JPH0943223A - Capillary column - Google Patents
Capillary columnInfo
- Publication number
- JPH0943223A JPH0943223A JP19213695A JP19213695A JPH0943223A JP H0943223 A JPH0943223 A JP H0943223A JP 19213695 A JP19213695 A JP 19213695A JP 19213695 A JP19213695 A JP 19213695A JP H0943223 A JPH0943223 A JP H0943223A
- Authority
- JP
- Japan
- Prior art keywords
- capillary column
- column
- inner diameter
- capillary
- stationary phase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6034—Construction of the column joining multiple columns
- G01N30/6043—Construction of the column joining multiple columns in parallel
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/466—Flow patterns using more than one column with separation columns in parallel
- G01N30/467—Flow patterns using more than one column with separation columns in parallel all columns being identical
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、クロマトグラフ分
析装置に使用するキャピラリカラムに関する。TECHNICAL FIELD The present invention relates to a capillary column used in a chromatographic analyzer.
【０００２】[0002]
【従来の技術】クロマトグラフ装置に用いるキャピラリ
カラムは、溶融石英、ガラス、ステンレスなどの材料を
用いて開管チューブを形成し、その内壁に固定相を結合
するようにして作られている。2. Description of the Related Art A capillary column used in a chromatographic apparatus is made by forming an open tube using a material such as fused quartz, glass or stainless steel, and binding a stationary phase to the inner wall of the tube.
【０００３】一般に高速、高分離のクロマト分析を行う
ときは内径が小さく、固定相膜厚の薄いキャピラリカラ
ム（たとえば図２に示す内径０．１ｍｍのもの）が用い
られる。また、これとは逆に、多量のサンプルを導入し
て分析を行うときは内径が大きく固定相膜厚が厚いキャ
ピラリカラム（たとえば図３に示す内径０．４ｍｍのも
の）が用いられる。Generally, when performing high-speed, high-resolution chromatographic analysis, a capillary column having a small inner diameter and a small stationary phase film thickness (for example, an inner diameter of 0.1 mm shown in FIG. 2) is used. On the contrary, when a large amount of sample is introduced for analysis, a capillary column having a large inner diameter and a large stationary phase film thickness (for example, an inner diameter of 0.4 mm shown in FIG. 3) is used.
【０００４】[0004]
【発明が解決しようとする課題】高速高分離分析を行う
ために、内径が小さい膜厚の薄いカラムを使用すると、
カラムに導入できるサンプル量が少なくなり、分析の感
度が低下する。一方、感度を高くするために、内径が大
きく固定相膜厚が厚いカラムを使用すると、分離性能が
悪くなる。When a thin column having a small inner diameter is used to perform high-speed and high-resolution analysis,
The amount of sample that can be introduced into the column decreases, and the sensitivity of the analysis decreases. On the other hand, if a column having a large inner diameter and a large stationary phase film thickness is used in order to increase the sensitivity, the separation performance deteriorates.
【０００５】表１は代表的なカラム内径のキャピラリカ
ラムを例にスピード、分離効率、サンプル負荷量の比
（内径０．２５ｍｍを１としたときの相対比）を示すも
のであるが、このように高速分離性能と、感度とは互い
に相反する関係にあるため、高速分離性能と感度との両
特性を同時に満足することはできなかった。Table 1 shows the ratio of the speed, the separation efficiency, and the sample load amount (relative ratio when the inner diameter of 0.25 mm is 1) using a capillary column with a typical column inner diameter as an example. Since the high-speed separation performance and the sensitivity are contradictory to each other, it was not possible to satisfy both the high-speed separation performance and the sensitivity at the same time.
【０００６】本発明は以上のような問題を解決し、高速
高分離でかつ感度の高い分析を行うのに好適なカラムを
提供することを目的とする。An object of the present invention is to solve the above problems and to provide a column suitable for high-speed, high-resolution and highly sensitive analysis.
【０００７】[0007]
【表１】 [Table 1]
【０００８】[0008]
【課題を解決するための手段】上記問題を解決するため
になされた本発明のキャピラリカラムは、内径および固
定相膜厚が均一である複数の管状孔を有するキャピラリ
カラムであることを特徴とする。The capillary column of the present invention, which has been made to solve the above problems, is characterized in that it is a capillary column having a plurality of tubular holes having a uniform inner diameter and stationary phase film thickness. .
【０００９】[0009]
【実施の形態】本発明のキャピラリカラムでは、その内
径と同じ内径を有する単一孔のキャピラリカラムと同じ
分離効率を持ち、さらにその開管部の総断面積（総固定
相膜厚）と同じ断面積（固定相膜厚）のカラムを用いた
ときの同じサンプル量をカラムに導入することができ
る。BEST MODE FOR CARRYING OUT THE INVENTION The capillary column of the present invention has the same separation efficiency as a single-hole capillary column having the same inner diameter as that of the inner diameter, and has the same total cross-sectional area (total stationary phase film thickness) of the open tube portion. The same sample amount when using a column of cross-sectional area (stationary phase film thickness) can be introduced into the column.
【００１０】以下、本発明の実施例を図を用いて説明す
る。図１は本発明の一実施例を示すキャピラリカラムで
ある。このキャピラリカラムは内径１．０ｍｍ程度のカ
ラム本体１０に内径０．１ｍｍの１６本の管状孔が形成
されているものである。各管状孔２０の内壁面には被測
定物質に適した固定相液体が均一にコーティングされて
いる。Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a capillary column showing an embodiment of the present invention. This capillary column has a column body 10 having an inner diameter of about 1.0 mm and 16 tubular holes having an inner diameter of 0.1 mm formed therein. The inner wall surface of each tubular hole 20 is uniformly coated with a stationary phase liquid suitable for the substance to be measured.
【００１１】このようなキャピラリカラムを使用するこ
とにより、内径が０．１ｍｍのカラムと同程度のスピー
ドと分離効率を有し、さらに、内径が０．１ｍｍのカラ
ムを１６本束ねたと同程度のサンプル負荷量にすること
ができる。By using such a capillary column, the same speed and separation efficiency as those of the column having the inner diameter of 0.1 mm can be obtained, and further, it is the same as that of bundling 16 columns having the inner diameter of 0.1 mm. Can be sample loading.
【００１２】すなわち、スピード、分離効率、サンプル
負荷量のすべてについて優れた特性を持つカラムを実現
することができる。That is, it is possible to realize a column having excellent characteristics in terms of speed, separation efficiency, and sample loading amount.
【００１３】本実施例ではカラム本体１０に管状孔を開
けた構成としたが、図４に示すように、０．１ｍｍのキ
ャピラリカラム３１を用いてこれを多数本束ねるととも
に、外筒３３に入れ、各キャピラリカラム間をガラス３
２で封入するようにして一体とすることで、同様の構成
とすることができる。この実施態様を以下にまとめてお
く。 （１）内径および固定相膜厚が均一な細径のキャピラリ
カラムを複数本束ねて外筒内に入れ、前記外筒内にて各
キャピラリカラム間を封入部材により埋め込んだことを
特徴とするキャピラリカラム。In this embodiment, the column main body 10 has a tubular hole. However, as shown in FIG. 4, many 0.1 mm capillary columns 31 are bundled together and placed in the outer cylinder 33. , Glass 3 between each capillary column
The same configuration can be obtained by integrating the two by enclosing them in two. This embodiment is summarized below. (1) Capillary characterized in that a plurality of small-diameter capillary columns having a uniform inner diameter and a fixed phase film thickness are bundled and placed in an outer cylinder, and a space between the respective capillary columns is filled with a sealing member in the outer cylinder. column.
【００１４】[0014]
【発明の効果】以上説明したように本発明のキャピラリ
カラムは、上記のような構成としたので、その内径と同
じ内径を有する単一孔のキャピラリカラムと同じ分離効
率を持ち、さらにその開管部の総断面積（総固定相膜
厚）と同じ断面積（固定相膜厚）のカラムを用いたとき
の同じサンプル量をカラムに導入することができる。As described above, since the capillary column of the present invention has the above-mentioned structure, it has the same separation efficiency as that of the single-hole capillary column having the same inner diameter as that of the inner diameter of the capillary column. The same sample amount when using a column having the same cross-sectional area (stationary phase film thickness) as the total cross-sectional area (total stationary phase film thickness) of the part can be introduced into the column.
【００１５】したがって、従来の細径キャピラリカラム
を使用したときは流路抵抗が大きいため、高いカラム入
口圧をかける必要があり、そのために、大量のキャリア
ガスを導入し捨てなければならなかったが、本発明のカ
ラムを用いることにより、入口圧を低くすることがで
き、スプリット比も小さくすることができる。Therefore, when the conventional small-diameter capillary column is used, since the flow path resistance is large, it is necessary to apply a high column inlet pressure. Therefore, a large amount of carrier gas must be introduced and discarded. By using the column of the present invention, the inlet pressure can be lowered and the split ratio can be reduced.
【図１】本発明の一実施例であるキャピラリカラムを示
す図。FIG. 1 is a view showing a capillary column which is an embodiment of the present invention.
【図２】従来からのキャピラリカラムを示す図。FIG. 2 is a view showing a conventional capillary column.
【図３】従来からのキャピラリカラムを示す図。FIG. 3 is a view showing a conventional capillary column.
【図４】本発明の他の一実施例であるキャピラリカラム
を示す図。FIG. 4 is a view showing a capillary column which is another embodiment of the present invention.
１０：カラム本体 ２０：管状孔 ２１：固定相膜 ３１：キャピラリカラム ３２：ガラス ３３：外筒 10: Column body 20: Tubular hole 21: Stationary phase membrane 31: Capillary column 32: Glass 33: Outer cylinder
Claims (1)
管状孔を有するキャピラリカラム。1. A capillary column having a plurality of tubular holes having a uniform inner diameter and a stationary phase film thickness.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19213695A JPH0943223A (en) | 1995-07-27 | 1995-07-27 | Capillary column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19213695A JPH0943223A (en) | 1995-07-27 | 1995-07-27 | Capillary column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0943223A true JPH0943223A (en) | 1997-02-14 |
Family
ID=16286290
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP19213695A Pending JPH0943223A (en) | 1995-07-27 | 1995-07-27 | Capillary column |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0943223A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0926492A1 (en) * | 1997-12-02 | 1999-06-30 | Uop Llc | Round profile multi-capillary assembly useful in chromatography |
| US6174352B1 (en) | 1998-11-24 | 2001-01-16 | Uop Llc | Round profile multi-capillary assembly and method of making |
| JP2005233941A (en) * | 2004-01-23 | 2005-09-02 | Ngk Insulators Ltd | Carrier for solid phase extraction |
| JP2014119430A (en) * | 2012-12-19 | 2014-06-30 | Frontier Lab Kk | Capillary column |
| CN113769438A (en) * | 2021-10-15 | 2021-12-10 | 温州大学 | A large-diameter chromatographic stationary phase with a double monolithic structure and its in-situ preparation method |
-
1995
- 1995-07-27 JP JP19213695A patent/JPH0943223A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0926492A1 (en) * | 1997-12-02 | 1999-06-30 | Uop Llc | Round profile multi-capillary assembly useful in chromatography |
| US6174352B1 (en) | 1998-11-24 | 2001-01-16 | Uop Llc | Round profile multi-capillary assembly and method of making |
| JP2005233941A (en) * | 2004-01-23 | 2005-09-02 | Ngk Insulators Ltd | Carrier for solid phase extraction |
| JP2014119430A (en) * | 2012-12-19 | 2014-06-30 | Frontier Lab Kk | Capillary column |
| CN113769438A (en) * | 2021-10-15 | 2021-12-10 | 温州大学 | A large-diameter chromatographic stationary phase with a double monolithic structure and its in-situ preparation method |
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