JPH08146007A - Chemical analyzer - Google Patents

Abstract

(57) [Summary] [Purpose] To prevent carryover of chemical analysis equipment and to reduce the overall size of the equipment. [Structure] Agitating the inside of a reaction vessel 301 for reacting a sample with a reagent does not use a spatula or a screw, but uses an acoustic rectilinear flow generated by ultrasonic waves, so that the sample and the reagent inside are not in contact with each other. Stir.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a chemical analysis device,
In particular, it relates to stirring of samples and reagents.

[0002]

2. Description of the Related Art Conventionally, as a chemical analyzer, there is a chemical analyzer described in US Pat. No. 4,451,433. This device consists of a colorimetric measuring unit for analyzing and quantifying proteins and ions in blood, components in urine, and an ion analyzing unit for analyzing ions in blood. Therefore, it has a processing speed of 9000 tests or more when it comes to large equipment. In particular, in order to increase the processing speed in the colorimetric measurement unit, a large number of reaction vessels are provided on the circumference of the turntable on the upper surface of the main body of the chemical analyzer, and a system for sequentially mixing, reacting and measuring samples by overlap processing. Is. The main components of this device are the sample, the automatic sample / reagent supply mechanism for supplying reagents to the reaction container, the automatic stirring mechanism for stirring the sample / reagent in the reaction container, and the sample during or after the reaction. It has a measuring instrument for measuring the physical properties of the above, an automatic cleaning mechanism for sucking and discharging the sample for which the measurement has been completed and cleaning the reaction container, a control section for controlling these operations, and the like. An automatic stirring mechanism for stirring the sample and the reagent is connected to a spatula for descending below the liquid surface in the reaction vessel to cause a swirling flow, and the root of the spatula,
It is composed of a motor for rotating the spatula, a cleaning container for cleaning the spatula, and a drive mechanism for moving back and forth between the cleaning container and the reaction container.

[0003]

In the field of chemical / medical analysis, reducing the amount of liquid such as samples and reagents has become a major technical problem. That is, as the number of analysis items increases,
DNA that cannot be prepared in large quantities due to the small amount of sample that can be assigned to a single item and the sample itself being precious
The analysis using a small amount of sample or reagent, which has been conventionally regarded as a sophisticated analysis, is now routinely performed. Further, as the contents of analysis have become more sophisticated, expensive reagents have come to be generally used, and there is a demand for reducing the amount of reagents in terms of running costs. or,
In the fields of drug manufacturing and biotechnology, preparation of trace reagents and preparation of samples using trace substances are becoming important.

In the above-mentioned prior art, the stirring for mixing the liquids inside the container is performed by using a spatula or a screw. At this time, there is a problem that the liquid after stirring adheres to a spatula or a screw and is carried over to the inspection of the next sample, and the next sample or sample is contaminated, which affects the analysis result.

Further, in recent years, various devices are being introduced into hospitals and the like in which such a chemical analyzer is installed, and further miniaturization is desired.

An object of the present invention is to provide a chemical analysis device,
The purpose is to prevent carryover when the sample inside the container is mixed with the sample.

Another object of the present invention is to reduce the size of the chemical analyzer itself.

[0008]

The former purpose is to provide a reaction container, sample supply means for supplying a sample from the upper opening of the reaction container, and reagent supply for supplying a reagent from the upper opening of the reaction container. In the chemical analyzer comprising a means and a measuring means for measuring the physical properties of the sample during or after the reaction, a sound wave generating means is provided outside the reaction container and generates a sound wave toward the reaction container. It is achieved by providing.

The latter purpose is to provide a turntable in which a plurality of reaction vessels are arranged on the circumference, a sample turntable in which a plurality of sample vessels containing samples are arranged on the circumference, and a reagent. A plurality of reagent containers arranged on the circumference of the reagent turntable, sample supply means for supplying the sample in the sample container from the upper opening of the reaction container, and the reagent in the reagent container In a chemical analyzer equipped with a reagent supply means for supplying from an upper opening of a reaction container and a measurement means for measuring the physical properties of the sample during or after the reaction, the reaction container is provided outside the reaction container. This is achieved by providing a sound wave generating means for generating a sound wave toward.

[0010]

In the former case, the main cause of carry-over is that the sample and the reagent injected into the reaction vessel are mechanically stirred by a spatula or a screw. In the present invention, the sound wave generated by the sound generating means provided outside the reaction container is applied to the reaction container. This sound wave acts so as to stir the sample and the reagent inside the container via the reaction container. Therefore, in the chemical analyzer, it is not necessary to mechanically stir with a spatula or a screw, and carry-over can be prevented.

On the other hand, with regard to the latter, the structure which controls the size of the chemical analyzer itself is mainly composed of a turntable for accommodating a plurality of reaction vessels, a turntable for samples accommodating a plurality of sample vessels, and a plurality of turntables for samples. It is the diameter of the reagent turntable that houses the reagent container.

The diameter of these turntables is a value determined by the size of each container and the number of stored containers. Since it is difficult to reduce the number of measurements per unit time from the viewpoint of throughput, it is necessary to downsize each container. However, the size of the individual containers is dictated by the amount of sample and reagents contained in the container, so that the container can no longer be made smaller.

By the way, the amount of the sample and the reagent to be put in the container is not determined by the amount necessary for analysis and measurement, but is determined by the amount which can be mechanically stirred by a spatula or a screw. That is, when the amounts of the sample and the reagent are small, it becomes difficult to stir, and therefore the amount is set to be more than the amount required for analysis / measurement.

In the present invention, the stirring is not performed mechanically, but is performed by contact with the object to be stirred inside the container by means of sound waves. In the case of the mechanical type, the amount of the object to be stirred is required to some extent in order to physically mix the object to be stirred with a spatula or the like, but in the present invention, the object to be stirred is agitated by using a sound wave. Since it stirs itself by the flow, even a small amount of the object to be stirred can be mixed well.

From this, the amount of sample and reagent is
The amount required for analysis and measurement is sufficient, and individual reaction vessels can be made smaller, and as a result, the turntable diameter can be made smaller.

If the amounts of the sample and the reagent to be injected into the reaction container can be reduced as described above, the sample amount and the reagent amount can be reduced, and therefore the sample container and the reagent container can be reduced in size. The diameter of the turntable can be reduced.

Since the diameter of these turntables can be reduced, the overall size of the chemical analyzer can be reduced as a result.

[0018]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described with reference to FIGS. FIG. 1 is a perspective view showing the configuration of the chemical analysis device of the present embodiment, and FIG. 2 is a vertical cross-sectional view showing the configuration of a non-invasive (non-contact) stirring device equipped in the chemical analysis device shown in FIG.

A plurality of reaction vessels 301 are fixed on the circumference of the vessel fixed turntable 302. The container fixed turntable 302 is rotated in the circumferential direction by a table drive unit 303 including a motor, a rotary shaft, and the like (not shown). On the other hand, the sample is multiple sample cups 305.
These sample cups 305 are fixed by the table drive unit 307 on the circumference of the sample turntable 306 which rotates in the circumferential direction once per rotation. The sample automatic pipetting mechanism 304 supplies the sample in the sample cup 305 sent to a fixed position to the reaction container 301 as the sample turntable 306 rotates in accordance with a predetermined sequence.
In addition, the reagents are contained in a plurality of reagent bottles 309, and these reagent bottles 309 are fixed on the circumference of a reagent turntable 310 that rotates in the circumferential direction by a table drive unit 311. Also, the reagent in the reagent bottle 309 which has come to the fixed position is supplied to the reaction container 301 with the rotation of the reagent turntable 310 according to a predetermined sequence. The reagent bottles 309 contain different types of reagents, and the reagent automatic pipetting mechanism 308 supplies the required amount to the reaction container 301 in a predetermined sequence.

At the position where the reagent automatic pipetting mechanism 308 discharges the reagent into the reaction container 301, a non-invasive stirring device 312 and a driver 313 for driving and controlling the non-invasive stirring device 312 are provided. Then, the sample and the reagent in the reaction container 301 that have moved to that position are stirred and mixed. Further, in order to promote the chemical reaction between the sample and the reagent, all reaction vessels 301 are in the bath 40.
The reaction vessel 301 is immersed in the constant temperature water 402 in the No. 1 and moves in the constant temperature water 402.

At another position in the circumferential direction of the container fixed turntable 302, a measuring unit 314 for measuring the physical properties of the liquid reacted in the container with light is provided. At another position, a cleaning mechanism 315 for sucking the reaction liquid in the container, discharging the cleaning liquid, and cleaning the reaction container 301 moved to that position is provided. At the same position as the cleaning mechanism 315, a non-invasive stirring device 316 for stirring the cleaning liquid in the container by convection to enhance the cleaning effect, and a driver 317 for driving the non-invasive stirring device 316 are provided. Fixed container turntable 303, table driving units 307, 311 and sample automatic pipetting mechanism 304, reagent automatic pipetting mechanism 308, 2
The drivers 313, 317, the cleaning mechanism 315, and the measurement unit 314 for the two non-invasive agitation devices are connected to the control unit 318 via the control signal line, and the sample (this In this case, the blood to be inspected) is automatically analyzed and measured.

Next, a device for stirring the object to be stirred inside the reaction vessel in a non-contact manner will be described with reference to FIG. When the reaction container 301 repeats the rotational movement and stop, and stops at the position shown in the figure, the controller 313 (317, the controller 317 controls the cleaning stirring device 316, but the structure and operation thereof are non-invasive stirring devices for stirring. 313
Therefore, in this specification, the non-invasive stirring device 313 and the controller 313 will be described as representatives.
A signal is output from the piezoelectric element 103 to the piezoelectric element 103, and the piezoelectric element 103 outputs an ultrasonic wave having a high frequency as described later in detail. As described above, the non-invasive stirring device 312 (general term for the entire device shown in FIG. 2) is the reaction container 30 containing the solute (sample) 101 and the solvent (reagent) 102.
1 is fixed to the sample turntable 302. The reaction vessel 301 is a bath 40 containing constant temperature water 402.
It is immersed in 1. The piezoelectric element 1 is provided on the bottom of the bath 401.
03 is fixed to the lower portion of the bath 401 via the position adjusting unit 106, and is provided to the reaction vessel 301 via constant temperature water 402. The piezoelectric element 10 of the bath 401
A seal 107 is provided on the attachment portion of No. 3 to prevent the constant temperature water 402 from flowing out. Piezoelectric element 103
Are driven at a predetermined frequency by the controller 501.

The operation of the non-invasive (contact) stirring device 312 configured as described above will be described below. Piezoelectric element 103
Is vibrated at a predetermined frequency by the controller 501, and the generated vibration is generated in the bathtub 40 in the direction indicated by the arrow 105.
It propagates as a sound wave (indicated by an arrow 403) in 1 and reaches the bottom of the reaction container 301. This sound wave is generated by the reaction container 30.
1 Solvent that is an object to be agitated in the container by passing through the bottom wall surface 10
2 and solute 101 are reached.

The transmitted vibration wave propagates in the solvent vertically as a sound wave. A steady flow called an acoustic rectilinear flow indicated by an arrow 203 is generated in the propagation direction. The cause of this steady flow is described in the "Physical Acoustics" page of the literature.
As described in 265-330, when a sound wave propagates in a fluid such as a solvent in the vibration direction, absorption of the sound wave occurs due to the influence of the viscosity and volume viscosity of the fluid, and this absorption causes the sound wave to propagate in the propagation direction. It is believed that this is because the energy difference between the two causes a pressure gradient. By the acoustic rectilinear flow 203, the solute 101 is levitated vertically upward in the solvent 102, and after reaching the vicinity of the liquid surface, forms a vertical convection that descends the surroundings again.
Therefore, the solvent 102 and the solute 101 are stirred without inserting an insert such as a spatula into the container.
The flow velocity of the acoustic rectilinear flow 203 is the sound absorption coefficient of the solvent 102,
According to the experimental results, the condition that the acoustic rectilinear flow is remarkably generated in the reaction vessel 301 is that the vibration speed of the piezoelectric element 103 is at least 0.1 mm / s or more. I found it necessary.

This sound wave causes an acoustic rectilinear flow 203 in the fluid such as the solvent 102, promotes the movement of the solute 101, and agitates. In the figure, the reaction container 301 and the piezoelectric element 1
Although the constant temperature water 402 is interposed between the reaction vessel 301 and the reaction vessel 03, it can be stirred even if the reaction vessel 301 is brought into contact with the piezoelectric element 103.

As described above, according to the present embodiment shown in FIGS. 1 and 2, the solute and the solvent in the reaction vessel 301 are agitated without using a contact type agitation means such as a spatula. The object to be stirred attached to the spatula does not mix into another reaction container 301 (carry over) and affect the analysis / measurement results.

Further, there is a demand for downsizing the entire chemical analyzer, but in order to downsize, the diameter can be downsized by reducing the number of reaction vessels 301 mounted on the vessel fixed turntable 302. Although it can be achieved, it is not preferable because the throughput as a chemical analyzer decreases.
By the way, in the contact type stirring means, there is a problem that the amount of the object to be stirred cannot be agitated when the amount of the object to be agitated is equal to or lower than the lower limit value, and the amount is larger than the amount required for analysis and measurement. The volume had to be increased, and as a result, the diameter of the container fixed turntable 302 was increased. Further, since the amount of liquid necessary for stirring is sufficient, the amount of sample and reagent is large, and the sample cup 305 and the reagent bottle 309 are also large in size. Therefore, the sample turntable 30
4 and the reagent turntable 310 cannot be made smaller in diameter, which has hindered the downsizing of the entire chemical analyzer.

According to the present embodiment, since the stirring of the substance to be stirred in the reaction vessel is a non-contact type, the amount of the substance to be stirred (sample or reagent) can be set to the amount required for analysis / measurement. As a result, the reaction vessel 301 can be downsized, and accordingly, at least the diameter of the vessel fixed turntable 302 can be reduced. Therefore, the overall size of the apparatus can be reduced while maintaining the throughput of the chemical analysis apparatus. Furthermore, if miniaturization is desired, the amount of the sample and the reagent necessary for the reaction is also reduced, so that these containers can be miniaturized. As a result, the turntable 304 for the sample and the turntable 310 for the reagent can be downsized. Since the diameter can also be reduced, the overall size of the chemical analyzer can be reduced. In addition, if the size of the reagent bottle 309 of the reagent is left as it is and the amount of the reagent is set to the same amount as the conventional one, the amount of the reagent replacement work with respect to the number of times the sample is replaced is reduced even if the size of the device is somewhat sacrificed. There is an effect of doing.

By the way, a method described in the following documents is known as a method of mixing using ultrasonic waves, although it is not a chemical analyzer.

In Japanese Patent Laid-Open No. 57-28182, a liquid crystal and a polychromatic dye are mixed. Therefore, a container containing these liquids is capped, and then ultrasonic waves are applied to the container to vibrate the container at a high frequency. It is described to mix.

The Acoustical Society of Japan, Vol. 45, No. 1 (198
9) "Promotion of heat transfer by acoustic streaming" describes the use of a straight-ahead acoustic flow having the same effect as a forced flow from the outside in order to promote heat transfer of a heated object.

Further, 1991 IEEE pp. 277-282 "Ultrasonically Induced Microtransp
"ort" describes that a piezoelectric thin film is provided on the bottom wall surface of the tank as a fluid moving means to generate a transverse traveling wave.

Now, when the acoustic streaming is used as the stirring mechanism of the chemical analyzer, the following problems occur. That is, in a chemical analyzer, there is a step of first injecting a sample into a reaction container, then injecting a reagent and stirring, so that the upper portion of the reaction container must be open. At this time, it was found that there is a problem in that if ultrasonic waves are unnecessarily applied, the object to be stirred will blow out from the upper opening.

In the embodiment described below for solving this point, when the ultrasonic wave for generating the acoustic rectilinear flow is applied, the relative position between the piezoelectric element and the reaction container is made appropriate so that the object to be stirred is By not applying ultrasonic waves of the same intensity evenly to the above, it is possible to prevent the object to be stirred from moving to the upper part and blowing out at the same time. That is, it is intended to prevent blowout by causing a flow in the object to be stirred inside the container. Below, FIG.
It will be described with reference to FIGS. In these figures, the same symbols as those in FIGS. 1 and 2 indicate the same components.

3 is different from the embodiment shown in FIG. 2 in that the position of the piezoelectric element 103 is displaced from the center of the bottom of the reaction vessel 301. Since this position has an optimum position with respect to the reaction container stop position, some adjustment is required at the time of manufacturing. Therefore, the position adjusting unit 106 for adjusting the position of the piezoelectric element 103 attached to the bottom of the bath 401 is provided. It was Since the piezoelectric element 103 is arranged in this way, the ultrasonic wave generated by the piezoelectric element 103 propagates in the constant temperature water 401 as indicated by the arrow 105,
Upon reaching the bottom of the reaction vessel 301, an acoustic rectilinear flow 203 is generated inside the reaction vessel. The acoustic rectilinear flow 203 is generated near the wall surface of the reaction vessel 301 because the piezoelectric element 103 is offset from the center. Therefore, the object to be agitated inside rises in the vicinity of the wall surface along with the acoustic rectilinear flow 203 and descends from the vicinity of the opposite wall surface.
8 and promote internal stirring. In this way, circulating flow 1
The reason why 08 occurs is that a difference is provided in the energy intensity distribution in a plane perpendicular to the propagation direction of sound waves. In this case, the piezoelectric element 103 was arranged so that the energy intensity near the wall surface of the reaction vessel 301 was the highest. If the acoustic rectilinear flow 203 occurs in the ultrasonic wave propagation direction
Is uniform in a plane perpendicular to the propagation direction of sound waves, a relative velocity difference does not occur in the acoustic rectilinear flow 203 in the reaction vessel 301, so that not only a circulating flow does not occur, As described above, the object to be stirred spouts from the reaction container 301 through the upper opening.

According to this embodiment, there is an effect that the jetting can be prevented and the stirring can be efficiently performed in a short time.

Another embodiment will be described with reference to FIG. FIG.
The difference from the embodiment shown in FIG.
01 is provided on the side surface, not the bottom portion. Reaction vessel 3
Since ultrasonic waves are radiated obliquely (angle adjustment is performed by the angle adjusting unit 109) to a part (bottom surface and side surfaces) or all (side surfaces) of 01, the inside of the reaction vessel 301 is operated by the same action as above. An acoustic rectilinear flow 108 is generated which advances in an oblique direction toward the wall opposite to the irradiated wall. The flow impinging on the opposite wall becomes a circulating flow 108 that splits up and down and then returns due to the angle.

According to this embodiment, since the circulation flow is divided in this way, the vector of the upward flow becomes smaller than that in the embodiment shown in FIG. 3, and the possibility of ejecting the object to be stirred is reduced. In addition to the above effect, since the piezoelectric element is attached to the side surface of the bath 401, there is an effect that the angle can be easily adjusted during manufacturing.

Another embodiment will be described with reference to FIG. FIG.
3 is different from the embodiment shown in FIG. 3 in that the piezoelectric element 103 is displaced in the direction along the bottom of the bath 401 so that the ultrasonic wave irradiation position is displaced from the center of the bottom surface of the reaction vessel 301. However, in the present embodiment, the ultrasonic wave irradiation position is near the center of the bottom of the reaction vessel 301, and the piezoelectric element 1
03 and a bottom portion of the reaction container 301 are appropriately arranged to form a circulation flow inside the reaction container 301. The ultrasonic wave generated from the piezoelectric element 103 has a portion where the energy intensity is sharpened in a plane perpendicular to the propagation direction. By aligning the bottom of the reaction vessel 301 with the sharpened portion, that is, the position where the energy is converged, a sharpened acoustic rectilinear flow 203 is generated in the vicinity of the central portion inside the reaction vessel 301, thereby rising from the center, A circulating flow 108 is created which descends from the surroundings. This sharpened portion can be found by adjusting the piezoelectric element 103 in the vertical direction by the distance adjusting section.

According to this embodiment, the object to be agitated, which is particularly settled at the center of the bottom of the reaction vessel, can be aimed and floated, so that the agitation can be carried out in a short time.

FIG. 6 shows an embodiment in which the energy intensity is further sharpened in the embodiment shown in FIG. Piezoelectric element 1
The point that the acoustic lens 601 is provided in the radiation direction of the vibration wave of No. 03 is different from the example shown in FIG. The acoustic lens 601 has a function of converging the vibration wave, and the ultrasonic wave emitted from the acoustic lens 601 passes through the bottom of the reaction container 301 and is converged in the vicinity of the solvent 102 and the solute 101.
As a result, a strong sound field is formed vertically upward in the vicinity of the solute 101, so that an acoustic rectilinear flow 203 sufficient to levitate the solute 101 is generated. This is especially effective when a solute having a large specific gravity or viscosity or when rapid stirring is desired. Further, in the present embodiment, it is possible to operate as follows. The vibration wave radiated from the piezoelectric element 103 hits the solute 101 and is partially reflected. The piezoelectric element 103 detects this time, and the controller 313 identifies the position of the solute 10l. Acoustic lens 60
1 receives a position information signal indicating the position of the solute 101 from the controller 313, operates so as to converge the vibration wave to the position of the solute 101, and efficiently produces an acoustic rectilinear flow sufficient to levitate the solute 101. give.

The chemical analyzer of the type in which the turntable is rotated for automatic analysis has been described above.
An embodiment of a semi-automatic chemical analyzer having a small number of reaction vessels will be described with reference to FIG.

The chemical analyzer of the present embodiment measures the sample automatic pipetting mechanism 304, the reagent automatic pipetting mechanism 308, the reaction vessel 301, the piezoelectric element 103 provided at the bottom of the reaction vessel 301, and the properties of the sample. Sensor 322, a tube 324 for introducing the sample into the sensor 322 from the bottom of the container, and a pump 323 for moving the sample. The sample automatic pipetting mechanism 304 and the reagent automatic pipetting mechanism 301 are operated, and the reaction container 301
A predetermined amount of sample and reagent are discharged into the inside. The piezoelectric element 103 starts vibrating in synchronization with this ejection timing, and as a result,
The reagent and sample are mixed in a relatively short time that induces acoustic flow. The mixed sample is operated by the pump 323, moved to the sensor unit 322, guided, and measured.

The installation position and configuration of the piezoelectric element 103 may be configured as shown in FIGS. 3 to 6.

The labor of inserting a stirring means into the container,
Since the time for washing the stirring means can be saved, time-efficient analysis can be performed.

Further, by using this mixing reaction apparatus, the liquids can be mixed and reacted without coming into contact with the liquids in the tank. In particular, it is suitable for mixing a small amount of reagent, for mixing a valuable sample, or for mixing for avoiding contamination with impurities through a spatula or the like.

The above-described embodiments have been described by taking the blood analyzer as an example, but the present invention is not limited to this.

Ultrasonic waves are used to stir the object to be stirred in a non-contact manner. By adjusting the intensity and irradiation time, it is possible to prevent denaturation of proteins such as blood and thus non-invasively. However, it goes without saying that it is not necessary to consider that even if the object to be stirred is not invaded by ultrasonic waves, it will be denatured.

[0049]

As described above, according to the chemical analyzer of the present invention, carry-over can be prevented and the chemical analyzer can be downsized.

[Brief description of drawings]

FIG. 1 is a perspective view of a chemical analyzer according to an embodiment of the present invention.

FIG. 2 is a configuration diagram of a non-invasive stirring device mounted on a chemical analyzer.

FIG. 3 is a diagram showing a non-invasive stirring device according to an embodiment of the present invention.

FIG. 4 is a diagram showing a non-invasive stirring device according to another embodiment of the present invention.

FIG. 5 is a view showing a non-invasive stirring device according to another embodiment of the present invention.

FIG. 6 is a view showing a non-invasive stirring device according to another embodiment of the present invention.

FIG. 7 is a perspective view of a chemical analyzer according to another embodiment of the present invention.

[Explanation of symbols]

101 ... Solute, 102 ... Solvent, 103 ... Piezoelectric element, 30
DESCRIPTION OF SYMBOLS 1 ... Reaction container, 203 ... Acoustic rectilinear flow, 313 ... Controller, 601, ... Acoustic lens, 304 ... Sample automatic pipetting mechanism, 308 ... Automatic reagent pipetting mechanism, 312, 316 ... Non-invasive stirring device, 314 ... Measuring part, 315 ... Cleaning mechanism, 318 ... Control section.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Hideo Enoki 502 Jinritsucho, Tsuchiura-shi, Ibaraki Machinery Research Institute, Hiritsu Seisakusho Co., Ltd. Factory Measuring Instruments Division

Claims (18)

[Claims] 1. A reaction container, a sample supply means for supplying a sample from an upper opening of the reaction container, a reagent supply means for supplying a reagent from an upper opening of the reaction container, and during or after the reaction. A chemical analyzer comprising: a measuring unit that measures the physical properties of the sample; and a sound analyzing unit that is provided outside the reaction container and that generates a sound wave toward the reaction container. 2. The chemical analyzer according to claim 1, wherein the sound wave generated by the sound wave generating means is an acoustic rectilinear flow. 3. The chemical analyzer according to claim 2, wherein the vibration speed of the sound wave is 0.1 mm / s or more. 4. The chemical analyzer according to claim 1, which has a mechanism for determining the position of the sound wave generating means. 5. The chemical analyzer according to claim 1, wherein the sound wave generating means is provided below the reaction container at a distance from the reaction container and generates a sound wave toward the bottom surface of the reaction container. 6. The chemical analyzer according to claim 5, wherein the sound wave is generated by the sound wave generating means in a direction deviated from the central portion of the bottom surface of the reaction vessel. 7. The chemical analysis device according to claim 5, wherein the sound wave is generated by the sound wave generating means so that the energy intensity of the sound wave converges near the central portion of the bottom surface of the reaction vessel. 8. The chemical analyzer according to claim 7, wherein the energy intensity of the sound wave is converged by an acoustic lens. 9. The chemical analyzer according to claim 1, wherein the sound wave generating means generates a sound wave toward a side surface of the reaction container. 10. A turntable in which a plurality of reaction vessels are arranged on the circumference, a turntable for samples in which a plurality of sample vessels containing samples are arranged on the circumference, and a plurality of reagents containing reagents A reagent turntable in which containers are arranged on the circumference, sample supply means for supplying a sample in the sample container from an upper opening of the reaction container, and a reagent in the reagent container to an upper opening of the reaction container In a chemical analyzer equipped with a reagent supplying means supplied from a section and a measuring means for measuring the physical properties of the sample during or after the reaction, a sound wave is provided outside the reaction vessel and a sound wave is directed toward the reaction vessel. A chemical analyzer equipped with a means for generating sound waves. 11. The chemical analyzer according to claim 10, wherein the sound wave generated by the sound wave generating means is an acoustic rectilinear flow. 12. The chemical analysis device according to claim 11, wherein the vibration speed of the sound wave is 0.1 mm / s or more. 13. The chemical analysis device according to claim 10, having a mechanism for determining the position of the sound wave generating means. 14. The sound wave generator according to claim 10, wherein the sound wave generator is provided below the reaction container at a distance from the reaction container.
A chemical analyzer for generating sound waves toward the bottom surface of the reaction vessel. 15. The chemical analyzer according to claim 14, wherein the sound wave is generated by the sound wave generating means in a direction deviated from the center of the bottom surface of the reaction vessel. 16. The chemical analysis device according to claim 14, wherein the sound wave is generated by the sound wave generating means so that the energy intensity of the sound wave converges near the central portion of the bottom surface of the reaction vessel. 17. The chemical analyzer according to claim 16, wherein the energy intensity of the sound wave is converged by an acoustic lens. 18. The chemical analyzer according to claim 10, wherein the sound wave generating means generates a sound wave toward a side surface of the reaction container.