JPS54101485A - Preparation of novel nuclease - Google Patents
Preparation of novel nucleaseInfo
- Publication number
- JPS54101485A JPS54101485A JP12711878A JP12711878A JPS54101485A JP S54101485 A JPS54101485 A JP S54101485A JP 12711878 A JP12711878 A JP 12711878A JP 12711878 A JP12711878 A JP 12711878A JP S54101485 A JPS54101485 A JP S54101485A
- Authority
- JP
- Japan
- Prior art keywords
- dna
- extract
- bacillus sphaericus
- endonuclease
- bacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 101710163270 Nuclease Proteins 0.000 title abstract 2
- 108020004414 DNA Proteins 0.000 abstract 5
- 241000193386 Lysinibacillus sphaericus Species 0.000 abstract 3
- 241000894006 Bacteria Species 0.000 abstract 2
- 102000004533 Endonucleases Human genes 0.000 abstract 2
- 108010042407 Endonucleases Proteins 0.000 abstract 2
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 1
- 102000053602 DNA Human genes 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 241000701959 Escherichia virus Lambda Species 0.000 abstract 1
- 230000005526 G1 to G0 transition Effects 0.000 abstract 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 1
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 1
- 230000003698 anagen phase Effects 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 210000004748 cultured cell Anatomy 0.000 abstract 1
- 238000005194 fractionation Methods 0.000 abstract 1
- 238000002523 gelfiltration Methods 0.000 abstract 1
- 238000004255 ion exchange chromatography Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To prepare a nuclease having substrate specificity, which discriminates the DNA of specific species of organisms, and breaks the phosphodiester bond at a specific position of the DNA molecule, by separating and purifying an extract of bacterial cells obtained by the culture of bacteria belonging to Bacillus sphaericus.
CONSTITUTION: Endonuclease-producing bacteria belonging to bacillus sphaericus, e.g. Bacillus sphaericus IAM 1286 strain, are cultured in a nutrient medium at 30W37°C using an aerated and agitated fermentor. The cultured cells are collected between the logarithmic growth phase and the initial stage of the stationary phase, disintegrated and extracted to obtain a cell-free extract. The extract is purified by the combination of removal of nucleic acid, ammonium sulfate fractionation, ion exchange chromatography, gel filtration, etc. to obtain the objective endonuclease. The enzyme breaks the phosphodiester bond of Bacillus phage ϕ105C DNA and coil phage λ DNA to DNA fractions having specific lengths.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12711878A JPS54101485A (en) | 1978-10-16 | 1978-10-16 | Preparation of novel nuclease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12711878A JPS54101485A (en) | 1978-10-16 | 1978-10-16 | Preparation of novel nuclease |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8788376A Division JPS5315491A (en) | 1976-07-23 | 1976-07-23 | Preparation of novel nucleicacidase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS54101485A true JPS54101485A (en) | 1979-08-10 |
| JPS5625110B2 JPS5625110B2 (en) | 1981-06-10 |
Family
ID=14952042
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12711878A Granted JPS54101485A (en) | 1978-10-16 | 1978-10-16 | Preparation of novel nuclease |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS54101485A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS50121671A (en) * | 1974-03-07 | 1975-09-23 |
-
1978
- 1978-10-16 JP JP12711878A patent/JPS54101485A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS50121671A (en) * | 1974-03-07 | 1975-09-23 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5625110B2 (en) | 1981-06-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPS561890A (en) | Preparation of l-phenylalanine by fermentation | |
| JPS55162791A (en) | Antibiotic c-15003pnd and its preparation | |
| JPS54101484A (en) | Preparation of novel nuclease | |
| JPS54101485A (en) | Preparation of novel nuclease | |
| JPH01277495A (en) | Biological conversion of l-thirosine and l-phenylalanin to 2, 5-dihydroxyphenyl acetic acid | |
| JPS54101483A (en) | Preparation of novel nuclease | |
| JPS54101482A (en) | Preparation of novel nuclease | |
| JPS568687A (en) | Preparation of creatinase | |
| JPS5675097A (en) | Thermophilic aspartase and its preparation | |
| JPS5446887A (en) | Preparation of acrylic acid or methacrylic acid | |
| JPS5519058A (en) | Preparation of nuclease | |
| Kurane et al. | Removal of phthalate esters in soil column inoculated with microorganisms | |
| JPS5545322A (en) | Preparation of mitomycins by fermentation | |
| JPS55135588A (en) | Preparation of heat-resistant catalase | |
| JPS5658491A (en) | Immunoactivating substance ies-1638 having antitumor activity, and its preparation | |
| JPS56140888A (en) | Preparation of limiting enzyme | |
| JPS557015A (en) | Proline acylase, its preparation and preparation of l-proline | |
| JPS5675095A (en) | Preparation of heat-resistant acetic acid kinase | |
| SU1659480A1 (en) | Strain of bacterium klebsiella pneumoniae - a producer of restrictase kpn 378 1 | |
| JPS54126791A (en) | Preparation of pyruvate oxidase | |
| JPS54126789A (en) | Preparation of glutamic acid dehydrogenase | |
| JPS5571491A (en) | Preparation of proline acylase | |
| JPS5863387A (en) | Preparation of restriction enzyme | |
| JPS5519070A (en) | Preparation of benikoji pigment | |
| JPS6214788A (en) | Production of methacrylic acid |