JPH09191785A - Plant culture vessel - Google Patents
Plant culture vesselInfo
- Publication number
- JPH09191785A JPH09191785A JP922696A JP922696A JPH09191785A JP H09191785 A JPH09191785 A JP H09191785A JP 922696 A JP922696 A JP 922696A JP 922696 A JP922696 A JP 922696A JP H09191785 A JPH09191785 A JP H09191785A
- Authority
- JP
- Japan
- Prior art keywords
- culture
- lid
- container
- porous membrane
- container body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000012528 membrane Substances 0.000 claims abstract description 32
- 230000035699 permeability Effects 0.000 claims description 24
- 239000000463 material Substances 0.000 claims description 12
- 238000009423 ventilation Methods 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 abstract description 12
- 238000012258 culturing Methods 0.000 abstract description 6
- 244000005700 microbiome Species 0.000 abstract description 5
- 210000000056 organ Anatomy 0.000 abstract description 4
- 239000012298 atmosphere Substances 0.000 abstract description 2
- 241001465754 Metazoa Species 0.000 abstract 1
- 239000007789 gas Substances 0.000 description 11
- 230000000052 comparative effect Effects 0.000 description 9
- 239000011347 resin Substances 0.000 description 9
- 229920005989 resin Polymers 0.000 description 9
- 239000011148 porous material Substances 0.000 description 8
- 238000002834 transmittance Methods 0.000 description 8
- 230000012010 growth Effects 0.000 description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 239000004743 Polypropylene Substances 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 6
- -1 polypropylene Polymers 0.000 description 6
- 229920001155 polypropylene Polymers 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 240000006497 Dianthus caryophyllus Species 0.000 description 4
- 235000009355 Dianthus caryophyllus Nutrition 0.000 description 4
- 244000017020 Ipomoea batatas Species 0.000 description 4
- 235000002678 Ipomoea batatas Nutrition 0.000 description 4
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 3
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 3
- 239000005977 Ethylene Substances 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 3
- 239000004793 Polystyrene Substances 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 239000003429 antifungal agent Substances 0.000 description 3
- 229940121375 antifungal agent Drugs 0.000 description 3
- 239000003963 antioxidant agent Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 239000003086 colorant Substances 0.000 description 3
- 239000000945 filler Substances 0.000 description 3
- 239000012760 heat stabilizer Substances 0.000 description 3
- 229920001684 low density polyethylene Polymers 0.000 description 3
- 239000004702 low-density polyethylene Substances 0.000 description 3
- 239000000314 lubricant Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000003607 modifier Substances 0.000 description 3
- 238000000465 moulding Methods 0.000 description 3
- 229920001778 nylon Polymers 0.000 description 3
- 239000004014 plasticizer Substances 0.000 description 3
- 239000004417 polycarbonate Substances 0.000 description 3
- 229920000515 polycarbonate Polymers 0.000 description 3
- 229920002223 polystyrene Polymers 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- WSSSPWUEQFSQQG-UHFFFAOYSA-N 4-methyl-1-pentene Chemical compound CC(C)CC=C WSSSPWUEQFSQQG-UHFFFAOYSA-N 0.000 description 2
- 239000004952 Polyamide Substances 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000001746 injection moulding Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 2
- 229920002647 polyamide Polymers 0.000 description 2
- 229920005668 polycarbonate resin Polymers 0.000 description 2
- 239000004431 polycarbonate resin Substances 0.000 description 2
- 229920000728 polyester Polymers 0.000 description 2
- 239000004926 polymethyl methacrylate Substances 0.000 description 2
- 239000004800 polyvinyl chloride Substances 0.000 description 2
- 229920000915 polyvinyl chloride Polymers 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241001312221 Anthurium Species 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000009849 Cucumis sativus Nutrition 0.000 description 1
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 241000735332 Gerbera Species 0.000 description 1
- 241001316290 Gypsophila Species 0.000 description 1
- 241000234435 Lilium Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000000956 alloy Substances 0.000 description 1
- 229910045601 alloy Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000002981 blocking agent Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 229920000092 linear low density polyethylene Polymers 0.000 description 1
- 239000004707 linear low-density polyethylene Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 235000021012 strawberries Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
(57)【要約】
【目的】 動植物あるいは微生物の細胞、組織、器官あ
るいは個体全体を長期間培養するための培養容器であ
り、その内部雰囲気中のO2 、CO2 濃度を最適化し、
育成するための培養容器を提供する。
【構成】 光透過性を有する容器本体とこの容器本体の
上端開口部を閉止する蓋からなり、開口部が広く操作性
に優れた、高い密閉性を保つことを特徴とする容器であ
って、さらに、蓋に通気口が設けられており、この通気
口にガス透過性を有する多孔膜を用い、しかも多孔膜を
簡易に取り替え出来る構造としたことを特徴とする培養
容器。
(57) [Summary] [Purpose] A culture vessel for culturing cells, tissues, organs or whole individuals of plants and animals or microorganisms for a long period of time, optimizing the O 2 and CO 2 concentrations in the internal atmosphere,
Provide a culture container for growing. A container characterized by comprising a light-transmissive container body and a lid for closing an upper end opening of the container body, having a wide opening, excellent operability, and maintaining high hermeticity, Further, the culture container is characterized in that the lid is provided with a vent, and the vent is made of a gas permeable porous membrane, and the porous membrane can be easily replaced.
Description
【0001】[0001]
【発明の属する技術分野】本発明は植物の細胞、組織、
器官あるいは個体全体(以下、内容物と言うことがあ
る)を簡易に効率よく密封し、長期間培養するための植
物培養容器(以下、培養容器と言うことがある)に関す
るものである。更に詳しくは、容器本体は光透過性を有
し、この容器本体の上端開口部を閉止する蓋に設けられ
た通気口を、高通気性を有する多孔膜で閉止することに
より培養容器内のガス、水、光環境を最適に保つ上記の
内容物に最適の生育環境を提供する植物培養容器に関す
るものである。TECHNICAL FIELD The present invention relates to plant cells, tissues,
The present invention relates to a plant culture vessel (hereinafter sometimes referred to as culture vessel) for easily and efficiently sealing an organ or the whole body (hereinafter sometimes referred to as contents) and culturing for a long period of time. More specifically, the container body has a light-transmitting property, and the gas in the culture container is closed by closing the vent hole provided in the lid that closes the upper end opening of the container body with a porous membrane having high air permeability. The present invention relates to a plant culture container that provides an optimal growth environment for the above contents, which keeps the water and light environments optimal.
【0002】[0002]
【従来の技術】培養容器は、植物の細胞、組織、器官等
を培養するための環境を与えるために種々の性質、たと
えば、光透過性、通気性、保湿性、耐久性、耐薬品性な
どを満足することが要求される。これらの性質を満足す
るために、従来よりガラス製もしくは各種樹脂製の培養
容器が用いられてきた。2. Description of the Related Art Culture vessels have various properties such as light transmission, breathability, moisture retention, durability and chemical resistance in order to provide an environment for culturing plant cells, tissues and organs. Is required to be satisfied. In order to satisfy these properties, culture vessels made of glass or various resins have been conventionally used.
【0003】しかし、これらの培養容器は通気性が悪い
ため、培養物の呼吸や光合成に必要な酸素や炭酸ガスを
供給するために、ねじ口栓を緩めて使ったり、綿栓、ペ
ーパーストッパー、アルミホイル等を使ってガス交換を
行っているが、十分な通気性を確保できない場合が多
い。また、通気性を確保するためには上述の開口部の栓
類を常に緩めておく必要性から培地の散逸が大きくな
り、ために培地の濃度が上がるなど培養物の生育を阻害
する、常に雑菌の侵入の危険にさらされる等、の問題が
ある。さらに、これらの培養容器は開口部が狭く、培養
物の操作が非常に困難であり、培養物を出し入れする操
作には多くの経験と、時間を要し、時として培養物に損
傷を与え収率が低下する等、多くの問題があるのが実状
である。However, since these culture vessels have poor air permeability, they can be used by loosening the screw caps, cotton plugs, paper stoppers, etc. in order to supply oxygen and carbon dioxide required for respiration and photosynthesis of the cultures. Although gas is exchanged using aluminum foil, etc., it is often the case that sufficient ventilation cannot be ensured. Further, in order to ensure air permeability, it is necessary to always loosen the plugs of the above-mentioned opening, so that the dissipation of the medium becomes large, and therefore the concentration of the medium is increased, which inhibits the growth of the culture, and is always a bacteria. There are problems such as being exposed to the risk of intrusion. In addition, these culture vessels have narrow openings, making it very difficult to manipulate the culture, and the operation of loading and unloading the culture requires a lot of experience and time, and sometimes the culture is damaged and damaged. The reality is that there are many problems, such as a decrease in the rate.
【0004】そこで、光透過性、耐久性などの諸性質を
保持しながら、雑菌の侵入を伴うことなく培養物の育成
に十分な通気性を有し、培地の散逸が少なく且つ、操作
性のよい培養容器が求められている。上記の目的を達す
るために、ガス透過性の高い含フッ素溶融樹脂フィルム
を袋状に加工成形した培養容器が提案されている(特開
昭63−198972号公報参照)。しかし、一部の培
養対象または培養条件については更に高いガス透過性が
必要であること、フッ素樹脂は溶融温度が約300℃と
高いために、フィルム加工時の熱接着操作が容易でない
ことなどが明らかとなった。また含フッ素樹脂は比較的
高価であり操作性に劣る等、実用的ではない。Therefore, while maintaining various properties such as light transmittance and durability, it has sufficient air permeability for growing a culture without invasion of various bacteria, has a small loss of medium, and is easy to operate. There is a need for good culture vessels. In order to achieve the above object, a culture container in which a fluorine-containing molten resin film having high gas permeability is processed into a bag shape has been proposed (see JP-A-63-198972). However, for some culture targets or culture conditions, higher gas permeability is required, and since the melting temperature of fluororesin is as high as about 300 ° C, the heat bonding operation during film processing is not easy. It became clear. Further, the fluororesin is not practical because it is relatively expensive and inferior in operability.
【0005】特開平3ー277268号公報には低密度
ポリエチレンと線状低密度ポリエチレンとのポリマーア
ロイからなるフィルムをもちいた細胞培養バッグが示さ
れているが、低密度ポリエチレンもガス透過性は充分と
は言えず、バック状の形態では操作性にも劣る等、の問
題があった。さらに近年、一部の培養容器に多孔膜が用
いられはじめているが、これら多孔膜の素材は一般的に
光線透過率の高い物質が使用されるものの、多孔化する
ことにより光線の乱反射が起き、ために光合成が重要視
される培養容器にこのような不透明な素材を使用するこ
とには大きな抵抗があった。Japanese Unexamined Patent Publication (Kokai) No. 3-277268 discloses a cell culture bag using a film made of a polymer alloy of low density polyethylene and linear low density polyethylene, but low density polyethylene also has sufficient gas permeability. However, there is a problem that the bag-like form is inferior in operability. Furthermore, in recent years, porous membranes have begun to be used in some culture vessels, but the material of these porous membranes is generally a substance having a high light transmittance, but diffused reflection of light rays occurs due to porosity, Therefore, there was great resistance to use such an opaque material in a culture container where photosynthesis is important.
【0006】また、培養容器の多くは使用前に滅菌処理
を施す必要があるため、この処理温度に耐えるうる多孔
膜素材は制限され、しかも、高通気性を要求される培養
容器には必然的に広い面積の多孔膜を用いるほかなく、
従って光線を遮蔽する多孔膜の使用には自ずと限界があ
った。加えて多孔膜を使用する方法として、多孔膜に粘
着剤を塗布し、培養容器に貼り合わせるなどの手法が取
られてきたため、多孔膜の取り替えが容易に行えない等
の問題があり、実用的ではない。Further, since many culture vessels need to be sterilized before use, the porous membrane material that can withstand this treatment temperature is limited, and it is inevitable for culture vessels that require high air permeability. There is no choice but to use a large area porous membrane
Therefore, there has been a limit to the use of a porous film that shields light rays. In addition, as a method of using the porous membrane, since a method of applying an adhesive to the porous membrane and attaching it to the culture container has been taken, there is a problem that the replacement of the porous membrane cannot be easily performed, and thus it is practical. is not.
【0007】[0007]
【発明が解決しようとする課題】そこで、本発明におい
ては、前述の多種多様な培養物の最適環境を作り出し、
しかも、密閉性が高く操作性のよい培養容器を提供する
ことを課題とする。Therefore, in the present invention, the optimum environment for the various cultures described above is created,
Moreover, it is an object of the present invention to provide a culture container that is highly sealed and has good operability.
【0008】[0008]
【課題を解決するための手段】本発明の目的は上記課題
を解決するために、光透過性、耐久性などの諸性質を保
持しながら雑菌侵入の恐れのない高通気性を有する多孔
膜を用いた密閉性が高く操作性の良い培養容器を提供す
ることにある。In order to solve the above-mentioned problems, an object of the present invention is to provide a porous membrane having various properties such as light transmittance and durability while having high air permeability without fear of invading bacteria. An object of the present invention is to provide a culture container having a high airtightness and good operability.
【0009】本発明の要旨は、光透過性を有する容器本
体の上端開口部を閉止する蓋に設けた通気口を、通気性
を有する多孔膜で閉止されてなることを特徴とする培養
容器に存する。[0009] The gist of the present invention is a culture container characterized in that the vent hole provided in the lid for closing the upper end opening of the container body having a light-transmitting property is closed with a porous film having a gas permeability. Exist.
【0010】[0010]
【発明の実施の形態】以下、本発明を詳細に説明する。
本発明の培養容器は容器本体と容器本体の開口部を閉止
する蓋、および蓋の通気口を閉止する多孔膜、多孔膜を
保持するキャップからなる。容器本体及び蓋は厚み1m
mで可視領域の波長400mμ以上の範囲での光線透過
率が80%以上の光透過性を持つ樹脂で、例えば、ポリ
スチレン、ポリエステル、ポリアミド、ポリカーボネー
ト、ポリ4−メチルペンテン−1、ポリメチルメタアク
リレート、ポリ塩化ビニルなどから選ばれるが、好まし
くは耐久性、耐熱性等に優れるポリカーボネート樹脂が
使用される。 また、これらの樹脂には通常成形時に添
加される可塑剤、熱安定剤、酸化防止剤、充填剤、滑
剤、着色剤、改質剤などは、必要に応じて使用しても差
し支えない。さらに、植物や微生物の培養上重要な抗菌
剤、抗カビ剤、防曇剤、エチレン除去剤などの添加も可
能である。BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below.
The culture container of the present invention comprises a container body, a lid that closes the opening of the container body, a porous membrane that closes the vent hole of the lid, and a cap that holds the porous membrane. 1m thick container body and lid
A resin having a light transmittance of 80% or more in a visible wavelength range of 400 mμ or more, such as polystyrene, polyester, polyamide, polycarbonate, poly-4-methylpentene-1, polymethylmethacrylate. , Polyvinyl chloride, etc., but a polycarbonate resin having excellent durability and heat resistance is preferably used. Further, plasticizers, heat stabilizers, antioxidants, fillers, lubricants, colorants, modifiers and the like, which are usually added at the time of molding, may be used for these resins, if necessary. Further, it is possible to add antibacterial agents, antifungal agents, antifogging agents, ethylene removing agents, etc., which are important for culturing plants and microorganisms.
【0011】多孔膜を保持するキャップは蓋の通気口と
嵌合して密閉性を保つ事が可能であれば容器本体と容器
本体の開口部を閉止する蓋と同種の樹脂でも良く、更に
は汎用の樹脂でも良い。汎用の樹脂のなかでも低密度ポ
リエチレン、ポリプロピレン等が好んで用いられるが、
柔軟性を持たせ密閉性を向上させるためにこれらに可塑
剤等を添加しても良い。The cap for holding the porous membrane may be made of the same resin as the container body and the lid for closing the opening of the container body, as long as it can be fitted with the vent hole of the lid to maintain the airtightness. General-purpose resin may be used. Among general-purpose resins, low density polyethylene, polypropylene, etc. are preferred, but
A plasticizer or the like may be added to these in order to impart flexibility and improve the hermeticity.
【0012】さらに容器本体と同様にして、これらの樹
脂には通常成形時に添加される熱安定剤、酸化防止剤、
充填剤、滑剤、アンチブロッキング剤、紫外線吸収剤、
着色剤、改質剤などは、必要に応じて使用しても差し支
えない。さらに、植物や微生物の培養上重要な抗菌剤、
抗カビ剤、防曇剤、エチレン除去剤などの添加も可能で
ある。Further, in the same manner as the container body, a heat stabilizer, an antioxidant, which is usually added during molding, is added to these resins.
Filler, lubricant, anti-blocking agent, UV absorber,
Colorants, modifiers and the like may be used as necessary. In addition, an antibacterial agent, which is important for culturing plants and microorganisms,
It is also possible to add antifungal agents, antifogging agents, ethylene removing agents and the like.
【0013】蓋の通気口を閉止する多孔膜は、通気度が
1〜50sec/100cc、好ましくは1〜10se
c/100ccである。1sec/100cc未満であ
ると容器内の保湿性が保たれないため好ましくない。一
方、50sec/100ccを越えると通気性が悪くな
るので充分な生育が得られない。最大孔径が15μm以
下であり、かつ平均孔径が0.1〜10μmであること
が好ましく、更には0.1〜5μmが好適である。平均
孔径が10μmを越えると、雑菌等の混入が懸念され好
ましくない。また、厚みは取り扱い上50〜500μm
の範囲の物が好ましい。The porous membrane for closing the vent hole of the lid has an air permeability of 1 to 50 sec / 100 cc, preferably 1 to 10 se.
It is c / 100cc. If it is less than 1 sec / 100 cc, the moisture retaining property in the container cannot be maintained, which is not preferable. On the other hand, if it exceeds 50 sec / 100 cc, the air permeability is deteriorated, and sufficient growth cannot be obtained. The maximum pore size is preferably 15 μm or less, and the average pore size is preferably 0.1 to 10 μm, and more preferably 0.1 to 5 μm. If the average pore size exceeds 10 μm, contamination of various bacteria may occur, which is not preferable. Also, the thickness is 50 to 500 μm in terms of handling.
Those in the range of are preferred.
【0014】植物培養苗の生育には容器内の湿度が通常
70〜90%程度が好ましく、更には75〜85%がよ
り好ましい。この多孔膜の素材としては滅菌処理条件に
耐えうる物であれば良く、例えば、ポリスチレン、ポリ
エステル、ポリアミド、ポリカーボネート、ポリ4−メ
チルペンテン−1、ポリメチルメタアクリレート、ポリ
プロピレン、ポリ塩化ビニル、ナイロン、セルロース、
フッ素樹脂などが挙げられる。更にはこれらの複合化、
もしくは耐久性、耐熱性等を増すためにこれらを他の基
材と複合化したものも可能である。また、これらには通
常成形時に添加される可塑剤、熱安定剤、酸化防止剤、
充填剤、滑剤、着色剤、改質剤などは、必要に応じて使
用しても差し支えない。さらに、植物や微生物の培養上
重要な抗菌剤、抗カビ剤、防曇剤、エチレン除去剤など
の添加も可能である。本発明の培養容器はこの多孔膜を
培養期間中は隙間なく保持し、多孔膜を取り替える際に
は簡易に交換可能な構造としたキャップを用いた培養容
器である。For the growth of plant culture seedlings, the humidity in the container is preferably about 70 to 90%, more preferably 75 to 85%. The material of the porous film may be any one that can withstand sterilization conditions, and examples thereof include polystyrene, polyester, polyamide, polycarbonate, poly-4-methylpentene-1, polymethylmethacrylate, polypropylene, polyvinyl chloride, nylon, cellulose,
Examples thereof include fluororesins. Furthermore, compounding these,
Alternatively, it is possible to combine these with other base materials in order to increase durability, heat resistance and the like. In addition, plasticizers, heat stabilizers, antioxidants, which are usually added during molding,
Fillers, lubricants, colorants, modifiers and the like may be used as necessary. Further, it is possible to add antibacterial agents, antifungal agents, antifogging agents, ethylene removing agents, etc., which are important for culturing plants and microorganisms. The culture container of the present invention is a culture container using a cap which holds this porous membrane without any gaps during the culture period and can be easily exchanged when replacing the porous membrane.
【0015】以下、本発明の培養容器の形状およびその
使用方法の一例について添付図を用いて更に詳細に説明
する。図1は本発明の培養容器使用例である。この図に
おいて10は容器本体、20は蓋である。容器本体は、
光透過性を有する材質、例えばポリスチレン、ポリカー
ボネートなどからなり、開口部より底部の寸法が小さ
く、傾斜が付いた有底四角柱状である。蓋20には通気
口に多孔膜40がキャップ30でセットされている。5
0は培地、60は培養物である。Hereinafter, an example of the shape of the culture container of the present invention and a method of using the culture container will be described in more detail with reference to the accompanying drawings. FIG. 1 shows an example of using the culture container of the present invention. In this figure, 10 is a container body and 20 is a lid. The container body is
It is made of a light-transmissive material, such as polystyrene or polycarbonate, and has a bottom portion having a smaller dimension than the opening portion, and is an inclined bottomed rectangular column. A porous film 40 is set in the lid 20 with a cap 30 at the vent. 5
0 is a medium and 60 is a culture.
【0016】図2は容器本体10の詳細図である。この
容器本体10の上端開口部11は角に丸みをもたせた四
角形に形成され、この開口部には鍔部12が周設され、
これに連続して下方に向かって側面傾斜部13が形成さ
れ、それらの下部に容器本体の底部を塞ぐ底板14が一
体に設けられている。FIG. 2 is a detailed view of the container body 10. An upper end opening 11 of the container body 10 is formed in a quadrangle with rounded corners, and a flange 12 is provided around the opening.
Continuing to this, side surface inclined portions 13 are formed downward, and a bottom plate 14 that closes the bottom portion of the container body is integrally provided below them.
【0017】図3は容器本体の開口部を閉止する蓋20
の詳細図である。この蓋20は角に丸みをもたせた四角
形に形成され、周設された鍔部21は、容器本体の上端
開口部の鍔部12に嵌合される。さらに22は蓋に設け
た通気口、23は多孔膜と密着する端面、24は多孔膜
40を保持するキャップ30に嵌合される鍔部である。FIG. 3 shows a lid 20 for closing the opening of the container body.
FIG. The lid 20 is formed in a quadrangular shape with rounded corners, and the flange portion 21 provided around the lid 20 is fitted into the flange portion 12 at the upper end opening of the container body. Further, 22 is a vent hole provided in the lid, 23 is an end face that comes into close contact with the porous membrane, and 24 is a flange portion fitted into the cap 30 holding the porous membrane 40.
【0018】図4は蓋に設けた通気口を閉止する多孔膜
を隙間なく保持するキャップ30の詳細図である。この
キャップの上端には通気口31が設けられており、32
は多孔膜40を保護する桟、33は蓋20の通気口に設
けられた鍔部24に嵌合される鍔部である。FIG. 4 is a detailed view of the cap 30 which holds the porous film for closing the vent hole provided in the lid without any gap. A vent 31 is provided at the upper end of this cap,
Is a crosspiece that protects the porous membrane 40, and 33 is a flange that is fitted to the flange 24 provided at the vent of the lid 20.
【0019】図5は蓋20の通気口22に多孔膜40を
保持したキャップ30の拡大断面図である。FIG. 5 is an enlarged sectional view of the cap 30 in which the porous film 40 is held in the vent hole 22 of the lid 20.
【0020】本発明の培養容器には一般の培地が使用さ
れる。例えば、ホワイト(White)培地、ムラシゲ
とスクーグ(Murashige & Skoog)培
地、ヘラー(Heller)培地、ヴァシンとヴェント
(Vacin & Went)培地、および、これらの
修正培地などが挙げられる。培地には、必要に応じ、植
物ホルモン、抗生物質などを加えても良い。A general medium is used in the culture container of the present invention. Examples thereof include White medium, Murashige & Skoog medium, Heller medium, Vacin & Went medium, and modified mediums thereof. If necessary, a plant hormone, an antibiotic, etc. may be added to the medium.
【0021】本発明の培養容器は、通気性があり、光透
過性にも優れているため、高炭酸ガス雰囲気の培養装置
内において、培養中の植物または微生物などの細胞、組
織、器官もしくは個体全体などに対して十分にガス交換
することができる。培養装置としては、炭酸ガス供給管
や照明装置などを備えた通常の培養装置が使用される。
更には、一般の培養室内で使用されることは勿論であ
る。Since the culture container of the present invention has air permeability and excellent light transmittance, cells, tissues, organs or individuals such as plants or microorganisms in culture are cultured in a culture device in a high carbon dioxide atmosphere. It is possible to sufficiently exchange the gas for the whole. As the culture device, a normal culture device equipped with a carbon dioxide gas supply pipe, a lighting device and the like is used.
Further, it is of course used in a general culture room.
【0022】本発明の培養容器が適用できる対象植物と
しては特に制限されないが、好ましくは、ラン、ユリ、
カーネーション、カスミソウ、ガーベラ、シンビジュウ
ム、アンスリュウム、スパティフィラム等の花弁類、イ
チゴ、サツマイモ、トマト、キュウリ等の果菜類などが
挙げられる。The target plant to which the culture container of the present invention can be applied is not particularly limited, but preferably orchid, lily,
Examples include petals such as carnations, gypsophila, gerbera, symbidium, anthurium, and spatiphyllum, and fruit vegetables such as strawberries, sweet potatoes, tomatoes, and cucumbers.
(実施例1)次に、本発明の実施例についてに更に詳し
く説明するが、本発明は実施例に限定されるものではな
い。なお、光線透過率の測定は紫外/可視分光光度計
(積分球使用)(島津製作所製UV−3100S型)、
多孔膜の通気度の測定はB型ガーレー式デンソーメータ
ー(東洋精機製作所製、JIS規格P8117に準拠)
で行い、また、葉色の測定は簡易葉緑素計(ミノルタ社
製SPAD−502型)を用いて測定した。Example 1 Next, examples of the present invention will be described in more detail, but the present invention is not limited to the examples. In addition, the light transmittance is measured by an ultraviolet / visible spectrophotometer (using an integrating sphere) (UV-3100S type manufactured by Shimadzu Corporation),
The air permeability of the porous membrane is measured by B-type Gurley type densometer (manufactured by Toyo Seiki Seisakusho, conforming to JIS standard P8117).
The measurement of leaf color was carried out using a simple chlorophyll meter (SPAD-502 type manufactured by Minolta Co., Ltd.).
【0023】容器本体はポリカーボネート樹脂を用い、
底部は80mm×80mm、高さ130mm、開口部は
100mm×100mm、厚さ1.5mmのものを射出
成形法で成形した。この容器本体の光線透過率は、可視
領域の波長400mμ以上の範囲において85%以上で
あった。蓋は10φ(直径10mm)の通気口を有し、
容器本体開口部の鍔部に嵌合するものを容器本体と同様
にして射出成形法で成形した。この蓋の光線透過率は、
可視領域の波長400mμ以上の範囲において85%以
上であった。Polycarbonate resin is used for the container body,
The bottom part was 80 mm × 80 mm, the height was 130 mm, the opening part was 100 mm × 100 mm, and the thickness was 1.5 mm. The light transmittance of this container body was 85% or more in the visible wavelength range of 400 mμ or more. The lid has a ventilation hole of 10φ (diameter 10 mm),
What was fitted to the flange of the opening of the container body was molded by the injection molding method in the same manner as the container body. The light transmittance of this lid is
It was 85% or more in the visible wavelength range of 400 mμ or more.
【0024】多孔膜を保持するキャップは、ポリプロピ
レン樹脂を用い蓋の通気口に多孔膜を隙間なく保持で
き、且つ、キャップの上部には通気口を設け、蓋の通気
口の鍔部に嵌合するものを射出成形法で成形した。つい
で、この蓋の通気口を閉止する多孔膜E01008E
(日本ポール社製多孔膜、厚み:200μm、公称平均
孔径:0.3μm、通気度:2sec/100cc、素
材・構成:ガラス繊維メッシュ/セルロースフィルタ
ー)を15φの円形に打ち抜きキャップの内側からセッ
トし、キャップを蓋の通気口の鍔部に嵌合させ培養容器
を得た。The cap for holding the porous film is made of polypropylene resin so that the porous film can be held in the vent hole of the lid without a gap, and the vent hole is provided on the upper part of the cap, and the cap is fitted to the collar portion of the vent port of the lid. Was formed by an injection molding method. Then, a porous membrane E01008E for closing the vent of this lid is closed.
(Nihon Pole's porous membrane, thickness: 200 μm, nominal average pore diameter: 0.3 μm, air permeability: 2 sec / 100 cc, material / structure: glass fiber mesh / cellulose filter) are set in a circle of 15φ from the inside of the punch cap. The cap was fitted to the collar of the vent of the lid to obtain a culture container.
【0025】この容器本体10に有糖寒天培地(Mur
ashige & Skoog)を130cc注入し、
容器本体10の鍔部12に蓋20の鍔部21を嵌合させ
密閉した。この容器をオートクレーブに入れ120℃で
−20分間の滅菌処理を施し、クリーンベンチを使用
し、無菌操作によりカーネーション苗の1節を25本置
床した。同様にしてサツマイモ苗の1節を25本置床し
たものをそれぞれ、培養室内で30日間培養した。この
結果(平均値)について、カーネーション苗は表−1
に、サツマイモ苗は表−2に示す。In this container body 10, a sugar-containing agar medium (Mur
inject 130 cc of ashige & Skoog,
The flange portion 21 of the lid 20 was fitted into the flange portion 12 of the container body 10 and hermetically sealed. This container was placed in an autoclave, sterilized at 120 ° C. for -20 minutes, and 25 sections of carnation seedlings were placed by aseptic operation using a clean bench. In the same manner, 25 one-section sections of sweet potato seedlings were placed, and each was cultured in a culture chamber for 30 days. Table 1 shows the results (average value) of carnation seedlings.
Table 2 shows the sweet potato seedlings.
【0026】(実施例2)蓋の通気口を閉止する多孔膜
をHydrolon(日本ポール社製多孔膜、厚み:1
60μm、平均孔径:1.2μm、通気度:7sec/
100cc、素材:ナイロン)にした以外は実施例1と
同様にして培養室内で30日間培養した。この結果を表
に示す。(Example 2) The porous membrane for closing the vent of the lid was made of Hydrolon (manufactured by Nippon Pall Co., thickness: 1
60 μm, average pore size: 1.2 μm, air permeability: 7 sec /
Culture was carried out for 30 days in the culture chamber in the same manner as in Example 1 except that 100 cc, material: nylon) was used. The results are shown in the table.
【0027】(実施例3)蓋の通気口を閉止する多孔膜
をBiodyneA(日本ポール社製多孔膜、厚み:1
50μm、平均孔径:0.5μm、通気度:30sec
/100cc、素材:ナイロン)にした以外は実施例1
と同様にして培養室内で30日間培養した。この結果を
表に示す。(Example 3) Biodyne A (a porous film manufactured by Nippon Pall Co., thickness: 1) was used as a porous film for closing the vent of the lid.
50 μm, average pore size: 0.5 μm, air permeability: 30 sec
/ 100cc, material: nylon) Example 1 except that
The cells were cultured in the culture chamber for 30 days in the same manner as in. The results are shown in the table.
【0028】(実施例4)蓋の通気口を閉止する多孔膜
をHDCIIJ012(日本ポール社製多孔膜、厚み:1
50μm、平均孔径:1.2μm、通気度:44sec
/100cc、素材:ポリプロピレン)にした以外は実
施例1と同様にして培養室内で30日間培養した。この
結果を表に示す。(Example 4) A porous film for closing the vent of the lid was HDCIIJ012 (a porous film manufactured by Nippon Pall Co., thickness: 1).
50 μm, average pore size: 1.2 μm, air permeability: 44 sec
/ 100 cc, material: polypropylene), and the culture was carried out in the culture chamber for 30 days in the same manner as in Example 1. The results are shown in the table.
【0029】(比較例1)蓋の通気口を閉止する多孔膜
を通気性のない無孔のポリプロピレンフィルム(50
μ)とした以外は実施例1と同様にして培養室内で30
日間培養した。この結果を表に示す。(Comparative Example 1) A non-porous polypropylene film (50) having no air permeability was used as a porous film for closing the vent hole of the lid.
μ) in the same manner as in Example 1 except that
Cultured for days. The results are shown in the table.
【0030】(比較例2)蓋の通気口を閉止する多孔膜
をHDCIIJ006(日本ポール社製多孔膜、厚み:2
00μm、平均孔径:0.6μm、通気度:70sec
/100cc、素材:ポリプロピレン)にした以外は実
施例1と同様にして培養室内で30日間培養した。この
結果を表に示す。(Comparative Example 2) A porous film for closing the vent of the lid was HDCIIJ006 (a porous film manufactured by Nippon Pall Co., thickness: 2).
00 μm, average pore size: 0.6 μm, air permeability: 70 sec
/ 100 cc, material: polypropylene), and the culture was carried out in the culture chamber for 30 days in the same manner as in Example 1. The results are shown in the table.
【0031】(比較例3)蓋の通気口を閉止する多孔膜
の替わりに綿を使用した以外は実施例1と同様にして培
養室内で30日間培養した。この結果を表に示す。尚、
この綿栓の通気度は0.7sec/100ccであっ
た。(Comparative Example 3) The culture was carried out for 30 days in the culture chamber in the same manner as in Example 1 except that cotton was used instead of the porous membrane for closing the vent of the lid. The results are shown in the table. still,
The air permeability of this cotton plug was 0.7 sec / 100 cc.
【0032】[0032]
【表1】 表−1 カーネーション苗 生重 草丈 節数 根量* 葉色 水浸状 総合評価 g/本 cm 0〜++ SPAD値 % 苗質 実施例1 0.30 7.0 6.0 + 45 0 非常に良好 2 0.25 7.0 6.0 + 44 0 良好 3 0.20 6.4 5.5 + 39 0 普通 4 0.20 6.4 5.5 + 36 0 普通 比較例1 0.15 5.8 5.0 + 33 10 悪(劣る) 比較例2 0.16 6.0 5.0 + 33 8 悪(劣る) 比較例3 全て枯死 (液体培地散逸、寒天固化) * 根量:0なし、+普通、++多い [Table 1] Table-1 Carnation seedling fresh weight Plant height Node number Root volume * Leaf color Water immersion Overall evaluation g / piece cm 0 to + + SPAD value% Seedling quality Example 1 0.30 7.0 6.0 + 45 0 Very good 2 0.25 7.0 6.0 + 44 0 Good 3 0.20 6.4 5.5 + 39 0 Normal 4 0.20 6.4 5.5 + 36 0 Normal Comparative Example 1 0.15 5.8 5.0 + 33 10 Bad (Inferior) Comparative Example 2 0.16 6.0 5.0 + 33 8 Bad (Inferior) Comparative Example 3 All dead (dissipation of liquid medium, solidification of agar) * Root mass: 0 none, + normal, + large
【0033】[0033]
【表2】 表−2 サツマイモ苗 生重 草丈 葉数 最大葉長 葉色 総合評価 g/本 cm 枚 cm SPAD値 苗質 実施例1 0.9 3.0 9 3.0 45 非常に良好 2 0.8 3.0 8 2.9 44 良好 3 0.7 2.8 8 2.7 38 普通 4 0.7 2.7 8 2.7 37 普通 比較例1 0.6 2.5 7 2.5 33 悪(劣る) 比較例2 0.6 2.6 7 2.6 33 悪(劣る) 比較例3 全て枯死 (液体培地散逸、寒天固化) [Table 2] Table-2 Sweet potato seedling fresh weight Plant height Leaf number Maximum leaf length Leaf color Comprehensive evaluation g / piece cm Sheet cm SPAD value Seedling quality Example 1 0.9 3.0 9 3.0 45 Very good 2 0.8 3.0 8 2.9 44 Good 3 0.7 2.8 8 2.7 38 Normal 4 0.7 2.7 8 2.7 37 Normal Comparative Example 1 0.6 2.5 7 2.5 33 Bad (Inferior) Comparative Example 2 0.6 2.6 7 2.6 33 Bad (Inferior) Comparative Example 3 All withered (dispersion of liquid medium, solidification of agar)
【0034】[0034]
【発明の効果】実施例の多孔膜を用いた培養容器で培養
した苗の生育状態は良好であり、特にガス透過性の高い
多孔膜を用いた培養容器で培養した苗の生育状態は、非
常に良好であった。INDUSTRIAL APPLICABILITY The seedlings cultivated in the culture vessel using the porous membrane of the example have a good growth state, and particularly, the seedlings cultivated in the culture vessel using the porous membrane having a high gas permeability have a very high growth state. Was very good.
【0035】本発明の培養容器は表に示す通り培養した
苗の生育状態は極めて良好であり、しかも、従来の培養
容器と比較し開口部が広く、かつ、密閉操作も容易であ
り、内容物の植え付け、取り出しの際も特別な経験も不
要で短時間に簡易に行え、雑菌に汚染されることもな
い。さらに、光透過性容器とガス透過性の高い多孔膜を
用いることにより、培養容器内のガス、水、光環境を最
適に保ち、内容物に最適の生育環境を作り出すことを可
能とし、また多孔膜の取り替えを簡易に行える構造とし
たことで、作業性も向上した。容器の製法は従来の汎用
製法で行えるので、安価で実用的な培養容器を提供する
ことができる。As shown in the table, the culture container of the present invention has a very good growth condition of the cultured seedlings, has a wider opening than the conventional culture container, and can be easily sealed. No special experience is required for planting or removing, and it can be done easily in a short time without being contaminated by various bacteria. Furthermore, by using a light-permeable container and a porous membrane with high gas permeability, it is possible to keep the gas, water, and light environment inside the culture container optimal, and create an optimal growth environment for the contents. Workability has also been improved by adopting a structure that allows easy replacement of the membrane. Since the container can be manufactured by the conventional general-purpose manufacturing method, an inexpensive and practical culture container can be provided.
【図1】本発明の組立縦断面図である。FIG. 1 is an assembled vertical sectional view of the present invention.
【図2】本発明の容器本体の斜視図である。FIG. 2 is a perspective view of the container body of the present invention.
【図3】本発明の蓋の斜視図である。FIG. 3 is a perspective view of the lid of the present invention.
【図4】本発明のキャップの斜視図である。FIG. 4 is a perspective view of the cap of the present invention.
【図5】本発明の蓋開口部の組立拡大断面図である。FIG. 5 is an enlarged sectional view of the assembly of the lid opening portion of the present invention.
10 容器本体 11 容器本体上端開口部 12 容器本体鍔部 13 容器本体側面テーパー部 14 容器本体底板 20 蓋 21 蓋鍔部 22 通気口 23 通気口端面 24 通気口鍔部 30 キャップ 31 通気口 32 桟 33 鍔部 40 多孔膜 50 培地 60 培養物 10 Container Main Body 11 Container Main Body Top Opening 12 Container Main Body Collar 13 Container Main Body Side Side Tapered 14 Container Main Body Bottom Plate 20 Lid 21 Lid Collar 22 Vent 23 End Vent 24 End Vent 30 Cap 31 Vent 32 Spiral 33 Collar 40 Porous membrane 50 Medium 60 Culture
Claims (2)
形成された容器本体と蓋からなる培養容器であって、容
器本体は、培養物を収容するための底部と側壁とを有
し、その側壁の上端開口部に蓋と嵌合する鍔部を形成
し、蓋は、容器本体と嵌合する鍔部を周設し、その内側
に通気口を有し、さらには、この通気口が通気度1〜5
0sec/ccの通気性を有する多孔膜で閉止されてな
ることを特徴とする植物培養容器。1. A culture container comprising a container body formed of a light-transmissive plastic material and a lid, the container body having a bottom and a side wall for accommodating a culture. A flange portion that fits with the lid is formed in the upper end opening, and the lid has a collar portion that fits with the container body, and has a vent hole inside thereof, and further, this vent hole has a ventilation degree of 1 ~ 5
A plant culture vessel, which is closed with a porous membrane having an air permeability of 0 sec / cc.
構造を有することを特徴とする請求項1に記載の植物培
養容器。2. The plant culture container according to claim 1, which has a cap structure in which a porous membrane is held at a vent of the lid.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP922696A JPH09191785A (en) | 1996-01-23 | 1996-01-23 | Plant culture vessel |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP922696A JPH09191785A (en) | 1996-01-23 | 1996-01-23 | Plant culture vessel |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH09191785A true JPH09191785A (en) | 1997-07-29 |
Family
ID=11714512
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP922696A Pending JPH09191785A (en) | 1996-01-23 | 1996-01-23 | Plant culture vessel |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH09191785A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000005942A1 (en) * | 1998-07-28 | 2000-02-10 | Institute Of Genetics, Chinese Academy Of Sciences | Culture container and process for producing potato microtubers by using the same |
JPWO2003042352A1 (en) * | 2001-11-16 | 2005-03-10 | ハイトカルチャ株式会社 | Biological culture apparatus and biological culture method |
-
1996
- 1996-01-23 JP JP922696A patent/JPH09191785A/en active Pending
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000005942A1 (en) * | 1998-07-28 | 2000-02-10 | Institute Of Genetics, Chinese Academy Of Sciences | Culture container and process for producing potato microtubers by using the same |
JPWO2003042352A1 (en) * | 2001-11-16 | 2005-03-10 | ハイトカルチャ株式会社 | Biological culture apparatus and biological culture method |
US7972840B2 (en) | 2001-11-16 | 2011-07-05 | Phytoculture Control Co., Ltd. | Apparatus for culturing organism and method of culturing organism |
JP4744082B2 (en) * | 2001-11-16 | 2011-08-10 | ハイトカルチャ株式会社 | Biological culture apparatus and biological culture method |
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