JPH08310937A - Preparation for external use - Google Patents

Abstract

PURPOSE: To obtain a preparation for external use excellent in beautifying and whitening effects on the skin and humectant effects on the skin and hair. CONSTITUTION: This preparation for external use contains 0.0001-30wt.%, preferably 0.1-5wt.% neoagarobiose which is one of heterobioses. The neoagarobiose has high suppressing actions on melanogenesis and is excellent humectant actions. The neoagarobiose is further suitably blended with ingredients usually used in cosmetics, quasi-drugs or medicines and a well-known medicine, as necessary, can be added thereto to prepare the neoagarobiose into a skin-care cosmetic, a makeup cosmetic, a detergent, a cosmetic for hair and a dermal preparation for external use. The neoagarobiose is obtained by culturing Alteromonas sp. E-1 which is one of microorganisms capable of producing an agar hydrolase, providing the agar hydrolase, then hydrolyzing agar or agarose therewith and cooling and freeze-drying the resultant hydrolyzate.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to an external preparation containing neo-agarobiose. More specifically, by containing neo-agarobiose, it has an excellent whitening effect on the skin and an excellent effect on the skin and hair. And an external preparation having a moisturizing effect.

[0002]

2. Description of the Related Art Conventionally, as an external preparation for obtaining cosmetic effects such as preventing skin darkening, dullness, spots, freckles, etc., ascorbic acid or its derivative, glutathione, colloidal sulfur, placenta extract, and so on. Whitening cosmetics and the like containing an extract, mulberry skin extract, aloe extract and the like as active ingredients are known. Further, in order to improve skin roughness due to dryness, dryness of hair, etc., skin cosmetics, hair cosmetics, external skin preparations, etc. containing glycerin, urea, amino acids or their derivatives, hyaluronic acid, etc. as moisturizers are known. ing.

[0003]

However, in whitening cosmetics and the like, ascorbic acid itself is easily oxidized, so that it is difficult to expect a certain effect to be exhibited over time.
The cosmetics and the like containing this also have a drawback that they are easily discolored. In addition, ascorbic acid derivative, placenta extract, toki extract, mulberry bark extract, aloe extract are not said to be sufficiently effective alone, and glutathione and colloidal sulfur produce a peculiar offensive odor after blending. However, there is a drawback that precipitation may occur depending on the dosage form. On the other hand, glycerin, urea, amino acids or their derivatives, hyaluronic acid can also be used as an external preparation having a moisturizing effect such as preventing dryness of skin to improve rough skin, and preventing dryness of hair to improve dryness of hair. When a conventional moisturizer is used alone, increasing the blending amount to obtain a sufficiently high effect may impair the usability of the product. Under these circumstances, it has been desired to develop an external preparation which is more stable and has an excellent whitening effect on the skin and an excellent moisturizing effect on the skin and hair.

[0004]

In view of the above circumstances, the present inventors have diligently studied the whitening effect, the moisturizing effect and the like of saccharides, and as a result, have found that neo-agarobiose has a high melanin production inhibitory effect and an excellent moisturizing effect. In addition, the inventors have found that an external preparation containing the same exhibits excellent properties in skin whitening effect and skin and hair moisturizing effect, and completed the present invention.

That is, the present invention is an external preparation characterized by containing neo-agarobiose.

Neoagarobiose used in the external preparation of the present invention is one kind of heterodisaccharide, and its preparation method is not particularly limited. For example, as shown below, a specific agar-degrading enzyme derived from a microorganism is used. A method of decomposing agar or agarose with the use of and preparing.

First, Alteromonas sp. E-1 which is one of the agar-degrading enzyme-producing bacteria is cultured according to a conventional method,
Agar-degrading enzyme is obtained from the culture solution. Next, agar or agarose was added to 10 mM Tris-hydrochloric acid buffer solution (pH 7.5) so as to have a concentration of 0.5%, and the agar-degrading enzyme was added thereto at a concentration of 50 mU / ml or more, It is allowed to act at 40 ° C for 4 hours or more. The reaction is stopped by leaving this solution in boiling water for 5 minutes to stop the reaction at 4 ° C.
Cool to. Here, especially when agar is used as the substrate, insoluble matter precipitates and the like, so the residue is removed by centrifugation. Since the obtained supernatant contains neo-agarobiose, it can be lyophilized to obtain neo-agarobiose.

In the external preparation of the present invention, the content of neoagarobiose is preferably 0.0001 to 30% by weight (hereinafter,% by weight is simply represented by% unless otherwise specified), and particularly preferably The range of 0.01 to 5% is more preferable.

The external preparations of the present invention include, in addition to the above neoagarobiose, purified water, pH adjusting agents, lower alcohols, polyhydric alcohols, oils, powders, and interfaces commonly used for cosmetics, quasi drugs, and pharmaceuticals. Prepared by appropriately blending an activator, a water-soluble polymer and the like, if necessary, and in a quantitative and qualitative range that does not impair the effects of the present invention depending on the purpose, an emulsification aid, a preservative, a fragrance, A buffer solution, a dye and the like can be added.

If necessary, known agents may be added to the external preparation of the present invention. Examples of the drug include a drug having a whitening effect such as ascorbic acid or a derivative thereof, a placenta extract, a toki extract, a mulberry extract, an aloe extract; a moisturizing effect of glycerin, urea, an amino acid or a derivative thereof, hyaluronic acid and the like. Anti-inflammatory agents such as glycyrrhetinic acid or its derivatives and indomethacin; UV absorbers such as benzophenone, para-aminobenzoic acid, cinnamic acid or their respective derivatives; Antioxidants such as vitamin E, rosemary extract and tea extract Etc. These agents may be used alone or in combination of two or more kinds.

The dosage form of the external preparation of the present invention is not particularly limited,
Skin care cosmetics such as lotions, emulsions, creams, packs, cleansing agents, massage agents, beauty essences; makeup cosmetics such as foundations, lipsticks and blushers; facial cleanser,
Various dosage forms can be used, such as detergents for shampoos, body soaps, cosmetics for hair such as rinses and hair packs, external preparations for skin such as ointments and dispersions.

[0012]

EXAMPLES The present invention will be described in more detail below with reference to test examples and examples, but these do not limit the present invention in any way.

Test Example 1 Melanin Production Inhibition Test An appropriate amount of the medium was placed in two 6-well petri dishes, and B16 melanoma was inoculated and allowed to stand at 37 ° C. in a carbon dioxide concentration of 5%. The next day, the sample preparation solution is added and mixed so that the final concentrations of neoagarobiose (Note 1) are 0 (control), 1, 10, and 100 μg / ml, respectively. On the 5th day of culture, the medium is exchanged and the sample preparation solution is added again. The next day, remove the medium and
Cells (PBS (phosphate buffer solution))
After washing in, it was collected and the whitening degree of B16 melanoma was evaluated. (Note 1: Prepared by the method exemplified in this specification)

The evaluation criteria of whitening degree are as follows. ++: It became whiter than when 100 μg / ml of placenta extract was added. +: Whitened to the same extent as when 100 μg / ml of placenta extract was added. ±: Not whiter than when 100 μg / ml of placenta extract was added, but whiter than the control. -: Similar to the control.

For the remaining one dish, cells are fixed with formalin, and a 1% crystal violet solution is added for staining. Number of viable cells for each sample concentration (A)
And the cell number (B) of the control was measured, and the cell viability was calculated by the formula (1). Table 1 shows the above results.

[0016]

(Equation 1)

[0017]

[Table 1]

As is clear from the results shown in Table 1, it was confirmed that neo-agarobiose used in the present invention has low cytotoxicity to B16 melanoma and has a high melanin production suppressing ability. This indicates that neo agarobiose has a high whitening effect on the skin.

Test Example 2 Moisture Retention Test For each of the four moisturizers and neo agarobiose (the same as in Note 1) shown in Table 2, 0.1 g of each sample was precisely weighed and 40
Let stand for 3 days in a thermo-hygrostat at 75 ° C. The weight at this time was measured, and the moisturizing property was evaluated by the weight increase rate at this time.

[0020]

[Table 2]

As is clear from the results shown in Table 2, neoagarobiose used in the present invention has a higher hygroscopicity and an excellent moisturizing effect than other moisturizers.

Example 1 and Comparative Example 1 Emulsion An emulsion was prepared according to the composition and the production method shown below, and a usage test was conducted to evaluate the whitening effect and the rough skin improving effect due to the moisturizing effect according to the following evaluation methods and evaluation criteria. The results are shown in Table 3.

<Example 1> (Composition) (Component) (% by weight) (1) Squalane 5 (2) Vaseline 2 (3) Beeswax 0.5 (4) Sorbitan sesquioleate 0.8 (5) Poly Oxyethylene oleyl ether (20 EO) 1.2 (6) 1,3-butylene glycol 5 (7) Neoagarobiose (same as Note 1) 2 (8) Ethyl alcohol 5 (9) Preservative Suitable Quantity (10) Perfume Suitable quantity (11) Sodium hydroxide 0.1 (12) Carboxyvinyl polymer (1% aqueous solution) 20 (13) Purified water Residual quantity

(Production Method) A. Ingredients (6)-(8), (12) and (13) are heat mixed and maintained at 70 ° C. B. The components (1) to (5) and (9) are heated and mixed, and
Keep at 0 ° C. C. After A and component (10) are added to B and mixed uniformly, (11) is added to uniformly emulsify and cool to 30 ° C. to obtain an emulsion.

<Comparative Example 1> The composition is the composition of Example 1 except that neoagarobiose is removed, and the same amount of purified water is added instead. The manufacturing method is based on the manufacturing method of Example 1.

(Evaluation method) Twenty women aged 30 to 50 years were used as a panel, and two emulsions of Example 1 and Comparative Example 1 were used for 2 weeks, twice daily in the morning and night, and after washing the face, an appropriate amount was applied to the face. I got it applied to. The whitening effect and the rough skin improving effect by application were evaluated according to the following evaluation criteria.

(Evaluation Criteria) [Whitening Effect] Effective: Spots, freckles, and dullness were almost inconspicuous. Slightly effective: Spots, freckles, and dullness are less noticeable. No effect: No change [Effect of improving rough skin] Effective: Improves the roughness and roughness of the skin. Slightly effective: The bulkiness and roughness of the skin were slightly improved. Ineffective: Does not change

[0028]

[Table 3]

As is clear from the results of Table 3, Example 1
Compared with the emulsion of Comparative Example 1, good results were obtained in the removal of spots, freckles, dullness and improvement of skin roughness and roughness, and the emulsion of Example 1 showed an excellent effect of whitening effect and rough skin improving effect by moisturizing.

Example 2 and Comparative Example 2 Cream A cream was prepared by the composition and production method shown below, and its whitening effect was evaluated by a usage test. The evaluation method and evaluation criteria are the same as those in Example 1 and Comparative Example 1. The results are shown in Table 4.

<Example 2> (Composition) (Component) (% by weight) (1) Beeswax 6 (2) Cetanol 5 (3) Reduced lanolin 8 (4) Squalane 1.5 (5) Glycerin monostearate 0 .5 (6) Lipophilic glyceryl monostearate 13 (7) Polyoxyethylene sorbitan monolaurate (20 EO) 2 (8) Neo agarobiose (same as Note 1) 2 (9) Preservative 0.1 (10) Perfume proper amount (11) 1,3-butylene glycol 5 (12) Purified water balance

(Production Method) A. The components (1) to (7), (9) and (10) are mixed by heating and kept at 70 ° C. B. Ingredients (8), (11) and (12) are heat mixed and maintained at 70 ° C. C. Add A to B to emulsify and cool to 30 ° C. to obtain a cream.

<Comparative Example 2> The composition is the same as that of Example 2 except that neoagarobiose is removed, and the same amount of purified water is added instead. The manufacturing method is based on the manufacturing method of Example 2.

[0034]

[Table 4]

As is clear from the results shown in Table 4, Example 2
Compared to the cream of Comparative Example 2, the cream of spots, freckles,
Good results were obtained in eliminating dullness, and an excellent whitening effect was exhibited.

Example 3 and Comparative Example 3 Lotion A lotion was prepared by the composition and production method shown below, and the effect of moisturizing skin roughening was evaluated by a use test.
The evaluation method and evaluation criteria are the same as those in Example 1 and Comparative Example 1. The results are shown in Table 5.

<Example 3> (Composition) (Component) (wt%) (1) Glycerin 2.5 (2) 1,3-butylene glycol 3.5 (3) Oleyl alcohol 0.1 (4) Poly Oxyethylene sorbitan monolaurate (20EO) 1.5 (5) Polyoxyethylene lauryl ether (20EO) 0.5 (6) Ethyl alcohol 13 (7) Sorbitol 1 (8) Neoagarobiose (Same as Note 1) 0.01 (9) Vitamin E 0.1 (10) Oxybenzone 0.2 (11) Preservative Suitable amount (12) Perfume Suitable amount (13) Purified water Residual amount

(Production Method) A. Components (3) to (6) and (9) to (12) are mixed and dissolved. B. Components (1), (2), (7), (8) and (1
Mix and dissolve 3). C. Mix A and B to make uniform and obtain lotion.

<Comparative Example 3> The composition is the same as that of Example 3, except that neoagarobiose was removed, and the same amount of purified water was added instead. The manufacturing method is based on the manufacturing method of Example 3.

[0040]

[Table 5]

As is clear from the results in Table 5, Example 3
As compared with Comparative Example 3, the lotion of No. 3 gave good results in improving the bulkiness and roughness of the skin, and showed an excellent effect of improving skin roughness by moisturizing.

Example 4 and Comparative Example 4 Hair Shampoo A hair shampoo was prepared according to the composition and production method shown below, and a usage test was conducted to evaluate the moisturizing effect of hair according to the following evaluation methods and evaluation criteria. The results are shown in Table 6.

<Example 4> (Composition) (Component) (% by weight) (1) Lauryl sulfate triethanolamine 16 (2) Lauric acid diethanolamide 4 (3) Propylene glycol 2 (4) Preservative proper amount ( 5) Appropriate amount of fragrance (6) Neo agarobiose (same as Note 1) 1.5 (7) Residual amount of purified water

(Production Method) A. Components (1) to (5) are mixed and dissolved. B. Components (6) and (7) are mixed and dissolved. C. Add B to A and mix evenly to obtain a hair shampoo.

<Comparative Example 4> The composition is the composition of Example 4 except that neoagarobiose is removed, and the same amount of purified water is added instead. The manufacturing method is based on the manufacturing method of Example 4.

(Evaluation method) Hair shampoo 2 of Example 4 and Comparative Example 4 with 20 women aged 25 to 45 as panels
The product was washed once a day for 5 days. The moisturizing effect of hair washing was evaluated according to the following evaluation criteria.

(Evaluation Criteria) [Hair Moisturizing Effect] Effectiveness: The moist feeling of the hair was increased and the hair became smooth. Slightly effective: The moist feeling of the hair was slightly increased, and the hair became slightly smooth. Ineffective: Does not change

[0048]

[Table 6]

As is clear from the results of Table 6, Example 4
The hair shampoo of No. 3 gave better results in imparting a moisturizing feeling to the hair and showed an excellent moisturizing effect on hair, as compared with the hair shampoo of Comparative Example 4.

Example 5 Pack A pack was prepared with the following composition and production method. <Example 5> (Composition) (Component) (% by weight) (1) Polyvinyl alcohol 15 (2) Sodium carboxymethyl cellulose 5 (3) Propylene glycol 3 (4) Neo agarobiose (the same as Note 1) 5 (5) Ethyl alcohol 10 (6) Oxybenzone 0.1 (7) Preservative proper amount (8) Perfume proper amount (9) Purified water balance

(Production Method) A. Components (1) to (4) and (9) are mixed and dissolved by heating at 70 ° C. with stirring. B. Components (5) to (7) and (8) are mixed and dissolved. C. B is added to A, mixed uniformly, and then cooled to obtain a pack.

The pack of Example 5 obtained as described above was used to eliminate spots, freckles, dullness, and skin roughness.
Good results were obtained in improving roughness.

[0053]

As described above in detail, the external preparation of the present invention exhibits excellent properties in whitening the skin and moisturizing the skin and hair.

Continuation of front page (51) Int.Cl. ⁶ Identification code Office reference number FI Technical display area A61K 7/075 A61K 7/075 31/70 ADA 31/70 ADA

Claims (3)

[Claims] 1. An external preparation characterized by containing neo-agarobiose. 2. The external preparation according to claim 1, which contains neo-agarobiose as a whitening agent. 3. The external preparation according to claim 1, which contains neo-agarobiose as a moisturizing agent.