JPS5484088A - Reaction catalyzed with immobilized enzyme - Google Patents
Reaction catalyzed with immobilized enzymeInfo
- Publication number
- JPS5484088A JPS5484088A JP15059277A JP15059277A JPS5484088A JP S5484088 A JPS5484088 A JP S5484088A JP 15059277 A JP15059277 A JP 15059277A JP 15059277 A JP15059277 A JP 15059277A JP S5484088 A JPS5484088 A JP S5484088A
- Authority
- JP
- Japan
- Prior art keywords
- polymer
- enzyme
- microbial cells
- immobilized enzyme
- polycationic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
PURPOSE: To prepare a repeatedly usable immobilized enzyme, easily, by adsorbing enzyme or microbial cells to a complex of a polycationic polymer and a poly anionic polymer.
CONSTITUTION: Enzyme or microbial cells having enzymatic activity is adsorbed to a water-insoluble polyion complex composed of a polycationic polymer such as poly (N-methyl-2-methyl-5-vinylpyridine)iodide (PMVP) and a polyanionic polymer such as polystyrene sulfonic acid potassium salt (PSSK), and the immobilized enzyme thus obtained is used for the enzymatic reaction. The adsorption is carried out by adding the polycationic polymer or the polyanionic polymer to an aqueous solution containing the enzyme or the microbial cells and an ionic polymer having opposite charge to the above polymer.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP15059277A JPS6017513B2 (en) | 1977-12-16 | 1977-12-16 | Immobilized enzyme reaction method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP15059277A JPS6017513B2 (en) | 1977-12-16 | 1977-12-16 | Immobilized enzyme reaction method |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5484088A true JPS5484088A (en) | 1979-07-04 |
JPS6017513B2 JPS6017513B2 (en) | 1985-05-02 |
Family
ID=15500242
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP15059277A Expired JPS6017513B2 (en) | 1977-12-16 | 1977-12-16 | Immobilized enzyme reaction method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6017513B2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS58187188A (en) * | 1982-04-27 | 1983-11-01 | Nippon Oil Co Ltd | Immobilization method of enzymic active substance |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6322590Y2 (en) * | 1985-02-07 | 1988-06-21 |
-
1977
- 1977-12-16 JP JP15059277A patent/JPS6017513B2/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS58187188A (en) * | 1982-04-27 | 1983-11-01 | Nippon Oil Co Ltd | Immobilization method of enzymic active substance |
Also Published As
Publication number | Publication date |
---|---|
JPS6017513B2 (en) | 1985-05-02 |
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