JPH0767487B2 - Living body transplant - Google Patents
Living body transplantInfo
- Publication number
- JPH0767487B2 JPH0767487B2 JP63006564A JP656488A JPH0767487B2 JP H0767487 B2 JPH0767487 B2 JP H0767487B2 JP 63006564 A JP63006564 A JP 63006564A JP 656488 A JP656488 A JP 656488A JP H0767487 B2 JPH0767487 B2 JP H0767487B2
- Authority
- JP
- Japan
- Prior art keywords
- chitin
- calcium phosphate
- phosphate compound
- bone
- hydroxyapatite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 229920002101 Chitin Polymers 0.000 claims description 27
- 239000000463 material Substances 0.000 claims description 11
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical class [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 8
- 210000000988 bone and bone Anatomy 0.000 description 22
- 239000001506 calcium phosphate Substances 0.000 description 21
- 229910000389 calcium phosphate Inorganic materials 0.000 description 17
- -1 calcium phosphate compound Chemical class 0.000 description 17
- 235000011010 calcium phosphates Nutrition 0.000 description 17
- 230000007547 defect Effects 0.000 description 11
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 11
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 11
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 8
- 230000003239 periodontal effect Effects 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 239000008187 granular material Substances 0.000 description 7
- 239000007943 implant Substances 0.000 description 7
- 210000002808 connective tissue Anatomy 0.000 description 6
- 230000006196 deacetylation Effects 0.000 description 6
- 238000003381 deacetylation reaction Methods 0.000 description 6
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 6
- 239000004372 Polyvinyl alcohol Substances 0.000 description 5
- 229920002451 polyvinyl alcohol Polymers 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 241000282472 Canis lupus familiaris Species 0.000 description 4
- 239000004744 fabric Substances 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 229910000391 tricalcium phosphate Inorganic materials 0.000 description 4
- 235000019731 tricalcium phosphate Nutrition 0.000 description 4
- 229940078499 tricalcium phosphate Drugs 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 229920001661 Chitosan Polymers 0.000 description 3
- FXHOOIRPVKKKFG-UHFFFAOYSA-N N,N-Dimethylacetamide Chemical compound CN(C)C(C)=O FXHOOIRPVKKKFG-UHFFFAOYSA-N 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 239000004745 nonwoven fabric Substances 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 230000015271 coagulation Effects 0.000 description 2
- 238000005345 coagulation Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 201000001245 periodontitis Diseases 0.000 description 2
- 235000011121 sodium hydroxide Nutrition 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 229910014497 Ca10(PO4)6(OH)2 Inorganic materials 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- FHKPLLOSJHHKNU-INIZCTEOSA-N [(3S)-3-[8-(1-ethyl-5-methylpyrazol-4-yl)-9-methylpurin-6-yl]oxypyrrolidin-1-yl]-(oxan-4-yl)methanone Chemical compound C(C)N1N=CC(=C1C)C=1N(C2=NC=NC(=C2N=1)O[C@@H]1CN(CC1)C(=O)C1CCOCC1)C FHKPLLOSJHHKNU-INIZCTEOSA-N 0.000 description 1
- JAWMENYCRQKKJY-UHFFFAOYSA-N [3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-ylmethyl)-1-oxa-2,8-diazaspiro[4.5]dec-2-en-8-yl]-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]methanone Chemical compound N1N=NC=2CN(CCC=21)CC1=NOC2(C1)CCN(CC2)C(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F JAWMENYCRQKKJY-UHFFFAOYSA-N 0.000 description 1
- 229910052586 apatite Inorganic materials 0.000 description 1
- 210000004763 bicuspid Anatomy 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000000035 biogenic effect Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000007872 degassing Methods 0.000 description 1
- 230000000994 depressogenic effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 description 1
- 235000011007 phosphoric acid Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000002759 woven fabric Substances 0.000 description 1
Landscapes
- Materials For Medical Uses (AREA)
- Dental Preparations (AREA)
Description
【発明の詳細な説明】 （産業上の利用分野） 本発明は，生体用移植材に関するものであり，さらに詳
しくは歯周骨欠損等，骨，歯等の硬組織に使用する生体
用移植材に関するものである。Description: TECHNICAL FIELD The present invention relates to a biomedical implant, and more specifically, to a biogenic implant used for hard tissues such as periodontal bone defects and bones and teeth. It is about.
（従来の技術） 近年，辺縁性歯周炎の病因がかなり明確となり，歯周炎
の進行を抑えることがそれほど困難でなくなり，破壊さ
れた歯周支持組織を回復させるために骨移植材の開発が
進んでいる。(Prior Art) In recent years, the etiology of marginal periodontitis has become quite clear, and it is not so difficult to suppress the progression of periodontitis, and bone grafting materials have been used to restore the destroyed periodontal supporting tissue. Development is in progress.
その中でも，ハイドロキシアパタイトやトリカルシウム
ホスフエート等のリン酸カルシウム化合物が現在，広く
使用されており，これらに関して多くの動物実験及び臨
床試験が行われているが，特に，歯周骨欠損移植材とし
て比較的良好な成績が得られている。これらの移植材
は，リン酸カルシウム化合物が顆粒状，ブロック状等の
形で歯周の骨欠損部に充填埋没され，経日の後，結合組
織及び新生骨の生成が行われるものである。Among them, calcium phosphate compounds such as hydroxyapatite and tricalcium phosphate are widely used at present, and many animal experiments and clinical trials have been carried out on these compounds. Good results have been obtained. In these transplant materials, calcium phosphate compounds are filled and embedded in the periodontal bone defect portion in the form of granules, blocks, etc., and connective tissue and new bone are generated after a lapse of time.
（発明が解決しようとする課題） しかしながら，従来の顆粒状やブロック状のリン酸カル
シウム化合物の移植材では，移植材を充填した後に骨欠
損部が変形したり，移植材が欠損部からはみ出したりす
ることが起こる場合等があり，また，移植材周辺での新
生骨や結合組織の生成も不十分で，良好な歯周支持組織
を完成することが難しかった。(Problems to be Solved by the Invention) However, in the conventional granular or block-shaped calcium phosphate compound implants, the bone defect may be deformed after the implant is filled, or the implant may protrude from the defect. However, it was difficult to complete a good periodontal support tissue because the formation of new bone and connective tissue around the implant was insufficient.
本発明は，骨欠損部が変形したり，欠損部からはみ出し
たりすることがなく，また，周辺での新生骨や結合組織
の生成も十分な移植材を提供することを目的とするもの
である。An object of the present invention is to provide a transplant material in which a bone defect portion is not deformed or protrudes from the defect portion, and the generation of new bone or connective tissue in the periphery is sufficient. .
（課題を解決するための手段） 本発明者は，上記の課題を解決するため鋭意研究を重ね
た結果，移植材であるリン酸カルシウム化合物を歯，骨
等の硬組織の欠損部へ充填する際に，予めリン酸カルシ
ウム化合物をキチンで固定するかあるいは包囲しておく
ことにより，上記の目的が達成し得ることを見出し，本
発明に到達した。(Means for Solving the Problems) As a result of intensive studies to solve the above problems, the present inventor has found that when a calcium phosphate compound, which is a transplant material, is filled in a defect portion of hard tissue such as teeth and bones. The inventors have found that the above object can be achieved by fixing or surrounding a calcium phosphate compound with chitin in advance, and arrived at the present invention.
すなわち本発明は，粉末状，顆粒状あるいはブロック状
のリン酸カルシウム化合物がキチンで固定されるか又は
キチンで包囲されたことを特徴とする生体用移植材を要
旨とするものである。That is, the gist of the present invention is a bioimplant which is characterized in that powdery, granular or block-like calcium phosphate compounds are fixed with chitin or surrounded by chitin.
キチンは，甲殻類，昆虫類等の外骨格を塩酸処理並びに
カ性ソーダ処理して灰分及び蛋白質を除去した残分で，
アミノ多糖類の一種であるが，本発明にいうキチンに
は，キチンそのものの他に，その誘導体も含まれる。好
ましいキチンの誘導体としては，例えば，脱アセチル化
度が数％〜100％に至る各種の脱アセチル化物（脱アセ
チル化度100％あるいはそれに近い脱アセチル化度のも
のは一般にキトサンと呼称されている。），キチンのエ
ーテル化物，エステル化物，カルボキシメチル化物，ヒ
ドロキシエチル化物,o−エチル化物等があげられる。Chitin is a residue obtained by treating the exoskeleton of crustaceans, insects, etc. with hydrochloric acid and caustic soda to remove ash and proteins.
Chitin, which is a kind of aminopolysaccharide, includes not only chitin itself but also its derivatives. As preferable chitin derivatives, for example, various deacetylated products having a deacetylation degree of several% to 100% (a deacetylation degree of 100% or a deacetylation degree close to 100% is generally called chitosan). ), An etherified product, an esterified product, a carboxymethylated product, a hydroxyethylated product and an o-ethylated product of chitin.
本発明におけるリン酸カルシウム化合物の好ましい具体
例としては，ハイドロキシアパタイト，トリカルシウム
ホスフエートがあげられる。ハイドロキシアパタイト
は，Ca10(PO4)6(OH)2の化学式で示されるリン酸系カル
シウムアパタイトであり，例えば，Ca(OH)2懸濁液中に
正リン酸水溶液を滴下し，生成物を1000〜1200℃の温度
で熱処理して製造することができる。その際の反応温
度,pH,濃度等の条件により，得られるハイドロキシアパ
タイトの結晶形態が変化するが，本発明においてはその
いずれをも使用できる。また，トリカルシウムホスフエ
ートは上記生成物を1300℃以上で熱処理することにより
製造することができる。Preferred specific examples of the calcium phosphate compound in the present invention include hydroxyapatite and tricalcium phosphate. Hydroxyapatite is a phosphate-type calcium apatite represented by the chemical formula of Ca 10 (PO 4 ) 6 (OH) 2 , and for example, an aqueous solution of orthophosphoric acid is dripped into a Ca (OH) 2 suspension to produce the product. Can be manufactured by heat treatment at a temperature of 1000 to 1200 ° C. The crystal form of the hydroxyapatite obtained varies depending on the reaction temperature, pH, concentration and other conditions at that time, and any of them can be used in the present invention. Tricalcium phosphate can be produced by heat-treating the above product at 1300 ° C or higher.
本発明にいうキチンで固定されたリン酸カルシウム化合
物とは，例えば，粉末状，顆粒状あるいはブロック状の
リン酸カルシウム化合物をキチンで接合したり，被覆し
たりなどして成形物としたものを意味し，また，キチン
で包囲されたリン酸カルシウム化合物とは，例えば，キ
チンの不織布，織物，編物等に粉末状，顆粒状あるいは
ブロック状のリン酸カルシウム化合物を包み込んだもの
を意味する。The term "chitin-fixed calcium phosphate compound" as used in the present invention means, for example, a powdered, granular or block-shaped calcium phosphate compound bonded with chitin or coated to form a molded article. The calcium phosphate compound surrounded by chitin means, for example, a non-woven fabric, a woven fabric, or a knitted fabric of chitin in which a powdery, granular, or block calcium phosphate compound is wrapped.
キチンで固定されたリン酸カルシウム化合物を調整する
には，例えば，リン酸カルシウム化合物の顆粒を，溶媒
としてジメチルアセトアミドと塩化リチウムの混合液を
使用したキチン溶液中に分散させ，水で凝固及び水洗を
行った後に乾燥すればよい。また，キチン溶液にリン酸
カルシウム化合物とポリビニルアルコールを分散させ，
冷水で凝固した後，熱水でポリビニルアルコールを溶出
させ，さらに，凍結乾燥することによりキチンで固定さ
れた多孔質のリン酸カルシウム化合物を調整することが
できる。また，キチンで包囲されたリン酸カルシウム化
合物を調整するには，例えば，キチンの不織布や編物で
作製した筒の中にリン酸カルシウム化合物を入れて包み
込むか又は患部にキチンの不織布や編物を挿入した後，
リン酸カルシウム化合物の粉末又は顆粒を必要なだけ充
填したのち，充填したリン酸カルシウム化合物を先に挿
入したキチンの不織布や編物で包み込めばよい。To prepare a calcium phosphate compound fixed with chitin, for example, granules of a calcium phosphate compound are dispersed in a chitin solution using a mixture of dimethylacetamide and lithium chloride as a solvent, and after coagulation and washing with water, Just dry. Also, disperse the calcium phosphate compound and polyvinyl alcohol in the chitin solution,
After coagulation with cold water, polyvinyl alcohol is eluted with hot water and freeze-dried to prepare a porous calcium phosphate compound fixed with chitin. Further, in order to adjust the calcium phosphate compound surrounded by chitin, for example, after putting the calcium phosphate compound in a cylinder made of a non-woven or knitted fabric of chitin and wrapping or inserting the non-woven or knitted fabric of chitin into the affected area,
After filling powder or granules of the calcium phosphate compound as much as necessary, the filled calcium phosphate compound may be wrapped with a non-woven or knitted fabric of chitin.
このようにして調整する本発明の移植材の大きさは，と
くに限定されるものではない。例えば，患部が小さすぎ
た場合，移植材を鋏やナイフ等で充填容積に必要な大き
さに変形させて使用すればよい。The size of the implant material of the present invention adjusted in this manner is not particularly limited. For example, when the affected area is too small, the transplant material may be deformed to a size necessary for the filling volume with scissors or a knife before use.
（実施例） 以下，本発明を実施例によりさらに具体的に説明する。(Examples) Hereinafter, the present invention will be described more specifically with reference to Examples.
実施例1,比較例１ キチン粉末（新日本化学製，脱アセチル化度５％）を8w
/w％の塩化リチウムを含むジメチルアセトアミド溶液中
に2w/w％の濃度で溶解し，キチンドープを得た。このド
ープ100gに,25gのハイドロキシアパタイトのＧ−Ｍ型の
顆粒（旭光学工業製，商品名：アパセラム）及び25gの
ポリビニルアルコール（ユニチカ化成製,UF−170GS）を
加えてよく混合したのち，厚さ5mmの板状にし，次いで
冷水により十分に凝固した。その後,98℃の熱水でポリ
ビニルアルコールが流出しなくなるまで処理した。処理
物を凍結乾燥して水を除去したところ，キチンのマトリ
ックス中にハイドロキシアパタイトが分散した軟らかい
多孔質の成形物が得られた。Example 1 and Comparative Example 1 8 w of chitin powder (manufactured by Shin Nippon Chemical Co., Ltd., deacetylation degree 5%)
A chitin dope was obtained by dissolving it in a dimethylacetamide solution containing / w% lithium chloride at a concentration of 2w / w%. To 100 g of this dope, 25 g of hydroxyapatite GM type granules (manufactured by Asahi Optical Co., Ltd., trade name: Apaceram) and 25 g of polyvinyl alcohol (manufactured by Unitika Kasei, UF-170GS) were added and mixed well. It was made into a plate with a thickness of 5 mm and then solidified sufficiently with cold water. Then, it was treated with hot water at 98 ° C until polyvinyl alcohol stopped flowing out. When the treated product was freeze-dried to remove water, a soft porous molded product in which hydroxyapatite was dispersed in the chitin matrix was obtained.
一方，ビーグル犬の上顎の第２小臼歯及び第４小臼歯を
抜歯した。抜歯後,50日以上経過した後に，垂直性骨欠
損を作成した。すなわち，粘膜骨膜弁を作り，骨面より
剥離して歯根面，骨面を露出させた。第２小臼歯には，
実施例１の多孔質の成形物を充填部と同じ大きさに加工
したものを充填した。その後，粘膜骨膜弁を元に戻し，
縫合した。On the other hand, the second and fourth premolars of the upper jaw of the beagle dog were extracted. Vertical bone defects were created 50 days or more after tooth extraction. That is, a mucoperiosteal flap was made and peeled from the bone surface to expose the root surface and the bone surface. For the second premolar,
The porous molded product of Example 1 processed into the same size as the filling part was filled. Then, replace the mucoperiosteal flap,
Sutured.
比較のため，ハイドロキシアパタイトの顆粒（比較例
１）をそのまま第４小臼歯部に充填したのち，上記の場
合と同様にして，最後は粘膜骨膜弁を元に戻し，縫合し
た。For comparison, the granules of hydroxyapatite (Comparative Example 1) were directly filled in the fourth premolar portion, and then the mucoperiosteal flap was finally restored and sutured in the same manner as above.
４カ月後，犬を屠殺して観察したところ，実施例１の移
植材を使用した場合は，歯槽骨が充填部に完成して隆起
し，良好な歯周支持組織が生成しており，組織学的な観
察でも，ハイドロキシアパタイトの顆粒の間隙に結合組
織及び新生骨が認められた。これに対し，比較例１の移
植材を使用した場合は，充填場所の表面が陥没し，凹面
となっており，組織学的観察でも，結合組織及び新生骨
の生成が十分でなかった。Four months later, when the dog was slaughtered and observed, when the transplant material of Example 1 was used, the alveolar bone was completed and raised in the filling part, and good periodontal support tissue was generated. The connective tissue and new bone were also observed in the spaces between the hydroxyapatite granules. On the other hand, when the graft material of Comparative Example 1 was used, the surface of the filling place was depressed and was concave, and the formation of connective tissue and new bone was not sufficient even by histological observation.
実施例２ キチン粉末（新日本化学製，脱アセチル化度５％）を8w
t％の塩化リチウムを含むジメチルアセトアミド溶液中
に8w/w％の濃度で溶解し，キチンドープを得た。得られ
たドープを1480メツシユの金網で濾過し，放置脱泡の上
タンクに入れ，加圧下でギヤーポンプにて輸送し，口径
0.08mm,500ホールのノズルより吐出させ,80℃の熱水で
凝固した後,10m/minの紡速で引き取り，洗浄の上乾燥し
た。得られた繊維の単糸デニールは0.8dであった。これ
を5mmの長さに切断し,20％カ性ソーダ水溶液を用い,110
℃で１時間処理した。さらに，洗浄を十分に行った後，
乾燥し，これに重量比でキチン繊維９に対して１の割合
のポリビニルアルコール製バインダー（ユニチカ化成
製,SML）を混合し,3mg/cm2の日付けで抄紙を行い，不織
布を作製した。Example 2 8 w of chitin powder (manufactured by Shin Nippon Chemical Co., Ltd., deacetylation degree 5%)
It was dissolved in a dimethylacetamide solution containing t% lithium chloride at a concentration of 8 w / w% to obtain a chitin dope. The obtained dope was filtered with a wire mesh of 1480 mesh, left standing degassing, put in a tank, and transported by a gear pump under pressure.
It was discharged from a nozzle of 0.08 mm, 500 holes, coagulated with hot water at 80 ° C, taken up at a spinning speed of 10 m / min, washed and dried. The fiber had a single yarn denier of 0.8 d. This was cut to a length of 5 mm and 110% caustic soda solution was used to
It was treated at ° C for 1 hour. In addition, after thorough washing,
After being dried, a polyvinyl alcohol binder (manufactured by Unitika Kasei Co., SML) in a ratio of 1 to chitin fiber 9 by weight was mixed and papermaking was carried out at a date of 3 mg / cm 2 to prepare a nonwoven fabric.
一方，ビーグル犬の上顎の第４小臼歯を抜歯し，抜歯
後,50日以上経過した時点で，第４小臼歯に対して垂直
性骨欠損を作製した。歯根面，骨面を露出させた後，上
記の不織布を用いて作製した直径10mm,長さ40mmの円筒
を骨欠損部に挿入した。ついで，この円筒中に実施例１
で用いたのと同じハイドロキシアパタイトの顆粒を十分
充填し，上部粘膜面を縫合した。On the other hand, the 4th premolar of the upper jaw of a beagle dog was extracted, and at 50 or more days after the extraction, a vertical bone defect was created for the 4th premolar. After exposing the root surface and the bone surface, a cylinder with a diameter of 10 mm and a length of 40 mm made of the above non-woven fabric was inserted into the bone defect. Then, in this cylinder, Example 1 was placed.
The same granules of hydroxyapatite as used in Section 2 above were fully filled and the upper mucosal surface was sutured.
３カ月後の観察では，ハイドロキシアパタイト面は上方
からの圧迫でも安定した面を保ち，ハイドロキシアパタ
イトが生体内で正常な形で固定され，良好な歯周支持組
織を生成していることが認められた。Three months later, it was confirmed that the hydroxyapatite surface remained stable even when pressed from above, and the hydroxyapatite was fixed in a normal form in the living body, producing good periodontal support tissue. It was
実施例３ キトサン粉末（新日本化学製，脱アセチル化度87％）
を,0.5w/w％の酢酸水溶液に溶解し，濃度が２％のキト
サンドープを得た。このドープ100gに,20gのトリカルシ
ウムホスフエート（ジヨンソン・エンド・ジヨンソン社
製）を加えたのち，長さが5mmの円筒に成形し，ついで1
N−NaOHで中和し，凝固した。凝固物を，温水で十分に
洗浄した後，乾燥して円筒を得た。Example 3 Chitosan powder (manufactured by Shin Nippon Chemical Co., Ltd., deacetylation degree 87%)
Was dissolved in 0.5 w / w% acetic acid aqueous solution to obtain a chitosan dope having a concentration of 2%. To 100 g of this dope, 20 g of tricalcium phosphate (manufactured by Jyonson End Jyonson) was added, and then molded into a cylinder with a length of 5 mm.
It was neutralized with N-NaOH and solidified. The solidified product was thoroughly washed with warm water and then dried to obtain a cylinder.
一方，ビーグル犬の下顎の第４小臼歯を抜歯し,50日間
経過後，上記円筒を歯根面に7mmの長さで充填し，さら
に，骨膜弁で縫合固定した。４カ月後の観察では，充填
部に強固な骨組織が形成されていた。On the other hand, the fourth premolar of the lower jaw of a beagle dog was extracted, and after 50 days, the root surface was filled with the above cylinder with a length of 7 mm and further fixed with a periosteal flap. Upon observation 4 months later, strong bone tissue was formed in the filling part.
（発明の効果） 本発明の移植材は，例えば，歯周骨欠損等の硬組織の移
植材として使用した場合，結合組織の生成がスムーズと
なり，新生骨も生成しやすい環境になり，移植材の本来
の目的である安定したリン酸カルシウム化合物の生体へ
の固定が行われ，良好な歯周支持組織等の生成が可能で
ある等，生体用移植材として優れた性能を有する。(Effects of the Invention) When the transplant material of the present invention is used as a transplant material for hard tissue such as periodontal bone defect, for example, the generation of connective tissue is smooth, and an environment in which new bone is easily generated is created. The stable calcium phosphate compound is fixed to the living body, which is the original purpose of the above method, and excellent periodontal supporting tissue can be generated.
Claims (1)
酸カルシウム化合物がキチンで固定されるか又はキチン
で包囲されたことを特徴とする生体用移植材。1. A transplant material for living body, characterized in that powdery, granular or block-like calcium phosphate compounds are fixed with chitin or surrounded by chitin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63006564A JPH0767487B2 (en) | 1988-01-14 | 1988-01-14 | Living body transplant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63006564A JPH0767487B2 (en) | 1988-01-14 | 1988-01-14 | Living body transplant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01181872A JPH01181872A (en) | 1989-07-19 |
| JPH0767487B2 true JPH0767487B2 (en) | 1995-07-26 |
Family
ID=11641831
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63006564A Expired - Fee Related JPH0767487B2 (en) | 1988-01-14 | 1988-01-14 | Living body transplant |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0767487B2 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0277262A (en) * | 1988-09-13 | 1990-03-16 | Sunstar Inc | Substrate material for hard biotissue |
| JPH02118516U (en) * | 1989-03-14 | 1990-09-25 | ||
| JPH02277451A (en) * | 1989-04-19 | 1990-11-14 | Hanna Sangyo Kk | Dental implant |
| EP0416398A1 (en) * | 1989-08-24 | 1991-03-13 | Asahi Kogaku Kogyo Kabushiki Kaisha | Paste for bonding granular bone prosthesis and bone prosthesis using same |
| JP3931134B2 (en) * | 2002-10-31 | 2007-06-13 | 泰彦 田畑 | Implant for living tissue regeneration and method for producing the same |
| JP2006299459A (en) * | 2005-04-20 | 2006-11-02 | National Institute For Materials Science | Method for producing biodegradable polymer non-woven tube |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62202884A (en) * | 1986-02-28 | 1987-09-07 | 工業技術院長 | Live body substitute ceramic material |
| JPS62221358A (en) * | 1986-03-20 | 1987-09-29 | 東燃株式会社 | Filling material for bone defects and voids |
| JPS6434372A (en) * | 1987-07-31 | 1989-02-03 | Toa Nenryo Kogyo Kk | Filler for bone depleted part and void part |
-
1988
- 1988-01-14 JP JP63006564A patent/JPH0767487B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH01181872A (en) | 1989-07-19 |
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