JPH0662879A - Production of lysine - Google Patents

Abstract

PURPOSE:To provide a method for producing lysine by a fermentation method using a secondary squeezed liquid of squeezed less obtained by squeezing juice of fruit as a raw material. CONSTITUTION:A secondary squeezed liquid 9 produced in a juice producing process of fruits is subjected to centrifugal separation treatment to remove pulp and solid suspension and further subjected to ultrafiltration treatment to remove various germs and the resultant liquid is used as a raw material and subjected to lysine fermentation in a lysine fermentation process 10 to produce lysine.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention uses a secondary squeezing liquid of a squeezed lees obtained by squeezing juices of fruits containing sugar, such as apples, mandarins, and grapes, to produce lysine by fermentation. On how to do.

[0002]

2. Description of the Related Art Conventionally, a synthetic medium containing glucose has been used as a raw material for lysine fermentation, and there is no example in which lysine fermentation was carried out using a secondary juice of fruit as a raw material. In Figure 3,
The manufacturing process of the conventional apple juice is shown. In FIG. 3, 1
Is an apple, 2 is a squeezing process, 3 is a solid-liquid separation process, 4 is an apple juice, 5 is an apple squeezing lees, 6 is a secondary pressing process, 7 is a drying process, 8 is a feed, 9 is a secondary squeezing liquid. Indicates. As shown in FIG. 3, the apple juice 4 separated in the solid-liquid separation step 3,
The feed 8 obtained by drying the secondary pressed meal obtained from the apple pressed meal 5 in the secondary pressing step 6 in the drying step 7 was a product,
The secondary pressed juice 9 was discarded.

[0003]

[Problems to be Solved by the Invention] In lysine fermentation,
The use of synthetic medium containing glucose is costly and impractical. Further, conventionally, for example, apple secondary juice is discarded in the apple juice manufacturing process, but this secondary juice contains nutrients such as sugar and nitrogen.
It is well worth using. However, since suspended solids such as pulp are included, inconvenience such as sticking occurs during sterilization in the conventional fermentation process shown in Fig. 4, and the heat of sterilization causes the amino acids and sugars in the secondary juice to react with the Maillard reaction. There is a problem that it is decomposed by. In FIG. 4, 2
1 is a medium adjusting tank, 24 is a main fermentation tank, 25 is an inoculum culture tank,
26 is a pH controller, 27 is a defoaming controller, 2
8 is a removal filter, 29 is a blower, and 30 is a sterilizer.

In view of the above-mentioned state of the art, the present invention aims to provide a method for producing lysine by a fermentation method using the secondary squeezed juice of fruit free from the above-mentioned problems.

[0005]

[Means for Solving the Problems] According to the present invention, pulp and solid suspended matter are removed from a secondary squeezed liquid generated in a fruit juice production process of a fruit by centrifugal separation operation, and then bacteria are removed by an ultrafiltration operation. A method for producing lysine, which comprises subjecting the obtained liquid to a lysine fermentation method as a raw material.

[0006]

[Function] By centrifuging the secondary juice of fruits such as apples, suspended solids such as pulp are removed, and by using ultrafiltration, various bacteria can be removed, and it is not necessary to sterilize by steaming. It is possible to prevent denaturation due to heat. Also, by removing the suspended solids by centrifugation, the time for ultrafiltration can be shortened.

[0007]

DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described with reference to FIG.
In FIG. 1, an apple raw material 1 is squeezed in a squeezing step 2, and is separated into an apple juice 4 and an apple squeeze cake 5 through a solid-liquid separation step 3. The apple juice dregs 5 becomes a feed 8 through a pressing process 6 and a drying process 7. Also, the pressing step 6
Lysine is produced in the lysine fermentation step 10 by using the secondary juice 9 obtained in step 1 as a fermentation raw material, purified in the separation and purification step 11, and then added to the feed 8.

In the lysine fermentation step 10, as shown in FIG. 2, p of the apple secondary juice 9 is stored in the medium adjusting tank 21.
H was adjusted to 7.0 and pulp was separated by the centrifuge 22.
The bacteria are removed by the ultrafiltration device 23 and fed into the main fermentation tank 24 as a medium. After cooling the medium fed into the main fermentation tank 24, the inoculum preconditioned in the inoculum culture tank 25 is added to start fermentation. The pH during the fermentation is adjusted to pH 7.0 by the pH controller 26, and in the case of vigorous foaming, the defoaming controller 27 adds an antifoaming agent. For ventilation, air is sent from a blower 29, and a sterilization filter 28 sterilizes the bacteria. After the fermentation is completed, the entire fermentation liquid is drawn and sent to the separation and purification process. After withdrawing the entire amount, the main fermenter 24 is washed to prepare for the next fermentation.

[0009]

Industrial Applicability According to the present invention, it becomes possible to reduce the lysine production cost as compared with the conventional lysine fermentation using a synthetic medium containing glucose as a raw material, and the conventionally used secondary juice of fruit is used as a raw material. By the above, lysine produced can be added to the juice juice-meal feed, which is a by-product of the fruit juice production process, and livestock feed with higher added value can be obtained. Furthermore, by sterilizing using ultrafiltration, it becomes possible to prevent thermal denaturation of the secondary juice of juice.

[Brief description of drawings]

FIG. 1 is an explanatory diagram of a flow of an embodiment of the present invention.

FIG. 2 is an explanatory diagram of a flow of a lysine fermentation method in the flow of FIG.

FIG. 3 is an explanatory view of a flow of one aspect of a conventional fruit juice manufacturing process.

FIG. 4 is an explanatory view of a flow of the lysine fermentation method of the secondary juice of the flow of FIG.

Claims (1)

[Claims] 1. A pulp and a solid suspended matter are removed from a secondary squeezed liquid generated in a fruit juice production process by a centrifugal separation operation,
Further, a method for producing lysine, which comprises subjecting the obtained liquid to a lysine fermentation method after removing contaminants by an ultrafiltration operation.