JP7121679A JP7121679A JPS55162989A JP S55162989 A JPS55162989 A JP S55162989A JP 7121679 A JP7121679 A JP 7121679A JP 7121679 A JP7121679 A JP 7121679A JP S55162989 A JPS55162989 A JP S55162989A
- Authority
- JP
- Japan
- Prior art keywords
- aldolase
- rice
- combined
- aqueous solvent
- 5mmol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: The embryo bud and glue powder layer (rice bran) of rice are extracted with an aqueous solvent to prepare aldolase inexpensively in a large amount.
CONSTITUTION: The embryo bud and glue powder layer (rice bran) of rice are combined with an aqueous solvent such as 50mmol tris(hydroxymethyl)aminomethane-hycrochloric acid buffer solution containing 5mmol of EDTA and 5mmol of β-mercaptoethanol to extract aldolase at 0°CWroom temperature. The aqueous extract is centrifuged to remove the upper layer of fat or oil and the precipitate and the middle layer of the extact is combined with salts such as sodium choride and magnesium sulfate to cause partition precipitation, thus giving colorless needles of aldolase with a molecular weight of about 156,500, an absorption maximum of ultraviolet spectrum at 278nm and an isoelectric point of 7.9pH.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7121679A JPS55162989A (en) | 1979-06-08 | 1979-06-08 | Preparation of aldolase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7121679A JPS55162989A (en) | 1979-06-08 | 1979-06-08 | Preparation of aldolase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS55162989A true JPS55162989A (en) | 1980-12-18 |
Family
ID=13454251
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7121679A Pending JPS55162989A (en) | 1979-06-08 | 1979-06-08 | Preparation of aldolase |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS55162989A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007103757A1 (en) * | 2006-03-03 | 2007-09-13 | Specialty Protein Producers, Inc. | Methods of separating fat from non-soy plant materials and compositions produced therefrom |
| US8163413B2 (en) | 2007-12-28 | 2012-04-24 | Fujitsu Limited | Electronic apparatus |
-
1979
- 1979-06-08 JP JP7121679A patent/JPS55162989A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007103757A1 (en) * | 2006-03-03 | 2007-09-13 | Specialty Protein Producers, Inc. | Methods of separating fat from non-soy plant materials and compositions produced therefrom |
| US10555542B2 (en) | 2006-03-03 | 2020-02-11 | Specialty Protein Producers, Inc. | Methods of separating fat from non-soy plant materials and compositions produced therefrom |
| US12193452B2 (en) | 2006-03-03 | 2025-01-14 | Specialty Protein Producers, Inc. | Methods of separating fat from non-soy plant materials and compositions produced therefrom |
| US8163413B2 (en) | 2007-12-28 | 2012-04-24 | Fujitsu Limited | Electronic apparatus |
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