JPH0889177A - Bead for administering active ingredient for fishes - Google Patents

Abstract

PURPOSE: To obtain the beads capable of surely transporting various components effective to a fish body such as vitamin C to environmental water without scattering and losing and improving flesh and disease resistance in extremely high efficiency. CONSTITUTION: This beads for administering active ingredients for fishes contains active ingredients for fishes. The beads further can contain a feed attracting component. The active ingredients are various nutrient auxiliaries and/or medicines. Vitamins, highly unsaturated fatty acids and astaxanthin are cited as the nutrient auxiliaries. Antibiotics and vaccines are cited as the medicines. The active ingredients may be substances having low acid polymer. The main component of the beads is a natural polymer gelatinizing agent such as alginic acid, gelatin, pectin, chitosan and gum.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to beads for active ingredient administration for fish. INDUSTRIAL APPLICABILITY The present invention uses a natural polymer gelling agent as an outer membrane to form beads by incorporating active ingredients such as various nutritional supplements and drugs, or by mixing the gelling agent and active ingredients and then gelling them into beads. The present invention relates to beads for administering an active ingredient for fish.

[0002]

2. Description of the Related Art Improving the meat quality and disease resistance of cultured fish is a very important issue in the current aquaculture industry. Therefore, various nutritional supplements and drugs are routinely used,
Since an effective administration method has not been established, it causes eutrophication and eutrophication in the cultured sea surface and drug-resistant bacteria. Immediate improvement is desired.

As nutritional supplements, vitamins, polyunsaturated fatty acids, astaxanthin and the like, and as drugs, antibiotics and vaccines, etc., these are already on the market. There are various ways of using these, for example, vitamins, polyunsaturated fatty acids, and pigments are mixed in advance with the feed raw material.
It is often adsorbed and used in the field to produce a characteristic feed. When a fish disease occurs, the drug is adsorbed to the feed on site. As described above, the administration methods include mixing with feed, adsorption, injection into fish bodies, immersion in fish bodies, etc., but each has its own problems.

[0004]

Each administration method has the following problems. 1. When mixed and adsorbed in feed, the active ingredient will be dissipated in environmental water during feeding of fish. Also, feeding requires labor. 2. Injection into a fish body is very time-consuming and also gives high stress to the fish. 3. The effect is low when the fish is immersed, because the concentration of the drug is reduced and the drug is absorbed from the surface of the fish. Also, immersion in a cage or the like is not practical.

Therefore, an object of the present invention is to provide beads for administering an active ingredient for fish, which can minimize the dissipation into the environmental water and reliably transfer the active ingredient into the fish body. Further, the present invention aims to enhance the feeding ability and ensure the achievement of the above object.

[0006]

The present invention relates to a bead used for an oral administration method which is a very simple method of administering an active ingredient and has little stress on a fish body. The present invention is an active ingredient-administering bead for fish, which contains an active ingredient for fish. Alternatively, the present invention is a fish active ingredient-administering bead, which contains an active ingredient for fish and a feed attractant.

The active ingredient-administering beads for fish of the present invention are beads obtained by incorporating a natural polymer gelling agent into the outer membrane and incorporating active ingredients such as various nutritional supplements and drugs, or the gelling agent. And the active ingredient are mixed and then gelled to be beads. Since it is only necessary to orally administer the bead to the target fish directly in an optimum amount, there is no need for special equipment, feeding, or technology, and there is no stress on the target fish.

The natural polymer gelling agents include gelatin, pectin, chitosan, gum, alginic acid and the like. For gelatin, pectin, gum, etc., it is necessary to keep water warm when dissolving these substances in water. Since chitosan is soluble in acid, it must be dissolved in an aqueous solution of malic acid, acetic acid, etc., which is harmless to the living body, and this sour taste and odor reduce the palatability of fish, and the coagulation liquid currently contains dodecyl sulfate. There are many problems to be solved, such as having to use a substance harmful to the body called sodium.

Alginic acid is soluble in water without any treatment, and the coagulating liquid is safe for the living body because it uses calcium chloride as a food additive. As described above, alginic acid is preferable as the natural polymer gelling agent in view of the technical aspects of producing beads. In making the beads of the present invention, in the case of alginic acid, 0.5-2% wt, preferably 1.0-1.
A 5% wt gelling agent aqueous solution is used.

The active ingredients used for inclusion or kneading are various nutritional supplements and / or drugs effective for fish. Specifically, vegetable oils such as olive oil and soybean oil, animal oils such as sardine oil and cod liver oil, EP
A, highly unsaturated fatty acids such as DHA, astaxanthin,
Examples include pigments such as canthaxanthin, vitamins such as vitamin C and vitamin E, drugs such as antibiotics, vaccines and hormones. They may also be certain agents which should not be affected by gastric acid, such as hormonal agents, substances with low acid resistance such as vaccines.

Examples of the food intake-inducing component include krill meal, krill oil, oak mystic water, squid oil, squid meal, and squid liver powder.

To prepare the beads of the present invention, in the case of inclusion beads, a double nozzle is used, and the gelling agent is dropped from the outer nozzle and the active ingredient is dropped into the coagulating liquid from the inner nozzle to form beads. The size of the beads can be arbitrarily adjusted by changing the diameter of the dropping nozzle for the amount of the liquid dropped into the coagulating liquid. In the case of kneading type beads, a gelling agent and an active ingredient are mixed and dropped into a coagulating liquid from a nozzle to form beads. As the coagulating liquid, polyvalent metal salts, saccharides, sugar alcohols, organic acids, tannin and the like can be used, but in the case of alginic acid, calcium chloride is particularly effective. The particle size of the beads can be freely adjusted, and the particle size of the nozzle is selected. If smaller size beads are desired, a spray may be used. In the case of any of the beads, an emulsifier or the like is appropriately used in combination depending on the properties of the mixture.

[0013]

The details of the present invention will be described with reference to Examples. The present invention is not limited to these examples.

Example 1 (1) Production of Squid Liver Oil-Containing Beads First, sodium alginate was dissolved in distilled water with stirring while stirring, and 0.5 to 2.0 wt%, preferably 1.0.
An aqueous alginic acid solution of ˜1.5 wt% is prepared. Then, using a device as shown in FIG. 1, a double-structured droplet in which the outer membrane is alginic acid and the inside is squid liver oil was added dropwise to a 5% calcium chloride aqueous solution to coagulate to prepare beads containing squid liver oil.

(2) Administration of squid liver oil-containing beads Test fish: rainbow trout beads used: squid liver oil-containing beads The state in the stomach after administration of squid liver oil-containing beads was observed.
That is, squid liver oil-containing beads were administered to rainbow trout, and the number of squid liver oil-containing beads in the stomach after a certain time and the number of beads only in the outer membrane were measured, and the squid liver oil-containing beads ratio (number of included beads in stomach / The total number of beads in the stomach) was calculated to confirm the state of the squid liver oil-containing beads in the stomach. The results are shown in Table 1.

[0016]

[Table 1]

The squid liver oil-containing beads reached the stomach at a considerably high rate, suggesting that the active ingredient was less dissipated into the environmental water due to the fish chewing the beads during feeding.
Further, from FIG. 2, it was considered that although the beads remained as they were in the stomach, only the outer membrane of the beads remained in the intestine, and the contents were reliably released into the intestine.

(3) Elution of active ingredient in beads into environmental water Control group: Vitamin C-adsorbed dry feed Test group: Vitamin C kneaded beads Vitamin C-adsorbed dry feed, vitamin C and gelling agent were mixed The kneaded beads prepared as described above were prepared and allowed to stand in water for a certain period of time to compare the elution amount of vitamin C in water. The results are shown in Table 2 and FIG.

[0019]

[Table 2]

The amount of vitamin C contained in the beads dissolved in water is 1/2 or less that of the dry feed. This suggests that the burden of pollution on the cultured sea surface will be significantly reduced.

Example 2 The following experiment was conducted to confirm that the active ingredient in the beads reached the intestine. Test fish: Goldfish Beads used: Growth hormone (GH) -containing beads GH-containing beads were administered to goldfish, and it was confirmed that GH reached the intestine after a certain period of time. To confirm GH, intestinal contents were collected and subjected to Western blotting. The results are shown in Fig. 3.

From the results of Western blotting,
It was confirmed that GH reached the intestine of goldfish without fail, and the present invention was confirmed to be an effective method for transferring the active ingredient into the intestine of fish.

[0023]

EFFECTS OF THE INVENTION Various components effective for fish can be reliably transferred into the fish without being dispersed in environmental water. It can improve the meat quality and anti-disease property of fish with very high efficiency.

[Brief description of drawings]

1 is a drawing showing a method for producing squid liver oil-containing beads of Example 1. FIG.

FIG. 2 is an explanatory diagram showing a comparison of the amount of vitamin C eluted into water.

FIG. 3 is an explanatory view showing a state of growth hormone in the intestine after administration of growth hormone-containing beads to goldfish.

Continuation of the front page (51) Int.Cl. ⁶ Identification number Office reference number FI technical display location A23K 1/165 B 8502-2B 1/17 8502-2B A61K 9/16 X

Claims (6)

[Claims] 1. A bead for administering an active ingredient for fish, which comprises an active ingredient for fish. 2. The beads for administering an active ingredient for fish according to claim 1, which further comprises a feed attracting ingredient. 3. The beads for administering active ingredient for fish according to claim 1 or 2, wherein the active ingredient is various nutritional supplements and / or drugs. 4. The beads for administration of active ingredient for fish according to claim 1, wherein the active ingredient is a substance having low acid resistance. 5. The beads for administering an active ingredient for fish according to claim 1, wherein a main component of the beads is a natural polymer gelling agent. 6. The active ingredient administration beads for fish according to claim 5, wherein the natural polymer gelling agent is alginic acid.