JPH07196705A - Production of saccharide composed of three or more monosaccharide molecules bonded through glycoside linkage - Google Patents

Abstract

PURPOSE:To provide a method for production of a saccharide, capable of saving thermal energy, and simplifying facilitates required for concentration, having an economical advantage and enabling improvement of the chromatographic separation rate and the reverse osmosis membrane separation rate and prevention of putrefaction of a saccharide solution during the treatment. CONSTITUTION:A mixture solution containing a monosaccharide and/or betaine (the first component), a disaccharide (the second component) and a saccharide (the third component) composed of three or more monosaccharide molecules mutually bonded through a glycoside linkage is used as the raw material solution and the three components are mutually separated according to the chromatographic method under a temperature condition of >=50 deg.C. The resultant solution of the third component isolated by the chromatographic separation method is concentrated through a reverse osmosis membrane equipment under a temperature condition of >=50 deg.C. The resultant concentrated solution is evaporated and concentrated to obtain a concentrated solution having 70wt.% solid content and the obtained concentrated solution is used as a commercial product. A filtered solution separated from the concentrated solution by the reverse osmosis membrane equipment is recycled as an eluting water for chromatographic separation.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention relates to a highly concentrated saccharide in which three or more monosaccharide molecules of interest are glycosidic-bonded from a mixed solution of saccharides obtained by enzymatically decomposing starch. The present invention relates to a method for producing a saccharide that can be obtained, in which three or more monosaccharide molecules are glycosidic-bonded.

[0002]

2. Description of the Related Art Generally, sugars are used as sweeteners. Among them, sugars formed by glycosidic bonds of three or more monosaccharide molecules (for example, trisaccharides, tetrasaccharides, etc.) (hereinafter, sugars of trisaccharides or more) Is being welcomed by consumers as a low-calorie sweetener, and the consumption is increasing year by year.

As a method for obtaining saccharides having three or more sugars,
A method of decomposing starch with the enzyme amylase has been adopted. By this enzymatic decomposition, a three-component mixed solution of saccharides such as monosaccharides, disaccharides and trisaccharides or more can be obtained. Chromatographic separation has been conventionally performed as a separation operation for obtaining the target saccharides of three or more sugars from the three-component mixed solution.

In the case of chromatographic separation, water is generally used as a developing solution, and thus the separated saccharides of trisaccharides and above are diluted with water. Therefore, conventionally, the saccharide solution is supplied to an evaporator to be heated to remove moisture. Was evaporated and concentrated to give the desired concentration of sugar.

[0005]

The trisaccharides or higher saccharides separated by the chromatographic separation are obtained as a dilute solution diluted with water as described above, but the trisaccharides or higher saccharides are originally concentrated in the mixed solution. Is lower than other components, and the category in which saccharides of trisaccharides and higher are separated is a category in which many components are contained, and all components with different migration speeds in the chromatographic separation layer are included. Due to the reason that it is a category that must be taken out, etc., it is usually diluted with water as compared with other sugar categories, so it is usually
The concentration of the solution is as low as about 4-5%. on the other hand,
The concentration required as a product is usually about 70%. Therefore, in order to obtain a product, it is necessary to concentrate it about 14 to 18 times, and conventionally, all of this concentration was carried out by a heating evaporation means. However, the evaporative concentration method involving a phase change of evaporation of water requires a large amount of heat energy,
It has a drawback that the energy consumption is large and the production cost is significantly increased. Moreover, in order to carry out high concentration concentration of about 14 to 18 times, a large-scale evaporator is required, the equipment cost is increased, and the equipment space is required, which is economically extremely disadvantageous.

The present inventors have conducted extensive studies in view of the above points, and as a result, after separating a saccharide solution of trisaccharides or more separated by chromatographic separation into a concentrated liquid and a permeated liquid by a reverse osmosis membrane device and concentrating it, It was found that the concentrated solution can be efficiently concentrated to a high concentration by further concentrating the concentrated solution by evaporative concentration.

On the other hand, however, the handling of the saccharide solution causes a particular problem. The first point is the problem of liquid passage resistance in the chromatographic separation step and the concentration step by the reverse osmosis membrane device. That is, since the saccharide solution has a high viscosity, there is a problem that a pressure loss is large at the time of chromatographic separation and separation by a reverse osmosis membrane, and as a result, a decrease in the separation rate thereof cannot be avoided.

As a second point, during the chromatographic separation step, the saccharide solution as a raw material liquid is diluted with eluent water and its concentration is lowered, so that bacteria are generated depending on the temperature conditions, and as a result, the chromatographic separation step and the reverse osmosis membrane. There is a problem that the saccharide solution will rot during the concentration step.

The inventors of the present invention have conducted further studies to solve the above-mentioned problems, and as a result, by setting the treatment temperature at the time of chromatographic separation and separation by a reverse osmosis membrane to 50 ° C. or higher, the separation speed of them is increased. The present inventors have found that it is possible to improve the above-mentioned properties, and further, to prevent bacteria from spoiling and prevent the sugar solution from spoiling. The present invention has been completed based on these findings.

Thus, the present invention provides a saccharide which is excellent in production efficiency, can reduce the production cost, and can prevent spoilage of the saccharide solution during the production process, in which three or more monosaccharide molecules are glycoside-bonded. The purpose is to provide a method.

[0011]

The present invention relates to (1) a monosaccharide and / or betaine (a first component), a disaccharide (a second component), and a saccharide in which three or more monosaccharide molecules are glycoside-bonded. A step of separating the mixed solution containing the (third component) into three components of the above-mentioned first component, second component and third component by a chromatographic separation device under a temperature condition of 50 ° C. or higher; A step of concentrating the three-component solution under a temperature condition of 50 ° C. or higher by a reverse osmosis membrane device to separate it into a concentrate and a permeate, and a step of refluxing the permeate to an eluting water supply part of the chromatographic separator. A method for producing a saccharide comprising three or more monosaccharide molecules glycoside-bonded, wherein the concentrated liquid is heated to perform a second-stage concentration treatment by evaporative concentration. Sugar and / or betaine (first component), disaccharide (Second component) A mixed solution containing saccharides (third component) formed by glycosidic bonds of three or more monosaccharide molecules is subjected to a chromatographic separation device under a temperature condition of 50 ° C. or more by the above-mentioned first and second components. A step of separating the third component into three components, and the third component solution obtained by the chromatographic separation is concentrated by the first reverse osmosis membrane device under a temperature condition of 50 ° C. or higher to obtain a concentrate and a permeate. A step of separating the permeated liquid into an inorganic salt solution and a permeated liquid by introducing the permeated liquid to a second reverse osmosis membrane device, and a chromatographic separation device for separating the permeated liquid by the second reverse osmosis membrane device. And a step of refluxing the concentrated solution separated by the first reverse osmosis membrane device to perform a second-stage concentration treatment by evaporative concentration. Of the saccharides formed by glycosidic bonds of the above monosaccharide molecules The gist of the production method.

The present invention uses a mixed solution containing a monosaccharide (first component), a disaccharide (second component), and a saccharide (third component) formed by glycosidic bonding of three or more monosaccharide molecules as a raw material liquid. , Separate the above three components.

In the present invention, the first component in the mixed solution is a monosaccharide and / or betaine, and examples of the monosaccharide include glucose and fructose. The second component is a disaccharide, and examples of the disaccharide include maltose and sucrose. In the present invention, both monosaccharides and disaccharides are one kind or a mixture of two or more kinds of sugars belonging to them.

In the present invention, as the third component,
Saccharides (sugars with three or more sugars) formed by glycosidic bonds of three or more monosaccharide molecules are oligosaccharides (oligosaccharides)
Of these, saccharides excluding disaccharides, that is, trisaccharides, tetrasaccharides, pentasaccharides, hexasaccharides and the like are meant. The number of constituent monosaccharide molecules can be arbitrarily selected within a range that can be distinguished from polysaccharides, and decasaccharide is also appropriate.

In the present invention, the above-mentioned trisaccharide and higher saccharides are
It is one or a mixture of two or more of the sugars defined above. Examples thereof include maltotriose and raffinose as trisaccharides, maltotetraose as tetrasaccharides, maltopentaose as pentasaccharides, maltohexaose as hexasaccharides, and maltodecaose as decasaccharides.

As a method for obtaining a mixed solution containing such three components, there are the following methods, for example. (1) In the case of using starch as a starting material, liquefaction is performed using a liquefying enzyme containing α-amylase as a main component, and saccharification is performed using a saccharifying enzyme containing glucoamylase and β-amylase as main components, and enzymatic decomposition is performed. A mixed solution containing three components of glucose (first component), maltose (second component), and a mixture of two or more kinds of saccharides including trisaccharides or more (third component) is obtained. (2) Using beet sugar as a starting material The raw material sugar beet is squeezed, filtered, and the filtrate is heated and concentrated, followed by crystallization. A mixed solution containing three components of betaine (first component), sucrose (second component), and raffinose mixed with inorganic salts (third component) is obtained as a residual liquid (molasses) from which crystals are removed. (3) When cane sugar is used as a starting material A mixture of glucose and fructose (first component) is prepared by treating sugar cane as a starting material in the same manner as in (2) above.
A mixed solution containing three components of sucrose (second component) and a mixture of two or more saccharides of three or more saccharides (third component) in which inorganic salts are mixed is obtained.

The mixed solution obtained as described above is used as a raw material liquid, and this is subjected to three-component separation by a chromatographic method. As a method of separating three components, 1) a method using a pseudo moving bed type multi-component separating apparatus (Japanese Patent Laid-Open No. 4-2278).
04), 2) using a fixed-bed type chromatographic separation device,
A method for continuously fractionating enriched fractions of three or more components (Japanese Patent Laid-Open No. 63-158105), 3) The order of strength of affinity for the three components is third component> second component> The unit packing layer filled with the first adsorbent which is the first component and the order of the strength of the affinity for the three components are: second component> third component>
By alternately passing at least four layers and the unit packed bed filled with the second adsorbent, which is the first component, to the simulated moving bed device connected in series in an endless manner, the adsorption characteristics of the three components differ. A method for fractionating and separating these (JP-A-64-
Although a known method such as 80409) can be adopted, the method of 1) above is preferable because it can be efficiently separated into three components and has a large processing capacity.

Hereinafter, the method 1) will be described with reference to FIG. 1. A large number of unit packing layers 1 to 10 filled with a solid adsorbent are connected in an endless series to form a circulation channel. 1
Reference numeral 3 is a connecting pipe that connects the packed bed 1 and the packed bed 10. A fixed amount of a raw material liquid f containing three components having different affinities for the adsorbent (the first component, the second component, and the third component) is introduced from the raw material liquid supply pipe 14e, and the eluting water D is supplied at the same time. It is introduced from the pipe 14d and flows into the circulation flow path. 12
Is a pump for circulating fluid. The raw material liquid f flows into the circulation passage through the pipe having the raw material liquid supply valve 5e, and the elution water D flows into the circulation passage through the pipe having the elution water supply valve 1d.

As the raw material liquid passes through each packed bed and moves in the circulation flow path, an adsorption zone is formed which is sequentially divided from a component having a weak affinity to the adsorbent to a component having a strong affinity. The component having a weak affinity is a trisaccharide or higher saccharide (third component), the strong component is a monosaccharide (first component), and the component (intermediate component) located in between is a disaccharide (second component). Is. When this intermediate component is present in the packed bed 4, the shut-off valve 11
Is closed, and a certain amount of the raw material liquid f is introduced from the raw material liquid supply pipe 14e, and is flown into the circulation flow path through the pipe having the raw material liquid supply valve 5e. Next, the extraction valve 4b is opened, and the intermediate component (second component) is extracted from the packed bed 4 to the outside of the system through the extraction pipe 14b.

After that, the shut-off valve 11 is opened, and the components having weak affinity (third component) remaining in the system while circulating the system are opened by sequentially opening the respective withdrawal valves 1a to 10a.
The extraction pipe 1 is further extracted outside the system, and at the same time, the extraction valve 1c to 10c is sequentially opened to open the extraction component 1 (first component) having a strong affinity.
Pull out from the system from 4c. In this way, the three components of the monosaccharide C as the first component, the disaccharide B as the second component, and the trisaccharide or higher saccharide A as the third component are separated.
2d to 10d are eluent water supply valves, and 2b and 3b are second
The extraction valves of the components are shown respectively.

As the adsorbent packed in the packed bed, an alkali metal type or alkaline earth metal type strongly acidic cation exchange resin is typically used.

The above chromatographic separation operation is carried out at 50 ° C. or higher,
It is preferably carried out under the temperature condition of 60 to 85 ° C. If the temperature is lower than 50 ° C., the viscosity of the raw material liquid is too high, the separation speed becomes slow, and the problem of spoilage of the raw material liquid due to generation of bacteria occurs. In order to operate under the above temperature conditions, it is necessary to heat the raw material liquid to 50 ° C. or higher before introducing it into the system.
Further, it is necessary to provide the chromatographic separation device with a constant temperature means for heating and maintaining the system constituted by the packed bed and the flow path tube at 50 ° C. or higher.

The third component solution (a saccharide solution of trisaccharides or more) obtained by the chromatographic separation is then sent to the concentration step. An overall process diagram of the present invention comprising a chromatographic separation process and a concentration process is shown in FIGS. 2 and 3.

In the figure, 20 is a chromatographic separation device, and 21 is an eluting water producing device. A known ion exchange resin device or a reverse osmosis membrane device is used as the eluting water producing device 21, and the eluting water is produced from raw water such as city water or industrial water by the device. As described above, the saccharides of the raw material liquid are separated into three components of the first component C, the second component B and the third component A by the chromatographic separation, and the third component A among them is the third component outflow line 22.
And is sent to the reverse osmosis membrane device 23.

In the process of producing a mixed solution containing three components, an inorganic salt such as sodium chloride may be mixed and mixed in the mixed solution. In this case, saccharides of three or more trisaccharides separated by chromatographic separation are mixed. Usually, it is usually brought into the solution (third component) with the inorganic salts mixed therein. As described below, the present invention circulates and uses the permeate separated by the reverse osmosis membrane device as eluent,
The content of the inorganic salt in the permeate becomes a problem. The allowable content of inorganic salts for use as elution water is 0.2% by weight or less. Therefore, the content of inorganic salts is 0.2% by weight
If it exceeds, it is necessary to remove inorganic salts using a separate reverse osmosis membrane device.

FIG. 2 shows the case where the inorganic salt removal treatment is not performed, and FIG. 3 shows the case where the inorganic salt removal treatment is performed using a separate reverse osmosis membrane device. In the reverse osmosis membrane device 23 shown in FIG. 2, the salt blocking rate of the reverse osmosis membrane is 90 to 93%.
The intermediate RO film is used, and specific examples of the intermediate RO film include NTR-729HF and the like.

The reverse osmosis membrane device 23 is provided with a constant temperature means for keeping the temperature at 50 ° C. or higher, and the concentration treatment is performed under the temperature condition of 50 ° C. or higher, preferably 50 to 70 ° C. If the temperature is lower than 50 ° C., the viscosity of the third component solution is too high, the separation rate (concentration processing rate) becomes slow, and the third component solution may be spoiled due to the generation of bacteria. Since the treatment is carried out at a temperature of 50 ° C. or higher, the reverse osmosis membrane is required to have heat resistance.

A saccharide solution containing three or more sugars (third component A) is 5
While being maintained at a temperature of 0 ° C. or higher, it is pressurized by the high pressure pump 24 through the third component outflow line 22, flows into the reverse osmosis membrane device 23, and is concentrated by the reverse osmosis membrane at a temperature of 50 ° C. or higher. , And is separated into a concentrated liquid and a permeated liquid (water). At the time of this concentration step, the third component solution (concentration about 4 to 5%) after chromatographic separation is concentrated about 4 to 8 times and the concentration about 2
A 0-30% concentrate is obtained.

The concentrate passes through the outflow line 25 and the evaporator 2
The permeated liquid is sent to the No. 6 and the permeated liquid passes through the eluting water circulation line 27 and joins with the eluting water supply line 28, and is then returned to the eluting water supply unit to be used as a part of the eluting water in the chromatographic separation device. In this case, since the reflux liquid has a temperature of room temperature or higher, it is possible to save heat energy for heating the eluting water. When the concentration cannot be concentrated to the desired concentration by one-time concentration treatment, the concentrated liquid may be introduced into the concentrated liquid circulation line 29, passed through the reverse osmosis membrane device 23 again, and repeatedly concentrated.

The third component solution introduced into the evaporator 26 is heated here, and the second-stage concentration treatment by evaporative concentration is performed. The evaporator 26 has a known structure. Since the third component solution is pre-concentrated by the reverse osmosis membrane device 23, a small-sized evaporator is sufficient. Also in this case, since the third component solution has a temperature of room temperature or higher, it is possible to save heat energy for evaporative concentration.

In this evaporative concentration step, the third component solution after concentration by the reverse osmosis membrane is concentrated to about 2.3 to 3.5 times to be a concentrated solution having a concentration of about 70%, and thus the desired high concentration is obtained. It is possible to obtain a saccharide having a trisaccharide or higher concentration.

As described above, when the content of the inorganic salts in the third component solution exceeds 0.2% by weight, the inorganic salts are removed by using a separate reverse osmosis membrane device as shown in FIG. There is a need. In this case, as the reverse osmosis membrane in the first reverse osmosis membrane device 30, a loose RO with a salt inhibition rate of 70% or less is used.
Membranes are used. By using this loose RO membrane, water and inorganic salts permeate the membrane and are separated from the concentrate. As a specific example of the loose RO film used when a large amount of monovalent inorganic salts are used as the inorganic salts, NTR-7250 or the like is used, and the loose RO film used when a large amount of divalent inorganic salts is used in addition to monovalent inorganic salts. As a specific example of the membrane, NTR-725
0, NTR-7450 and the like.

The concentrated liquid separated by the first reverse osmosis membrane device 30 is sent to the evaporation device 26 through the outflow line 25, while the permeated liquid (water containing inorganic salts) passes through the outflow line 32 and becomes the raw water ( It merges with an inflow line 33 for supplying city water or industrial water and flows into the second reverse osmosis membrane device 31. As the reverse osmosis membrane in the second reverse osmosis membrane device 31, a tight RO membrane having a salt rejection rate of 98% or more is used, and specific examples of the tight RO membrane include NTR-759HR and the like. Water and inorganic salts are separated by this reverse osmosis membrane device, and water as a permeate passes through the eluting water circulation line 34 and is returned to the eluting water supply section of the chromatographic separating device 20 to be used as eluting water.

[0034]

The present invention will be described in detail below with reference to examples, but the present invention is not limited to the examples. Example Using starch as a raw material, a liquefaction treatment was performed using a liquefying enzyme containing α-amylase as a main component, and a saccharification treatment was performed using a saccharifying enzyme containing glucoamylase and β-amylase as the main components. An aqueous starch sugar solution was obtained. Aqueous starch sugar composition Glucose: 41.1% Maltose: 42.4% Sugars of three or more sugars (mixture of maltotriose, maltotetraose, etc.): 16.3% Inorganic salts: 0.2%

The solid content concentration of this starch sugar aqueous solution was measured and found to be 60%. Chromatographic separation was performed using the above starch sugar aqueous solution as a raw material liquid by a simulated moving bed type multi-component separator shown in FIG. The operating conditions for chromatographic separation are as follows. Chromatographic separation conditions Adsorbent: Amberlite CG-6000 (trade name), Na-type eluent water: After starting the chromatographic separation device, use ion-exchanged water until a separated liquid is produced. After the separated liquid is generated, the separated liquid is concentrated by reverse osmosis membrane to obtain a membrane permeated liquid, and then a mixture of ion exchanged water and the membrane permeated liquid is used. Ion-exchanged water / membrane permeation liquid mixing ratio = 1.0: 0.6 Treatment temperature: 63 ° C Inflow amount of raw material liquid: 150 m ³ / D

By chromatographic separation, a target separation liquid (a saccharide solution of trisaccharides or more) was obtained. The content of trisaccharides or higher saccharides in this separation liquid was 98.5% (in addition, glucose was 0.8% and maltose was 0.7%), and sufficient separation performance was recognized. The solid content concentration of this separated solution was measured to be 5.1%, and the electric conductivity was 450 μS /
It was cm.

The separated liquid was sent to the reverse osmosis membrane apparatus according to the process shown in FIG. 2 and concentrated under the following conditions. Reverse osmosis membrane concentration conditions Reverse osmosis membrane used: NTR-729HF heat resistant product Treatment temperature: 60 ° C Pressure: 17 kg / cm ²

When the solid content concentration of the concentrated liquid was measured, it was 2
It was 5.5%. The permeated liquid separated from the concentrated liquid was refluxed to the eluting water supply unit of the chromatographic separation device and reused as chromatographic eluting water. The saccharide content in the permeate was all below the detection limit and could not be measured. Incidentally, the electric conductivity of this permeated liquid was 10 μS / cm. Therefore, it was confirmed that the permeated liquid can be sufficiently reused as chromatographic elution water.

The concentrated liquid is a small evaporator (evaporator)
The mixture was sent to the reactor and heated to evaporate and concentrate. The conditions of evaporative concentration are as follows. Evaporative concentration conditions Heating temperature: 80 ° C Heat source: Steam evaporator internal volume: 40 liters

After the completion of the concentration treatment, the concentration of the solid content of the concentrated liquid was measured to be 70%, and a syrup-like product of good quality was obtained. No deterioration or spoilage of the sugar solution was observed during the entire process.

Comparative Example Except that the separated liquid obtained by the chromatographic separation (a saccharide solution of trisaccharides or more) was directly sent to a large evaporator (internal volume of 300 liters) for concentration without performing concentration treatment by a reverse osmosis membrane device. Then, the treatment was carried out under the same conditions as in the above-mentioned Examples of the present invention to obtain a product having a solid content concentration of 70%.

(Comparison of equipment costs, etc.) The equipment costs (excluding the chromatographic separation device), the amount of waste water and the amount of steam used for the method of the present invention (example) and the conventional method (comparative example) are The cost was calculated, and the amount of wastewater and steam used was calculated from the data during operation, and the ratio of the two was compared. The results are as follows (in each case, the ratio was determined with the value in the conventional method being 1.0). Ratio of equipment costs: Conventional method: Method of the present invention = 1.0: 0.60 Ratio of wastewater: Conventional method: Method of the present invention = 1.0: 0.67 Ratio of steam usage: Conventional method: Method of the present invention = 1.0: 0.2

From the above points, it was confirmed that the method of the present invention requires less equipment cost than the conventional method and can reduce both the amount of waste water and the amount of steam used, and is economically extremely advantageous.

[0044]

As described above, according to the present invention, the chromatographically separated liquid (a saccharide solution of trisaccharides or more) is concentrated by the reverse osmosis membrane device as the first-stage concentration treatment, and then the second-stage concentration treatment. As a result, the chromatographic separation liquid can be concentrated more efficiently than the conventional method in which the chromatographic separation liquid is concentrated only by the evaporative concentration means, and the heat energy consumption required to obtain the target concentration Can be significantly reduced. Further, since the evaporator can be small in size, the equipment can be simplified as a whole, the equipment cost can be reduced, and it is economically extremely advantageous, and there is an effect that it can contribute to the reduction of the production cost.

In the present invention, the chromatographic separation operation and the reverse osmosis membrane concentration operation are performed under a temperature condition of 50 ° C. or higher, so that the chromatographic separation rate and the reverse osmosis membrane separation rate can be improved, and the viscosity of the sugar solution affects the separation rate. You can solve the problems that you give. Further, by treating under the above temperature conditions, generation of bacteria can be suppressed, and spoilage of the sugar solution can be prevented in advance.

In the present invention, since the permeated liquid separated in the reverse osmosis membrane concentration step is returned to the eluting water supply section of the chromatographic separation device, the permeated liquid can be effectively used as the eluting water. It is possible to reduce the amount of raw water used for producing elution water such as water.

According to the method of the present invention as defined in claim 2,
When the raw material liquid contains a considerable amount of inorganic salts, the permeated liquid separated by the reverse osmosis membrane device is further guided to the second reverse osmosis membrane device and separated into an inorganic salt solution and a permeated liquid. However, the inorganic salts can be surely separated and removed from the permeate, and there is no problem in reusing the permeate as eluent.

[Brief description of drawings]

FIG. 1 is a schematic view of a simulated moving bed multi-component separation device.

FIG. 2 is an explanatory view showing steps of the method of the present invention according to claim 1.

FIG. 3 is an explanatory view showing the steps of the method of the present invention according to claim 2.

[Explanation of symbols]

 1-10 Packed bed 14a-14c Extraction pipe 14d Elution water supply pipe 14e Raw material liquid supply pipe 20 Chromatographic separation device 23 Reverse osmosis membrane device 26 Evaporator 27, 34 Elution water circulation line 30 First reverse osmosis membrane device 31 Second Reverse osmosis device

Claims (2)

[Claims] 1. A mixed solution containing a monosaccharide and / or betaine (first component), a disaccharide (second component), and a saccharide (third component) formed by glycosidic bonding of three or more monosaccharide molecules to each other. Under a temperature condition of ℃ or more, a step of separating the above-mentioned first component, second component and third component into three components by a chromatographic separation device, and a third component solution obtained by the chromatographic separation
A step of concentrating with a reverse osmosis membrane device under a temperature condition of 0 ° C. or higher to separate into a concentrate and a permeate, a step of refluxing the permeate to an eluting water supply part of a chromatographic separator, and the above concentrate And a step of performing a second-stage concentration treatment by evaporating and condensing the saccharide. A method for producing a saccharide, wherein three or more monosaccharide molecules are glycosidic-bonded. 2. A mixed solution containing a monosaccharide and / or betaine (first component), a disaccharide (second component), and a saccharide (third component) formed by glycosidic bonding of three or more monosaccharide molecules to each other. Under a temperature condition of ℃ or more, a step of separating the above-mentioned first component, second component and third component into three components by a chromatographic separation device, and a third component solution obtained by the chromatographic separation
A step of concentrating with a first reverse osmosis membrane device under a temperature condition of 0 ° C. or higher to separate a concentrate and a permeate, and introducing the permeate to a second reverse osmosis membrane device to form an inorganic salt solution. A step of separating the permeate into a permeate, a step of refluxing the permeate separated by the second reverse osmosis membrane device to an eluent water supply section of the chromatographic separator, and a concentrate separated by the first reverse osmosis membrane device. And a step of performing a second-stage concentration treatment by evaporating and condensing the saccharide. A method for producing a saccharide, wherein three or more monosaccharide molecules are glycosidic-bonded.