JPH07324012A - Disinfectant composition and disinfecting method using the same - Google Patents

Abstract

PURPOSE:To obtain a disinfectant composition suitably useful for preventing so-called hospital-acquired infection, having excellent durability, water resistance and washing resistance in disinfecting effect and to provide a disinfecting method using the disinfectant composition. CONSTITUTION:This disinfectant composition is obtained by dispersing a water- insoluble silver-based inorganic germicide or it and chlorhexidine hydrochloride into an alcoholic solution of a hydrophilic polymer capable of forming a water- insoluble film having 30-50% water content. A target material to be disinfected is treated with a the composition to form a coating film containing the water- insoluble silver-based inorganic germicide or the silver-based inorganic germicide and chlorhexidine hydrochloride immobilized by a hydrophilic polymer.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a disinfectant composition which can be suitably used in various fields such as medical care, food, research and the like, and a disinfection method using the same, and in particular, has excellent durability and water resistance in disinfection effect. TECHNICAL FIELD The present invention relates to a disinfectant composition having antibacterial properties and washing resistance, and a disinfection method using the same.

[0002]

BACKGROUND ART In recent years, in hospitals and the like, in order to prevent so-called nosocomial infection, disinfection of in-hospital equipment and its environment has been carefully performed. And usually this disinfection
Aqueous solutions or alcohol solutions of various germicides are used. However, since the disinfection method using such a disinfectant lacks the sustainability, it is necessary to frequently perform the disinfection process if it is desired to obtain the disinfection effect for a long period of time. So it took a lot of effort. Also, when disinfecting textile products such as gowns and curtains, the disinfecting effect did not last until after washing, because the germicide was washed off when the textile products were washed.

Therefore, as one of the attempts to improve the sustainability of such a disinfecting effect, Japanese Patent Application Laid-Open No. 61-233.
No. 602 discloses a liquid composition prepared by dissolving a mixture of a film-forming water-insoluble copolymer and a bactericide in a volatile solvent such as alcohol. Since this liquid composition contains a film-forming polymer, a disinfecting treatment with the composition causes a film containing a bactericide to be formed on the surface of the object to be disinfected so as to exert a bactericidal effect. In addition, the elution of the bactericidal agent contained in this film is prevented by suppressing the swelling of the polymer, thereby improving the sustainability of the bactericidal effect. However, even in such a composition, since a water-soluble bactericidal agent such as a phenolic bactericidal agent or a quaternary ammonium salt is used as a bactericidal agent, for example, a textile product disinfected with this composition is washed. In this case, the disinfection effect is difficult to maintain for a long time.

On the other hand, a method of manufacturing an antibacterial fiber by kneading a silver-based inorganic bactericidal agent into a synthetic fiber to obtain an antibacterial gown or curtain has been studied. In the case of antibacterial products, the durability of antibacterial effect against washing is excellent, but the bactericidal effect is not sufficient, and only the growth of bacteria is suppressed. This is because the silver-based inorganic bactericidal agent is dispersed throughout the fiber and only slightly appears on the surface of the fiber, so that a strong bactericidal property cannot be exhibited.

Further, silver-based fungicides are generally E. coli,
Although effective against Gram-negative bacteria such as Pseudomonas aeruginosa, some are not effective against Gram-positive bacteria such as Micrococcus (Micorococcus). When such bacteria are present, a silver-based fungicide alone is used. There is also an inherent problem that a sufficient bactericidal effect cannot be obtained only by using it.

[0006]

The present invention has been made in view of such circumstances, and the problem to be solved is that the disinfection effect, that is, the sterilizing effect is excellent in durability, water resistance, and washing resistance. Disclosed is a disinfectant composition having the above and a disinfection method using the same, and in addition, a disinfectant composition having a broad antibacterial spectrum and a disinfection method using the same. .

[0007]

In order to solve the above problems, in the present invention, an alcoholic solution of a hydrophilic polymer having the ability to form a water-insoluble film having a water content of 30 to 50% is used. The gist is a disinfectant composition characterized in that a water-insoluble silver-based inorganic bactericide is dispersed therein.

Further, according to the present invention, the water content is 30 to 30.
A disinfectant composition comprising a water-insoluble silver-based inorganic bactericidal agent and chlorhexidine hydrochloride dispersed in an alcoholic solution of a hydrophilic polymer having the ability to form a 50% water-insoluble film. It is a summary.

The present invention further provides that the water-insoluble silver fixed to the surface of the disinfecting target by the hydrophilic polymer by treating the predetermined disinfecting target with the disinfectant composition. A gist of a disinfecting method is characterized by forming a film containing a chlorinated inorganic bactericide or the chlorhexidine hydrochloride.

[0010]

[Operation / Effect] In short, in the disinfectant composition according to the present invention, the silver-based inorganic bactericide is dispersed in an alcoholic solvent solution of a hydrophilic polymer (hereinafter referred to as hydrophilic polymer) having the ability to form a water-insoluble film. Therefore, when such a composition is applied in the disinfection treatment, a polymer film containing a bactericide component is formed on the surface of the object to be disinfected, so that the bactericidal action is effectively exerted. In addition to being able to be performed, a continuous bactericidal effect (disinfecting effect) can be obtained.

More specifically, since the disinfectant composition of the present invention uses an alcoholic solvent such as ethanol for disinfection as a solvent, when the disinfectant composition is applied, first, The strong bactericidal effect of the alcoholic solvent is exhibited. Then, when the alcoholic solvent evaporates, a water-insoluble polymer coating containing a bactericide (hereinafter referred to as polymer coating) is formed on the surface to be disinfected by the hydrophilic polymer. The hydrophilic polymer forming the polymer film is selected such that the water content of the polymer film formed by the hydrophilic polymer is high and the water content is 30 to 50%. Therefore, the polymer coating contains an appropriate amount of water, and thus the bactericidal effect of the bactericidal component dispersed in the coating is advantageously exhibited.

Since the silver-based bactericide is insoluble in water or only slightly dissolved in water, these bactericide components do not dissolve even when the polymer coating comes into contact with water, and , Even if it is a polymer coating, it is insoluble in water,
Moisture content is not too high. That is, since the disinfectant composition according to the present invention does not flow out the bactericide which is an active ingredient of the disinfectant effect even when it is brought into contact with water, in the disinfectant effect, excellent water resistance and wash resistance can be obtained. Therefore, the disinfection effect, that is, the sustainability of the bactericidal effect is advantageously extended.

Further, in the present invention, chlorhexidine hydrochloride is advantageously used in combination with the silver-based inorganic bactericide, in which case the bactericides have a synergistic effect,
Therefore, it has a broad antibacterial spectrum that is effective against Gram-negative bacteria, Gram-positive bacteria and the like. Since such chlorhexidine hydrochloride is almost insoluble in water, it has no adverse effect on water resistance and washing resistance.

As described above, the disinfectant composition according to the present invention is a textile product such as a gown, a curtain, and bed sheets, footwear such as slippers and shoes, beds, and various equipment used in hospitals and the like. It can be suitably used for disinfecting hospital environments such as walls, pillars, elevators, and toilets, and it can solve the problems that conventional disinfectants have, and is excellent in its disinfecting effect. Therefore, it is possible to exhibit the water resistance, the water resistance, and the washing resistance.

Further, according to the disinfection method of the present invention, the object to be disinfected is treated by spraying or applying the above-mentioned disinfectant composition. After the strong bactericidal effect is exhibited, and then the alcoholic solvent is evaporated, the water-insoluble polymer film containing the bactericidal component formed on the surface of the disinfection target exhibits an excellent bactericidal effect. Of. And, the polymer film formed here, even if it is watered or washed, since it is difficult to peel off, the bactericidal effect is maintained for a long period of time, therefore, the disinfection method according to the present invention. According to the method, the troublesomeness of the conventional disinfection method of performing the disinfection treatment many times can be advantageously eliminated.

[0016]

SPECIFIC STRUCTURE By the way, as the hydrophilic polymer constituting the disinfectant composition according to the present invention, 30 to 50 is used.
% Is a hydrophilic linear polymer having the ability to form a water-insoluble film, and is applicable to alcoholic solvents such as methanol, ethanol, or those obtained by adding isopropanol or water, particularly preferably disinfecting ethanol. A melt is used. Specifically, a linear homopolymer of 2-hydroxyethyl methacrylate or 2-
A copolymer or the like of hydroxyethyl methacrylate and another hydrophilic and / or hydrophobic monomer will be used. In addition, as the hydrophilic copolymerizable monomer here,
Acrylamide, N, N'-dimethylacrylamide,
Methacrylic acid, acrylic acid, etc. can be mentioned.
Examples of the hydrophobic copolymerization monomer include alkyl methacrylate, alkyl acrylate, styrene and the like. In addition, the hydrophilic polymer may use vinyl acetate as a copolymerization monomer, and further, a copolymer with a hydrophilic monomer other than 2-hydroxyethyl methacrylate, as long as the above conditions are satisfied, Is also adopted.

The water content of the polymer coating formed by such a hydrophilic polymer must be within the range of 30 to 50%, preferably 33 to 40, in the state where the disinfectant component is not contained. %. If the water content is less than 30%, the bactericidal effect cannot be sufficiently exerted, and if the water content exceeds 50%, the swelling of the coating becomes excessive and the water resistance and This is because the washability becomes insufficient.

The following method is used to measure the water content. That is, a methanol solution containing 3% by weight of a polymer was prepared and poured into a petri dish,
Evaporate the solvent to form a film. And
This film was immersed in distilled water at 20 ° C. for 12 hours or more, then taken out with tweezers, the surface water was quickly wiped off with a filter paper, and then weighed. The weight at this time was taken as the wet weight. The film in the water-containing state was dried at 60 ° C. for 12 hours or more using a dryer, and then weighed, and the weight at this time was defined as the dry weight. Then, the water content is calculated by the following formula. Moisture content (%) = {(wet weight-dry weight) / wet weight} × 100

By the way, as the hydrophilic polymer used in the present invention, a linear homopolymer of 2-hydroxyethyl methacrylate is particularly preferable, and its water content is in the range of 30 to 40%. When such a homopolymer is used, the bactericidal effect is high, the washing resistance is excellent, and the film formed on the surface to be sterilized gives a dry feel. Furthermore, when it is applied to textile products, it becomes soft when exposed to moisture, and therefore it is extremely suitable for disinfecting sheets, gowns and the like. The particularly preferable water content in the case of the copolymer is 33 to 40%.

On the other hand, as the disinfectant constituting the disinfectant composition according to the present invention, a silver-based inorganic disinfectant, that is, an inorganic disinfectant carrying silver is used. It should be noted that the silver-based inorganic bactericidal agent referred to here includes silver, or those in which copper, zinc or the like is supported together with silver. Specifically, as a silver-based inorganic germicide,
Zeolite carrying silver ions by ion exchange (manufactured by Shinagawa Fuel Co., Ltd.), calcium phosphate adsorbing silver (manufactured by Sangi Co., Ltd.), zirconium phosphate carrying silver ions by ion exchange ( Toa Gosei Co., Ltd., a silicon oxide (SiO ₂ ) with a silver complex adsorbed (Matsushita Electric Co., Ltd.), a glass component in which silver oxide is dissolved (Ishizuka Glass Co., Ltd.), and a titania carrier with silver ions. And the like can be mentioned.

Furthermore, Staphylococcus (Staphylococcus), which is not effective only by the above-mentioned silver-based inorganic bactericide, can be obtained.
For the sterilization of many gram-positive bacteria such as genus (cus), chlorhexidine hydrochloride is used together. By the way, since chlorhexidine hydrochloride is insoluble in water, even if it is added, it has no adverse effect on water resistance and washing resistance.
And by the addition of such chlorhexidine hydrochloride,
A synergistic effect with the silver-based inorganic bactericidal agent is caused, so that it has a broad antibacterial spectrum effective against Gram-negative bacteria, Gram-positive bacteria and the like.

As a solvent for dissolving the water-soluble polymer, an alcoholic solvent such as ethanol, methanol, or those obtained by adding isopropanol or water to them is used. From the viewpoint of bactericidal effect, ethanol is preferably used, and more preferably, disinfecting ethanol having an ethanol concentration of 70 to 85% by weight is advantageously used. In addition, any of these alcoholic solvents to which a solvent other than alcohol such as dimethylformamide or dimethylsulfoxide is added may be used.

The hydrophilic polymer is dissolved in the alcoholic solvent, and the disinfectant is mixed and dispersed to prepare the disinfectant composition according to the present invention. Of course, a hydrophilic polymer and a bactericidal agent are simultaneously added to an alcoholic solvent and dissolved or dispersed therein, or a bactericidal agent and an alcohol are added to a hydrophilic polymer solution polymerized in the solvent to obtain a desired disinfectant. It goes without saying that it does not matter if the composition is prepared.

In the disinfectant composition according to the present invention prepared as described above, the polymer concentration in the composition is usually 0.05 to 5% by weight, preferably 0.2 to 3%. Weight% This is because when the polymer concentration is less than 0.05% by weight, the fixing effect of the bactericide becomes insufficient, while when it exceeds 5% by weight, the viscosity of the disinfectant composition increases. This is because it becomes difficult to disinfect. The concentration of the bactericide contained in the disinfectant composition according to the present invention is 0.01 to 5% by weight, preferably 0.05 to 3% by weight. Further, the weight ratio of the hydrophilic polymer to the bactericide contained in the disinfectant composition is about 100: 1 to 1: 2, preferably 100: 3 to 1: 1. Kashi,
If the proportion of the bactericide is too low, the bactericidal effect cannot be sufficiently obtained, and if the ratio is too high, the bactericide on the sterilized surface becomes excessive and the appearance of the treated surface is impaired, or the bactericide adheres. This is because there will be problems such as insufficient.

[0025]

EXAMPLES In order to clarify the present invention in more detail, some examples of the present invention will be shown below.
It goes without saying that the present invention is not limited by the description of such embodiments. In addition to the following embodiments, the present invention further includes various changes and modifications based on the knowledge of those skilled in the art, in addition to the above specific description, without departing from the spirit of the present invention. It should be understood that improvements and the like can be added.

Example 1 First, a stirrer and a Dimroth were set in a 500 mL three-necked round-bottomed flask and the inside was thoroughly replaced with nitrogen, and then 2-hydroxyethyl methacrylate (Wako Pure Chemical Industries, Ltd. (Manufactured by the company): 50.01 g, methanol (manufactured by Wako Pure Chemical Industries, Ltd.): 200 mL, and 2,
0.63 g of 2′-azobis (isobutyronitrile) was inserted, and polymerization was carried out at 60 ° C. for 3 hours while stirring. Then, the obtained polymerization reaction product solution was cooled and then further diluted with 100 mL of methanol.
Then, the methanol solution of the linear 2-hydroxyethyl methacrylate polymer thus obtained was gradually added to 10 L of ethyl acetate (manufactured by Wako Pure Chemical Industries, Ltd.) while the linear 2-hydroxyethyl methacrylate was added. The methacrylate polymer was reprecipitated. Then, after filtering such a precipitate, it is dried under reduced pressure, and 35.5 g of the linear 2-
A hydroxyethyl methacrylate polymer was obtained. The water content of the polymer thus obtained was measured and found to be 38%.

Next, 0.6 g of the obtained 2-hydroxyethyl methacrylate polymer was dissolved in 24 g of 80% by weight concentration of ethanol for disinfection in the Japanese Pharmacopoeia (manufactured by Kanka Chemical Industry Co., Ltd.): Antibacterial zeolite (Zeomic: manufactured by Shinagawa Fuel Co., Ltd.): mixed with 0.12 g,
After dispersal, the intended disinfectant composition was prepared. Then, this disinfectant composition solution was applied to both surfaces of the slide glass and dried. The weight increase of the slide glass after air drying was 0.03 g. Next, this slide glass was immersed in tap water for 48 hours and then air-dried again.

Then, add 15 to a petri dish (diameter 90 mm).
A vinyl chloride wrap cut into 0 mm × 150 mm is laid, the slide glass is placed on the wrap, and Staphylococcus aureus IFO 13276 bacterial solution: 0.2
Loaded mL. Then, the slide glass was wrapped with the wrap so that the bacterial solution uniformly contacted the slide glass, and then the lid of the petri dish was closed. After this was left at 23 ° C. for 6 hours, the slide glass and the wrap were washed away with 4.8 mL of physiological saline, and the slide glass and the wrap were cultured in a tryptosoy agar medium, and the viable cell count per slide glass was measured. The results are shown in Table 1.

Comparative Example 1 In place of the slide glass treated with the disinfectant in Example 1, the slide glass not subjected to the sterilization treatment was subjected to the bacterial solution treatment, and thereafter, in the same manner as in Example 1,
The growth state of the fungus was examined, and the results are also shown in Table 1.

Comparative Example 2 Linear 2-hydroxyethyl methacrylate polymer:
0.6 g was dissolved in 24 g of ethanol for disinfection of Japanese Pharmacopoeia at a concentration of 80% by weight. Then, the obtained solution was applied to a slide glass in the same manner as in Example 1, and the growth state of the bacteria was examined in the same manner as in Example 1, and the results are shown in Table 1.

Examples 2 and 3 Ion Pure Type A (manufactured by Ishizuka Glass Co., Ltd.) or silver-supported calcium phosphate (Novalon: manufactured by Toagosei Co., Ltd.) instead of the antibacterial zeolite (Zeomic).
The intended disinfectant composition was prepared in the same manner as in Example 1 except that each of the above was used as the disinfectant composition of Examples 2 and 3. Further, using them, the slide glass was sterilized, and the bacterial solution treatment was performed under the same conditions as in Example 1 to examine the growth state of the bacteria. The results are shown in Table 1.

[0032]

[Table 1]

Example 4 Linear 2-hydroxyethyl methacrylate polymer:
The same disinfectant composition as in Example 1 was prepared by dissolving 0.4 g of ethanol for disinfection in Japan Pharmacopoeia at a concentration of 80% by weight: 16 g, and mixing and dispersing antibacterial zeolite (Zeomic): 0.08 g in the ethanol. Was prepared, and the slide glass was sterilized using this. Then, on this slide glass, as a bacterium, a bacterial solution treatment was carried out under the same conditions as in Example 1 except that Seratia spp and Micrococcus spp isolated from the preservation solution of a patient using a contact lens were used. Then, the growth state of the bacteria was examined. The results are also shown in Tables 2 and 3.

Examples 5 to 7 Instead of the antibacterial zeolite in Example 4, ion pure type A: 0.08 g, antibacterial zeolite:
0.04 g and chlorhexidine hydrochloride (manufactured by Tokyo Chemical Industry Co., Ltd.): 0.04 g, Ion Pure Type A: 0.0
A disinfectant composition was prepared using 4 g and chlorhexidine hydrochloride: 0.04 g, and used as the disinfectant compositions of Examples 5, 6, and 7. Then, the slide glass was sterilized using these compositions, and then treated with a bacterial solution under the same conditions as in Example 4, and then the growth state of the bacteria was examined.
The results are shown in Tables 2 and 3.

Comparative Examples 3 and 4 A composition containing 0.08 g of antibacterial zeolite instead of the antibacterial zeolite of Example 4 (Comparative Example 3),
Compositions containing nothing (Comparative Example 4) were prepared, and slide glasses were sterilized using these compositions. And
The slide glass was treated with the bacterial solution in the same manner as in Example 4, and the growth state of the bacteria was examined. The results are shown in Table 2.
And shown in Table 3.

[0036]

[Table 2]

[0037]

[Table 3]

Example 8 and Comparative Example 5 A spray container was filled with the disinfectant composition prepared in the same manner as in Example 7, and a cotton broad fabric was spray-treated and a cotton broad fabric that had not been treated at all. , Prepared respectively. Then, weave those fabrics in about 1 cm x 1 c
It was cut so as to have a length of m, and about 0.75 g of the sample was collected to obtain a disinfecting treated fabric sample and an untreated fabric sample. In addition, 70 mL of phosphate buffer solution was added to a 200 mL Erlenmeyer flask with a screw cap to sterilize it.
Next, Staphylococcus aureus bacterial suspension (5.6 × 10 ⁴ cells / mL): 5 mL was added and mixed to prepare 6 mixtures. Furthermore, the said textile sample was added to each of them, and the sample was prepared by shaking with a shaker for 1 hour. In addition, three specimens were used for each of the specimens using the disinfected sample (Example 8) and the specimen using the untreated sample (Comparative Example 5). After such shaking operation,
The number of viable bacteria in each bacterial turbid solution of the sample was measured by the plate pour method, and the average viable cell count of each of the sterilized sample and the untreated sample is shown in Table 4.

Example 9 Three samples of the disinfected fabric sample obtained in Example 8 were prepared, and a three-step operation of washing them with a neutral detergent, rinsing, and drying was performed on them. 5 each
Repeated times. Then, using the sample after the washing, a microbial test was performed using Staphylococcus aureus in the same manner as in Example 8, and the results are also shown in Table 4.

[0040]

[Table 4]

Claims (3)

[Claims] 1. A disinfection characterized in that a water-insoluble silver-based inorganic bactericide is dispersed in an alcoholic solution of a hydrophilic polymer having a water content of 30 to 50% and capable of forming a water-insoluble film. Agent composition. 2. A water-insoluble silver-based inorganic bactericide and chlorhexidine hydrochloride dispersed in an alcoholic solution of a hydrophilic polymer having a water content of 30 to 50% to form a water-insoluble film. Disinfectant composition. 3. The water-insoluble, fixed to the surface of the disinfecting target by the hydrophilic polymer by treating the disinfecting target with the disinfectant composition according to claim 1 or 2. A disinfection method comprising forming a silver-based inorganic bactericidal agent or a film containing the silver-based inorganic bactericidal agent and chlorhexidine hydrochloride.