JPH07218469A - Hydrogen gas measurement method - Google Patents
Hydrogen gas measurement methodInfo
- Publication number
- JPH07218469A JPH07218469A JP6027607A JP2760794A JPH07218469A JP H07218469 A JPH07218469 A JP H07218469A JP 6027607 A JP6027607 A JP 6027607A JP 2760794 A JP2760794 A JP 2760794A JP H07218469 A JPH07218469 A JP H07218469A
- Authority
- JP
- Japan
- Prior art keywords
- hydrogen
- fuel cell
- bacterium
- hydrogen gas
- gas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 title claims abstract description 61
- 238000000691 measurement method Methods 0.000 title claims description 3
- 239000001257 hydrogen Substances 0.000 claims abstract description 48
- 229910052739 hydrogen Inorganic materials 0.000 claims abstract description 48
- 241000894006 Bacteria Species 0.000 claims abstract description 23
- 239000000446 fuel Substances 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 18
- 244000005700 microbiome Species 0.000 claims abstract description 13
- 238000004519 manufacturing process Methods 0.000 claims description 24
- 238000005259 measurement Methods 0.000 claims description 8
- 239000001301 oxygen Substances 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 241000193403 Clostridium Species 0.000 claims description 4
- 230000000243 photosynthetic effect Effects 0.000 claims description 4
- 241000190932 Rhodopseudomonas Species 0.000 claims description 2
- 241001148471 unidentified anaerobic bacterium Species 0.000 claims description 2
- 101100412856 Mus musculus Rhod gene Proteins 0.000 claims 1
- 241000190967 Rhodospirillum Species 0.000 claims 1
- 101100242191 Tetraodon nigroviridis rho gene Proteins 0.000 claims 1
- 238000012216 screening Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 239000007789 gas Substances 0.000 description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 150000002431 hydrogen Chemical class 0.000 description 4
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 238000004817 gas chromatography Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 229910052697 platinum Inorganic materials 0.000 description 2
- 241000193171 Clostridium butyricum Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000190984 Rhodospirillum rubrum Species 0.000 description 1
- 241001441724 Tetraodontidae Species 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 235000014121 butter Nutrition 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000011549 displacement method Methods 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000003014 ion exchange membrane Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 102220201851 rs143406017 Human genes 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
(57)【要約】
【構成】 微生物によって生産された水素ガスを、燃料
電池を利用して測定することを特徴とする水素ガスの計
測法。
【効果】 高価な装置を用いることなく、水素ガスを迅
速且つ簡便にしかも正確に計測することができるのみで
なく、本法を利用することにより水素産生菌のスクリー
ニングも可能である。(57) [Summary] [Structure] A hydrogen gas measuring method characterized in that hydrogen gas produced by microorganisms is measured using a fuel cell. [Effect] Not only can hydrogen gas be measured quickly, simply and accurately without using an expensive device, but also by using this method, screening of hydrogen-producing bacteria is possible.
Description
【０００１】[0001]
【産業上の利用分野】本発明は、水素の計測法に関し、
更に詳細には微生物による水素生産における水素生産速
度及び水素生産量の計測に関するものである。また、本
発明は、水素計測法を利用した水素産生菌のスクリーニ
ング法に関するものである。FIELD OF THE INVENTION The present invention relates to a method for measuring hydrogen,
More specifically, it relates to measurement of hydrogen production rate and hydrogen production amount in hydrogen production by microorganisms. The present invention also relates to a screening method for hydrogen-producing bacteria using a hydrogen measurement method.
【０００２】[0002]
【従来の技術】従来、ガスの瞬間流量（速度）、積算流
量の測定にはガスメーター等の計量法に準じた機器が使
用されている。ガスメーターには湿式ガスメーターと乾
式ガスメーターとがあり、測定ガスによって使い分けら
れている。しかし、湿式では水蒸気圧の影響やガスの可
溶化、乾式では水分を含むガスの測定が困難であったり
する。また、ガスメーターによる測定では測定ガスの流
量がある一定以上の大きさが必要であり、微小流量のも
のに関しては大きな誤差を生じる。2. Description of the Related Art Conventionally, an apparatus conforming to a measuring method such as a gas meter has been used for measuring an instantaneous flow rate (speed) of gas and an integrated flow rate. Gas meters include a wet gas meter and a dry gas meter, which are used properly depending on the measurement gas. However, the influence of water vapor pressure and solubilization of gas in the wet method and the measurement of gas containing water in the dry method are difficult. Further, in the measurement by the gas meter, the flow rate of the measurement gas needs to be a certain value or more, and a large error occurs when the flow rate is very small.
【０００３】一方、微生物を利用した水素生産法では、
生産ガスの速度、生産量は微生物の活性に支配され、そ
の量は微小で一定でなく、測定が困難である。また、水
素以外に炭酸ガスやメタンなどのガス生成があり、水素
のみの生産速度や流量を測定するためには、ガスクロマ
トグラフィーを用いてガスの組成分析を更に行う必要が
ある。On the other hand, in the hydrogen production method using microorganisms,
The rate of production gas and the amount of production are controlled by the activity of microorganisms, and the amount is minute and not constant, making it difficult to measure. In addition to hydrogen, carbon dioxide, methane, and other gases are also generated, and in order to measure the production rate and flow rate of only hydrogen, it is necessary to further analyze the composition of the gas using gas chromatography.
【０００４】[0004]
【発明が解決しようとする課題】本発明は、このような
技術の現状のもとでなされたものであって、本発明の目
的は、微生物生産法によって生産された水素を、迅速か
つ簡便にしかも正確に測定できる新規な計測法を提供す
るにある。The present invention has been made under the present circumstances of such a technology, and an object of the present invention is to rapidly and simply produce hydrogen produced by a microbial production method. Moreover, it is to provide a new measuring method that can accurately measure.
【０００５】[0005]
【課題を解決するための手段】本発明は、上記目的を達
成するためになされたものであって、各方面から検討し
た結果、燃料電池にはじめて着目した。すなわち、本発
明者らは、燃料電池の中で特に、水素−酸素型燃料電池
が、次式（下記化１）で水素の酸化反応過程における自
由エネルギー変化を直接電流に変換することに注目し
た。Means for Solving the Problems The present invention has been made in order to achieve the above-mentioned object, and as a result of study from various aspects, the first attention was paid to the fuel cell. That is, the present inventors have paid attention to the fact that the hydrogen-oxygen fuel cell, among other fuel cells, converts the free energy change in the hydrogen oxidation reaction process directly into a current according to the following formula (Formula 1 below). .
【０００６】[0006]
【化１】 [Chemical 1]
【０００７】上式より、分極がない（電流を取り出さな
い）ときの端子電圧Ｅ0は１．２３Ｖになる。そこで、
一定の抵抗を与えることで微生物が生産する水素量に従
って電流値が変化することに注目した。From the above equation, the terminal voltage E 0 when there is no polarization (no current is taken out) is 1.23V. Therefore,
We paid attention to the fact that the current value changes according to the amount of hydrogen produced by microorganisms by giving a constant resistance.
【０００８】本発明は、これらの点にはじめて注目し、
そして更に研究、検討を重ねた結果、遂に完成されたも
のであって、微生物を利用した水素生産リアクターから
生成するガスを、水素−酸素型燃料電池と組み合わせる
ことで、簡便かつ迅速に水素生産速度および水素生産量
を電流値の変化から計測するものである。The present invention focuses on these points for the first time,
As a result of further research and study, it was finally completed.By combining the gas generated from the hydrogen production reactor utilizing microorganisms with the hydrogen-oxygen fuel cell, the hydrogen production rate can be easily and rapidly increased. And the amount of hydrogen production is measured from the change in current value.
【０００９】本発明によれば、微量しか生成されずしか
も生成量が一定しない微生物由来の水素ガスをきわめて
効率よく計測することができ、すべてのタイプの水素産
生微生物を産生する水素ガスを計測することができる。According to the present invention, it is possible to extremely efficiently measure hydrogen gas derived from a microorganism that produces only a trace amount and does not produce a constant amount, and to measure hydrogen gas that produces all types of hydrogen-producing microorganisms. be able to.
【００１０】水素産生微生物としては、嫌気性菌、光合
成細菌その他水素産生菌であればすべてのタイプのもの
が挙げられる。例えば嫌気性菌としては、クロストリジ
ウム（Ｃｌｏｓｔｒｉｄｉｕｍ）属菌、光合成細菌とし
ては、ロドスピリウム（Ｒｈｏｄｏｓｐｉｒｉｌｌｕ
ｍ）属菌、ロドシュードモナス（Ｒｈｏｄｏｐｓｅｕｄ
ｏｍｏｎａｓ）属菌等が挙げられる。The hydrogen-producing microorganisms include all types of anaerobic bacteria, photosynthetic bacteria and other hydrogen-producing bacteria. For example, an anaerobic bacterium is a genus Clostridium, and a photosynthetic bacterium is Rhodospirillu.
m) genus, Rhodopseud
Omonas) and the like.
【００１１】更に具体的には次のものが例示される。Ｃ
ｌｏｓｔｒｉｄｉｕｍ ｂｕｔｙｒｉｃｕｍ、Ｃ．ａｃ
ｅｔｏｂｕｔｙｌｉｃｕｍ等Ｃｌｏｓｔｒｉｄｉｕｍ属
菌；Ｒｈｏｄｏｓｐｉｒｉｌｌｕｍ ｒｕｂｒｕｍ等Ｒ
ｈｏｄｏｓｐｉｒｉｌｌｕｍ属菌；Ｒｈｏｄｏｐｓｅｕ
ｄｏｍｏｎａｓ ｓｐｈｅｒｏｉｄｅｓ等Ｒｈｏｄｏｐ
ｓｅｕｄｏｍｏｎａｓ属菌その他。More specifically, the following are exemplified. C
lostridium butyricum, C.I. ac
Clostridium spp. such as Etobutylicum; Rhodospirillum rubrum etc. R
Rhodopseu;
domonas spheroides, etc. Rhodop
Other bacterium of the genus seudomonas.
【００１２】また、本発明は、上記したように各種微生
物が生産する水素ガスをきわめて簡便、効率的に、定量
／定性分析することができる。したがって、本発明によ
れば、微生物の水素生産能を簡便且つ迅速に確認するこ
とができるので、水素産生菌のスクリーニングも簡便に
行うことができ、したがって本発明は、水素産生菌のス
クリーニング方法も提供するものである。よって、本発
明は、水素産生菌の分離、選択のほか、同定に利用でき
るだけでなく、新規水素産生菌を新たに探索するのにも
利用することができるので、本発明を利用することによ
り、従来未知のすぐれた水素産生菌を発見する機会が更
に広がり、エネルギーの面のみならず、微生物学の面等
においても本発明は大いに期待されるものである。In addition, the present invention enables very simple and efficient quantitative / qualitative analysis of hydrogen gas produced by various microorganisms as described above. Therefore, according to the present invention, it is possible to easily and quickly confirm the hydrogen-producing ability of a microorganism, and thus it is possible to easily screen for hydrogen-producing bacteria. Therefore, the present invention also provides a method for screening hydrogen-producing bacteria. It is provided. Therefore, the present invention, in addition to separation and selection of hydrogen-producing bacteria, can be used not only for identification, but also for newly searching for new hydrogen-producing bacteria, by using the present invention, The chances of discovering an excellent hydrogen-producing bacterium that has not been heretofore known have further expanded, and the present invention is highly expected not only in terms of energy but also in terms of microbiology.
【００１３】本発明を実施するには、燃料電池を利用す
る必要があるが、燃料電池としては各種のタイプのもの
が適宜使用され、例えば水素−酸素型燃料電池が有利に
使用できる。水素ガスを測定するには、培養槽、リアク
ター等水素ガス源と燃料電池とを接続し、その電流値を
測定し、組成や水蒸気圧を考慮に入れたスタンダードカ
ーブにより水素生産速度及び／又は水素生産量を測定
し、もって水素ガスアッセイを行えばよい。In order to carry out the present invention, it is necessary to utilize a fuel cell, but various types of fuel cells are appropriately used, and for example, a hydrogen-oxygen type fuel cell can be advantageously used. To measure hydrogen gas, connect a hydrogen gas source such as a culture tank or reactor to a fuel cell, measure the current value, and measure the hydrogen production rate and / or hydrogen with a standard curve that takes into account the composition and water vapor pressure. The hydrogen gas assay may be performed by measuring the production amount.
【００１４】水素ガスの測定を行うためには、例えば図
１に示すような測定システムを利用することができる。
図中（ａ）は燃料電池、（ｂ）は本システムの概要を示
すものである。燃料電池は、水素−酸素型燃料電池であ
って、１は白金電極（アノード）網、２はアノード液出
口、３は白金電極（カソード）、４はイオン交換膜、５
はカソード室の電解液（例えば０．０１Ｍ Ｋ3Ｆｅ
（ＣＮ）6）、６はアノード液入口を、それぞれ示す。In order to measure hydrogen gas, for example, a measuring system as shown in FIG. 1 can be used.
In the figure, (a) shows a fuel cell and (b) shows an outline of this system. The fuel cell is a hydrogen-oxygen fuel cell, 1 is a platinum electrode (anode) network, 2 is an anode liquid outlet, 3 is a platinum electrode (cathode), 4 is an ion exchange membrane, 5
Is an electrolyte in the cathode chamber (eg 0.01 M K 3 Fe
(CN) 6 ) and 6 show the anode liquid inlets, respectively.
【００１５】（ｂ）は、本発明を実施するための測定シ
ステムの１例を図示したものであって、燃料電池７と培
養槽１０とをポンプ８を介して結合している。Ａは電流
計を示し、９はマグネチックスターラーを示している。
図１に示した測定システムにおいては、図示したとお
り、培養液を循環させて燃料電池と接続しているが、他
の態様として、水素生産培養槽から生産ガスのみを捕集
して燃料電池に導入するシステムを採用しても何ら差し
支えない。FIG. 1B shows an example of a measuring system for carrying out the present invention, in which a fuel cell 7 and a culture tank 10 are connected via a pump 8. A indicates an ammeter and 9 indicates a magnetic stirrer.
In the measurement system shown in FIG. 1, as shown in the figure, the culture solution is circulated and connected to the fuel cell. However, as another embodiment, only the produced gas is collected from the hydrogen production culture tank to be used in the fuel cell. It does not matter if you adopt the system to be introduced.
【００１６】以下、本発明の実施例について述べる。Examples of the present invention will be described below.
【００１７】[0017]
【実施例１】図１に示した測定システムを用いて水素の
測定を行った。先ず、培養槽において、水素産生菌とし
てＣｌｏｓｔｒｉｄｉｕｍ ｂｕｔｙｒｉｃｕｍを用い
て、グルコースを栄養源とした１Ｌの培養液で水素生産
を行った。Example 1 Hydrogen was measured using the measuring system shown in FIG. First, in a culture tank, Clostridium butyricum was used as a hydrogen-producing bacterium, and hydrogen was produced in 1 L of a culture solution containing glucose as a nutrient.
【００１８】水上置換法（ＨＣｌ水溶液、ｐＨ＜３）で
測定した生産ガスの測定を行い、組成分析、水蒸気圧等
を考慮に入れ水素生産速度および水素生産量を算出し
た。The production gas measured by the water displacement method (HCl aqueous solution, pH <3) was measured, and the hydrogen production rate and the hydrogen production amount were calculated in consideration of composition analysis, water vapor pressure and the like.
【００１９】経時変化に対する水素生成速度と得られた
電流値のピーク曲線の一致性は非常に良好な結果であっ
た（図２）。The agreement between the hydrogen generation rate and the peak curve of the obtained current value with respect to aging was a very good result (FIG. 2).
【００２０】[0020]
【実施例２】培養液にＣｌｏｓｔｒｉｄｉｕｍ ｂｕｔ
ｙｒｉｃｕｍを加えないで、水素ガスのみを導入して水
素導入速度と電流値の関係を測定した。結果を図３に示
す。Example 2 Clostridium butter was added to the culture solution.
Only the hydrogen gas was introduced without adding yricum, and the relationship between the hydrogen introduction rate and the current value was measured. The results are shown in Fig. 3.
【００２１】水素導入速度の増加に比例して電流値も増
加する傾向を示した。The current value tended to increase in proportion to the increase in the hydrogen introduction rate.
【００２２】この結果から、実施例１でのＣｌｏｓｔｒ
ｉｄｉｕｍ ｂｕｔｙｒｉｃｕｍの水素生成速度と電流
値を換算して得られる水素生成速度は非常に良く一致す
ることが示された。From these results, Clostr in Example 1
It was shown that the hydrogen production rate of the idium butyricum and the hydrogen production rate obtained by converting the current value are in very good agreement.
【００２３】また、２４時間の培養で生産した水素ガス
は、電流値を換算して得た水素生成速度を積算した結
果、２，４５０ｍｌであり、実施例１で得られた結果と
良く一致した。The hydrogen gas produced by 24 hours of culturing was 2,450 ml as a result of integrating the hydrogen generation rate obtained by converting the current value, which was in good agreement with the result obtained in Example 1. .
【００２４】[0024]
【発明の効果】以上、詳細に説明した如く、本発明によ
る水素ガスの計測法は微生物生産法で生産された水素を
燃料電池を利用して迅速かつ簡便に計測するので、次の
効果を奏する。As described above in detail, the method for measuring hydrogen gas according to the present invention has the following effects because hydrogen produced by the microbial production method can be measured quickly and simply by using a fuel cell. .
【００２５】（イ）微生物によって生産された水素の生
産速度および生産量をリアルタイムに迅速かつ簡便に計
測することができる。 （ロ）簡単な水素−酸素型燃料電池を接続するだけでよ
く、ガスメーター、ガスクロマトグラフィーなど高価な
装置を必要とせず経済的である。 （ハ）水素産生能が簡便に確認でき、水素産生菌のスク
リーニングが容易になる。 （ニ）従来未知の新規な水素産生菌の発見が容易とな
り、エネルギー業界においてそしてまた微生物業界にお
いても大きな寄与をなすものである。(A) The production rate and production amount of hydrogen produced by the microorganism can be measured in real time quickly and easily. (B) It suffices to connect a simple hydrogen-oxygen type fuel cell, and it is economical without requiring expensive equipment such as a gas meter and gas chromatography. (C) Hydrogen-producing ability can be easily confirmed, and screening of hydrogen-producing bacteria can be facilitated. (D) It becomes easy to discover a novel hydrogen-producing bacterium, which has been unknown until now, and it makes a great contribution to the energy industry and also to the microbial industry.
【図１】燃料電池及び本発明の測定システムの概念図で
ある。FIG. 1 is a conceptual diagram of a fuel cell and a measurement system of the present invention.
【図２】実施例１の結果を示すグラフである。2 is a graph showing the results of Example 1. FIG.
【図３】実施例２の結果を示すグラフである。FIG. 3 is a graph showing the results of Example 2.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 ＦＩ 技術表示箇所 (Ｃ１２Ｑ 1/06 Ｃ１２Ｒ 1:01） ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 6 Identification number Office reference number FI technical display location (C12Q 1/06 C12R 1:01)
Claims (7)
燃料電池を利用して測定することを特徴とする水素ガス
の計測法。1. A hydrogen gas produced by a microorganism,
A method for measuring hydrogen gas, which is characterized by using a fuel cell for measurement.
／又は水素生産量を電流値の変化から計測し、もって水
素ガスを計測すること、を特徴とする請求項１に記載の
計測法。2. The measuring method according to claim 1, wherein the fuel cell is used to measure the hydrogen production rate and / or the hydrogen production amount from the change in the current value, and thereby the hydrogen gas. .
こと、を特徴とする請求項１又は請求項２に記載の計測
法。3. The measuring method according to claim 1, wherein the fuel cell is a hydrogen-oxygen fuel cell.
載の計測法を利用することを特徴とする水素産生菌の分
離、選択方法。4. A method for separating and selecting a hydrogen-producing bacterium, which comprises utilizing the measurement method according to any one of claims 1 to 3.
細菌であることを特徴とする請求項４に記載の方法。5. The method according to claim 4, wherein the hydrogen-producing bacteria are anaerobic bacteria and / or photosynthetic bacteria.
ｔｒｉｄｉｕｍ）属菌であることを特徴とする請求項５
に記載の方法。6. The anaerobic bacterium is Clostridium (Clos).
A bacterium belonging to the genus tridium).
The method described in.
ｏｓｐｉｒｉｌｌｕｍ）属菌及び／又はロドシュードモ
ナス（Ｒｈｏｄｏｐｓｅｕｄｏｍｏｎａｓ）属菌である
ことを特徴とする請求項５に記載の方法。7. The photosynthetic bacterium is Rhodospirillum (Rhod).
The method according to claim 5, which is a genus bacterium of the genus Ospirillum and / or a bacterium of the genus Rhodopseudomonas.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6027607A JPH07218469A (en) | 1994-02-01 | 1994-02-01 | Hydrogen gas measurement method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6027607A JPH07218469A (en) | 1994-02-01 | 1994-02-01 | Hydrogen gas measurement method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH07218469A true JPH07218469A (en) | 1995-08-18 |
Family
ID=12225621
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6027607A Pending JPH07218469A (en) | 1994-02-01 | 1994-02-01 | Hydrogen gas measurement method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH07218469A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0726357A1 (en) * | 1995-02-07 | 1996-08-14 | Betz Laboratories Inc. | Inhibiting anaerobic production of volatile fatty acids and hydrogen by bacteria |
| US7258938B2 (en) | 2001-03-06 | 2007-08-21 | Sharp Kabushiki Kaisha | Polymer electrolyte fuel cell |
| US7432091B2 (en) | 2003-02-24 | 2008-10-07 | Research Institute Of Innovative Technology For The Earth | Highly efficient hydrogen production method using microorganism |
| US8846358B2 (en) | 2008-05-12 | 2014-09-30 | Sharp Kabushiki Kaisha | Method and device for producing hydrogen |
| JP2017020823A (en) * | 2015-07-08 | 2017-01-26 | 東西化学産業株式会社 | Dissolved hydrogen concentration measurement device and measuring method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6349997A (en) * | 1986-08-20 | 1988-03-02 | 富士通株式会社 | Data transmission method |
| JPH02115760A (en) * | 1988-10-26 | 1990-04-27 | Daihen Corp | Method and instrument for measuring dissolved gaseous hydrogen in insulating oil |
| JPH02307051A (en) * | 1989-05-23 | 1990-12-20 | Daihen Corp | Method and instrument for measuring dissolved gaseous hydrogene in insulating oil |
-
1994
- 1994-02-01 JP JP6027607A patent/JPH07218469A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6349997A (en) * | 1986-08-20 | 1988-03-02 | 富士通株式会社 | Data transmission method |
| JPH02115760A (en) * | 1988-10-26 | 1990-04-27 | Daihen Corp | Method and instrument for measuring dissolved gaseous hydrogen in insulating oil |
| JPH02307051A (en) * | 1989-05-23 | 1990-12-20 | Daihen Corp | Method and instrument for measuring dissolved gaseous hydrogene in insulating oil |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0726357A1 (en) * | 1995-02-07 | 1996-08-14 | Betz Laboratories Inc. | Inhibiting anaerobic production of volatile fatty acids and hydrogen by bacteria |
| US7258938B2 (en) | 2001-03-06 | 2007-08-21 | Sharp Kabushiki Kaisha | Polymer electrolyte fuel cell |
| US7527883B2 (en) | 2001-03-06 | 2009-05-05 | Sharp Kabushiki Kaisha | Polymer electrolyte fuel cell |
| US7432091B2 (en) | 2003-02-24 | 2008-10-07 | Research Institute Of Innovative Technology For The Earth | Highly efficient hydrogen production method using microorganism |
| US8846358B2 (en) | 2008-05-12 | 2014-09-30 | Sharp Kabushiki Kaisha | Method and device for producing hydrogen |
| JP2017020823A (en) * | 2015-07-08 | 2017-01-26 | 東西化学産業株式会社 | Dissolved hydrogen concentration measurement device and measuring method |
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