JPH09241163A - Beverage and composition containing barbaloin and production thereof - Google Patents

Abstract

PROBLEM TO BE SOLVED: To produce a composition, comprising ascorbic acids and barbaloin and capable of preserving the barbaloin in a state of an aqueous solution for a long period. SOLUTION: This composition comprises one or more of compounds (ascorbic acids) selected from L-ascorbic acid and an alkali metallic salt, a fatty acid ester, phosphoric ester or a saccharide transfer product (e.g. a glucose transfer product) thereof and barbaloin. A juice or an extract solution obtained from a leaf part in an aloe of a plant belonging to the genus Aloe of the family Liliaceae containing the barbaloin or other terrestrial parts by pressing of the juice, leaching, extraction, etc., and a concentrate, a purified substance, etc., are used as the barbaloin. The extraction is carried out by using a hydrous ethanol at normal temperatures or under refluxing and heating. The barbaloin is stabilized by blending with the ascorbic acids, but more stabilized by the coexistence of the ethanol therewith. Laxative actions of the barbaloin in the form of a readily ingestible substance, beverage, etc., can be utilized.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a composition containing barvaloin and a method for producing the same.

[0002]

2. Description of the Related Art Barvaloin is a kind of anthrone glycoside having the structure of the following chemical formula 1, and it has been confirmed that it has a laxative effect.

[0003]

Embedded image

Barbaroin is naturally contained in many aloe plants belonging to the family Liliaceae (eg Aloe ferox, Aloe a).
fricana, Aloe spicata, Aloe arborescens, etc.), aloe juice containing these barvaloins or concentrated dried product thereof is used as a medicine or a folk medicine on a small scale. However, since barvaloin separated from aloe is very unstable in an aqueous solution state, it is necessary to use fresh juice as much as possible if the above pharmacological action of barvaloin is expected. In addition, it was impossible to use a liquid formulation as a storable pharmaceutical product, and it was necessary to make it a solid formulation. Needless to say, it was not possible to provide it in the form of a drink that is easy to take on a daily basis.

It has not been confirmed why barbaroin is unstable in an aqueous solution state, and no method for improving the stability of barvaloin in water has been known.

[0006]

The object of the present invention is to find means for keeping barvaloin stable even in an aqueous solution state,
It is to improve the utilization efficiency when barbaroin is extracted from aloe or the like and used.

Another object of the present invention is to provide a composition containing barvaloin, which is stable even in an aqueous solution, and facilitates utilization of the pharmacological action of barvaloin.

[0008] Still another object of the present invention is to provide a beverage containing barvaloin which is stable for a long period of time and facilitates daily intake of barvaloin.

[0009]

The present invention, which has succeeded in attaining the above-mentioned object, consists of the following respective inventions. One or more compounds (hereinafter referred to as ascorbic acids) selected from the group consisting of L-ascorbic acid and its alkali metal salts, fatty acid esters, phosphoric acid esters, or sugar transfer products (eg glucose transfer products) coexist with barvaloin. The composition obtained.

A composition comprising one or more ascorbic acids and barvaloin coexisting in hydrous ethanol.

A beverage containing at least one ascorbic acid together with barvaloin.

A method for producing the above composition, which comprises subjecting aloe containing barbaroin to extraction treatment with hydrous ethanol, and mixing one or more ascorbic acids with the obtained extract or a concentrate thereof.

A method for producing the above composition, which comprises subjecting aloe containing barvaloin to an extraction treatment with hydrous ethanol containing one or more ascorbic acids, and concentrating or drying the obtained extract. .

That is, the present invention is based on the novel finding by the present inventors that barbaroin in an aqueous solution is remarkably stabilized by ascorbic acids.

[0015]

BEST MODE FOR CARRYING OUT THE INVENTION As barvaloin used in the present invention, juice or extract obtained by operations such as squeezing, leaching, and extracting from a leaf part or other above-ground part of aloe containing barvaloin, and those The concentrate, purified product, etc. of can be used. As is well known, aloe contains many useful ingredients other than barvaloin and is often used as a raw material for health drinks, so depending on the application (for example, when it is used as a drink) The aloe extract containing barbaroin at a low concentration can be used as it is in the composition of the present invention (specifically, a beverage or the like).

The aloe used as the barbaroin extraction raw material may be either fresh or dried. Barbaroin is stable while it is in Aloe cells, but immediately after it is eluted, it seems to undergo some action to decompose or inactivate it. Therefore, it is desirable that the operation of extracting barbaroin does not change barbaroin during extraction. As a result of various studies, it was found that the extraction with hydrous ethanol is preferable because the loss of barvaloin is less than that by extraction by squeezing or leaching, and that by extraction with hot water.

As the hydrous ethanol used for extraction, those having an ethanol concentration of about 20 to 95 vol% are suitable. The extraction can be performed at room temperature or under heating with reflux. The obtained extract is concentrated to a solid content concentration suitable for the intended use of the product, and further dried if necessary.

At any stage up to this point, ascorbic acid is mixed with the extracted barbaroin to stabilize barbaroin. That is, ascorbic acid is added to the extraction solvent at the stage of the extraction operation. Ascorbic acid is immediately added to the obtained extract. Ascorbic acid is mixed with the concentrate or dried product of the extract. As described above, ascorbic acid is allowed to coexist with barvaloin to obtain the composition of the present invention.

Ascorbic acid to be added is most preferably ascorbic acid which is widely used as vitamin C. A fruit juice concentrate having a high content of ascorbic acid, for example, a juice concentrate of acerola rosehip, prickly pear, citrus fruits or the like can be used instead of ascorbic acid.

Stabilization of barvaloin by ascorbic acid is ascorbic acid even in a trace amount (eg, about 0.2% by weight; see Example 4 below) in a system in which barvaloin is dissolved regardless of the concentration of barbaroin. It will be achieved. However, when added to beverages, etc., ascorbic acid also gradually decomposes under the influence of oxygen, heat, light, etc., so an excessive amount of ascorbic acid may be added depending on the intended use of the composition and the expected shelf life. It is desirable to add acids.

Barvaloin is inherently unstable even in the extract concentrate. Ascorbic acid stabilizes it, but when ethanol coexists there, barvaloin becomes more stable. Therefore, it is preferable for improving the stability of barvaloin to add a small amount of ethanol to the concentrate, which is in the state of aqueous solution by evaporation of ethanol during concentration, to improve the stability of barvaloin.

The barbaroin-containing composition of the present invention obtained as described above is an aqueous solution (from the extract to the concentrate).
It can be stored even in the state of without being affected by the change of barvaloin (the dried product is stable in the state without expecting the stabilizing action of ascorbic acid, but the aqueous solution was used. Occasionally, the ascorbic acid-induced stabilizing effect of barvaloin appears.) Since the components coexisting with barbaroin are harmless aloe components and ascorbic acids other than barbaroin, liquid, drink, tincture, etc. When trying to utilize, barbaroin can be used as it is as a raw material without isolation.

The composition of the present invention containing barbaroin is most suitable as a raw material for producing a barbaroin-containing beverage. That is, a beverage containing barbaroin stabilized by ascorbic acids is obtained by simply dissolving the composition of the present invention at any stage of the process for producing a beverage having any composition. The ascorbic acid that enters the beverage together with barbaroin is a component added to many beverages as vitamin C or as an acidulant, so that it has no inconvenience and can be effectively used.

Needless to say, aloe extract containing barbaroin or its concentrate and ascorbic acid are added separately (that is, without passing through the composition of the present invention) in the beverage production process, Stabilization of barvaloin in beverages with ascorbic acids is achieved.

The concentration of barvaloin in the beverage is not particularly limited, but is preferably about 0.05% by weight or less. When it is contained at a higher concentration than that, it is not difficult to stabilize it with ascorbic acid, but the dissolved state becomes unstable, and a precipitate is likely to occur.

Specific examples of the beverage which can stably contain barvaloin according to the present invention include various soft drinks (including so-called nutritional drinks, sports drinks and the like), fruit drinks, vegetable juices, milk drinks and the like.

Barbaroin contained in the aloe extract and the composition of the present invention can be quantified by high performance liquid chromatography. The quantitative value of barvaloin described in this specification is based on the following measurement conditions. Column: Wakosil II 5C18-HG 4.6 x 250
mm (Wako Pure Chemical Industries, Ltd.) Mobile phase: acetonitrile / 0.1% phosphoric acid (22/7
8) Detection: UV-293nm Flow rate: 1.0 ml / min Column temperature: 40 ° C

[0028]

【Example】

Aloe Extract Production Example 1 10 kg of 30% ethanol was added to 1 kg of dried leaves of Pleurotus cornucopiae, and heat extraction was performed for 1 hour under reflux. After cooling, the extract obtained by filtration was concentrated under reduced pressure to 4 kg. To the obtained concentrated liquid, 1 liter of ethanol was added, the insoluble matter formed was removed by filtration, concentrated under reduced pressure to 800 ml, and finally 200 ml of ethanol was added. The obtained aloe extract contained 0.2% by weight of barvaloin.

Aloe Extract Production Example 2 10 g of 50% ethanol was added to 500 g of dried leaves of Cape aloe, and heat extraction was carried out for 1 hour under reflux. After cooling, the extract obtained by filtration was concentrated under reduced pressure to 2 kg. 500 ml of ethanol was added to the obtained concentrated liquid, the resulting insoluble matter was removed by filtration, the mixture was concentrated under reduced pressure to 200 ml, and finally 50 ml of ethanol was added. The obtained aloe extract contained 0.3% by weight of barvaloin.

Examples 1 to 4 Beverage containing 55% fructose-glucose liquid sugar 18.2 g, citric acid monohydrate 0.1 g, and perfume 0.1 g per 100 g, as a basic component, aloe extract 4 containing aloe extract and ascorbic acid according to Production Example 1
A variety of beverages (Examples 1-4) were produced.

For comparison, beverages containing barbaroin (Comparative Examples 1 to 3) were produced in the same manner as in Examples 1 to 3 except that ascorbic acid was not added. The aloe extract content and ascorbic acid content of each example beverage are as follows.

[0032]

Table 1 Aloe extract (g) Ascorbic acid (g) Example 1 25.0 0.5 Comparative Example 1 25.0 0 Example 2 1.0 0.5 Comparative Example 2 1.0 0 Example 3 0 .05 0.5 Comparative Example 3 0.05 0 Example 4 1.0 0.2

Each of the obtained beverages was immediately placed in a brown bottle, sealed tightly, and stored in a thermostat at 40 ° C. for 25 days. Table 2 shows the results of examining the change over time in the barvaloin content in the above storage test.

[0034]

[Table 2] Time-dependent change in barvaloin content (% by weight) Immediately after production 3 days 9 days 18 days 25 days Example 1 0.050 0.049 0.050 0.049 0.050 Comparative Example 1 0.050 0.032 0.000 0.000 0.000 Example 2 0.0020 0.0020 0.0019 0.0019 0.0020 Comparative Example 2 0.0020 0.0013 0.000 0.000 0.000 Example 3 0.00010 0.00011 0.00010 0.00009 0.00010 Comparative Example 3 0.00010 0.00005 0.00010 0.00009 0.00010 Example 4 0.0020 0.0020 0.0019 0.0019 0.0011

Example 5 10 kg of water was added to 1 kg of dried leaves of Pleurotus cornucopiae and heat extraction was carried out under reflux for 1 hour. After cooling, the extract obtained by filtration was concentrated under reduced pressure to 4 kg. 300 g of dextrin and 6 g of ascorbic acid were added to the obtained concentrated liquid, and the mixture was concentrated to dryness under reduced pressure. 600 g of a powdery composition was obtained, the barbaroin content of which was 0.5% by weight.

Examples 6, 7 and Comparative Example 4 Beverages having the compositions shown in Table 3 were produced, filled in bottles, sealed, and then heat-treated at 80 ° C. for 15 minutes for sterilization. Table 3 shows the results of quantifying barvaloin in each beverage after cooling to room temperature.

[0037]

Table 3 Example 6 Example 7 Comparative Example 4 Composition (g / beverage 100 g) Erythritol 15.0 15.0 15.0 Perfume 0.1 0.1 0.1 Aloe extract (Production Example 2) 1.0 -1.0 Powdery composition (Example 5) -0.5-Ascorbic acid 0.1 0.005 (*)- Barvaloin content (wt%) Before heat sterilization 0.003 0.0025 0.003 Heat sterilization After 0.003 0.0025 0.001 (* introduced as part of the composition of Example 5)

[0038]

INDUSTRIAL APPLICABILITY As described above, according to the present invention, it is possible to store barvaloin in an aqueous solution for a long period of time, and it is possible to utilize the pharmacological action of barvaloin in the form of a liquid preparation, a drink or the like that is easy to take. become. It is also possible to extract barvaloin from aloe containing barbaroin in good yield.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁶ Identification number Office reference number FI technical display location // A61K 35/78 A61K 35/78 V

Claims (5)

[Claims] 1. A composition comprising at least one compound selected from the group consisting of L-ascorbic acid and an alkali metal salt thereof, a fatty acid ester, a phosphoric acid ester, or a sugar transfer product, together with barvaloin. 2. L-ascorbic acid and one or more compounds selected from the group consisting of alkali metal salts, fatty acid esters, phosphoric acid esters or sugar transfer products thereof, and barvaloin coexisting in hydrous ethanol. Composition. 3. A beverage containing one or more compounds selected from the group consisting of L-ascorbic acid and its alkali metal salts, fatty acid esters, phosphoric acid esters or sugar transfer products together with barvaloin. 4. An aloe containing barbaroin is extracted with water-containing ethanol, and the obtained extract or its concentrate is L-ascorbic acid and its alkali metal salts, fatty acid esters, phosphoric acid esters, or sugar transfer products. The method for producing the composition according to claim 1, wherein one or more compounds selected from the group consisting of are mixed. 5. Containing barvaloin using hydrous ethanol containing one or more compounds selected from the group consisting of L-ascorbic acid and its alkali metal salts, fatty acid esters, phosphoric acid esters, or sugar transfer products. The method for producing the composition according to claim 1, wherein the aloe obtained is subjected to an extraction treatment, and the obtained extract is concentrated or dried.