JPH1017310A - Collagen, production of hydroxyapatite and its product - Google Patents

Abstract

PROBLEM TO BE SOLVED: To establish the process for production of hydroxyapatite and non- denatured collagen and to effectively utilize the products by using bones easily available as the by-products of meat industry as a starting raw material. SOLUTION: The feet of broilers finely crushed by a crushing machine are subjected to a pretreatment by a method for washing these legs with water and <=0.5N aq. sodium chloride soln., then immersing the feet into 0.1N aq. sodium hydroxide soln. The pretreated raw materials are then immersed into acetic acids of various concns. and the immersing liquid is neutralized and the precipitate is subjected to freeze drying, by which the hydroxyapatite and the fibrous non-denatured collagen are obtd. from the bones.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing collagen and hydroxyapatite using bone as a starting material, and to an effective use of unmodified collagen and hydroxyapatite obtained by the production method.

[0002]

2. Description of the Related Art Chicken feet, which are discharged in large quantities as the poultry (broiler) business becomes larger, are used as raw materials for instant noodle soup bird gala and broiler feed, except that some are exported as ingredients. Only used as.
Collagen constituting bone is only extracted as gelatin, and only bovine and pig skins are used for collagen production.

[0003]

SUMMARY OF THE INVENTION An object of the present invention is to extract collagen and hydroxyapatite from bone, which can be easily obtained as a by-product of the meat industry, and to effectively use the by-product of the meat industry.

[0004]

In order to achieve the above-mentioned object, the method of the present invention comprises the steps of crushing the skin or legs of a chicken or the like with a crusher using a crusher, and adding sodium chloride and sodium hydroxide. After pretreatment with an aqueous solution, immersion in acetic acid of various concentrations, lyophilization of the precipitate obtained by neutralizing the immersion liquid, to obtain hydroxyapatite and fibrous unmodified collagen from bone. is there.

[0005]

According to the above-mentioned production method, the sodium chloride and sodium hydroxide used in the pretreatment act on blood, lipids and proteins adhering to bones and skins to extract collagen and hydroxyapatite. Impurities can be removed.

Next, in order to extract collagen and hydroxyapatite individually, the type and concentration of acid were examined. As a result, acetic acid was most suitable for efficient extraction of inexpensive acids without denaturing collagen. By using acetic acid at a concentration lower than 0.5 N, only hydroxyapatite could be eluted. After the hydroxyapatite was eluted, it was possible to extract only collagen by increasing the concentration of acetic acid.

The obtained hydroxyapatite was smokeless and odorless even when heated at 900 ° C. for 1 hour, and had a smaller weight loss rate than bone ash produced by a known method. Bone ash porcelain made from bone ash produced by this method had higher whiteness than commercially available ones.

[Table 1]

[Table 2]

[0008] From an experiment using mice, calcium absorption into bone was higher than that of the conventional one.

Undenatured collagen can be obtained in a fibrous form, and can be used for paper mixed with pulp.
The oil absorption of the paper-mixed paper was improved as the collagen content increased.

FIG.

[0010]

Example 1 The present invention will be described more specifically by the following examples, but the present invention is not limited to these examples.

The broiler's feet are crushed with a crusher, washed with water, washed three or more times with an aqueous solution of sodium chloride having a concentration of 0.5 N or less, and then suspended in an aqueous solution of 0.1 N sodium hydroxide. Stir gently for at least an hour.

The solid is then suspended in 0.1 N acetic acid and
Leave for more than an hour. This substance was filtered with a 5A filter paper, and the liquid phase was neutralized with sodium hydroxide to precipitate hydroxyapatite, which was recovered after washing with distilled water.

The extraction residue was extracted again with 0.1N acetic acid, and the operation was repeated until hydroxyapatite was completely separated.

Next, the extraction residue of hydroxyapatite was reduced to 0.
It was suspended in acetic acid of 5N or more and left for 24 hours or more. Filter this material through a 5A filter paper, collect the liquid phase, collect the solid,
Sodium hydroxide was added to the liquid phase to precipitate collagen, which was quantitatively transferred to a 36/32 dialysis membrane and dialyzed during distillation.

The collagen was spread on a bat and freeze-dried to obtain fibrous collagen.

[0016]

Example 2 The precipitate obtained in Example 1 was freeze-dried to obtain powdery hydroxyapatite. This at 900 ° C
Heat for a time to make bone ash, bone ash, sericite clay, feldspar,
Kaolin was blended at a weight ratio of 4: 3: 2: 1 and wet mixed and ground by a pot mill to obtain a green body. After molding, 1200
By firing in an oxidizing flame atmosphere at ℃, a bone porcelain body was obtained.

[Brief description of the drawings]

BRIEF DESCRIPTION OF DRAWINGS FIG. 1 is a graph showing oil absorbency according to a collagen blending ratio of a blended paper in which unmodified collagen is made.

Claims (5)

[Claims] 1. A method for producing hydroxyapatite from bone, comprising the steps of (a) eluting hydroxyapatite with dilute acetic acid, (b) neutralizing the eluate with an alkaline solution to precipitate hydroxyapatite, (c) ) A method for producing hydroxyapatite, comprising the step of freeze-drying the precipitate to obtain powdered hydroxyapatite. 2. The method for producing native collagen from the residue according to claim 1 (a), wherein (a) extracting collagen with acetic acid or dilute acetic acid, and (b) neutralizing the extract with an alkaline solution. And c) freeze-drying the precipitate to obtain fibrous collagen. 3. The method according to claim 1, wherein chicken feet are used as a starting material. 4. Use of hydroxyapatite produced by the method according to claim 1 for foodstuffs for bone porcelain or calcium supplementation. 5. Use of collagen produced by the method according to claim 2 for paper or cosmetics.