JPS55131389A - Purification of deoxyribonuclease i - Google Patents
Purification of deoxyribonuclease iInfo
- Publication number
- JPS55131389A JPS55131389A JP3843879A JP3843879A JPS55131389A JP S55131389 A JPS55131389 A JP S55131389A JP 3843879 A JP3843879 A JP 3843879A JP 3843879 A JP3843879 A JP 3843879A JP S55131389 A JPS55131389 A JP S55131389A
- Authority
- JP
- Japan
- Prior art keywords
- deoxyribonuclease
- deoxyribonucleic acid
- extracted
- column
- mammalian
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000007260 Deoxyribonuclease I Human genes 0.000 title abstract 4
- 108010008532 Deoxyribonuclease I Proteins 0.000 title abstract 4
- 238000000746 purification Methods 0.000 title 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract 3
- 102000053602 DNA Human genes 0.000 abstract 3
- 108020004414 DNA Proteins 0.000 abstract 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 abstract 3
- 239000007788 liquid Substances 0.000 abstract 3
- 229920000936 Agarose Polymers 0.000 abstract 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 abstract 2
- 210000000496 pancreas Anatomy 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108010067035 Pancrelipase Proteins 0.000 abstract 1
- 229920002684 Sepharose Polymers 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 abstract 1
- 238000004090 dissolution Methods 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 235000011164 potassium chloride Nutrition 0.000 abstract 1
- 239000001103 potassium chloride Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To purify the title enzyme in high purity with ease useful as reagents and drugs, by adsorbing deoxyribonuclease I in an extracted liquid of mammalian pancreas on a deoxyribonucleic acid fixed agarose, followed by its dissolution.
CONSTITUTION: Mammalian pancrease are minced, suspended in water, and extracted with an aqueous solution of acetic acid, etc. to give an extract-containing liquid. A deoxyribonucleic acid fixed agarose, obtained by fixing deoxyribonucleic acid on cyanogen bromide activated Sepharose, is filled in a column which is previously adjusted to a pH of about 4W5 with a buffer solution of citric acid. The pancreas extracted liquid is added to the column and deoxyribonuclease I is adsorbed selectively. The ionic strength is raised by the use of a potassium chloride solution to dissolve deoxyribonuclease I.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3843879A JPS55131389A (en) | 1979-04-02 | 1979-04-02 | Purification of deoxyribonuclease i |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3843879A JPS55131389A (en) | 1979-04-02 | 1979-04-02 | Purification of deoxyribonuclease i |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS55131389A true JPS55131389A (en) | 1980-10-13 |
| JPS6317436B2 JPS6317436B2 (en) | 1988-04-13 |
Family
ID=12525299
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3843879A Granted JPS55131389A (en) | 1979-04-02 | 1979-04-02 | Purification of deoxyribonuclease i |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS55131389A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0853121A3 (en) * | 1988-12-23 | 1998-08-05 | Genentech, Inc. | Human DNase |
-
1979
- 1979-04-02 JP JP3843879A patent/JPS55131389A/en active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0853121A3 (en) * | 1988-12-23 | 1998-08-05 | Genentech, Inc. | Human DNase |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6317436B2 (en) | 1988-04-13 |
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