JPH0760220B2 - Inverted microscope with manipulator - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to an inverted microscope with a manipulator including a micromanipulator for performing various fine operations on an object to be observed of a microscope.

2. Description of the Related Art A conventional inverted microscope with a manipulator of this type includes a stage 1 and a stage 1 as shown in FIG.
A culture vessel 2 placed on top and having cells 2a stored therein
A condenser lens 3 provided above the stage 1 and including a ring slit 3a therein, and the stage 1
An objective lens 5 mounted on a revolver 4 provided below the lens and having a phase plate 5a therein, and a glass needle 7a fixed to an end portion of the lower surface side of the stage 1 through a mounting bracket 6.
Is a cell 2a in the culture vessel 2, that is, a micromanipulator 7 extending near the focal position of the microscope in this case.
And the like, the cells 2a in the culture container 2 are pierced with the glass needle 7a of the micromanipulator 7 so that the enzyme or the like in the culture solution can penetrate into the cells 2a.
It was configured so that a so-called pricking procedure could be performed.

However, in the case of this conventional example, since the plurality of operation portions 7b of the manipulator 7 are mechanically interlocked, the interlocking mechanism structure is complicated, and the reliability and durability of the device are low. On the other hand, on the other hand, there is also a problem that the operability is inevitably inferior because the positions of the respective operation parts 7b are restricted respectively.

Further, in general, since the bottom surface of the culture container 2 and the surface of the cultured cells are not uniform, when the tip of the glass needle 7a is moved down to the focus position, the container tip of the glass needle 7a is used. There is a problem that the glass needle 7a itself is often damaged due to the accidental puncture of the bottom surface of the glass, and as a result, the efficiency of pricking is reduced.

The present invention has been made in order to improve the above-mentioned conventional problems, the object is to have a high reliability and durability in the device, and also excellent operability, the pricking operation of There is no fear of damaging the glass needle,
An object of the present invention is to provide an inverted microscope with a manipulator capable of dramatically improving the work efficiency of the pricking operation.

An inverted microscope with a manipulator of the present invention is a needle holder for holding a glass needle in an inverted microscope with a manipulator equipped with a micromanipulator for performing various fine operations on an object to be observed, and the needle holder. A hydraulic drive unit that supports and that drives the vertical movement along the optical axis, a focusing handle that adjusts the correlation distance between the observed object and the objective lens, and a hydraulic transmission tube between the needle holder. Hydraulic connection tube connected between the needle holder and a first hydraulic operating portion that is connected to the focusing handle and operates the needle holder by an amount corresponding to the amount of rotation of the focusing handle. And a second hydraulic operating portion that is connected via the second hydraulic actuator and that operates the needle holder.

Embodiment Hereinafter, an embodiment of the present invention will be described in detail with reference to FIGS. 1 to 12.

1 to 9 show an embodiment of the present invention.

Here, FIG. 1 and FIG. 2 are a side view and a front view showing a schematic configuration of an inverted microscope with a manipulator to which the present embodiment is applied, respectively.

In the configuration of the embodiment shown in FIGS. 1 and 2,
10 is a microscope main body, 11 is an illumination system column of the microscope main body 10, 12
In this case, for example,
As described in Japanese Patent No. 58-137655, the stage is detachably fixed by a combined structure of a female dovetail portion and a male dovetail portion.

Further, 13 is a condenser holder attached to the illumination system support 11 above the stage 12, 14 is a condenser lens supported by the condenser holder 13, and 15 is a space between the stage 12 and the condenser lens 14. Is provided with a centering mechanism described later on the optical axis, and holds the glass needle 16 in a suspended state on the optical axis, 17 is fixed by screwing on the upper surface of the stage 12 The needle holder 15 is detachably supported and can be driven in the vertical direction along the optical axis. In this case, for example, a hydraulic drive unit as described in JP-B-57-28122 is used. Shown at 18 is the outside of the microscope body 10, and the glass needle is provided via a hydraulic transmission tube 19.
An injector that is connected to the hydraulic transmission tube 19 and fills the hydraulic transmission tube 19 with silicone oil or the like to apply pressure to the chemical solution or the like in the glass needle 16 to push out the chemical solution from the needle tip.

Further, 20 is below the stage 12 and is the main body of the microscope.
Reference numeral 10 denotes a revolver mounted vertically movable, reference numeral 21 denotes an objective lens fixed to the revolver 20, and reference numeral 22 denotes a rotatably pivotable member on each of the left and right sides of the microscope body 10 as shown in FIG. In this case, for example, there are a pair of focusing handles connected to the revolver 20 via a coarse and fine adjustment device as described in Japanese Patent Publication No. 52-43688, and 23 is the microscope main body by a mounting screw 24. It is detachably fixed to 10 and its operating part is connected to the pair of focusing handles 22 and 2 described above.
The fine movement handle 22a is connected to one of the fine movement handles 22a.
Driven by 22a, for example, Japanese Examined Patent Publication Sho 57-2
A first hydraulic operating unit as described in Japanese Patent No. 8122, 25
Is a hydraulic pressure transmission tube connecting between the first hydraulic operating unit 23 and the hydraulic drive unit 17, and 26 is the first hydraulic operating unit 23.
Is a second hydraulic operating section connected to the hydraulic pressure transmitting tube 27 via the hydraulic transmission tube 27.

Subsequently, the configuration of each of the above-mentioned respective parts will be described in more detail.

First, FIGS. 3 and 4 are a partially cutaway plan view and a vertical sectional view, respectively, showing the detailed structure of the needle holder 15 in the same embodiment.

The needle holder 15 shown in FIG. 3 and FIG.
In the configuration of 28, 28 is the male dovetail portion 28a
7 is an annular support frame that is fitted to the female dovetail portion and is fixed in a vertically adjustable manner by tightening the clamp screw 29. With respect to the support frame 28, one set of each spring is provided on one half side in the circumferential direction. 31 and 31 are screwed together, and a pair of centering knobs 32 and 32 are screwed to face the spring sets 31 and 31 on the other half side so as to be able to advance and retract. Further, 30 is an annular centering frame which is movably inserted in the support frame 28 in a plane orthogonal to the optical axis, and the centering frame 30 is always a central portion by the spring sets 31, 31. That is, while being pressed in the optical axis direction, the centering knobs 32, 32 can be centered by screwing back and forth. Further, 33 is fixed to the centering frame 30 by being inserted into the centering frame 30 and penetrating the support frame 28, and fixed by tightening a fixing screw 34 screwed to the centering frame 30, and released from the centering frame 30 by loosening it. The annular holder frame 35, which is made possible to do so, holds the base side of the holder frame 33 in a horizontal state, and extends the distal end portion of the center portion of the holder frame 33 that extends downward on the optical axis. The L-shaped holder has the hydraulic transmission tube 19 connected to the base and the glass needle 16 connected and held to the tip by a clamp screw 36.

Next, FIG. 5 and FIG. 6 are hydraulic drive units in the same embodiment.
FIG. 17 is a partially cutaway plan view and a vertical cross-sectional view showing a detailed configuration of 17, respectively.

In the construction of the hydraulic drive unit 17 shown in FIGS. 5 and 6, 37 denotes a mounting base fixed to the upper surface of the stage 12 with a screw to form a female dovetail portion 37a in the vertical direction, and 38 denotes the mounting base. The mounting base 37 is rotatably pivoted laterally, has a slip-out preventing head 38a at one end, and has a flat spring 39 on the peripheral side, and a coarse movement handle 40 is screwed at the other end. When the pinion shaft 38 is attached and the pinion shaft 38 is fixed and the coarse movement handle 40 is rotated in the screwing direction, the flat spring 39 is compressed to generate a large repulsive force, and this repulsive force is utilized. The operation force for integrally rotating the pinion shaft 38 and the coarse movement handle 40 can be adjusted. In this case, the pinion shaft 38 and the coarse movement handle 40 are adjusted.
In order to be able to set the frictional resistance force of the Sarabana 39 acting between the pinion shaft 38 and the
It does not rotate and loosen.

Further, 41 is supported by the mounting base 37 so as to be vertically movable by a male dovetail portion 42 that fits with the female dovetail portion 37a,
Reference numeral 44 denotes a cylinder that is vertically moved by the rotation operation of the coarse movement handle 40 via the pinion shaft 38 and a rack 43 meshed with the pinion shaft 38, and 44 denotes an oil chamber 41a and an empty chamber in the cylinder 41. 41b is a rolling diaphragm, 45 is a piston rod whose one end is fixed to the rolling diaphragm 44 from the vacant chamber 41b side, and 46 is mounted on the cylinder 41 so as to be vertically movable via a roller guide 47. , And the female dovetail portion 46a for fitting the male dovetail portion 28a of the support frame 28 in the needle holder 15 in the vertical direction of the back with a slide base having the other end portion of the piston rod 45 fixed to the bent lower end portion. Has been formed.

Next, FIG. 7 is a vertical cross-sectional view showing the detailed structure of the first hydraulic operating portion 23 in the embodiment.

In the configuration of the first hydraulic operating unit 23 shown in FIG. 7, reference numeral 48 is the microscope main body coaxial with the focusing handle 22.
A frame having a partially cylindrical shape in which one end side is fitted and detachably fixed to the mounting ring 49 provided in 10 by the mounting screw 24, and 50 is fixed to the other end side of the frame 49. The head 51 is pivotally supported in the frame 48 coaxially with the focusing handle 22, and is located at one end side and corresponds to the one side focusing handle 22 made of the pair, which is a fine movement handle 22a. Is a transmission shaft in which a transmission drum 52 made of an elastic material such as rubber that is press-fitted with is fixed, and a transmission drum 51b with a key groove 51a parallel to the axial direction is integrally formed at the other end side. .

Further, 54 is a cylinder screwed to the head 50, and 55 is screwed from the inner end side of the cylinder 54 coaxially with the transmission shaft 51 so as to be able to advance and retract in the axial direction, and protrudes to the outer peripheral portion. A screw shaft in which a pin 56 is slid into the key groove 51a, 57 is a rolling diaphragm that divides the inside of the cylinder 54 into an oil chamber 54a and an empty chamber 54b, and 58 is the other end side fixed to the rolling diaphragm 57. The piston rod has one end side fitted into the center hole of the screw shaft 55 and brought into contact with the inner bottom portion.

Then, in this case, one rotation operation of the fine movement handle 22a corresponding to the other focusing handle 22 on the side on which the first hydraulic operating portion 23 is not mounted, out of the pair of focusing handles 22. , The objective lens 21 is, for example, 20
On the other hand, the rotation of the fine movement handle 22a is converted into the axial movement of the piston rod 58 via the transmission drum 52, the transmission shaft 51, and the screw shaft 55, respectively. As a result, the rolling diaphragm 57 is moved, and as a result, the hydraulic pressure corresponding to the amount of rotation of the fine movement handle 22a and thus the amount of movement of the objective lens 21 is transferred to the hydraulic drive unit 17 via the hydraulic transmission tube 25. The needle holder 15 is moved in the same direction as the movement amount of the objective lens 21, that is, moved in focus, by being provided in the cylinder 41 of the.

Further, at this time, if there is no connection between the objective lens 21 and the glass needle 16 and only the atmospheric layer is simply interposed, the operation in this relevant part is performed in this way. However, the cells to be subjected to the intended operation are usually contained in a liquid such as a culture medium or water, and when the focus position is moved in the liquid, the optical path length thereof is changed, resulting in Therefore, the working distance of the objective lens 21 also changes, and the focus of the tip of the glass needle 16 initially set to be in focus is deviated.

Here, if the change in the optical path length in the liquid is Δl,
Δl / n (n is the refractive index of the liquid) corresponds to the change in the working distance of the objective lens 21, and in order to compensate for this error,
The screw pitch of the screw shaft 55 may be selected so that the ratio of the amount of movement of the objective lens 21 by the fine movement handle 22a to the amount of movement of the needle holder 15 is near the refractive index n of the liquid. Then the problem of out of focus is solved.

Next, FIG. 8 is a vertical cross-sectional view showing a detailed configuration of the second hydraulic operating portion 26 in the embodiment.

In the structure of the second hydraulic operating unit 26 shown in FIG. 8, 59 is a base, 60 is fixed on the base 59 via a support 61, and each inner end also serves as a guide at the center. It shows a hand rest in which a long groove 60a having a portion as a stopper portion is formed.

Further, 62 is a cylindrical frame fixed on the base 59, 63 is a cylinder fixed to the lower part of the side wall of the frame 62, and 64 is an inside of the cylinder 63 divided into an oil chamber 63a and an empty chamber 63b. A rolling diaphragm, 65 is a piston rod whose one end is fixed to the rolling diaphragm 64, 66 is arranged on the base 59 in the frame 62, and the horizontal direction,
Here, the slider is slidable in the direction of arrow A in FIG. 8, the other end of the piston rod 65 is coupled, and a slider 66a having a spherical head is provided on the upper portion, and 67 is the frame 62. Is a support frame that is screwed to the top wall portion so that the vertical position can be adjusted.

Further, 68 is a ball which is swingably supported with respect to the support frame 67 and has a spherical concave portion 68a which is swingably fitted to the spherical head portion of the lever 66a at the lower portion, and 69 is a ball of the ball 68. A cylinder integrally fixed to the upper portion, 70 is a fine movement joystick in which the lower end side is fixed to the upper end portion of the cylinder 69, and the outer peripheral portion slightly above the lower end portion is the long groove.
Guided in the direction of arrow B in FIG. 8 by 60a,
The swinging range is regulated by using the inner ends of the long grooves 60a as stoppers, and in particular, the lower limit position of the needle holder 15 can be set when the tip of the glass needle 16 is in focus. Here, by the swinging operation of the fine movement joystick 70 with the ball 68 as the center of rotation, the slider 66 and thus the piston rod 65 are slidably moved in the axial direction corresponding to the swing amount. I'm supposed to get it.

Further, 71 is a rolling diaphragm that divides the inside of the cylinder 69 into an oil chamber 69a and an empty chamber 69b, and 72 is each of the oil chambers 63a, 69a.
a hydraulic transmission tube connecting between a, 73 is a piston rod whose lower end is fixed to the rolling diaphragm 71, 74 is on the upper end side of the fine movement joystick 70, with respect to the upper end of the piston rod 73, Around the central axis via a screw shaft (not shown), here the fine movement joystick 70
It is mounted so as to be screwed back and forth in the direction of arrow C in FIG. 8 by being rotated around the shaft, and the piston rod 73 is axially moved by the screwing back and forth turning operation in correspondence with the amount of screwing back and forth. It is a coarse-motion knob that can be turned on.

Next, FIG. 9 is a schematic explanatory view showing the overall configuration of each hydraulic drive system in the embodiment.

As shown in FIG. 9, in each hydraulic drive system, the hydraulic pressure generating portions in the first and second hydraulic pressure operating portions 23 and 26 are configured to communicate with each other, and the needle holder 15
Is designed to be able to drive and control the hydraulic drive unit 17, and in this case, for example, the hydraulic drive unit 17 has a cylinder capacity of a stroke of 12 mm. In order to prevent the total stroke of the hydraulic operating portions 23 and 26 of 2 from exceeding 12 mm, a stopper portion is set at the inner end portion of the key groove 51a with which the pin 56 is slid, and the respective rolling diaphragms. It is possible to prevent damage to 57 (corresponding to the first hydraulic operating unit 23) and 64, 71 (corresponding to the second hydraulic operating unit 26).

In the case of the present embodiment, the first and second hydraulic operating parts
Although 23 and 26 are connected in series to the hydraulic drive unit 17, they may be connected in parallel.

Also, within a range that does not exceed the stroke of the hydraulic drive unit 17,
If the total strokes of the first and second hydraulic operating portions 23 and 26 are set, any other required hydraulic operating portions may be arranged in accordance with this.

Further, in the case of the present embodiment, the first hydraulic operating portion 23 which is linked with the focusing is provided with the fine movement handle 22a of the focusing handle 22.
However, the invention is not limited to this, and may be directly connected to the vertical movement mechanism of the objective lens 21 and the revolver 20.

The inverted microscope with a manipulator according to the present embodiment is configured as described above and operates as follows.

That is, first, for the sample placed on the stage 12,
After the focusing operation is performed by using the focusing handle 22, the first hydraulic operating unit 23 is connected to the fine movement handle 22a of the focusing handle 22.

Next, the holder of the holder frame 33 in the needle holder 15
Although the setting operation of the glass needle 16 to the 35 is started, at first, the condenser lens 14 interferes, so that it is bounced upwards or removed, and the hydraulic drive unit 17 is coarse. The moving handle 40 is operated to raise the support frame 28 of the needle holder 15 sufficiently upward.

Then, in this state, while attaching the glass needle 16 of an appropriate length to the holder 35 of the holder frame 33,
33 is fitted in the centering frame 30 and fixed by the fixing screw 34, and further, the coarse movement handle 40 and the second hydraulic operating portion 26
The needle holder 15 is moved down and the tip of the glass needle 16 is moved slightly above the focus position by operating the coarse adjustment knob 74 together with, but in this case, for example, 4 × to 10 ×. When the objective lens 21 is used, the tip of the glass needle 16 appears as a white dot in the visual field.

Here, the position of the centering frame 30 that is constantly pressed by another set of spring sets 31, 31 by screwing back and forth one set of centering knobs 32, 32 in the needle holder 15 respectively. Adjustment, that is, centering of the glass needle 16 on the optical axis, and thereby the glass needle 16
Is set on the optical axis of the central part of the visual field. Furthermore, in this case, it is more accurate and effective if the glass needle 16 is set on the optical axis by using the cross scale of the eyepiece.

After that, the fine movement joystick 70 in the second hydraulic operating portion 26 is slowly rocked to be brought down to the position of the lower limit stopper portion set at the inner end portion of the long groove 60a, and further, in that state. Then, the coarse movement knob 74 is rotated to lower the glass needle 16, and the tip of the glass needle 16 is aligned with the focus position, whereby the setting of the entire apparatus is completed.

Subsequently, a pricking operation for the cultured cells of the sample is started. In the case of this embodiment, by swinging the fine joystick 70, for example, with the focus position at the lower limit of about 100 to 500 μm. The glass needle 16 can be moved up and down. Then, the fine movement handle 22a is screwed back and forth to correct the focus position, so that the movement of the fine movement handle 22a is changed from the second hydraulic pressure operation portion 26 to the first
After being passed through the hydraulic operation unit 23, the hydraulic drive unit 17 is transmitted to the hydraulic drive unit 17, and the hydraulic drive unit 17 is also interlocked with the lower limit position of the needle holder 15, and thus the glass needle 16.

Therefore, in such an operation mode, when performing the pricking operation, even if the bottom surface of the culture vessel or the surface of the cultured cells is not a uniform flat surface, the cell is always moved to a position where the cells are in focus. Since the needle tip of the glass needle 16 descends, unlike the conventional case, the pricking can be performed satisfactorily and accurately, and by accidentally piercing the bottom surface of the container with the needle tip. Further, it is possible to effectively prevent the glass needle 16 from being damaged, and as a result, the pricking operation can be executed one after another.

Further, in this case, since the glass needle 16 is held by the annular needle holder 15 via the annular holder 35, the phase difference spectroscope between the condenser lens 14 and the sample is damaged. There is no fear.

Next, FIG. 10 and FIG. 11 are a partially cutaway plan view and a longitudinal sectional view, respectively, showing the detailed structure of the needle holder 15 according to another embodiment.

The structure of the needle holder 15 according to another embodiment shown in FIGS. 10 and 11 is the holder frame in the above embodiment.
Instead of 33, the transparent disk 75 is attached to the centering frame 30, and then the base of the holder 35 is held in the central part of the transparent disk 75. In this case, The length from the held portion (base portion) of the holder 35 to the tip of the glass needle 16 can be shortened, and in this case also, in order to hold the holder 35 by the transparent disk 75, the condenser lens 14 There is no fear of spoiling the phase-contrast microscope between the sample and the specimen.

Next, FIGS. 12 (A) and (B) show the second embodiment according to another embodiment.
FIG. 3 is a cross-sectional view and a side view of a fine movement handle showing a detailed configuration of the hydraulic operating unit 26.

The configuration of the second hydraulic operating portion 26 according to another embodiment shown in FIGS. 12A and 12B is such that the fine movement joystick 70 and the coarse movement knob 74 in the above embodiment are replaced by fine movement. A cylinder 76 and a cylinder 69 are driven by using a handle 76 and a screw shaft 77, and a coarse movement handle 78 and a screw shaft 79, and a pin fixed to the cylinder 63 side is attached to the fine movement handle 76. The long groove 76a for sliding the 80 together is provided, and the inner edge of the long groove 76a is used as the stopper portion in the same manner as described above, and the same effect can be obtained.

EFFECTS OF THE INVENTION As described above in detail with reference to the embodiments, according to the inverted microscope with the manipulator of the present invention, the hydraulic drive section holding the needle holder is provided with the first and second hydraulic operation sections. Since the hydraulic drive unit, and eventually the glass needle of the needle holder, can be operated up and down by simply connecting by means of the above, the structure of the interlocking mechanism is extremely simplified, and as a result, the reliability and durability of the device can be improved. . On the other hand, since the hydraulic operation unit is connected to the hydraulic drive unit via the hydraulic transmission tube in this way, as a result, the hydraulic operation unit can be set at a free position with respect to the microscope main body.
A structure with excellent operability can be obtained.

Further, since the first hydraulic operating unit is connected to the focusing handle for adjusting the correlation distance between the object to be observed and the objective lens,
The glass needle of the needle holder can be interlocked in accordance with the turning operation amount of the focusing handle, and further, the focusing movement amount of the objective lens, and at the same time, a member for compensating a change in optical path length in the liquid is provided. Thus, when transmitting the turning operation of the focusing handle, the objective lens can be effectively and appropriately focused by the focusing handle in a liquid such as a culture solution.

Furthermore, even if the bottom surface of the culture vessel or the surface of the cultured cells is not a flat surface, if the lower limit position of the tip of the glass needle is set to the focus position, the tip of the glass needle will be lowered when the glass needle is lowered. The part always stops at the focus position, and even if the focus is adjusted again, the tip will always stop at the focus position, so there is no fear of damaging the glass needle during pricking operation, The work efficiency of the pricking is dramatically improved.

[Brief description of drawings]

1 and 2 are a side view and a front view respectively showing a schematic configuration of an inverted microscope with a manipulator to which an embodiment of the present invention is applied, and FIGS. 3 and 4 show the embodiment. FIG. 5 is a partially cutaway plan view and a vertical sectional view showing the detailed structure of the needle holder in FIG. 5, and FIG. 5 and FIG. 6 are partially cutaway plan views and a vertical cross section showing the detailed structure of the hydraulic drive unit in the embodiment. FIGS. 7 and 8 are longitudinal sectional views showing the detailed structure of the first hydraulic operating part in the embodiment, and FIG. 8 is a longitudinal sectional view showing the detailed structure of the second hydraulic operating part in the embodiment. The drawing is a schematic explanatory view showing the overall construction of the hydraulic drive system in the above-mentioned embodiment. 10 and 11 are a partially cutaway plan view and a vertical sectional view, respectively, showing the detailed structure of the needle holder according to another embodiment, and FIGS. 12 (A) and 12 (B). FIG. 6 is a cross-sectional view and a side view of a fine movement handle showing a detailed configuration of the second hydraulic operating unit according to another embodiment, respectively. Further, FIG. 13 is a partially cutaway front view showing the configuration of the main part of an inverted microscope with a manipulator according to a conventional example. 10 …… Microscope body, 11 …… Illumination support, 12 …… Stage, 13 …… Condenser holder, 14 …… Condenser lens, 15 …… Needle holder, 16 …… Glass needle, 17 …… Hydraulic drive, 18 …… Injector, 19,25,27,72 …… Hydraulic transmission tube, 20 …… Revolver, 21 …… Objective lens. 22 ... Focusing handle, 22a ... Fine movement handle, 23 ... First hydraulic operating part, 26 ... Second hydraulic operating part, 28 ... Support frame, 29 ... Clamp screw, 30 ... Centering Frame, 31 …… Spring set, 32 …… Centering knob, 33 …… Holder frame, 34 ……
Fixing screw, 35 …… holder, 36 …… clamp screw. 37 …… Mounting stand, 38 …… Pinion shaft, 39 …… Sarabane,
40 …… Coarse handle, 41,54,63,69 …… Cylinder, 41a.5
4a, 63a, 69a ... Oil chamber, 41b, 54b, 63b, 69b ... Vacant chamber, 43 ...
… Rack, 44,57,64,71 …… Rolling diaphragm, 4
5,58,65,73 …… Piston rod, 46 …… Slide base, 47 ……
Roller guide, 48, 62 …… frame, 49 …… mounting ring, 50 …… head, 51 …… transmission shaft, 51a …… keyway, 55
…… Screw axis (compensation member for optical path length change), 56 …… pin, 59
...... Base, 60 ...... Hand rest, 60a ...... Long groove (having a stopper part for lower limit position regulation at one inner end), 61 ......
Prop, 66 …… Slider, 66a …… Lever, 67 …… Support frame, 68 …… Ball, 68a …… Recess, 70 …… Fine movement joystick (main part of operation handle), 74 …… Coarse control (operation) Part of the handle). 75 …… Transparent disk, 76 …… Fine movement handle, 76a …… Long groove (stopper part), 77,79 …… Screw shaft, 78 …… Coarse movement handle, 80 …… Pin.

Front Page Continuation (72) Inventor Katsura Motomura 43-2 2 Hatagaya, Shibuya-ku, Tokyo Olympus Optical Industry Co., Ltd. (72) Inventor Yasuo Inoue 43-2 2 Hatagaya, Shibuya-ku, Tokyo Olympus Optical Industry Incorporated (72) Inventor Eiichi Narimo 4-27-9 Minamikarasuyama, Setagaya-ku, Tokyo Inside the Shigeshi Institute of Scientific Research, Inc. (72) Inventor Shinji Yoneyama 4-27-9 Minamikarasuyama, Setagaya-ku, Tokyo No. Co., Ltd. Narishige Science Research Institute

Claims (3)

[Claims] 1. An inverted microscope with a manipulator equipped with a micromanipulator for performing various fine operations on an object to be observed, a needle holder for holding a glass needle, and a needle holder for supporting the needle holder. Is vertically connected along an optical axis, a focusing handle for adjusting the correlation distance between the object to be observed and the objective lens, and the needle holder, which are connected via a hydraulic transmission tube, and Connected via a hydraulic pressure transmission tube between the needle holder and a first hydraulic operating unit that operates the needle holder by an amount corresponding to the amount of rotation of the focusing handle in conjunction with the focusing handle; An inverted microscope with a manipulator, characterized in that it is provided with a second hydraulic operating section for operating the needle holder. 2. The first hydraulic operation unit compensates for a change in optical path length in a liquid such as a culture liquid in the object to be observed when focusing is performed by the focusing handle in the liquid. The inverted microscope with a manipulator according to claim (1), wherein the turning operation of the focusing handle is transmitted via a member. 3. The second hydraulic operating section is provided with a stopper section for restricting an operating amount of an operating handle for adjusting an operating hydraulic pressure at a lower limit position of the needle holder. The inverted microscope with a manipulator described in 1).