JPH08169801A - Oxygen addition device for organ preservation device - Google Patents

Abstract

(57) [Summary] [Structure] Liquid inlet / outlet 6, 6'and gas inlet /
Two bundles of semipermeable hollow fibers are packed in a container 4 having outlets 5 and 5 ', and both ends of the two bundles of semipermeable hollow fibers are fixed by fixing members 3 and 3'which are liquid tightly adhered to the inner wall of the container. An oxygen adding device for organ preservation device in which both ends of the semipermeable hollow fiber 2 are in communication with the inlets / outlets 5, 5 ′, 6, 6 ′ of the liquid (or gas) while being kept open. [Effect] With a large oxygen transfer amount to the perfusate and a small oxygenator, the excised organ can be preserved in a good condition for a longer period of time, and the survival rate after transplantation can be expected to improve.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to an oxygenator for an organ preservation device. More specifically, until transplanting the organ removed from the human body to the donor,
In an organ preservation device used for preservation or transportation, the present invention relates to an oxygen addition device that sufficiently supplies oxygen to reflux liquid to an organ.

[0002]

2. Description of the Related Art A simple cold preservation method is available to preserve an excised organ. This is simply storing the organ in a container in a frozen or cold state, but this method limits the storage period of the organ.

For this reason, a method called a low temperature reflux storage method is used. In addition to the perfusion chamber, the structure of this device consists of an oxygen addition device, a heat exchanger, a liquid feed pump, and
It has a monitor and control device for perfusion pressure and flow rate.

As an example of the perfusate sent to the perfusion chamber, a liquid composed of plasma (CPP) / magnesium sulfate / dextrose / insulin / penicillin / hydrocortisone and a plasma protein portion (PPF) by a cryoprecipitation method.
There is an albumin base solution.

When storing an organ excised from a living body for a long period of time, it is necessary to supply sufficient oxygen to the cell tissues that compose the organ. In the case of microorganisms, air is added to the preservation solution with a member having pores. The presence of microbubbles is also allowed, but when supplying oxygen to the organ, it is not preferable that the air bubbles come into direct contact with the organ. With that in mind, oxygen must be dissolved in the perfusate. Therefore, there is an oxygen addition device of a type in which air bubbles do not occur.

For example, the inside diameter is 2-3 mm in the housing.
Then, put a silicon tube with an outer diameter of 3 to 3.5 mm and a length of 1 to 2 m, flow a perfusion solution into the tube, and put oxygen of 95% purity or the atmosphere into the outside of the tube to add oxygen to the perfusion solution. Had been dissolved. Silicon is used in this case because of its high gas permeability.

[0007]

However, the silicon tube is not sufficient for a small-sized oxygen addition device in which a large amount of oxygen is transferred and which is small in size. The present invention has been made to solve this problem.

[0008]

SUMMARY OF THE INVENTION The present invention is directed to a liquid inlet
The semipermeable hollow fiber bundle is packed in a container having an outlet and a gas inlet / outlet, and both ends of the semipermeable hollow fiber bundle are fixed by fixing members that are liquid-tightly adhered to the inner wall of the container. An oxygen adding device for organ preservation device is characterized in that both ends thereof are communicated with an inlet / outlet of the liquid (or gas) while being kept open.

In the present invention, the material of the container (a cap is also used if necessary) is polyolefin, polycarbonate, polyacrylate, polyacrylonitrile,
Examples include polystyrene and polyvinyl chloride. Also,
The shape of the container is preferably cylindrical for manufacturing. In this case, fluid inlets and outlets are provided at both ends of the container. However, it is not necessary to stick the shape of the container into a tubular shape, and for example, the hollow fiber bundle may be folded and stored in the container.

In the present invention, the semipermeable hollow fiber has a cylindrical porous membrane, fine pores, and is made of polyamide, polyester, polyvinyl alcohol, polycarbonate, polyolefin, polysulfone, polyacrylate, etc. used. The morphological example of the semipermeable hollow fiber has a membrane having a pore diameter of 0.05 to 0.1 μm and an inner diameter of 100 to 100.
The thickness is 0 μm and the film thickness is 10 to 50 μm. The reason why the porous membrane as described above is used in the form of a hollow fiber is that not only can the gas-liquid distance be shortened, but also the gas-liquid contact area (this is called the effective membrane area) can be increased. Also, it is easy to manufacture the device.

In the present invention, the reason why the inner wall of the container and the fixing member are in liquid tight contact is to separate the gas and the perfusate. In the present invention, examples of the material of the fixing member include epoxy resin, polyurethane and silicone resin.

When the oxygenator of the present invention is used, a perfusate may be passed through the hollow fiber to allow air or oxygen to flow outside the hollow fiber, and conversely, air or oxygen may be passed through the hollow fiber to remove the hollow fiber. The perfusate may be flown outside. In any case, it is necessary to keep air or oxygen at a predetermined pressure.

[0013]

When the oxygenator of the present invention is used, air or oxygen is transferred from the pores of the semipermeable membrane to the perfusate, but since the pore size is such that bubbles do not occur, the bubbles are the perfusate. There is no danger of being mixed in. In addition, since the membrane is a bundle of thin hollow fibers, the effective membrane area becomes large, and air or oxygen can be efficiently transferred to the perfusate, and the dissolved amount of oxygen in the perfusate reaches the saturation point even if atmospheric air is used. be able to.

[0014]

EFFECTS OF THE INVENTION Since the present invention has the above-mentioned structure, it is possible to obtain a small oxygen addition device in which the amount of oxygen transferred to the perfusate is large. As a result, it is expected that the removed organ can be preserved in a good condition for a longer period of time and the survival rate after transplantation can be improved by the oxygen impermeability device of the present invention.

[0015]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below with reference to the drawings. FIG. 1 is a cross-sectional view showing an example of the oxygen adding device for an organ preservation device of the present invention, as viewed from the side.

In the drawing, caps 7 ', 7 having fluid inlets, outlets 6, 6'are fitted on both ends of an outer cylinder 4 having fluid inlets 5, 5'to form a housing 1 made entirely of polycarbonate. There is.

Semipermeable hollow fibers 2 are packed in an outer cylinder 4 at a position parallel to the longitudinal direction of the outer cylinder. Both ends of the fiber 2 are fixed in the outer cylinder 4 by fixing members 3, 3'of polyurethane. The fixing members 3 and 3 ′ are in close contact with the inner wall of the outer cylinder 4. The tips of the filaments of the hollow fiber 2 are kept open and communicate with the fluid inlets and outlets 6, 6 '.

When dissolving oxygen in the perfusate, the perfusate is introduced from the fluid inlet 6, passes through the inside of the hollow fiber 2, and is taken out from the fluid outlet 6 '. On the other hand, air or oxygen is fed from the fluid inlet 5 at a predetermined pressure, and the air or oxygen passes through the outside of the hollow fiber 2 and is taken out from the fluid outlet 5 ′.

The following experiments were conducted to see the effects of the present invention. Approximately 7,000 acetate hollow fibers with an outer diameter of 200 μm and a film thickness of 15 μm (effective membrane area: 0.7 square meters)
Both ends of are fixed in the housing to make one module, the distilled water deoxygenated by nitrogen substitution is passed through the hollow fiber, and the atmosphere is ventilated outside the hollow fiber at a flow rate of 0.6 l / min. When the flow rate is 20 to 200 ml / min, the oxygen partial pressure of distilled water at the module outlet is 180 m
It became mHg (saturation point), and sufficient substitution was possible.

On the contrary, the same result was obtained by flowing the air into the hollow fiber and flowing the water out of the hollow fiber.

On the other hand, similar results were obtained when the above experiment was carried out by replacing the distilled water with a liquid containing CPP, magnesium sulfate, dextrose, insulin, penicillin, and hydrocortisone.

For comparison, the inner diameter is 2 m in the housing.
m, an outer diameter of 3 mm, and a length of 1.5 m are put into the tube, distilled water deoxygenated by nitrogen substitution is passed through the tube, and the atmosphere is vented to the outside of the tube at a flow rate of 0.6 l / min. When the flow rate of distilled water was 20 to 200 ml / min after the addition, the oxygen partial pressure of distilled water at the module outlet was 140 mmHg, which was inferior to that of the example.

Furthermore, as a result of storing the liver of the mouse isolated with a PPF-based perfusate, it was possible to store it for 3 to 4 days when aerated with the above-mentioned oxygenator for silicon. When aerated with the device, it was possible to store for more than one week.

[Brief description of drawings]

FIG. 1 is a cross-sectional view showing an example of an oxygen adding device for an organ preservation device of the present invention, as viewed from the side.

[Explanation of symbols]

 1 Housing 2 Hollow Fiber 3 Fixing Member 4 Outer Cylinder 5 Fluid Port 6 Fluid Port 7 Cap

Claims (2)

[Claims] 1. A semipermeable hollow fiber bundle is packed in a container having a liquid inlet / outlet and a gas inlet / outlet, and both ends of the semipermeable hollow fiber bundle are fixed to a container inner wall in liquid tight contact with each other. An oxygen adding device for organ preservation device, characterized in that the semipermeable hollow fiber is fixed and communicated with the inlet and the outlet of the liquid while keeping both ends open. 2. A semipermeable hollow fiber bundle is packed in a container having a liquid inlet / outlet and a gas inlet / outlet, and both ends of the semipermeable hollow fiber bundle are fixed to a container inner wall in a liquid-tight manner by a fixing member. An oxygen adding device for organ preservation device, characterized in that both ends of the semipermeable hollow fiber are fixed and communicated with the gas inlet / outlet while being kept open.