JPH11501816A - 減少した吸着性と増加した加水分解性を有するサーミターゼ変種 - Google Patents

減少した吸着性と増加した加水分解性を有するサーミターゼ変種

Info

Publication number: JPH11501816A
Authority: JP; Japan
Prior art keywords: substituted amino; substitution occurs; glu; asp; amino acids
Prior art date: 1995-03-09
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

JP8527693A

Other languages

English (en)

Japanese (ja)

Inventor

フレデリックザ、サードブロウド，フィリップ

リーバーネット，ボビー

ネルトンラビング，ドン

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Procter and Gamble Co

Original Assignee

Procter and Gamble Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1995-03-09

Filing date

1996-03-06

Publication date

1999-02-16

1996-03-06 Application filed by Procter and Gamble Co filed Critical Procter and Gamble Co

1999-02-16 Publication of JPH11501816A publication Critical patent/JPH11501816A/ja

Status Pending legal-status Critical Current

Links

108010031354 thermitase Proteins 0.000 title claims abstract description 63
150000001413 amino acids Chemical class 0.000 claims abstract description 145
238000006467 substitution reaction Methods 0.000 claims abstract description 144
239000000203 mixture Substances 0.000 claims abstract description 139
238000004140 cleaning Methods 0.000 claims abstract description 103
239000000758 substrate Substances 0.000 claims abstract description 23
108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 7
125000003275 alpha amino acid group Chemical class 0.000 claims abstract 8
102000004190 Enzymes Human genes 0.000 claims description 84
108090000790 Enzymes Proteins 0.000 claims description 84
239000004744 fabric Substances 0.000 claims description 25
239000004094 surface-active agent Substances 0.000 claims description 14
-1 bleaches Substances 0.000 claims description 12
238000004851 dishwashing Methods 0.000 claims description 9
239000003795 chemical substances by application Substances 0.000 claims description 6
239000002131 composite material Substances 0.000 claims description 6
239000002689 soil Substances 0.000 claims description 5
239000003623 enhancer Substances 0.000 claims description 4
239000006260 foam Substances 0.000 claims description 4
239000003381 stabilizer Substances 0.000 claims description 3
239000002270 dispersing agent Substances 0.000 claims description 2
239000000049 pigment Substances 0.000 claims description 2
239000003205 fragrance Substances 0.000 claims 1
239000003112 inhibitor Substances 0.000 claims 1
230000002087 whitening effect Effects 0.000 claims 1
235000001014 amino acid Nutrition 0.000 description 85
229940088598 enzyme Drugs 0.000 description 82
108091005804 Peptidases Proteins 0.000 description 17
239000007788 liquid Substances 0.000 description 17
239000004365 Protease Substances 0.000 description 16
108090000623 proteins and genes Proteins 0.000 description 16
235000018102 proteins Nutrition 0.000 description 14
102000004169 proteins and genes Human genes 0.000 description 14
DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 13
239000003599 detergent Substances 0.000 description 12
238000006460 hydrolysis reaction Methods 0.000 description 10
238000001179 sorption measurement Methods 0.000 description 10
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 9
230000007062 hydrolysis Effects 0.000 description 9
239000007787 solid Substances 0.000 description 9
102000035195 Peptidases Human genes 0.000 description 8
108020004414 DNA Proteins 0.000 description 7
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
108090000787 Subtilisin Proteins 0.000 description 7
239000002904 solvent Substances 0.000 description 7
ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 6
230000000694 effects Effects 0.000 description 6
239000008187 granular material Substances 0.000 description 6
230000035772 mutation Effects 0.000 description 6
230000007935 neutral effect Effects 0.000 description 6
239000000243 solution Substances 0.000 description 6
239000002253 acid Substances 0.000 description 5
238000013459 approach Methods 0.000 description 5
238000000855 fermentation Methods 0.000 description 5
230000004151 fermentation Effects 0.000 description 5
238000005187 foaming Methods 0.000 description 5
238000000034 method Methods 0.000 description 5
238000012986 modification Methods 0.000 description 5
230000004048 modification Effects 0.000 description 5
235000013772 propylene glycol Nutrition 0.000 description 5
244000063299 Bacillus subtilis Species 0.000 description 4
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
239000007983 Tris buffer Substances 0.000 description 4
239000007844 bleaching agent Substances 0.000 description 4
238000006243 chemical reaction Methods 0.000 description 4
MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical group OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 4
238000002474 experimental method Methods 0.000 description 4
238000004519 manufacturing process Methods 0.000 description 4
239000013612 plasmid Substances 0.000 description 4
239000011550 stock solution Substances 0.000 description 4
239000000126 substance Substances 0.000 description 4
239000003826 tablet Substances 0.000 description 4
239000000606 toothpaste Substances 0.000 description 4
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
208000035404 Autolysis Diseases 0.000 description 3
235000014469 Bacillus subtilis Nutrition 0.000 description 3
206010057248 Cell death Diseases 0.000 description 3
241000588724 Escherichia coli Species 0.000 description 3
LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
102220640248 Mitochondrial 2-oxoglutarate/malate carrier protein_S220E_mutation Human genes 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
238000002835 absorbance Methods 0.000 description 3
125000000129 anionic group Chemical group 0.000 description 3
239000000872 buffer Substances 0.000 description 3
239000000969 carrier Substances 0.000 description 3
150000001875 compounds Chemical class 0.000 description 3
230000007423 decrease Effects 0.000 description 3
229940079593 drug Drugs 0.000 description 3
239000003814 drug Substances 0.000 description 3
238000009472 formulation Methods 0.000 description 3
KFZMGEQAYNKOFK-UHFFFAOYSA-N isopropyl alcohol Natural products CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
108090000765 processed proteins & peptides Proteins 0.000 description 3
239000000047 product Substances 0.000 description 3
230000028043 self proteolysis Effects 0.000 description 3
238000002741 site-directed mutagenesis Methods 0.000 description 3
239000007921 spray Substances 0.000 description 3
229940034610 toothpaste Drugs 0.000 description 3
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
229940035893 uracil Drugs 0.000 description 3
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 2
108020004705 Codon Proteins 0.000 description 2
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
239000007987 MES buffer Substances 0.000 description 2
102220477261 Meiosis 1 arrest protein_T195P_mutation Human genes 0.000 description 2
KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 2
102220542207 Probable polypeptide N-acetylgalactosaminyltransferase 8_S192D_mutation Human genes 0.000 description 2
102220558516 Proteinase-activated receptor 2_Y210L_mutation Human genes 0.000 description 2
108010022999 Serine Proteases Proteins 0.000 description 2
102000012479 Serine Proteases Human genes 0.000 description 2
102220515246 Serum response factor-binding protein 1_T195N_mutation Human genes 0.000 description 2
CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
102220555361 X-box-binding protein 1_T212N_mutation Human genes 0.000 description 2
239000012190 activator Substances 0.000 description 2
150000008051 alkyl sulfates Chemical class 0.000 description 2
150000001412 amines Chemical class 0.000 description 2
238000004458 analytical method Methods 0.000 description 2
238000003556 assay Methods 0.000 description 2
230000008901 benefit Effects 0.000 description 2
229960003237 betaine Drugs 0.000 description 2
238000004587 chromatography analysis Methods 0.000 description 2
238000010367 cloning Methods 0.000 description 2
239000000551 dentifrice Substances 0.000 description 2
238000011161 development Methods 0.000 description 2
239000012634 fragment Substances 0.000 description 2
239000011521 glass Substances 0.000 description 2
DCPMPXBYPZGNDC-UHFFFAOYSA-N hydron;methanediimine;chloride Chemical compound Cl.N=C=N DCPMPXBYPZGNDC-UHFFFAOYSA-N 0.000 description 2
238000000338 in vitro Methods 0.000 description 2
238000010348 incorporation Methods 0.000 description 2
239000012528 membrane Substances 0.000 description 2
239000002324 mouth wash Substances 0.000 description 2
229940051866 mouthwash Drugs 0.000 description 2
238000002703 mutagenesis Methods 0.000 description 2
231100000350 mutagenesis Toxicity 0.000 description 2
239000002773 nucleotide Substances 0.000 description 2
125000003729 nucleotide group Chemical group 0.000 description 2
230000001590 oxidative effect Effects 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
102000004196 processed proteins & peptides Human genes 0.000 description 2
102220076508 rs150907052 Human genes 0.000 description 2
102220020329 rs397508119 Human genes 0.000 description 2
102200062430 rs397514559 Human genes 0.000 description 2
102200080721 rs398122362 Human genes 0.000 description 2
102200061605 rs63750569 Human genes 0.000 description 2
150000003333 secondary alcohols Chemical class 0.000 description 2
239000013598 vector Substances 0.000 description 2
238000005406 washing Methods 0.000 description 2
DNIAPMSPPWPWGF-VKHMYHEASA-N (+)-propylene glycol Chemical compound C[C@H](O)CO DNIAPMSPPWPWGF-VKHMYHEASA-N 0.000 description 1
DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 1
PSBDWGZCVUAZQS-UHFFFAOYSA-N (dimethylsulfonio)acetate Chemical compound C[S+](C)CC([O-])=O PSBDWGZCVUAZQS-UHFFFAOYSA-N 0.000 description 1
YPFDHNVEDLHUCE-UHFFFAOYSA-N 1,3-propanediol Substances OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 description 1
CUVLMZNMSPJDON-UHFFFAOYSA-N 1-(1-butoxypropan-2-yloxy)propan-2-ol Chemical compound CCCCOCC(C)OCC(C)O CUVLMZNMSPJDON-UHFFFAOYSA-N 0.000 description 1
GHPCICSQWQDZLM-UHFFFAOYSA-N 1-(4-chlorophenyl)sulfonyl-1-methyl-3-propylurea Chemical group CCCNC(=O)N(C)S(=O)(=O)C1=CC=C(Cl)C=C1 GHPCICSQWQDZLM-UHFFFAOYSA-N 0.000 description 1
RWNUSVWFHDHRCJ-UHFFFAOYSA-N 1-butoxypropan-2-ol Chemical compound CCCCOCC(C)O RWNUSVWFHDHRCJ-UHFFFAOYSA-N 0.000 description 1
MPJQXAIKMSKXBI-UHFFFAOYSA-N 2,7,9,14-tetraoxa-1,8-diazabicyclo[6.6.2]hexadecane-3,6,10,13-tetrone Chemical compound C1CN2OC(=O)CCC(=O)ON1OC(=O)CCC(=O)O2 MPJQXAIKMSKXBI-UHFFFAOYSA-N 0.000 description 1
CFPOJWPDQWJEMO-UHFFFAOYSA-N 2-(1,2-dicarboxyethoxy)butanedioic acid Chemical class OC(=O)CC(C(O)=O)OC(C(O)=O)CC(O)=O CFPOJWPDQWJEMO-UHFFFAOYSA-N 0.000 description 1
RWLALWYNXFYRGW-UHFFFAOYSA-N 2-Ethyl-1,3-hexanediol Chemical compound CCCC(O)C(CC)CO RWLALWYNXFYRGW-UHFFFAOYSA-N 0.000 description 1
XJFPXLWGZWAWRQ-UHFFFAOYSA-N 2-[[2-[[2-[[2-[[2-[(2-azaniumylacetyl)amino]acetyl]amino]acetyl]amino]acetyl]amino]acetyl]amino]acetate Chemical compound NCC(=O)NCC(=O)NCC(=O)NCC(=O)NCC(=O)NCC(O)=O XJFPXLWGZWAWRQ-UHFFFAOYSA-N 0.000 description 1
POAOYUHQDCAZBD-UHFFFAOYSA-N 2-butoxyethanol Chemical compound CCCCOCCO POAOYUHQDCAZBD-UHFFFAOYSA-N 0.000 description 1
UPGSWASWQBLSKZ-UHFFFAOYSA-N 2-hexoxyethanol Chemical compound CCCCCCOCCO UPGSWASWQBLSKZ-UHFFFAOYSA-N 0.000 description 1
GJHIOWXZFDVUKQ-AWEZNQCLSA-N 2360-97-6 Chemical compound C([C@H](N)C(=O)NC=1C=CC(=CC=1)[N+]([O-])=O)C1=CC=CC=C1 GJHIOWXZFDVUKQ-AWEZNQCLSA-N 0.000 description 1
TYMLOMAKGOJONV-UHFFFAOYSA-N 4-nitroaniline Chemical compound NC1=CC=C([N+]([O-])=O)C=C1 TYMLOMAKGOJONV-UHFFFAOYSA-N 0.000 description 1
QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
102220478239 Alpha-endosulfine_S109E_mutation Human genes 0.000 description 1
239000004382 Amylase Substances 0.000 description 1
102000013142 Amylases Human genes 0.000 description 1
108010065511 Amylases Proteins 0.000 description 1
108090000145 Bacillolysin Proteins 0.000 description 1
241000193830 Bacillus <bacterium> Species 0.000 description 1
101100228206 Caenorhabditis elegans gly-6 gene Proteins 0.000 description 1
101100505161 Caenorhabditis elegans mel-32 gene Proteins 0.000 description 1
CBOCVOKPQGJKKJ-UHFFFAOYSA-L Calcium formate Chemical compound [Ca+2].[O-]C=O.[O-]C=O CBOCVOKPQGJKKJ-UHFFFAOYSA-L 0.000 description 1
235000014036 Castanea Nutrition 0.000 description 1
241001070941 Castanea Species 0.000 description 1
108010059892 Cellulase Proteins 0.000 description 1
238000001712 DNA sequencing Methods 0.000 description 1
102220568475 Dual specificity mitogen-activated protein kinase kinase 1_S222D_mutation Human genes 0.000 description 1
102220471019 Dual specificity mitogen-activated protein kinase kinase 6_S207E_mutation Human genes 0.000 description 1
102220612105 E3 ubiquitin-protein ligase Topors_T224P_mutation Human genes 0.000 description 1
102000002322 Egg Proteins Human genes 0.000 description 1
108010000912 Egg Proteins Proteins 0.000 description 1
102220603318 Homeobox protein SIX3_V138D_mutation Human genes 0.000 description 1
206010020751 Hypersensitivity Diseases 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
102220510033 Importin-7_T111N_mutation Human genes 0.000 description 1
239000004367 Lipase Substances 0.000 description 1
102000004882 Lipase Human genes 0.000 description 1
108090001060 Lipase Proteins 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
240000002853 Nelumbo nucifera Species 0.000 description 1
235000006508 Nelumbo nucifera Nutrition 0.000 description 1
235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
102220481793 Neutral amino acid transporter A_A164G_mutation Human genes 0.000 description 1
102000035092 Neutral proteases Human genes 0.000 description 1
108091005507 Neutral proteases Proteins 0.000 description 1
239000006057 Non-nutritive feed additive Substances 0.000 description 1
102220547866 Nucleobindin-1_N140Q_mutation Human genes 0.000 description 1
108091034117 Oligonucleotide Proteins 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
101150004094 PRO2 gene Proteins 0.000 description 1
SHAQGFGGJSLLHE-BQBZGAKWSA-N Pro-Gln Chemical compound NC(=O)CC[C@@H](C([O-])=O)NC(=O)[C@@H]1CCC[NH2+]1 SHAQGFGGJSLLHE-BQBZGAKWSA-N 0.000 description 1
241000208474 Protea Species 0.000 description 1
239000004280 Sodium formate Substances 0.000 description 1
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
229920002472 Starch Polymers 0.000 description 1
108010056079 Subtilisins Proteins 0.000 description 1
102000005158 Subtilisins Human genes 0.000 description 1
101710162629 Trypsin inhibitor Proteins 0.000 description 1
229940122618 Trypsin inhibitor Drugs 0.000 description 1
229910021536 Zeolite Inorganic materials 0.000 description 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
238000011481 absorbance measurement Methods 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
239000004480 active ingredient Substances 0.000 description 1
239000000654 additive Substances 0.000 description 1
230000000996 additive effect Effects 0.000 description 1
238000005377 adsorption chromatography Methods 0.000 description 1
230000000274 adsorptive effect Effects 0.000 description 1
150000001408 amides Chemical class 0.000 description 1
235000019418 amylase Nutrition 0.000 description 1
239000003945 anionic surfactant Substances 0.000 description 1
239000002518 antifoaming agent Substances 0.000 description 1
230000000890 antigenic effect Effects 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
230000003115 biocidal effect Effects 0.000 description 1
238000004061 bleaching Methods 0.000 description 1
229940044172 calcium formate Drugs 0.000 description 1
235000019255 calcium formate Nutrition 0.000 description 1
239000004281 calcium formate Substances 0.000 description 1
238000004364 calculation method Methods 0.000 description 1
229940041514 candida albicans extract Drugs 0.000 description 1
125000004432 carbon atom Chemical group C* 0.000 description 1
239000005018 casein Substances 0.000 description 1
BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
235000021240 caseins Nutrition 0.000 description 1
239000003054 catalyst Substances 0.000 description 1
230000003197 catalytic effect Effects 0.000 description 1
238000006555 catalytic reaction Methods 0.000 description 1
150000001768 cations Chemical class 0.000 description 1
229940106157 cellulase Drugs 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
230000008859 change Effects 0.000 description 1
238000012512 characterization method Methods 0.000 description 1
239000002738 chelating agent Substances 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
229940112822 chewing gum Drugs 0.000 description 1
235000015218 chewing gum Nutrition 0.000 description 1
210000000991 chicken egg Anatomy 0.000 description 1
150000001860 citric acid derivatives Chemical class 0.000 description 1
239000004927 clay Substances 0.000 description 1
238000003776 cleavage reaction Methods 0.000 description 1
239000007822 coupling agent Substances 0.000 description 1
238000012217 deletion Methods 0.000 description 1
230000037430 deletion Effects 0.000 description 1
238000000502 dialysis Methods 0.000 description 1
235000014113 dietary fatty acids Nutrition 0.000 description 1
GSPKZYJPUDYKPI-UHFFFAOYSA-N diethoxy sulfate Chemical compound CCOOS(=O)(=O)OOCC GSPKZYJPUDYKPI-UHFFFAOYSA-N 0.000 description 1
229940028356 diethylene glycol monobutyl ether Drugs 0.000 description 1
125000002147 dimethylamino group Chemical group [H]C([H])([H])N(*)C([H])([H])[H] 0.000 description 1
150000002009 diols Chemical class 0.000 description 1
HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
239000012154 double-distilled water Substances 0.000 description 1
239000003937 drug carrier Substances 0.000 description 1
239000000975 dye Substances 0.000 description 1
235000013399 edible fruits Nutrition 0.000 description 1
239000007938 effervescent tablet Substances 0.000 description 1
235000014103 egg white Nutrition 0.000 description 1
238000010828 elution Methods 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
230000007071 enzymatic hydrolysis Effects 0.000 description 1
238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
230000009144 enzymatic modification Effects 0.000 description 1
150000002148 esters Chemical class 0.000 description 1
CCIVGXIOQKPBKL-UHFFFAOYSA-M ethanesulfonate Chemical compound CCS([O-])(=O)=O CCIVGXIOQKPBKL-UHFFFAOYSA-M 0.000 description 1
RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 1
125000001301 ethoxy group Chemical group [H]C([H])([H])C([H])([H])O* 0.000 description 1
229940071087 ethylenediamine disuccinate Drugs 0.000 description 1
238000001704 evaporation Methods 0.000 description 1
230000008020 evaporation Effects 0.000 description 1
230000007717 exclusion Effects 0.000 description 1
239000002979 fabric softener Substances 0.000 description 1
239000000194 fatty acid Substances 0.000 description 1
229930195729 fatty acid Natural products 0.000 description 1
150000004665 fatty acids Chemical class 0.000 description 1
239000012467 final product Substances 0.000 description 1
239000010419 fine particle Substances 0.000 description 1
230000037433 frameshift Effects 0.000 description 1
239000000499 gel Substances 0.000 description 1
230000002068 genetic effect Effects 0.000 description 1
239000008103 glucose Substances 0.000 description 1
235000011187 glycerol Nutrition 0.000 description 1
238000003306 harvesting Methods 0.000 description 1
230000003301 hydrolyzing effect Effects 0.000 description 1
239000003752 hydrotrope Substances 0.000 description 1
125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
229940060367 inert ingredients Drugs 0.000 description 1
239000004615 ingredient Substances 0.000 description 1
230000007794 irritation Effects 0.000 description 1
238000012933 kinetic analysis Methods 0.000 description 1
238000012417 linear regression Methods 0.000 description 1
235000019421 lipase Nutrition 0.000 description 1
239000007937 lozenge Substances 0.000 description 1
159000000003 magnesium salts Chemical class 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
239000000463 material Substances 0.000 description 1
238000005259 measurement Methods 0.000 description 1
IZXGZAJMDLJLMF-UHFFFAOYSA-N methylaminomethanol Chemical compound CNCO IZXGZAJMDLJLMF-UHFFFAOYSA-N 0.000 description 1
238000002156 mixing Methods 0.000 description 1
CQDGTJPVBWZJAZ-UHFFFAOYSA-N monoethyl carbonate Chemical compound CCOC(O)=O CQDGTJPVBWZJAZ-UHFFFAOYSA-N 0.000 description 1
231100000219 mutagenic Toxicity 0.000 description 1
230000003505 mutagenic effect Effects 0.000 description 1
229910052757 nitrogen Inorganic materials 0.000 description 1
238000003199 nucleic acid amplification method Methods 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
150000007523 nucleic acids Chemical class 0.000 description 1
229920002601 oligoester Polymers 0.000 description 1
229940100690 oral cream Drugs 0.000 description 1
JCGNDDUYTRNOFT-UHFFFAOYSA-N oxolane-2,4-dione Chemical compound O=C1COC(=O)C1 JCGNDDUYTRNOFT-UHFFFAOYSA-N 0.000 description 1
239000003002 pH adjusting agent Substances 0.000 description 1
239000006072 paste Substances 0.000 description 1
235000021317 phosphate Nutrition 0.000 description 1
PTMHPRAIXMAOOB-UHFFFAOYSA-N phosphoramidic acid Chemical class NP(O)(O)=O PTMHPRAIXMAOOB-UHFFFAOYSA-N 0.000 description 1
150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
229920005862 polyol Polymers 0.000 description 1
150000003077 polyols Chemical class 0.000 description 1
229920001296 polysiloxane Polymers 0.000 description 1
229920000166 polytrimethylene carbonate Polymers 0.000 description 1
230000003449 preventive effect Effects 0.000 description 1
BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
125000002572 propoxy group Chemical group [*]OC([H])([H])C(C([H])([H])[H])([H])[H] 0.000 description 1
238000001742 protein purification Methods 0.000 description 1
239000012460 protein solution Substances 0.000 description 1
230000002797 proteolythic effect Effects 0.000 description 1
238000010926 purge Methods 0.000 description 1
238000000746 purification Methods 0.000 description 1
238000011403 purification operation Methods 0.000 description 1
238000011160 research Methods 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
102220196096 rs1057518583 Human genes 0.000 description 1
102220201851 rs143406017 Human genes 0.000 description 1
102220317546 rs146895834 Human genes 0.000 description 1
102220068474 rs149348130 Human genes 0.000 description 1
102200142166 rs35258119 Human genes 0.000 description 1
102200047013 rs61752144 Human genes 0.000 description 1
102220092655 rs876657790 Human genes 0.000 description 1
102220097941 rs876659089 Human genes 0.000 description 1
102220293166 rs918796736 Human genes 0.000 description 1
230000007017 scission Effects 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
235000004400 serine Nutrition 0.000 description 1
150000003355 serines Chemical class 0.000 description 1
150000004760 silicates Chemical class 0.000 description 1
229910021647 smectite Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
229910000029 sodium carbonate Inorganic materials 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
HLBBKKJFGFRGMU-UHFFFAOYSA-M sodium formate Chemical compound [Na+].[O-]C=O HLBBKKJFGFRGMU-UHFFFAOYSA-M 0.000 description 1
235000019254 sodium formate Nutrition 0.000 description 1
239000008107 starch Substances 0.000 description 1
235000019698 starch Nutrition 0.000 description 1
238000003860 storage Methods 0.000 description 1
229940117986 sulfobetaine Drugs 0.000 description 1
150000003462 sulfoxides Chemical class 0.000 description 1
FRPJTGXMTIIFIT-UHFFFAOYSA-N tetraacetylethylenediamine Chemical compound CC(=O)C(N)(C(C)=O)C(N)(C(C)=O)C(C)=O FRPJTGXMTIIFIT-UHFFFAOYSA-N 0.000 description 1
FAGUFWYHJQFNRV-UHFFFAOYSA-N tetraethylenepentamine Chemical class NCCNCCNCCNCCN FAGUFWYHJQFNRV-UHFFFAOYSA-N 0.000 description 1
239000010409 thin film Substances 0.000 description 1
238000004448 titration Methods 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
230000002588 toxic effect Effects 0.000 description 1
239000011573 trace mineral Substances 0.000 description 1
235000013619 trace mineral Nutrition 0.000 description 1
230000009466 transformation Effects 0.000 description 1
239000002753 trypsin inhibitor Substances 0.000 description 1
210000000707 wrist Anatomy 0.000 description 1
239000012138 yeast extract Substances 0.000 description 1
239000010457 zeolite Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/0005—Other compounding ingredients characterised by their effect
- C11D3/0078—Compositions for cleaning contact lenses, spectacles or lenses
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Organic Chemistry (AREA)
General Health & Medical Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Zoology (AREA)
Genetics & Genomics (AREA)
Animal Behavior & Ethology (AREA)
Oil, Petroleum & Natural Gas (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Bioinformatics & Cheminformatics (AREA)
Oral & Maxillofacial Surgery (AREA)
Molecular Biology (AREA)
Medicinal Chemistry (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Epidemiology (AREA)
Microbiology (AREA)
Birds (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Detergent Compositions (AREA)

JP8527693A 1995-03-09 1996-03-06 減少した吸着性と増加した加水分解性を有するサーミターゼ変種 Pending JPH11501816A (ja)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US40157395A	1995-03-09	1995-03-09
US08/401,573		1995-03-09
PCT/US1996/003009 WO1996028558A1 (en)	1995-03-09	1996-03-06	Thermitase variants having decreased adsorption and increased hydrolysis

Publications (1)

Publication Number	Publication Date
JPH11501816A true JPH11501816A (ja)	1999-02-16

Family

ID=23588283

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP8527693A Pending JPH11501816A (ja)	1995-03-09	1996-03-06	減少した吸着性と増加した加水分解性を有するサーミターゼ変種

Country Status (14)

Country	Link
EP (1)	EP0815244A1 (zh)
JP (1)	JPH11501816A (zh)
CN (1)	CN1183118A (zh)
AU (1)	AU5182996A (zh)
BR (1)	BR9607756A (zh)
CA (1)	CA2214578A1 (zh)
CZ (1)	CZ280097A3 (zh)
HU (1)	HUP9802069A3 (zh)
IL (1)	IL117352A0 (zh)
IN (1)	IN187236B (zh)
MA (1)	MA23819A1 (zh)
MX (1)	MX9706846A (zh)
WO (1)	WO1996028558A1 (zh)
ZA (1)	ZA961822B (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2008013611A (ja) *	2006-07-03	2008-01-24	Kobayashi Pharmaceut Co Ltd	漂白洗浄剤組成物

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1997023605A1 (en) *	1995-12-22	1997-07-03	Helix Biotechnology Ltd.	Thermostable proteolytic enzyme from thermoactinomyces thalpophilus thm1
JP2001503269A (ja) *	1996-11-04	2001-03-13	ノボノルディスクアクティーゼルスカブ	ズブチラーゼ変異体及び、組成物
JPH10204500A (ja) *	1997-01-20	1998-08-04	Soft Kyukyu Corp:Kk	発泡錠剤洗浄剤
AR015977A1 (es) *	1997-10-23	2001-05-30	Genencor Int	Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes
ES2367505T3 (es)	1997-10-23	2011-11-04	Danisco Us Inc.	Variantes de proteasa con sustituciones múltiples con carga neta alterada para usar en detergentes.
BRPI9916351B1 (pt) *	1998-12-18	2016-04-12	Novozymes As	enzima de subtilase isolada, composição, e, uso de uma enzima subtilase isolada ou de uma composição de enzima
WO2000037627A1 (en) *	1998-12-18	2000-06-29	Novozymes A/S	Subtilase enzymes of the i-s1 and i-s2 sub-groups having an additional amino acid residue in an active site loop region
US7319112B2 (en)	2000-07-14	2008-01-15	The Procter & Gamble Co.	Non-halogenated antibacterial agents and processes for making same
DE10153792A1 (de)	2001-10-31	2003-05-22	Henkel Kgaa	Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DE10162728A1 (de)	2001-12-20	2003-07-10	Henkel Kgaa	Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162727A1 (de)	2001-12-20	2003-07-10	Henkel Kgaa	Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5116741A (en) *	1988-04-12	1992-05-26	Genex Corporation	Biosynthetic uses of thermostable proteases
DK316989D0 (da) *	1989-06-26	1989-06-26	Novo Nordisk As	Enzymer
ES2227508T3 (es) *	1989-06-26	2005-04-01	Unilever N.V.	Composiciones detergentes enzimaticas.
BR9006832A (pt) *	1989-06-26	1991-08-06	Unilever Nv	Composicao detergente enzimatica
ES2275265T3 (es) *	1993-09-15	2007-06-01	THE PROCTER & GAMBLE COMPANY	Variantes de subtilisina bpn con adsorcion reducida e hidrolisis mejorada.

1996
- 1996-03-04 IL IL11735296A patent/IL117352A0/xx unknown
- 1996-03-06 JP JP8527693A patent/JPH11501816A/ja active Pending
- 1996-03-06 CA CA002214578A patent/CA2214578A1/en not_active Abandoned
- 1996-03-06 IN IN457DE1996 patent/IN187236B/en unknown
- 1996-03-06 CZ CZ972800A patent/CZ280097A3/cs unknown
- 1996-03-06 WO PCT/US1996/003009 patent/WO1996028558A1/en not_active Application Discontinuation
- 1996-03-06 ZA ZA961822A patent/ZA961822B/xx unknown
- 1996-03-06 HU HU9802069A patent/HUP9802069A3/hu unknown
- 1996-03-06 EP EP96908663A patent/EP0815244A1/en not_active Withdrawn
- 1996-03-06 CN CN96193608A patent/CN1183118A/zh active Pending
- 1996-03-06 MX MX9706846A patent/MX9706846A/es unknown
- 1996-03-06 AU AU51829/96A patent/AU5182996A/en not_active Abandoned
- 1996-03-06 BR BR9607756A patent/BR9607756A/pt not_active Application Discontinuation
- 1996-03-07 MA MA24176A patent/MA23819A1/fr unknown

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2008013611A (ja) *	2006-07-03	2008-01-24	Kobayashi Pharmaceut Co Ltd	漂白洗浄剤組成物

Also Published As

Publication number	Publication date
MX9706846A (es)	1997-11-29
BR9607756A (pt)	1999-03-30
EP0815244A1 (en)	1998-01-07
MA23819A1 (fr)	1996-10-01
HUP9802069A3 (en)	2000-11-28
IN187236B (zh)	2002-03-09
WO1996028558A1 (en)	1996-09-19
CZ280097A3 (cs)	1998-01-14
IL117352A0 (en)	1996-07-23
HUP9802069A2 (hu)	1998-12-28
CA2214578A1 (en)	1996-09-19
CN1183118A (zh)	1998-05-27
AU5182996A (en)	1996-10-02
ZA961822B (en)	1996-09-11

Publication	Publication Date	Title
JPH11501812A (ja)	1999-02-16	減少した吸着性と増加した加水分解性を有するズブチリシンＣａｒｌｓｂｅｒｇ変種
JPH11501814A (ja)	1999-02-16	減少した吸着性と増加した加水分解性を有するプロテイナーゼｋ変種
JPH09511652A (ja)	1997-11-25	減少した吸着性と増加した加水分解性を有するズブチリシンｂｐｎ′変異体
JPH09512434A (ja)	1997-12-16	減少した吸着性と増加した加水分解性を有するズブチリシン３０９変異体
JPH09502610A (ja)	1997-03-18	吸着性が減少して加水分解性が増加したズブチリシンｂｐｎ′変異体
JPH09512433A (ja)	1997-12-16	ズブチリシンｂｐｎ′変異体を含む布帛クリーニング組成物
JPH11501815A (ja)	1999-02-16	減少した吸着性と増加した加水分解性を有するズブチリシンｄｙ変種
JP2002507426A (ja)	2002-03-12	アミノ酸置換を有するセリンプロテアーゼ変異体
JPH11501816A (ja)	1999-02-16	減少した吸着性と増加した加水分解性を有するサーミターゼ変種
WO1996028558A9 (en)	1997-01-16	Thermitase variants having decreased adsorption and increased hydrolysis
MXPA02000842A (es)	2002-07-30	Conjugados de proteasa que tienen sitios de corte protegidos estericamente.
US6440717B1 (en)	2002-08-27	BPN′ variants having decreased adsorption and increased hydrolysis
EP1196546A2 (en)	2002-04-17	Intramolecularly cross-linked subtilisin proteases having reduced immunogenicity

JPH11501816A - 減少した吸着性と増加した加水分解性を有するサーミターゼ変種 - Google Patents

Info

Links

Classifications

Landscapes

Applications Claiming Priority (3)

Publications (1)

Family

ID=23588283

Family Applications (1)

Country Status (14)

Cited By (1)

Families Citing this family (11)

Family Cites Families (5)

Cited By (1)

Also Published As

Similar Documents