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JPS55131392A - Enzyme fixing membrane and its preparation - Google Patents

Enzyme fixing membrane and its preparation

Info

Publication number
JPS55131392A
JPS55131392A JP3744079A JP3744079A JPS55131392A JP S55131392 A JPS55131392 A JP S55131392A JP 3744079 A JP3744079 A JP 3744079A JP 3744079 A JP3744079 A JP 3744079A JP S55131392 A JPS55131392 A JP S55131392A
Authority
JP
Japan
Prior art keywords
membrane
enzyme
fixing
microholes
surface active
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP3744079A
Other languages
Japanese (ja)
Other versions
JPS5741238B2 (en
Inventor
Masakatsu Hado
Masao Suda
Akira Okata
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP3744079A priority Critical patent/JPS55131392A/en
Publication of JPS55131392A publication Critical patent/JPS55131392A/en
Publication of JPS5741238B2 publication Critical patent/JPS5741238B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

PURPOSE: An enzyme fixing membrane capable of being made into extremely then, having a sufficient mechanical strength, obtained by filling communicating microholes of porous Teflon membrane with an oxygen-containing gel substance.
CONSTITUTION: A porous Teflon-membrane is treated with an organic solvent having offinity (e.g., methanol) and processed with a surface active agent-containing aqueous solution. Excess surface active agent is removed if necessary, and the membrane is immersed in an aqueous solution having an enzyme (e.g., urease) and a fixing agent (e.g., a photosensitive resin) so that the communicating microholes are impregnated with the solution, which is submitted to gelation. The resulting enzyme fixing membrane is applied to the indicated electrode to construct an enzyme electrode, which has sufficient functions as a sensor with respect to response speed and long-term stability and exhibits stable output power in blood serum and whole blood while being hardly polluted.
COPYRIGHT: (C)1980,JPO&Japio
JP3744079A 1979-03-29 1979-03-29 Enzyme fixing membrane and its preparation Granted JPS55131392A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP3744079A JPS55131392A (en) 1979-03-29 1979-03-29 Enzyme fixing membrane and its preparation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP3744079A JPS55131392A (en) 1979-03-29 1979-03-29 Enzyme fixing membrane and its preparation

Publications (2)

Publication Number Publication Date
JPS55131392A true JPS55131392A (en) 1980-10-13
JPS5741238B2 JPS5741238B2 (en) 1982-09-02

Family

ID=12497562

Family Applications (1)

Application Number Title Priority Date Filing Date
JP3744079A Granted JPS55131392A (en) 1979-03-29 1979-03-29 Enzyme fixing membrane and its preparation

Country Status (1)

Country Link
JP (1) JPS55131392A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5896246A (en) * 1981-11-05 1983-06-08 マイルス・ラボラトリ−ズ・インコ−ポレ−テツド Method for producing enzyme membranes in which enzymes are protected and wrapped in capsules
US4539294A (en) * 1982-09-30 1985-09-03 Akzona Incorporated Immobilization of proteins on polymeric supports

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5896246A (en) * 1981-11-05 1983-06-08 マイルス・ラボラトリ−ズ・インコ−ポレ−テツド Method for producing enzyme membranes in which enzymes are protected and wrapped in capsules
US4539294A (en) * 1982-09-30 1985-09-03 Akzona Incorporated Immobilization of proteins on polymeric supports

Also Published As

Publication number Publication date
JPS5741238B2 (en) 1982-09-02

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