JPH0737365B2 - Pest control method - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for controlling harmful insects by microorganisms, and more particularly to a method for utilizing natural enemy microorganisms suitable for controlling scarab beetles generated on lawns.

[0002]

2. Description of the Related Art Conventionally, a large amount of various chemical pesticides have been used for controlling pests on lawns, which causes industrial hygiene problems for golf course employees such as caddies, and also causes rainwater to leak from golf courses. Environmental pollution by chemical pesticides, water sources such as drinking water are contaminated with pesticides, and they pose a problem as a source of various pollution such as affecting the human body.

In order to deal with these problems, various pest control methods that do not use chemical pesticides have been studied. Among them, attempts have been made to collect pests by using traps, sex pheromones, etc., but some results have been achieved, but none of them could prevent the damage caused by larvae of scarab beetles on the lawn.

[0004]

[Problems to be Solved by the Invention] The purpose of controlling the scarab beetle larvae occurring on the lawn using microorganisms is to prevent environmental pollution by chemical pesticides, and is suitable for controlling the scarab beetle larvae, and golf. It was an important issue to find a method that can mechanically spray a large amount in the field.

Conventionally, as a natural enemy to insects, in order to control pests of mulberry trees, filamentous fungi such as Beauveria fungus are cultured and attached to a solid medium such as sponge-shaped bran pellets (Japanese Patent Laid-Open No. 63-148980). Te (Tokuhei 4-4866
No.), a method of spraying this around the mulberry tree has been tried.

However, such a method cannot control the substances existing in the soil such as the larvae of scarab beetles.

[0007]

In order to solve the problems of the present invention, it is necessary to select a microorganism that is harmless to the scarab beetle larvae and is strong against the outdoor environment. As a result of a comparative study on various microorganisms in order to achieve these objects, it was found that a certain kind of metalridium bacterium has a great insecticidal effect especially on the larvae of scarab beetles.

In the present invention, of the metalridium bacteria, metalridium anisopree bacterium is used, and particularly preferred is Metalridium anisopree No. 31 strain (Microtechnology Research Institute No. 12729) whose insecticidal ability is enhanced by protoplast formation and ultraviolet irradiation. To use.

[0009] This was inoculated into spongy bran sponge pellets of about 10 to 20 mm and tried to be sprayed on the lawn, but it was unsuitable for a large amount of mechanical spraying, and it was as vast as a lawn at a golf course. Difficult to spread evenly in different areas,
Also, we know that pellets of about 10 to 20 mm require a very large amount of formulation, resulting in a very high cost, and that large particles scattered around the green of the golf course are a great obstacle to golf play. It was

[0010] For this purpose, it was noted that the medium mainly consisting of bran cells to which the metalridium anisoprie bacterium was adhered by culturing was crushed to some extent into granules having a specific particle size. The following particle sizes, preferably
We have found a method for controlling pests by spraying granular fusuma powder with culture-attached metalridium bacteria having a particle size of 0.5 mm to 2.0 mm.

[0011] Particularly, as a solid medium mainly composed of bran, which is adhered by culturing Metaridium anisopree bacterium, bran pellets formed in granular form (sponge-like bran pellets)
That use. Examples of the fusuma pellets formed into the granular shape are, for example, Japanese Patent Publication No. 1-29554 and JP-A-64-63.
Examples include pellets formed by using Buma as a main raw material by various methods other than the method disclosed in Japanese Patent No. 371. In the method of the present invention, the use of granulated fusuma pellets allows the culture of Metaridium anisoprie bacterium to be uniformly and efficiently adhered to the culture.

[0012] The solid culture medium mainly composed of branula, which is obtained by culturing and adhering the Metaridium anisoprie bacterium thus obtained, can be further pulverized to give granular bran powder having a relatively uniform particle size. When this solid medium is crushed, the spores of metalridium bacteria become fine powder and scatter into the air, resulting in the loss of bacterial cells and spores and the loss of bacteria.
Activity is likely to occur. The following method is used to prevent this .

That is, in carrying out the method of the present invention, water-insoluble sticky substances such as liquid paraffin and oils and fats are added to a solid culture medium mainly composed of branella to which culture-attached metalridium anisoprie bacterium is attached to maintain the activity of the bacterium. This is to use granular fusuma powder having a particle size of 3 mm or less crushed.

Examples of the water-insoluble sticky substance in the present invention include oils and fats such as liquid paraffin, vegetable oils and animal oils, and liquid paraffin and vegetable oils are particularly preferable. The pulverization can be made easier by drying the solid medium to some extent before or after the addition of the water-insoluble sticky substance in the present invention. This drying may be carried out within a normal temperature range in which bacteria are not killed, depending on the pulverization method, and is usually about 2
It takes about 2 days to dry naturally at around 0 ° C, but 30 to 50 ° C
More preferably, the water content is dried to about 20 to 10%. The amount of the water-insoluble sticky substance added in the present invention may be such that the medium and spores do not scatter, and usually 1 to 5% by weight (to the medium) is suitable.

In the present invention, the solid medium mainly composed of bran cells to which the metalridium anisoprie bacterium is adhered by culture is used.
As a method for producing a granular bran powder having a particle size of mm or less, other than the above method, without adding a water-insoluble sticky substance, it is pulverized in a semi-dry state (as long as spores are not significantly scattered), After molding with a pellet molding machine, there is also a method of drying, and adding a sticky substance other than the water-insoluble sticky substance (for example, dextrin or other water-soluble glue, surfactant), etc., and crushing under the conditions similar to the above There is also a method of drying.

[0016]

EXAMPLES The present invention will be described below with reference to examples.

B) 100 parts by weight of wheat bran and 10 parts by weight of wheat flour were dissolved in 80 parts by weight of water and heat-gelatinized, kneaded, and extruded by a pellet machine to form 8 mmφ ×
25 mm was dried in a drying room at 80 ° C. for 2 hours to obtain spongy bran pellets having a water content of 10%.

B) Metalridium anisopree No. 31
Bacterial strain (Metarhizium anisopliae No.31: Microtechnology Research Institute No. 12729) was pre-cultured in SDY liquid medium in advance, and then b)
By culturing for 2 weeks with the bran pellets prepared by the above method (water sterilization to 65% water content), a solid medium mainly composed of bran cells adhered with the Metaridium anisoprie bacterium was adhered.

C) The solid culture medium prepared by the above-mentioned method (b) and on which the Metaridium anisoprie bacterium has been adhered by culture was subjected to the following method to obtain the granular bran powder of the present invention having a particle size of about 1 to 2 mm.

Sample (1) Granular bran powder obtained by adding 10 cc of liquid paraffin to 200 g of the solid medium prepared by the method (2) and drying and grinding.

Sample (2) Dried solid medium prepared by the method (2) above 20
Granular bran powder obtained by adding 5 cc of liquid paraffin to 0 g.

Sample (3) A dried solid medium prepared by the method of the above (b) 20
Granular bran powder obtained by adding 5 cc of vegetable oil (salad oil) to 0 g and crushing.

Sample (4) Dried solid medium prepared by the method (b) above 20
0g vegetable oil (salad oil) 8cc and surfactant Twin 80
(Polyoxyethylene (20) sorbitan monooleate) Granular fusuma powder obtained by adding a small amount and pulverizing.

Sample (5) A granular fusuma powder obtained by adding 8cc of liquid paraffin and a small amount of surfactant Twin 80 to 200g of the solid medium prepared by the method of the above (b) and pulverizing.

Sample (6) d) A solid medium prepared by the method (b) above, kneaded in a mixer, semi-dried, and then ground.

(E) Unprocessed product (semi-raw product) in which the solid medium prepared by the above method (b) is not crushed and dried. Control

Each of these samples (1) to (6) and the control was treated in a plastic container, and 6 chafers were left in each, and the death situation was observed for 15 days. The results are shown in Table 1.

[0028]

[Table 1]

Note 1) Each sample was sprayed, and 6 days of each of the 6 kinds of scarab beetles were allowed to stand after 2 days, and the total number of deaths after 15 days was displayed as a ratio. Note 2) Six beetles were left for each, and the number of worms that died due to mold on the body in 15 days was expressed as a ratio to the first 6 worms. Note 3) Number of days until half of the 6 scarab beetles die. Note 4) The number of days until half of the 6 scarab beetles are left on mold and die. Note 5) The solid medium mainly composed of branula to which the metalridium anisoprie bacterium obtained in the above step b) was adhered by culture was treated. Note 6) Metallicdium anisopree bacterium was not added at all.

As is clear from Table 1, the samples (1) to (5) to which liquid paraffin and vegetable oil (salad oil) were added and pulverized had the same values as those of the control, which were 1 by 15 days.
00% of the insects died, showing a good insecticidal effect.

In particular, a sample to which vegetable oil (salad oil) was added
(3) and (4) showed extremely good insecticidal effect, which was almost the same as the control. The sample (6) without addition of the water-insoluble sticky substance and crushed in a semi-dried state is slightly inferior to the control in terms of the effect, but the insecticidal effect is retained even if it is in the form of granules of 3 mm or less. It is presumed that the decrease in the effect was due to the scattering of spores and the death of mycelium during crushing and drying.

[0032]

INDUSTRIAL APPLICABILITY As a method for controlling scarab beetles in a vast area such as lawn by microorganisms by the method of the present invention, it has the following advantages as an alternative to chemical pesticides.

(1) Targeting the control of larvae of scarab beetles inhabiting soil, by selecting a bacterium having a particularly large effect,
It is more effective than conventional methods for controlling fruit trees and pests in preventing damage to the roots of lawns and field crops.

(2) Since the particles are small, they can be sprayed near the grass roots, have good spraying efficiency, and have a great control effect.

(3) Since the particle size is small and the sticky substance is added, scattering and runoff due to wind and rainwater is small, and the control effect is highly likely to last for a long time.

(4) A large amount can be expected to be sprayed inexpensively by a mechanical method and a great effect can be expected. In addition, it can be sprayed evenly over a wide area and work efficiency is good.

(5) When pulverizing a solid medium mainly composed of bran cells to which bacteria are adhered, it is possible to make the pulverization work very easy to add an adhesive substance and prevent the dispersal of bacteria and spores better than expected. did it.

 ─────────────────────────────────────────────────── ─── Continued Front Page (72) Inventor Masaaki Sawada 7-28-13 Isobe, Chiba City, Chiba Prefecture (72) Inventor Makoto Hasegawa 2-21-4 Senjo Taipei, Chiba City, Chiba Prefecture (72) Inventor Masafumi Amimura 378 Nakaguro, Iwade-cho, Naga-gun, Wakayama (72) Inventor Masaaki Kai 1877-260, Noda, Kumatori-cho, Sennan-gun, Osaka Prefecture (72) Inventor, Chidan Mizoguchi 9-415 Daito-cho, Ashiya-shi, Hyogo (72) Invention Ichiro Miura 2-chome, Shinsenrikitamachi, Toyonaka City, Osaka Prefecture C62-207 (56) Reference JP-A-2-88510 (JP, A) JP-A-63-148981 (JP, A) JP-A-63-190807 ( JP, A)

Claims (2)

[Claims] 1. Hus consisting of granulated bran pellets, to which metalridium anisoprie bacterium has been adhered by culture.
Liquid paraffin, oils, etc.
Add insoluble sticky material and grind it to a particle size of 3 mm or less.
A method for controlling pests, which comprises spraying granular granular bran powder. 2. A metarhizium anisopree No. 31 strain (Metarhizium a
nisopliae No. 31; Microbiology Research Institute No. 12729).