JPH1149690A - Composition for stimulating lipolysis - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a lipolysis-stimulating action composition capable of being utilized as a new type dietary health food high in safety by compounding the pericarp extract of a specific fruit as a main ingredient. SOLUTION: This composition for stimulating lipolysis contains a water extract or a low boiling point organic solvent extract of the pericarp of a citrus fruit as a main ingredient. The composition is obtained e.g. by adding hot water to the dry pericarps of the citrous fruit (especially preferably Citrus Unshu Peel) and extracting under heating, or adding a low boiling point organic solvent and extracting under stirring, if necessary, squeezing with a hydraulic pressure machine, etc., removing insolubles by a method such as a centrifugal precipitation method or a filtration method, concentrating the filtrate, and subsequently pulverizing the filtrate by a lyophilization method, etc. The composition has an obesity-improving effect or a weight increase-suppressing effect.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for promoting lipolysis, which comprises an extract of citrus rind such as rind as a main component and has an effect of improving obesity or suppressing weight gain by its lipolysis promoting action. Thus, it can be used as a new type of diet health food that is different from the way the conventional diet health food works and that is highly safe.

[0002]

2. Description of the Related Art Citrus peels such as rind have been blended and used as crude drugs in Chinese medicine for the purpose of digesting stomach and removing antitussive. However, it is rarely used for other purposes.

On the other hand, recently, many people want to lose weight for health and beauty, and there are many diets for this purpose, that is, various kinds of health foods (diet health foods) for the purpose of weight loss. It is commercially available. These diet health foods are diverse, but the main ones are, for example, those mainly composed of low-calorie foods, those mainly composed of dietary fiber, and those mainly having a sweating action And those intended to reduce the amount of meal by giving a feeling of fullness by expanding in the stomach after eating.

[0004] However, these conventional diet health foods are intended to reduce the amount of calories ingested by consuming a large amount of them and reducing the amount of normal diet, thereby reducing weight. It does not act directly on fat accumulated in the body. Therefore, if you stop taking these conventional diet health foods, you will eat more than ever before, in order to obtain the same feeling of fullness as you continue to take, and as a result, you will eat even more than before you started taking diet health foods It causes the so-called rebound phenomenon of weight gain.

Recently, a diet health food containing a Garcinia extract containing hydroxycitric acid has been marketed. Garcinia extract works to suppress fat synthesis in the body and reduce appetite. Therefore, a diet health food containing this Garcinia extract,
It has an effect of suppressing weight gain and improving obesity. However, this diet health food does not directly act on fat that has already accumulated. Therefore,
At present, there is no dietary health food that directly acts on already accumulated fat and promotes its decomposition to improve obesity.

[0006]

DISCLOSURE OF THE INVENTION The present invention solves these conventional problems and has the ability to directly act on fat already accumulated in the body to promote its decomposition, thereby improving obesity. An object of the present invention is to provide a composition for promoting lipolysis which can achieve suppression of weight gain and has no problem in terms of safety.

[0007]

Means for Solving the Problems The present inventors conducted screening using a cultured cell system in order to search for a substance that promotes the decomposition of fat contained in fat cells. That is, after the 3T3-L1 cells, which are preadipocytes, are differentiated into adipocytes, various plant extracts are added thereto, and lipolysis is promoted by a method of measuring the amount of glycerol released by lipolysis. Material screening was performed. As a result, among various plant extracts, it has been found that skin extracts and their related citrus peel extracts, which have been used only for the purpose of stomachic and antitussive, have a remarkable lipolysis promoting effect. . In addition, the present inventors have confirmed that when a citrus peel extract having a lipolysis-promoting action is consumed by humans, weight gain is actually suppressed and obesity is also improved. The present invention has been made based on these findings.

[0008] That is, the present invention provides a composition for accelerating lipolysis, comprising a water extract or a low-boiling organic solvent extract of citrus peel as a main component.

[0009]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail.
As the citrus peel used as a raw material of the composition of the present invention, any citrus peel such as cinnamon skin (orange peel), orange peel (daidai peel), hassaku skin and buntan skin can be used. Among them, it is particularly preferable to use skin. Chen skin, etc., as described above, has been conventionally used as a crude drug in Chinese medicine and the like for the purpose of digesting stomachic and removing antitussives.
Not known at all.

The composition for accelerating lipolysis of the present invention comprises a water extract or a low-boiling organic solvent extract of citrus peel as a main component as described above. Can be manufactured.

That is, hot water is added to the dried citrus peel to extract it by heating, or a low-boiling organic solvent is added thereto, followed by extraction by stirring, and then, if necessary, a hydraulic press machine or the like. After squeezing, and further removing the insolubles by means such as centrifugation and filter filtration, the filtrate is concentrated to about Bx10 to 20 and then the solution is freeze-dried or spray-dried, for example. Thereby, a powdery extract can be obtained.

More specifically, it is as follows. First, a dried citrus peel, such as skin, is prepared. Then, about 15 to 20 times, preferably about 20 times the amount of hot water is added to the citrus peel 1 and heated for about 1 to 3 hours. Here, the temperature of the hot water is 55 ° C. or higher,
Preferably, it is 60 ° C. or higher. Further, instead of adding hot water and heating, a low boiling point organic solvent of about 15 to 20 times, preferably about 20 times the amount of citrus peel 1 is added,
Stirring may be performed for about 1 to 3 hours. As the low-boiling organic solvent, an organic solvent having a boiling point of about 50 to 65 ° C, such as ethanol, can be used.

Next, if necessary, the juice is squeezed using a hydraulic press or the like, and the insolubles are removed by means of centrifugation, filtration with a filter, or the like. Concentrate to a degree. Then, by freeze-drying or spray-drying this solution,
An extract powder can be obtained. In addition, an excipient can also be produced by adding an excipient such as dextrin to the concentrated solution before drying and then drying. In this case, dextrin or the like is added to the concentrated solution concentrated to about Bx10 (solid content about 10%) to obtain Bx20.
Concentration (about 20% solids) and freeze-drying or spray-drying this.

The extract thus obtained may contain, if necessary, crude drug components, vitamins, stabilizers, preservatives,
Conventional components such as antioxidants, sugars, coloring agents, flavors, and fruit juices can be mixed.

In the present invention, the powder obtained by mixing the above-mentioned optional components can be used as it is as a powder, or it can be made into a tablet by adding an excipient, or can be made into water. It can be used in various forms, such as being dissolved into a drink. Furthermore, if necessary, it can be contained in foods.

The intake amount of the composition for promoting lipolysis of the present invention thus obtained should basically be an amount effective for promoting lipolysis in adipocytes. For example, in the case of a tablet form, 150
What is necessary is to take about 5 to 15 tablets a day containing about mg. The lipolysis effect is observed immediately after taking the drug, but becomes noticeable from about 20 days. Therefore, the patient should continue taking the drug for at least about 20 days. When it is contained in foods, the content is about several percent to 30%, preferably 10% or more.

Since the composition of the present invention thus obtained has a lipolysis promoting effect, it can be used as an effective diet health food material. A diet health food comprising the composition of the present invention is a new type of health food that suppresses weight gain by promoting lipolysis and improves obesity, instead of simply suppressing calorie intake as in conventional diet food compositions. Functional food).

[0018]

EXAMPLES Next, the present invention will be described in more detail with reference to examples, but the scope of the present invention is not limited by these examples.

Production Example 1 [Manufacture of Chen Skin Extract Powder] Chen Skin Powder (trade name: Chezami Powder, Tokyo Koshin Co., Ltd.)
200 l of water for 10 kg, and
Time-warmed extraction was performed. Thereafter, the juice was squeezed using a hydraulic press (trade name: KS-3, manufactured by Komagata Kikai Seisakusho), and the insoluble matter was removed by centrifugation and filtration with a filter. The obtained filtrate was subjected to Bx1
It was concentrated to 0. 3.5 kg of dextrin was added to this concentrated liquid (about 35 L) to obtain Bx20. This solution was spray-dried under the conditions of an inlet temperature of 180 ° C. and an outlet temperature of 80 ° C. to obtain 7.0 kg of a skin extract.

Manufacturing Example 2 [Manufacture of tablet containing powdered skin extract] 900 g of the powdered skin extract prepared in Production Example 1, 150 g of dietary fiber, 12 g of vitamins, 618 g of saccharides and 1320 g of tableting excipient were compounded and tableted. And 500 mg x 60
00 triangular tablets were obtained.

Production Example 3 [Production of orange peel extract] In Production Example 1, except that orange peel powder (trade name: Spruce powder, manufactured by Nippon Powder Chemical Co., Ltd.) was used instead of Chen powder powder. Performed in the same manner as in Production Example 1, and dried orange peel extract 7.0 kg
I got

Comparative Production Example 1 A triangular tablet was produced in the same manner as in Production Example 2, except that the dermis extract powder was replaced with dextrin.

Test Example 1 [Measurement of Lipolysis Acceleration Action in Adipocytes] The skin extract extract prepared in Preparation Example 1 and the orange peel extract prepared in Preparation Example 2 were examined for their lipolysis promotion action. The amount of glycerol generated by lipolysis was measured. 3T3, a mouse-derived preadipocyte
The -L1 cells were added to a 1.0 × 10 ⁴ / well in 96-well plates, 20% FCS Dulbecco's -MEM
The cells were cultured in a culture solution at 37 ° C. in the presence of 5% CO ₂ .
Immediately before the cells became confluent, 1-methyl-3-isobutylxanthine, dexamethasone and insulin were added to the culture to induce differentiation into adipocytes. 1 to
After culturing for 2 weeks, when differentiation into adipocytes was confirmed, the skin extract or orange peel extract at the concentrations shown in Table 1 was added to the culture solution and incubated overnight. After the incubation, each culture was collected and the amount of glycerol released, which is a decomposition product of fat released in the culture, was measured. The results are shown in Table 1. Table 1 shows some of the screening results of plant extracts other than Chen-sin extract powder prepared in Production Example 1 and orange peel extract powder prepared in Production Example 2.

The amount of glycerol released in Table 1 is as follows:
This is a relative value when the amount of glycerol released when nothing is added to the medium is 100%.

[0025]

[Table 1]

Table 1 shows the following. When citrus-based skin extract extract or orange peel extract is added, it can be confirmed that a larger amount of glycerol is liberated than in extracts of other plant species, particularly when the amount added to the medium is 0.005% or more. In addition, in particular, in the case of Chen extract, the amount of glycerol released increases in proportion to the increase in the concentration added to the medium. From this, it can be seen that the skin extract and orange peel extract comprising the composition of the present invention have the ability to decompose fat in fat cells.

Test Example 2 [Inhibition of Weight Gain on Rats] Wistar female rats (7 weeks old, 24 rats) were divided into 3 groups of 8 rats each. The first group is shown in Table 2 below. A bait comprising the basic bait composed of the composition and the skin extract extract 4% prepared in Production Example 1 was added to the bait (a group to which the skin bar was added: the present invention section), and the second group was fed only the basal bait and raised. Basic bait group: control group). Further, the third group was fed and fed with a commercially available Garcinia extract-added diet (Garcinia extract-added group). The basic bait is a high calorie fat-forming food, and its composition is as shown in Table 2.

[0028]

[Table 2]

[0029] In each section, food was freely taken and bred for 20 days. For each individual, the body weight was measured before the start of the test and after the end of the test, and the average weight gain in each group and the inhibition rate of the group of the present invention (the skin-added group) were calculated. The results are shown in Table 3.

[0030]

[Table 3] *: P <0.05

From Table 3, it can be seen that the weight gain of the rats in the group of the present invention (the group with added skin) was significantly lower than that of the rats in the control group (the basic diet group). From this, it was confirmed that the skin extract comprising the composition of the present invention had an effect of suppressing weight gain in rats. From Table 3, it can be seen that Garcinia extract also has an effect of suppressing weight gain in rats, but it is known that this Garcinia extract does not directly act on fat that has already accumulated. .

Test Example 3 [Effect on Humans] Five volunteers took 10 tablets of the skin extract extract prepared in Production Example 2 per day. As a control, five volunteers similarly took the tablet prepared in Comparative Production Example 1. The test was performed for one month. The body weight was measured before the start of the test and after the end of the test, and the amount of change in body weight for one month (average value of five subjects)
Was calculated. The results are shown in Table 4.

[0033]

[Table 4] *: P <0.01

As can be seen from Table 3, a slight increase in body weight was observed in the control group in which the tablet of Comparative Production Example 1 was taken, but in the group of the present invention in which the tablet of Production Example 2 was taken, the weight was about 1.6 kg.
Weight loss was seen. From this, it was clarified that the tablet containing the skin extract comprising the composition of the present invention also has a weight-reducing effect on humans.
Further, the tablet containing the skin extract powder comprising the composition of the present invention acts directly on fat that has already accumulated, has the ability to promote its decomposition, and achieves an improvement in obesity. You can see that it can be done.

[0035]

The composition for accelerating lipolysis of the present invention has the ability to decompose fat contained in fat cells, and directly acts on fat already accumulated in the body to promote the decomposition. . Therefore, if a diet health food is produced by using the composition of the present invention as a main component, it is possible to achieve improvement of obesity or suppression of weight gain by eating it. In addition, citrus peels such as rind have been used as Chinese herbs for a long time, so that the composition for accelerating lipolysis of the present invention has very high safety. Therefore, the composition for promoting lipolysis of the present invention can be used as a diet health food of a new type which is different from the way of acting of the conventional diet health food and which is highly safe.

Claims (1)

[Claims] 1. A composition for promoting lipolysis comprising a water extract or a low-boiling organic solvent extract of citrus peel as a main component.