JPH0767544A - Feed composition and feed - Google Patents

Abstract

PURPOSE:To obtain a feed composition having effect on noninfectious and infectious diarrhea of domestic animals, 'domestic fowls and pets and to prepare a feed mixed with the feed composition CONSTITUTION:This feed composition for preventing and treating noninfectious and infectious diarrhea contains one or more compounds selected from tannins consisting of (+)-catechin, (+)-gallocatechin, (+)-catechin gallate, (-)- epicatechin, (-)-epicatechin gallate, (-)-epigallocatechin, (-)-epigallocatechin gallate, free type theaflavin, theaflavin monogallate A, theaflavin monogallate B and theaflavin digallate to give a feed mixed with the feed composition. A feed composition for preventing and treating diarrhea comprises the tannins and a specific antibody against an infectious microorganism or against a toxin produced by the infectious microorganism.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention prevents non-infectious diseases caused by sudden changes in the environment and feed of livestock, poultry and pets, inadequate feeding, and infectious diarrhea induced by pathogenic bacteria such as viruses or bacteria. The present invention relates to a feed composition to be treated and a feed containing the composition. More specifically, (+)-catechin, (+)-gallocatechin, (+)-gallocatechin gallate, (-)-epicatechin, (-)-epicatechin gallate, (-)-epigallocatechin, (-) Non-infection of livestock, poultry and pet animals containing one or more compounds selected from tannins consisting of epigallocatechin gallate, free theaflavin, theaflavin monogallate A, theaflavin monogallate B and theaflavin digallate For preventing and treating sexual diarrhea, a feed containing the same and a feed containing the composition, or a virus that induces diarrhea in the tannins described above, or a specific antibody against a pathogen such as a bacterium or a specific antibody against a toxin produced by these. The present invention relates to a feed composition for preventing and treating diarrhea, which is used in combination, and a feed containing the same.

[0002]

2. Description of the Related Art The occurrence of diarrhea of livestock and poultry has become a serious problem in livestock management. For example, when it comes to cattle, digestive diseases such as diarrhea that occur in cows during lactation and rearing have a great impact on not only death but also physical exhaustion and the growth of healthy internal organs thereafter. It has become an important issue for giving (Kazunori Hashimoto, Beef Journal, Vol. 5, No. 11, 38 (1988)). Actually, the incidence of digestive diseases with diarrhea as the main symptom during lactation in beef cattle breeding is 13.3% (Uncle Nakane, Research on Livestock, Vol.33, No.1,37 ( 19
79)). In addition, the mortality rate of dairy cows during lactation and rearing is 1
The main cause of this is diarrhea and pneumonia (Hiroyuki Tojo et al., Livestock Research, Vol 40, No. 1).
1, 51, (1986)). Furthermore, regarding adult cattle,
When diarrhea occurs, a decrease in milk yield, milk quality, and meat quality is observed due to a decrease in feed efficiency even if death does not occur.
The loss of profit will be enormous.

The same can be said for pigs or pets (pets). Generally, the mother and the baby are separated and raised immediately after birth, which is a general breeding form. Since the internal bacterial flora is not formed, colonization of pathogenic bacteria is facilitated, and resistance to various stresses is weak, and these interact with each other, resulting in diarrhea, and the mortality rate is extremely high. It has a great influence on growth and causes poor growth.

The same is true for chickens, and diarrhea chicks generally have poor growth, resulting in weight loss and increase in body weight fluctuation, which adversely affects productivity, and the commercialization rate in broilers and the egg production rate in laying hens. This will cause a decline. Furthermore, it is known that the antibody-producing ability after vaccination is also reduced.

By the way, these diarrhea-causing factors are very complicated and are caused by Clostridium perfringens A.
Type, Clostridium perfringens C type, Salmonella typhimurium, Salmonella tabulin,
Campylobacter Jewney, Campylobacter
Collagen, Escherichia coli O-88, Escherichia coli O-99, Escherichia coli 987P, Staphylococcus aureus, Rotavirus KK-3, Rotavirus NCDV and other pathogenic bacteria, toxins produced by diarrhea virus, or intestinal bacterial flora caused by infection Not only infectious diarrhea caused by changes,
Many non-infectious diarrhea such as stress-induced diarrhea caused by stress applied to livestock, poultry and pets, and unexplained diarrhea in which no infectious microorganism is involved have occurred.

It is known that the causes of stress in these livestock, poultry and pets are three factors: environmental changes, nutritional changes and psychological changes. For example, overcrowding,
There are various causes of stress such as lack of exercise, lack of labor for hygiene management due to lack of labor, change in feed composition due to delivery, and long-distance transportation during weaning.

[0007] Furthermore, it is natural that postnatal primates are allowed to grow under the protection of their mothers for a certain period of time. However, for the purpose of improving productivity or economic efficiency in livestock management, it has been a general feeding method to separate and raise a mother and a baby immediately after birth, except for beef cattle. Most of the beasts separated from the mother are collected around a week after birth in a nursing facility and are raised collectively. At this time, the primordial animals just after birth are extremely low in antigenicity, and because the intestinal bacterial flora is not formed, colonization of pathogens is facilitated and resistance to various stresses is weak, and these interact with each other. It is considered that diarrhea easily occurs due to conflict.

Conventionally, in order to prevent or treat infectious diarrhea, it has been effective to administer an antibiotic to livestock, poultry and pet animals. However, according to the feed safety law enforced in January 1977, antibiotics were used. The types and amounts of substances have been regulated, and the effectiveness of antibiotics has been questioned due to the emergence and safety of antibiotic-resistant bacteria. As an alternative method, various natural antibacterial substances have come to be used, and it has been disclosed that tea extracts have anti-infective microbial activity or viral activity (JP-A-1-265023, JP-A-2-65023). 276562). However, when the tea extract is used alone for infectious diarrhea or when a feed containing the tea extract is used, the effect is weak, and it is doubtful in practical use. Further, when the specific antibody is used alone, in reality, since there are various types of pathogenic microorganisms, due to the specificity of these, diarrhea preventive effect cannot be expected, only therapeutic effect, Not practically effective.

[0009] On the other hand, regarding prevention and treatment of non-infectious diarrhea, when the primal animal is fed with sufficient amount of colostrum after birth or when stress occurs in the animal, the infectious microorganism is eventually infected. Since the intestinal microbiota changes such as in an easy environment, methods similar to those for infectious diarrhea or various probiotic agents and oligosaccharides are used, but these methods are only coping therapy. In addition, there is a problem in the sustainability of the effects of the probiotic agent and oligosaccharide. In addition, as a method of preventing stress, in order to eliminate the feeling of boredom in pigs, you can hang iron chains and old tires inside the piglet so that the pig can bite freely, or you can put the straw in the piglet every day. There is a method of circling boredom by biting a pig, but this is a passive preventive method, and the types of livestock, poultry and pet animals are also limited. Moreover, Japanese Patent Publication No. 3-17469 discloses a composition for accommodating livestock for reducing stress, but it is not expected to suppress non-infectious diarrhea. Furthermore, the method disclosed in Japanese Examined Patent Publication No. 3-70458 is the same, and this method is limited to the breeding period and breeding environment. Under such circumstances, the present situation is that no effective feed composition for suppressing the occurrence of non-infectious diarrhea in livestock, poultry and pet animals or a feed containing the same has been found yet.

[0010]

DISCLOSURE OF THE INVENTION The present invention is to provide a feed composition effective for non-infectious diarrhea and infectious diarrhea of livestock, poultry and pets, and a feed containing the same. .

[0011]

Therefore, the present inventors have
As a result of diligent research on a feed composition effective for non-infectious diarrhea of livestock, poultry and pets and a feed containing the composition, (+)-catechin, (+)-gallocatechin, (+)-gallo Catechin gallate, (−)-epicatechin, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epigallocatechin gallate, free theaflavin, theaflavin monogallate A, theaflavin monogallate B, theaflavin The inventors have found that tannins such as digallate are effective against non-infectious diarrhea, and completed the present invention. Furthermore, in addition to these tannins, when in combination with a specific antibody against a pathogenic bacterium or virus against diarrhea or a specific antibody against the toxin produced by these tannins, infectious diarrhea compared to the case of using tannins alone, They have found that they exhibit excellent effects and have completed the present invention.

The non-infectious diarrhea of the present invention refers to stress diarrhea caused by stress applied to livestock, poultry and pet animals, or diarrhea of unknown cause not involving infectious microorganisms. Infectious diarrhea refers to diarrhea caused by diarrhea causing pathogenic bacteria or viruses. The tannins used in the present invention are (+)-
Catechin, (+)-gallocatechin, (+)-gallocatechin gallate, (-)-epicatechin, (-)-epicatechin gallate, (-)-epigallocatechin, (-)-epigallocatechin gallate, free form Theaflavin, theaflavin monogallate A, theaflavin monogallate B,
Refers to polyphenol compounds such as theaflavin digallate.

The tannins used in the present invention are green tea,
The components of hot water extract of tea such as black tea and oolong tea are preferable, but they can also be obtained by the ethyl acetate-soluble fraction of water or alcohol extract of tea or ultrafiltration membrane and reverse osmosis membrane treatment. Or a large amount of extraction residue is discarded as it is during the production of beverages such as green tea, oolong tea, and black tea,
Alternatively, it may be used as a feed composition after being subjected to treatment such as crushing. Further, it may be a material derived from other raw materials such as persimmon shibu or a chemically synthesized product. And
When the obtained tannins are used in the present invention, they can be used alone, as a mixture of two or more kinds, or even a crude extract containing tannins. Such tannins are contained in large amounts as tea ingredients,
Since it is already effective in preventing caries, hyperlipidemia, cancer, etc., it is used in many foods for the prevention of diseases related thereto, and thus this safety is very high.

In order to obtain the effect of suppressing non-infectious diarrhea in the present invention, it is necessary to use livestock, poultry and pet animals,
The tannins are usually 0.5 to 50 mg / kg body weight, more preferably 2 to 20 mg / kg body weight per day. If the dose is less than 0.5 mg / kg body weight, the effect of suppressing non-infectious diarrhea cannot be expected and
If the amount exceeds g / kg of body weight, the tannins have an antibacterial action, which affects the gastrointestinal microorganisms that are resident in livestock, poultry and pets. On the other hand, in order to expect the effect of suppressing infectious diarrhea, the dose when used in combination with a specific antibody is usually 1 tannin.
The daily dose is 0.3 to 25 mg / kg body weight, more preferably 2 to 15 mg / kg body weight. Dosage 0.3mg
If the weight is lower than kg, the effect of suppressing infectious diarrhea cannot be expected. The tannins can be represented by the amount of tannin quantified by the official tannin analysis method.

The specific antibody of the present invention refers to an antibody that specifically binds to infectious microorganisms of livestock, poultry and pets or toxins produced by these, and the types of antibodies include livestock, poultry and poultry. It refers to chicken egg antibodies obtained from eggs of laying hens hyperimmunized with infectious microorganisms of companion animals and milk antibodies obtained from milk of mammals, and the antibody purity is not limited. That is, the antibody may be a pure product, and the chicken egg antibody is not particularly limited, but the whole egg, egg yolk, whole egg liquid, yolk liquid, whole egg powder, egg yolk powder or water-soluble protein of egg yolk containing this is not particularly limited. It may be a fraction powder. Also,
The milk antibody is not particularly limited, but may be whole milk powder, skim milk powder containing this, or whey protein powder.

The infectious microorganisms of livestock, poultry and pet animals are not particularly limited, but include, for example, Clostridium perfringens type A and Clostridium.
Perfringens type C, Salmonella typhimurium, Salmonella tabulin, Campylobacter jejuni, Campylobacter collie, E. coli O-8
8, Escherichia coli O-99, Escherichia coli 987P, Staphylococcus aureus, Rotavirus KK-3, Rotavirus NCDV and other pathogenic bacteria, and diarrhea virus. As an animal hyperimmunized with an infectious disease microorganism such as these or a toxin produced by these, any animal can be used as long as it can produce a specific antibody against the microorganism, but infection of livestock, poultry and pet animals which is the object of the present invention. From the viewpoint of practical use of antibodies against sex microorganisms and application of this antibody composition, laying hens or mammals such as cows, goats, and sheep that can produce large amounts of specific antibodies are particularly desirable. Among these, from the viewpoints of workability of hyperimmunity, antibody production ability, animal breeding cost, etc., a method of immunizing a laying hen with a microorganism or a toxin produced by the hen and obtaining an antibody from the hen's egg is most preferable.

As a method for hyperimmunizing a laying chicken, livestock,
It is advisable to increase specific antibody in chicken eggs by repeatedly immunizing laying hens with the infectious microorganisms of poultry and pet animals or toxins produced by them as an antigen. As a method of hyperimmunizing mammals, it is advisable to repeatedly immunize animals such as cows, goats and sheep with the antigen to increase the specific antibody in milk.

The antigen used in this case may be prepared by a known method. For example, a large amount of infectious microorganisms of livestock, poultry and pet animals may be cultivated, then attenuated or inactivated by a known method and used as an antigen.

As a method for immunizing laying hens or mammals with the antigen, any method such as intramuscular injection, subcutaneous injection, intravenous injection, intraperitoneal injection or oral immunization with drinking water may be used.

The immunization with the antigen is repeated until the antibody titer reaches its maximum value while examining the specific antibody titer appearing in chicken eggs or milk by a method such as enzyme immunoassay.
The antibody titer can be maintained at a certain level or more of specific antibody throughout the spawning period or the colostrum period by repeatedly immunizing the laying hen or mammal with the antigen at appropriate intervals.

The amount of the antigen to be immunized varies depending on the type of animal to be immunized, the type of antigen, etc., and therefore it is necessary to select it by a timely preliminary test, etc. In the case of viruses, the virus load is 10
An antigen dose of μg to 1 mg / bird / dose is selected.

After immunizing a laying hen or a mammal with an infectious disease microorganism of livestock, poultry and pets or a toxin produced by the same as an antigen, chicken eggs or milk containing a specific antibody against the antigen are collected, from which the present invention is prepared. Specific antibodies to infectious microorganisms of livestock, poultry and pets used for the products or toxins produced by these can be prepared.

When using chicken eggs, after breaking eggs, the whole egg liquid or yolk liquid is separated and homogenized with a homogenizer or the like,
It is possible to obtain whole egg powder or egg yolk powder containing a specific antibody against the antigen that has been sterilized and dried by hot air or freeze-drying. Further, a known chicken egg antibody purification method from the egg yolk liquid or egg yolk powder (JP-A-64-38098, Agric. Bio).
l. Chem. , Vol. 54, No. 10,2531
-2535 (1990), etc., an egg yolk water-soluble protein powder or a specific antibody pure product powder having an increased purity of the specific antibody against the used antigen can be prepared.

When milk is used, whole milk powder containing a specific antibody against an antigen used by sterilizing the milk or skim milk obtained by separating lipid components in the milk with a cream separator or the like and then drying with hot air or freeze-drying Milk or skimmed milk powder is obtained. From the milk or the skimmed milk, a whey protein powder or a pure product of the specific antibody having an increased purity of the specific antibody against the used antigen can be prepared by a known method. When the present invention-specific chicken egg antibody is used, in order to expect an inhibitory effect on infectious diarrhea, the dose is 1 mg which has an antibody titer of 1.5 times or more that of the blank by enzyme immunoassay. / Body weight kg or more, more preferably 5 mg / body weight kg. If the dose is less than 1 mg / kg body weight, the expected effect cannot be obtained, which is not preferable.

In the present invention, livestock and poultry mean cows,
Pig, horse, goat, sheep, chicken, turkey, duck,
It refers to animals that are bred for industrial purposes such as quail, and pet animals are animals that are bred for personal hobbies such as dogs and cats. Further, the product of the present invention can be used for all livestock, poultry and pet animals, but from the viewpoint of effect or palatability, herbivorous animals can be easily ingested regardless of the bitterness of tannins, so that Preferable are cows such as dairy cows and beef, and goats, sheep, and deer, which are particularly industrially useful. In addition, the product of the present invention may be administered as a composition alone to livestock, poultry and pet animals, or may be directly added to feed, and the form of use is not particularly limited. And when adding to feed, this addition method, addition timing, etc. are not particularly limited.

Hereinafter, the present invention will be described in detail with reference to examples, but the present invention is not limited to the examples.

[0027]

【Example】

Example 1 1 kg of green tea was mixed with about 15 liters of water and stirred at 80 ° C.
It was extracted for 3 hours. The extract obtained by filtration was spray-dried to obtain 350 g of a hot water extract of green tea containing tannins having a purity of 25%. The composition of the obtained tannins is
(+)-Catechin 1.2%, (+)-gallocatechin 5.
0%, (+)-gallocatechin gallate 3.9%, (-)
-Epicatechin 2.3%, (-)-epicatechin gallate 1.5%, (-)-epigallocatechin gallate 5.0% and (-)-epigallocatechin gallate 6.1%.

Example 2 200 g of green tea was extracted with 4 liters of hot water at 85 ° C. with stirring for 30 minutes, and tea leaves were removed by filtration to obtain 2.5 liters of an extract. This liquid was passed through an ultrafiltration device (manufactured by DDS, membrane type GR-81PP, molecular weight cutoff 6000) to obtain 2 liters of a passing liquid. To the concentrated residual liquid, 1 liter of water was added and the same operation was performed to obtain 1.2 liters of the passing liquid. Both solutions were combined and concentrated with a reverse osmosis membrane (membrane type HC-50, manufactured by DDS) to 200 ml, and then freeze-dried,
Hot water extract of tea containing tannins with a purity of 29% 48.6
g was obtained. The composition of the obtained tannins is (+)-
Catechin 1.4%, (+)-gallocatechin 5.8%,
(+)-Gallocatechin gallate 4.5%, (-)-Epicatechin 2.7%, (-)-Epicatechin gallate 1.
8%, (-)-epigallocatechin 5.8% and (-)-
The epigallocatechin gallate was 7.0%.

Example 3 To 350 g of the hot water extract obtained in Example 1, 8 liters of water was added and dissolved, and then partitioned with hexane and chloroform successively. Ethyl acetate (10 liters) was added to the aqueous layer after partitioning, and the mixture was vigorously stirred and allowed to stand, then the ethyl acetate layer was separated, and the ethyl acetate was distilled off and dried to obtain 70 g of an ethyl acetate-soluble fraction (tannins). 74.5% as a mixture of). The content of total tannins in the ethyl acetate-soluble fraction was 74.5%, the proportion of each tannin was (+)-catechin 3.5%,
(+)-Gallocatechin 14.8%, (+)-Gallocatechin gallate 11.6%, (-)-Epicatechin 7%,
The amount of (-)-epicatechin gallate was 4.6%, the amount of (-)-epigallocatechin was 15.0%, and the amount of (-)-epigallocatechin gallate was 18.0%.

Example 4 Salmonella tabulin, Escherichia coli O-88, Escherichia coli O-99 and Escherichia coli 987P, which are known to be the causative bacteria of infectious diarrhea in cattle, were cultured in a brain heart infusion medium. In addition, bovine rotavirus KK-
3, bovine rotavirus NCDV each Eagle MEM
Rhesus monkey-derived MA104 cells cultured in a medium were used as a host. Laying hens were hyperimmunized with the respective bacteria or viruses as the antigen. From the egg yolk 10 kg produced by this laying hen, 45 g of a specific egg antibody specific to the fungus
Respectively obtained.

Test Example 1 30 calves were divided into 3 groups with 10 calves each, the group fed only artificial milk was group A, and the artificial milk and the tannins prepared in Example 1 were 60 mg per day. The group to which the added artificial milk was added was Group B, and the group to which the artificial milk and the artificial milk to which 100 mg of the tannins prepared in Example 1 were added were given as C.
The group was raised for 5 weeks. In addition, artificial milk and water were freely taken. Then, the fecal score and the intestinal microbiota were measured from 0 to 5 weeks after the start of the test as an index showing non-infectious diarrhea. The fecal score is a numerical value of the fecal properties. The results are shown in Table 1 and Table 2, respectively.

[0032]

[Table 1]

[0033]

[Table 2]

As is clear from Tables 1 and 2, calves bred with artificial milk containing 100 mg of tannins of group C had a reduced fecal score and decreased C. perfringens and Escherichia coli, and Bifidobacterium. The increase in lactic acid bacteria effectively suppressed non-infectious diarrhea as compared with calves in groups A and B. The same test was conducted using the tannins obtained in Examples 2 and 3, but the same result was obtained. Further, the same type of test was conducted using tannins derived from black tea and choiryu tea, but similar results were obtained.

Test Example 2 15 adult cattle immediately after calving were divided into 3 groups of 5 cows each, the group fed the basic feed shown in Table 3 was group D, and 1 kg of the basic feed shown in Table 3 was used in Example 1. The group fed with the feed containing 10 g of the tannins prepared in 1. was designated as group E, and the group fed with the feed containing 15 g of the tannins prepared in Example 1 added to the basic diet shown in Table 5 was designated as group F for 5 weeks. did. The feed and water were taken freely. Then, as an index showing non-infectious diarrhea, in the same manner as in Test Example 1, the fecal score and the intestinal microbiota were measured from 0 to 5 weeks after the start of the test. The results are shown in Table 4 and Table 5, respectively.

[0036]

[Table 3]

[0037]

[Table 4]

[0038]

[Table 5]

As can be seen from Tables 4 and 5, the adult cows of group F in which 15 g of tannins were added to 1 kg of the basic feed shown in table 3 were compared with adult cows raised in groups D and E. , Fecal score decreased, C. perfringens and coliforms decreased, bifidobacteria and lactic acid bacteria increased, and stress-induced (non-infectious) diarrhea caused by delivery was effectively suppressed. The same test was conducted using the tannins obtained in Examples 2 and 3, but the same result was obtained. Furthermore, the same type of test was conducted using tannins derived from black tea and Toryu tea, and similar results were obtained.

Test Example 3 10 calves infected with Salmonella tabulin were treated with 10 calves.
The heads were divided into 3 groups, the group to which only artificial milk was given was a group G (no addition group), and the group to which artificial milk to which the tannins prepared in Example 1 were added at 30 mg per day was given to the artificial milk group. Example 1 was added to H group (group containing only tannins) and artificial milk.
The group to which 30 mg of the tannins prepared in 1 and the specific egg egg antibody prepared in Example 4 was added so as to be 400 mg per day was designated as a group I (tannins and specific egg egg antibody added group) 5 Raised for a week. Then, the fecal score and the number of salmonella tabulin in the feces as indexes showing diarrhea were determined from 0 to 5 weeks after the start of the test. The results are shown in Table 6 and Table 7, respectively.

[0041]

[Table 6]

[0042]

[Table 7]

From Tables 6 and 7, the fecal score of group I was lower than that of the calves of group G and group H, and the number of salmonella tabulin in feces was decreased, so that infectious diarrhea was effective. It turned out that it was suppressed. The same test was conducted using the tannins obtained in Examples 2 and 3, but the same result was obtained. Furthermore, the same test was conducted using tannins derived from black tea and choiryu tea, but similar effects were obtained. In addition, E. coli 0-88, E. coli 0-
99, Escherichia coli 987P, bovine rotavirus KK-3 and bovine rotavirus NCDV were tested for similar infectious diarrhea, but similar results were obtained.

Test Example 4 15 adult cattle infected with Salmonella tabulin were divided into 3 groups of 5 cows each, and the group fed with the basic diet shown in Table 3 was designated as J.
Group (non-addition group), group in which 7.5 g of tannins prepared in Example 1 was added to 1 kg of basic feed, Group K (group in which only tannins were added), tannins prepared in Example 1 in 1 kg of basic feed A group to which 7.5 g and 6 g of the specific egg egg antibody prepared in Example 4 were added was fed as a group L (tannins and specific egg egg antibody added group) for 5 weeks. The feed and water were taken freely. Then, as in Test Example 3, the stool score and the number of salmonella tabulin in the stool were determined from 0 to 5 weeks after the start of the test as an index showing diarrhea. The results are shown in Table 8 and Table 9, respectively.

[0045]

[Table 8]

[0046]

[Table 9]

From Tables 8 and 9, the adult cows of group L are J group and K group.
Infectious diarrhea was effectively suppressed compared with the adult cows in the herd.
The same test was conducted using the tannins obtained in Examples 2 and 3, but the same result was obtained. Further, the same type of test was conducted using tannins derived from black tea and choiryu tea, and similar results were obtained. Also E. coli 0
Similar tests were performed using -88, E. coli 0-99, E. coli 987P, bovine rotavirus KK-3 and bovine rotavirus NCDV, but similar results were obtained.

[0048]

INDUSTRIAL APPLICABILITY As described above, the product of the present invention has a great effect on non-infectious diarrhea and infectious diarrhea of livestock, poultry and pet animals, and the tannins contained in the product of the present invention Also has a deodorizing effect, it also has a deodorizing effect on feces and urine excreted by livestock, poultry and pets, and it greatly contributes to the livestock industry and pet industry.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Shigemitsu Akachi 9-5 Akahori Shinmachi, Yokkaichi-shi, Mie Taiyo Kagaku Co., Ltd.

Claims (3)

[Claims] 1. (+)-Catechin, (+)-gallocatechin, (+)-gallocatechin gallate, (-)-epicatechin, (-)-epicatechin gallate, (-)-epigallocatechin, (-) ) -Epigallocatechin gallate, free theaflavin, theaflavin monogallate A, theaflavin monogallate B, non-infectious characterized by containing one or more compounds selected from tannins consisting of theaflavin digallate A feed composition for preventing and treating diarrhea and a feed containing the same. 2. (+)-Catechin, (+)-gallocatechin, (+)-gallocatechin gallate, (-)-epicatechin, (-)-epicatechin gallate, (-)-epigallocatechin, (-) ) -Epigallocatechin gallate, free theaflavin, theaflavin monogallate A, theaflavin monogallate B, theaflavin digallate, or one or more compounds selected from diarrhea characterized by using a specific antibody in combination A preventive and therapeutic feed composition and a feed containing the same. 3. The feed composition according to claim 1, wherein the feed composition and the feed containing the feed composition are for herbivores, and the feed containing the feed composition.