JPS5515702A - New method of measuring activity of leucine aminopeptidase with new substrate - Google Patents
New method of measuring activity of leucine aminopeptidase with new substrateInfo
- Publication number
- JPS5515702A JPS5515702A JP8670578A JP8670578A JPS5515702A JP S5515702 A JPS5515702 A JP S5515702A JP 8670578 A JP8670578 A JP 8670578A JP 8670578 A JP8670578 A JP 8670578A JP S5515702 A JPS5515702 A JP S5515702A
- Authority
- JP
- Japan
- Prior art keywords
- substrate
- derivative
- new
- activity
- leucine aminopeptidase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: Using a new substrate of anilide derivative is measured the activity of leucine aminopeptidase in samples from living bodies, which is required for diangoses of acute hepatitis, hepatic cancer, metastatic hepatic cancer, hepato- cirrhosis, cholangia, etc.
CONSTITUTION: A derivative of L-leucyl-4-hydroxyanilide of the formula is used as the substrate and the 4-hydroxyaniline derivative formed by the action of LAP is condensed through oxidation to form a colored compound, which is colorimetrically determined, whereby the activity of LAP in samples from living bodies can be measured. The L-leucy 1-4-hydroxyanilide derivative, the substrate, is obtained by reacting usually activated L-leucine having protected amino group with p-hydroxyaniline derivative followed by removing the protecting group from the condensation product. As shown in the table, the substrate in the present invention is greatly excellent in substrate reactivity compared to conventional substrates.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8670578A JPS5515702A (en) | 1978-07-18 | 1978-07-18 | New method of measuring activity of leucine aminopeptidase with new substrate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8670578A JPS5515702A (en) | 1978-07-18 | 1978-07-18 | New method of measuring activity of leucine aminopeptidase with new substrate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5515702A true JPS5515702A (en) | 1980-02-04 |
Family
ID=13894336
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8670578A Pending JPS5515702A (en) | 1978-07-18 | 1978-07-18 | New method of measuring activity of leucine aminopeptidase with new substrate |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5515702A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4520100A (en) * | 1981-09-28 | 1985-05-28 | Nitto Boseki Co. Ltd. | Method for measuring thrombin |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54157691A (en) * | 1978-06-01 | 1979-12-12 | Nitto Boseki Co Ltd | Novel substrate for measuring enzyme activity |
-
1978
- 1978-07-18 JP JP8670578A patent/JPS5515702A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54157691A (en) * | 1978-06-01 | 1979-12-12 | Nitto Boseki Co Ltd | Novel substrate for measuring enzyme activity |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4520100A (en) * | 1981-09-28 | 1985-05-28 | Nitto Boseki Co. Ltd. | Method for measuring thrombin |
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