JPH0670783A - Decomposition of polyolefin polymer compound - Google Patents
Decomposition of polyolefin polymer compoundInfo
- Publication number
- JPH0670783A JPH0670783A JP31155091A JP31155091A JPH0670783A JP H0670783 A JPH0670783 A JP H0670783A JP 31155091 A JP31155091 A JP 31155091A JP 31155091 A JP31155091 A JP 31155091A JP H0670783 A JPH0670783 A JP H0670783A
- Authority
- JP
- Japan
- Prior art keywords
- polymer compound
- polyolefin polymer
- polyolefin
- wood
- basidiomycete
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229920000098 polyolefin Polymers 0.000 title claims abstract description 30
- 150000001875 compounds Chemical class 0.000 title claims abstract description 29
- 229920000642 polymer Polymers 0.000 title claims abstract description 29
- 238000000354 decomposition reaction Methods 0.000 title claims description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 32
- 241000221198 Basidiomycota Species 0.000 claims abstract description 21
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 16
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000012254 powdered material Substances 0.000 claims 1
- -1 polyethylene Polymers 0.000 abstract description 17
- 239000004698 Polyethylene Substances 0.000 abstract description 16
- 229920000573 polyethylene Polymers 0.000 abstract description 16
- 230000003068 static effect Effects 0.000 abstract description 3
- 241000222393 Phanerochaete chrysosporium Species 0.000 abstract description 2
- 238000004458 analytical method Methods 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 229920003023 plastic Polymers 0.000 abstract description 2
- 239000004033 plastic Substances 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract 1
- 244000005700 microbiome Species 0.000 description 8
- 239000007787 solid Substances 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 5
- 241000233866 Fungi Species 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- 239000013502 plastic waste Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 3
- 235000011130 ammonium sulphate Nutrition 0.000 description 3
- 239000013028 medium composition Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 241000222640 Polyporus Species 0.000 description 2
- 241001061969 Porodisculus Species 0.000 description 2
- 241001674251 Serpula lacrymans Species 0.000 description 2
- 241000222355 Trametes versicolor Species 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 102220201851 rs143406017 Human genes 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 241000228245 Aspergillus niger Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 102100034279 Calcium-binding mitochondrial carrier protein Aralar2 Human genes 0.000 description 1
- 241000123346 Chrysosporium Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000222336 Ganoderma Species 0.000 description 1
- 240000000599 Lentinula edodes Species 0.000 description 1
- 235000001715 Lentinula edodes Nutrition 0.000 description 1
- 244000162269 Lentinus lepideus Species 0.000 description 1
- 235000017066 Lentinus lepideus Nutrition 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241001599571 Serpula <basidiomycete> Species 0.000 description 1
- 241000222646 Stereum Species 0.000 description 1
- 241000222354 Trametes Species 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229920002988 biodegradable polymer Polymers 0.000 description 1
- 239000004621 biodegradable polymer Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 108010084210 citrin Proteins 0.000 description 1
- 238000001983 electron spin resonance imaging Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、ポリオレフィン系高分
子化合物の分解法に関するものであり、更に詳細には、
高分子ポリエチレン等を強力に分解できる新しい工業的
方法に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for decomposing a polyolefin polymer compound, more specifically,
The present invention relates to a new industrial method capable of strongly decomposing high-molecular polyethylene and the like.
【０００２】したがって本発明は、現在その処理が大き
な社会問題となっているプラスチック廃棄物の処理に大
きな貢献をなすのみでなく、その分解メカニズムを解明
することにより、生分解性高分子化合物のデザインにも
大きな期待をつなぐものである。Therefore, the present invention not only makes a great contribution to the treatment of plastic waste, whose treatment is now a major social problem, but also elucidates the decomposition mechanism to design biodegradable polymer compounds. It is also a great expectation.
【０００３】[0003]
【従来の技術】従来、ポリオレフィン系高分子化合物は
生分解を受けないとされており、ポリオレフィン系高分
子化合物の一つであるポリエチレンは、１０年間菌処理
を受けても１％程度しか分解されないことが知られてい
る（Ｊｏｕｒｎａｌ ｏｆ Ａｐｐｌｉｅｄ Ｐｏｌｙ
ｍｅｒ Ｓｃｉｅｎｃｅ，３５，１２８９−１３０２
（１９８８））。2. Description of the Related Art Hitherto, it has been said that polyolefin-based polymer compounds are not biodegradable, and polyethylene, which is one of the polyolefin-based polymer compounds, is degraded only to about 1% even after being treated with bacteria for 10 years. Is known (Journal of Applied Poly)
mer Science, 35 , 1289-1302.
(1988)).
【０００４】[0004]
【発明が解決しようとする課題】プラスチック廃棄物処
理で問題となるのは、速やかにプラスチック廃棄物を分
解せしめることであって、従来法では上記したことから
も明らかなように、目的を達成することは出来ない。A problem in the treatment of plastic waste is to promptly decompose the plastic waste, and the conventional method achieves the object, as is clear from the above. I can't do that.
【０００５】[0005]
【課題を解決するための手段】本発明は、このような技
術の現状に鑑み、プラスチック公害の防止を目的として
なされたものであって、従来法では実質的に分解するこ
とのできなかったポリオレフィン系高分子化合物を効率
よく分解することのできる方法を開発する目的でなされ
たものである。SUMMARY OF THE INVENTION The present invention has been made in view of the present state of the art in order to prevent plastic pollution, and is a polyolefin that cannot be substantially decomposed by conventional methods. It was made for the purpose of developing a method capable of efficiently decomposing a polymer compound.
【０００６】上記目的を達成するために検討を行い、二
次公害を防止するという観点から、種々の微生物を用い
る生物処理に着目した。しかしながら、所期の目的を達
成するには至らなかったので、微生物の選択、培養条
件、処理条件等について発想の大転換の必要性を認め
た。[0006] In order to achieve the above object, studies have been conducted, and attention has been paid to biological treatment using various microorganisms from the viewpoint of preventing secondary pollution. However, it was not possible to achieve the intended purpose, and therefore, it was necessary to make a big change in idea regarding selection of microorganisms, culture conditions, treatment conditions and the like.
【０００７】そこで、微生物の生育やその処理に必要な
栄養成分である窒素源及び／または炭素源について、従
来の常識とは全く逆にこれをカットし、また、ポリオレ
フィン系高分子化合物を親水化して微生物処理したとこ
ろ、木材腐朽性担子菌が効率よくポリオレフィン系高分
子化合物を分解することを認め、本発明を完成するに至
った。以下本発明を詳述するが、本明細書において、ポ
リオレフィン系高分子化合物をポリエチレンという場合
もある。Therefore, nitrogen sources and / or carbon sources, which are nutrient components necessary for the growth and treatment of microorganisms, are cut completely contrary to the conventional wisdom, and the polyolefin polymer compound is hydrophilized. As a result of the microbial treatment, it was confirmed that the wood-destroying basidiomycete efficiently decomposed the polyolefin polymer compound, and the present invention was completed. The present invention is described in detail below, but in this specification, the polyolefin-based polymer compound may be referred to as polyethylene.
【０００８】本発明を実施するには、分解処理しようと
するポリオレフィン系高分子化合物を、木材腐朽性担子
菌と接触させる必要があるが、その場合、窒素及び／ま
たは炭素を制限した環境下におくと更に効率的に分解処
理が行われる。In order to carry out the present invention, it is necessary to bring the polyolefin polymer compound to be decomposed into contact with wood-destroying basidiomycetes, but in that case, under an environment in which nitrogen and / or carbon is restricted. If it is set, the decomposition process is performed more efficiently.
【０００９】本発明においては、マツオウジ（Ｌｅｎｔ
ｉｎｕｓ ｌｅｐｉｄｅｕｓ）等褐色腐朽性担子菌を含
む木材腐朽性担子菌が広く使用できるが、特に白色腐朽
性担子菌つまりリグニン分解菌が有利に利用できる。In the present invention, pine tree (Lent)
Wood-destroying basidiomycetes including brown-rot-destroying basidiomycetes such as inus lepideus) can be widely used, and particularly white-rotting basidiomycetes, that is, lignin-degrading bacteria can be advantageously used.
【００１０】これら木材腐朽性担子菌としては、次のよ
うな各属に属する微生物が広く例示される：コリオラス
属（Ｃｏｒｉｏｌｕｓ ｖｅｒｓｉｃｏｌｏｒ ＩＦＯ
７０４３等）、ファネロケーテ属（Ｐｈａｎｅｒｏｃ
ｈａｅｔｅ ｃｈｒｙｓｏｓｐｏｒｉｕｍ ＡＴＣＣ
３４５４１等）、トラメデス属（Ｔｒａｍｅｔｅｓｄｉ
ｃｋｉｎｓｉｉ ＩＦＯ ６４８８等）、ポリポラス属
（Ｐｏｌｙｐｏｒｕｓ ｍｉｋａｄｏｉ ＩＦＯ ６５
１７等）ステレウム属（Ｓｔｅｒｅｕｍ ｆｒｕｓｔｕ
ｌｏｓｕｍ ＩＦＯ ４９３２等）、ガノデルマ属（Ｇ
ａｎｏｄｅｒｍａ ａｐｐｌａｎａｔｕｍ ＩＦＯ ６
４９９等）、レンチテス属（Ｌｅｎｚｉｔｅｓ ｂｅｔ
ｕｌｉｎａ ＩＦＯ ８７１４等）、ホーメス属（Ｆｏ
ｍｅｓｆｏｍｅｎｔａｒｉｕｓ ＩＦＯ ３０３７１
等）、ポロディスキュラス属（Ｐｏｒｏｄｉｓｃｕｌｕ
ｓ ｐｅｎｄｕｌｕｓ ＩＦＯ ４９６７等）、レンチ
ヌス属（Ｌｅｎｔｉｎｕｓ ｅｄｏｄｅｓ ＩＦＯ ３
１３３６、Ｌ．ｌｅｐｉｄｅｕｓ ＩＦＯ ７０４３
等）、セルプラ属（Ｓｅｒｐｕｌａ ｌａｃｒｙｍａｎ
ｓ ＩＦＯ ８６９７等）その他。As these wood-destroying basidiomycetes, microorganisms belonging to the following genera are widely exemplified: Coriolus versicolor IFO.
7043 etc.), genus Phanelocete (Phaneroc)
haete chrysosporium ATCC
34541), and the genus Trametes (Trametesdi
ckinsii IFO 6488 etc., Polyporus genus (Polyporus mikadoi IFO 65
17 etc.) Stereum fruitus
losum IFO 4932 etc.), Ganoderma (G
anoderoma applanatum IFO 6
499), Lentites bet
ulina IFO 8714 etc.), genus (Fo)
mesfomentarius IFO 30371
Etc.), Porodisculus (Porodisculus)
s pendulus IFO 4967, etc., Lentinus edodes IFO 3
1336, L.S. lepideus IFO 7043
Etc.), genus Serpula (Serpula lacryman)
s IFO 8697 etc.) Others.
【００１１】本発明において使用可能な木材腐朽性担子
菌としては、上記した微生物のほか、ＮＫ−１１４８株
（ＦＥＲＭ ＢＰ−１８５９）も使用することができ、
本菌株はポリオレフィン系高分子化合物を高度に分解す
ることができる。このＮＫ−１１４８株の菌学的性質の
詳細については、特開平２−２５９１８０号に開示され
ている。As the wood-destroying basidiomycetes usable in the present invention, in addition to the above-mentioned microorganisms, NK-1148 strain (FERM BP-1859) can be used.
This strain is capable of highly decomposing polyolefin polymer compounds. Details of the mycological properties of the NK-1148 strain are disclosed in JP-A-2-259180.
【００１２】本発明においては、ポリオレフィン系高分
子化合物を上記した１種またはそれ以上の木材腐朽性担
子菌で処理して分解することを特徴とするのであるが、
この場合、窒素及び／または炭素制限下において処理す
ると更に効率的である。The present invention is characterized in that a polyolefin polymer compound is treated with one or more of the above-mentioned wood-destroying basidiomycetes and decomposed.
In this case, it is more efficient to treat under nitrogen and / or carbon limitation.
【００１３】具体的にはポリオレフィン系高分子化合物
を、好ましくは窒素及び／または炭素を制限した状態
で、木材腐朽性担子菌と接触させ、至適温度例えば１５
〜３５℃で放置すれば、５〜３０日間程度でポリオレフ
ィン系高分子化合物をきわめて効率的に分解することが
できるのである。Specifically, the polyolefin-based polymer compound is contacted with a wood-destroying basidiomycete, preferably in a state where nitrogen and / or carbon is restricted, and the optimum temperature is, for example, 15
If left at ˜35 ° C., the polyolefin polymer compound can be decomposed very efficiently in about 5 to 30 days.
【００１４】この場合、微生物で処理するにもかかわら
ず、窒素及び／または炭素を制限することが重要であ
る。窒素及び／または炭素を可及的少量、好ましくは含
有しないのが良い。窒素および炭素以外の栄養源につい
ては、格別の制限はなく、木材腐朽性担子菌の生育に常
用される各成分が適宜使用される。本発明においては上
記の窒素及び／または炭素に関する条件が満たされれ
ば、初期の目的が達成されるので、窒素源や炭素源を完
全にカット状態、例えば水に（必要あれば寒天及び／ま
たはｐＨ調節剤等は添加する）ポリオレフィン系高分子
化合物と木材腐朽性担子菌を加えてインキュベートする
ことによっても、ポリオレフィン系高分子化合物を分解
することができる。In this case, it is important to limit nitrogen and / or carbon despite treatment with microorganisms. It is preferable not to contain nitrogen and / or carbon in the smallest possible amount, and preferably to contain no carbon. There are no particular restrictions on nutrient sources other than nitrogen and carbon, and each component commonly used for growing wood-destroying basidiomycetes is appropriately used. In the present invention, if the above-mentioned conditions concerning nitrogen and / or carbon are satisfied, the initial purpose can be achieved, so that the nitrogen source and the carbon source are completely cut, for example, in water (agar and / or pH if necessary). The polyolefin-based polymer compound can also be decomposed by adding and incubating the polyolefin-based polymer compound and wood-destroying basidiomycete.
【００１５】また、本発明においては、木材腐朽性担子
菌を使用するのであるが、菌自体のほか、その培養物及
び／またはその処理物も使用することができる。該培養
物とは、菌を培養して得た菌体及び培養液の混合物を広
く指すが、本発明においては、菌体培養物から分離した
ウェットケーキ等の菌体、その残渣、及び、固体物をす
べて除去した後の培養液を利用することもできる。また
その処理物とは、上記したものを濃縮、乾燥、または希
釈したものをすべて指すものである。In the present invention, the wood-destroying basidiomycetes are used, but in addition to the fungi themselves, cultures and / or processed products thereof can also be used. The culture broadly refers to a mixture of cells and a culture solution obtained by culturing a bacterium, but in the present invention, cells such as wet cake separated from the cell culture, its residue, and solids. It is also possible to use the culture medium after removing all the substances. Further, the processed product refers to all of the above-mentioned products which are concentrated, dried or diluted.
【００１６】本発明にしたがってポリオレフィン系高分
子化合物を分解処理するに際して、微生物やそれから生
産される酵素等と接触しやすくするために、親水化して
用いるのが好適である。ポリオレフィン系高分子化合物
の親水化処理は、常用される界面活性剤等を塗布及び／
または混合する方法、無機物あるいは有機物を塗布及び
／または混合する方法などを用いることができる。な
お、これらのポリオレフィン系化合物を細砕、粉末化し
たり、あるいは細孔膜化すると更に好適である。When the polyolefin polymer compound is decomposed according to the present invention, it is preferably hydrophilized for easy contact with microorganisms and enzymes produced therefrom. The hydrophilic treatment of the polyolefin-based polymer compound is performed by applying and / or applying a commonly used surfactant or the like.
Alternatively, a mixing method, a method of applying and / or mixing an inorganic material or an organic material, or the like can be used. It is more preferable to pulverize or powderize these polyolefin-based compounds, or to form a porous film.
【００１７】本発明によれば、高圧ポリエチレン、低中
圧ポリエチレンその他各種のポリエチレン、アイソタク
チックその他各種のポリプロピレンのほか、ポリオレフ
ィン系高分子化合物であればすべて分解することができ
る。もちろん、これらの混合物も生分解可能である。以
下、本発明の実施例について述べる。According to the present invention, in addition to high-pressure polyethylene, low-medium-pressure polyethylene and other types of polyethylene, isotactic and other types of polypropylene, any polyolefin-based polymer compound can be decomposed. Of course, these mixtures are also biodegradable. Examples of the present invention will be described below.
【００１８】[0018]
【実施例１】親水性を有するポリエチレン膜（旭化成社
製ハイポアＰＥ−１１００）を窒素を含まない固体培地
（ＫＨ2ＰＯ4 １．０ｇ、ＮａＨ2ＰＯ4 ０．２ｇ、Ｍ
ｇＳＯ4・７Ｈ2Ｏ ０．１ｇ、ＺｎＳＯ4・７Ｈ2Ｏ
０．０１ｍｇ、ＣｕＳＯ4・５Ｈ2Ｏ ０．０２ｍｇ、ｇ
ｌｕｃｏｓｅ ２０ｇ、寒天３０ｇ、水１リットル）上
に置き各種微生物（白色腐朽性担子菌；Ｐｈａｎｅｒｏ
ｃｈａｅｔｅ ｃｈｒｙｓｏｓｐｏｒｉｕｍ ＡＴＣＣ
３４５４１、Ｃｏｒｉｏｌｕｓ ｖｅｒｓｉｃｏｌｏ
ｒ ＩＦＯ ７０４３、ＮＫ−１１４８ ＦＥＲＭ Ｂ
Ｐ−１８５９、褐色腐朽性担子菌；Ｌｅｎｔｉｎｕｓ
ｌｅｐｉｄｅｕｓ ＩＦＯ ７０４３、Ｓｅｒｐｕｌａ
ｌａｃｒｙｍａｎｓ ＩＦＯ ＥＰＲＩ ６３５２、
不完全菌；Ａｓｐｅｒｇｉｌｌｕｓ ｎｉｇｅｒ ＩＦ
Ｏ ６３４１、Ｐｅｎｉｃｉｌｌｉｕｍ ｃｉｔｒｉｎ
ｕｍ ＩＦＯ ６３５２、細菌；Ｂａｃｉｌｌｕｓ ｓ
ｕｂｔｉｌｌｉｓ ＩＦＯ ３１３４、Ｐｓｅｕｄｏｍ
ｏｎａｓ ｐａｕｃｉｍｏｂｉｌｌｉｓ ＳＹＫ−６）
を接種し２０〜２８℃で２０日間静置培養した。培養
後、ポリエチレン膜を水中に懸濁させ、その分散状態を
観察して、生分解性を評価した。また、高度に分解が認
められたポリエチレン膜については、ＧＰＣ（昭和電工
製カラムＫＳ−８０Ｍ；溶離液ＴＣＢ；流速１ｍｌ／ｍ
ｉｎ；温度１３０℃；検出器ＲＩ）で分析し、平均分子
量の変化で生分解性を評価した。その結果を下記の表１
に示す。Example 1 A polyethylene membrane having hydrophilicity (Hypore PE-1100 manufactured by Asahi Kasei Corp.) was used as a solid medium (KH 2 PO 4 1.0 g, NaH 2 PO 4 0.2 g, M) containing no nitrogen.
gSO 4 · 7H 2 O 0.1g, ZnSO 4 · 7H 2 O
0.01mg, CuSO 4 · 5H 2 O 0.02mg, g
Lucose 20 g, agar 30 g, water 1 liter) and put on various microorganisms (white rot basidiomycetes; Phanero
chaete chrysosporium ATCC
34541, Coriolus versicolor
r IFO 7043, NK-1148 FERM B
P-1859, brown-rot basidiomycete; Lentinus
lepideus IFO 7043, Serpula
lacrymans IFO EPRI 6352,
Imperfect bacteria; Aspergillus niger IF
O 6341, Penicillium citrin
um IFO 6352, bacteria; Bacillus
subtilis IFO 3134, Pseudom
onas paucimobillis SYK-6)
Was inoculated and statically cultivated at 20 to 28 ° C. for 20 days. After culturing, the polyethylene membrane was suspended in water and the dispersed state was observed to evaluate biodegradability. Further, regarding the polyethylene membrane which was highly decomposed, GPC (Showa Denko column KS-80M; eluent TCB; flow rate 1 ml / m)
in; temperature 130 ° C .; detector RI), and biodegradability was evaluated by the change in average molecular weight. The results are shown in Table 1 below.
Shown in.
【００１９】表１から明らかなように木材腐朽性担子菌
はポリエチレン膜を分解することができることが確認さ
れた。特にＮＫ−１１４８株を用いた場合が高度に分解
しており、ＧＰＣで分析した結果、従来全く分解するこ
とができなかった重量平均分子量１２５，０００のポリ
エチレンが重量平均分子量１０，３００に分解された。As is clear from Table 1, it was confirmed that wood-destroying basidiomycetes were capable of decomposing polyethylene membranes. Particularly when NK-1148 strain was used, it was highly degraded, and as a result of GPC analysis, polyethylene having a weight average molecular weight of 125,000, which could not be degraded at all, was degraded to a weight average molecular weight of 10,300. It was
【００２０】[0020]
【表１】 [Table 1]
【００２１】[0021]
【実施例２】実施例１によって認められた白色腐朽菌
（ＮＫ−１１４８）を用いて実施例１の処理条件に準
じ、窒素濃度の異なる固体培地（窒素濃度を硫酸アンモ
ニウムを用いて０ｇ／リットル、０．０５ｇ／リット
ル、０．１０ｇ／リットル、０．１５ｇ／リットルとし
他は実施例１と同様の培地組成である）で静置培養し、
実施例１に準じて分解性を評価した。その結果を表２に
示す。[Example 2] Using the white-rot fungus (NK-1148) recognized in Example 1, solid media having different nitrogen concentrations were used according to the treatment conditions of Example 1 (nitrogen concentration was 0 g / l using ammonium sulfate, Static culture at 0.05 g / liter, 0.10 g / liter, and 0.15 g / liter except that the medium composition is the same as in Example 1),
The degradability was evaluated according to Example 1. The results are shown in Table 2.
【００２２】[0022]
【表２】 [Table 2]
【００２３】[0023]
【実施例３】実施例１によって分解が認められた白色腐
朽菌（ＮＫ−１１４８）を用いて実施例１の処理条件に
準じ、炭素濃度の異なる固体培地（炭素濃度が０ｇ／リ
ットル、０．２ｇ／リットル、０．４ｇ／リットル、
８．０ｇ／リットルとなるようにグルコースをそれぞれ
添加し、硫酸アンモニウムを０．５８ｇ（Ｎ濃度０．１
５ｇ／リットル）とした以外は実施例１と同様の培地組
成である）で静置培養し、実施例１に準じて分解性を評
価した。その結果を下記の表３に示す。[Example 3] Using the white-rot fungus (NK-1148) whose decomposition was observed in Example 1, according to the treatment conditions of Example 1, solid media with different carbon concentrations (carbon concentration 0 g / liter, 0. 2 g / liter, 0.4 g / liter,
Glucose was added so that the concentration would be 8.0 g / liter, and 0.58 g of ammonium sulfate (N concentration: 0.1
5 g / liter) except that the medium composition was the same as in Example 1), and stationary culture was performed, and the degradability was evaluated according to Example 1. The results are shown in Table 3 below.
【００２４】[0024]
【表３】 [Table 3]
【００２５】[0025]
【実施例４】実施例１によって分解が認められた白色腐
朽菌（ＮＫ−１１４８）を用いて実施例１の処理条件に
準じ、窒素源及び炭素源を共に含まない固体培地（グル
コースを０ｇとした以外は実施例１と同様の培地組成で
ある）、および実施例１の培地から栄養源をすべて除い
た固体培地（寒天３０ｇ、水１リットル）の２種類の固
体培地で静置培養し、実施例１に準じて分解性を評価し
た。その結果を表４に示す。[Example 4] Using a white-rot fungus (NK-1148) whose decomposition was observed in Example 1, according to the treatment conditions of Example 1, a solid medium containing neither a nitrogen source nor a carbon source (glucose: 0 g Other than the above, the medium composition is the same as that in Example 1), and static culture is performed in two types of solid medium (30 g of agar, 1 liter of water) obtained by removing all nutrients from the medium of Example 1, The degradability was evaluated according to Example 1. The results are shown in Table 4.
【００２６】[0026]
【表４】 [Table 4]
【００２７】[0027]
【実施例５】実施例２において最大の分解能力を示した
窒素濃度の固体培地（硫酸アンモニウム０ｇ／リット
ル、他の条件は実施例１に準じる）で、白色腐朽菌（Ｎ
Ｋ−１１４８）を用い、ポリエチレン膜の親水化が分解
に及ぼす影響を調べた。試料として疎水性のポリエチレ
ン膜（旭化成製ハイポアＰＥ−２１００）およびこれを
界面活性剤処理（疎水性ポリエチレン膜を０．１％Ｔｗ
ｅｅｎ８０水溶液に２４時間浸漬）して親水性を付与し
たポリエチレン膜を用いた。なお、分解性は実施例１に
準じてＧＰＣで調べた。Example 5 In a solid medium having a nitrogen concentration that showed the maximum decomposing ability in Example 2 (ammonium sulfate 0 g / liter, other conditions are the same as in Example 1), white rot fungus (N
K-1148) was used to examine the effect of hydrophilization of the polyethylene membrane on the decomposition. As a sample, a hydrophobic polyethylene film (Hypore PE-2100 manufactured by Asahi Kasei) and a surfactant treatment (hydrophobic polyethylene film of 0.1% Tw) were used.
A polyethylene film having a hydrophilic property was obtained by immersion in an een80 aqueous solution for 24 hours). The degradability was examined by GPC according to Example 1.
【００２８】[0028]
【表５】 [Table 5]
【００２９】[0029]
【発明の効果】本発明によれば、ポリオレフィン系高分
子化合物を効率的に分解処理することができ、しかもそ
の際二次公害を引き起こすこともないので、本発明は、
現在その処理が世界的にも大きな社会問題となっている
プラスチック廃棄物の処理に大きな貢献をなすものであ
る。EFFECTS OF THE INVENTION According to the present invention, a polyolefin-based polymer compound can be efficiently decomposed, and at the same time, it does not cause secondary pollution.
At present, the treatment makes a great contribution to the treatment of plastic waste, which is a major social problem in the world.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.5 識別記号 庁内整理番号 ＦＩ 技術表示箇所 (Ｃ１２Ｐ 5/02 Ｃ１２Ｒ 1:07） (Ｃ１２Ｐ 5/02 Ｃ１２Ｒ 1:38） ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 5 Identification code Internal reference number FI technical display location (C12P 5/02 C12R 1:07) (C12P 5/02 C12R 1:38)
Claims (4)
またはその処理物により、ポリオレフィン系高分子化合
物を分解することを特徴とするポリオレフィン系高分子
化合物の分解法。1. A wood-destroying basidiomycete, a culture thereof, and / or
Alternatively, a polyolefin polymer compound is decomposed by a treated product thereof, which is a method for decomposing a polyolefin polymer compound.
び／または炭素を制限した培養条件下において、木材腐
朽性担子菌で処理することを特徴とするポリオレフィン
系高分子化合物の分解法。2. A method for decomposing a polyolefin-based polymer, which comprises treating the polyolefin-based polymer with a wood-destroying basidiomycete under a culture condition in which nitrogen and / or carbon is restricted.
あることを特徴とする請求項１〜請求項２のいずれか１
項の分解法。3. The wood-rotting basidiomycete is a white-rotting basidiomycete according to any one of claims 1 to 2.
Term decomposition method.
親水化及び／または粉末化、細孔膜化したものを使用す
ることを特徴とする請求項１〜請求項３のいずれか１項
の分解法。4. A polyolefin-based polymer compound,
The decomposition method according to any one of claims 1 to 3, wherein a hydrophilized and / or powdered material and a porous film is used.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP31155091A JPH0670783A (en) | 1991-10-31 | 1991-10-31 | Decomposition of polyolefin polymer compound |
| EP92922386A EP0596123A4 (en) | 1991-10-31 | 1992-10-30 | Biodegradable plastic. |
| PCT/JP1992/001411 WO1993009184A1 (en) | 1991-10-31 | 1992-10-30 | Biodegradable plastic |
| US08/078,296 US5378738A (en) | 1991-10-31 | 1992-10-30 | Biodegradable plastic |
| CA002099773A CA2099773A1 (en) | 1991-10-31 | 1992-10-30 | Biodegradable plastic |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP31155091A JPH0670783A (en) | 1991-10-31 | 1991-10-31 | Decomposition of polyolefin polymer compound |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0670783A true JPH0670783A (en) | 1994-03-15 |
Family
ID=18018592
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP31155091A Pending JPH0670783A (en) | 1991-10-31 | 1991-10-31 | Decomposition of polyolefin polymer compound |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0670783A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101305205B1 (en) * | 2006-03-16 | 2013-09-12 | 소니 주식회사 | Data transfer device and data transfer system |
| CN117018530A (en) * | 2023-06-06 | 2023-11-10 | 天津大学 | Use of overexpression of genes encoding alkane hydroxylase and genes encoding yeast endogenous transport proteins in degradation of polyethylene |
-
1991
- 1991-10-31 JP JP31155091A patent/JPH0670783A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101305205B1 (en) * | 2006-03-16 | 2013-09-12 | 소니 주식회사 | Data transfer device and data transfer system |
| CN117018530A (en) * | 2023-06-06 | 2023-11-10 | 天津大学 | Use of overexpression of genes encoding alkane hydroxylase and genes encoding yeast endogenous transport proteins in degradation of polyethylene |
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