JPH06148203A - Automatic analyzer - Google Patents

Abstract

PURPOSE:To enable items of both immunology and biochemistry to be measured once a sample is set and to shorten the measurement time by comprising a means for executing an immunological analyzing process which needs B/F separation and a biochemical process by colorimetry at the same time. CONSTITUTION:When power source is cast, the CPU reads program memories, stores test items and the like inputted from an operation part 9, and, after reaction tubes 5 are washed by a washing part 19, controls dispensation of pure water and water blank measurement by a photometric part 10. Next, the CPU dispenses reagents corresponding to test items into the reaction tubes 5 as first reagents, and samples into the reaction tubes 5 and B/F separation reaction tubes 15 of a B/F separation unit 7, and makes a sample suction dispensation mechanism 14 discharge solutions passing through the B/F separation process into the reaction tubes 5 to mix and stir 18 with second reagents. The CPU makes the photometric part 10 subject solutions resulting from dispensing first and second reagents or solutions discharged from the B/F separation unit 7 to photometry, displays the results on a CRT 11, and prints out 12.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an automatic analyzer for analyzing a sample such as serum.

[0002]

2. Description of the Related Art Generally, when a sample such as serum is analyzed, the analysis is performed by a biochemical analyzer or an immunoanalyzer depending on the analysis items, for example, biochemical analysis items and B / F separation required items. I was going.

[0003]

However, recently, the number of cases in which both the immunity item and the biochemistry item are measured for the same sample has increased, so that the immunoassay device and the biochemical analyzer are both provided. , Each had to set up a sample and perform the analysis. Therefore, it takes a lot of time to set the sample in the device, and a space for installing both devices is required.

The present invention has been made in order to solve the above-mentioned problems of the prior art, and the purpose thereof is to set both the immunity item and the biochemistry item once the sample is set in the apparatus. It is to provide an automatic analyzer capable of measuring.

[0005]

In order to achieve the above object, in the present invention, an immunoassay step which requires B / F separation for a sample supplied from a sample supply section,
It is characterized by comprising a measuring means capable of simultaneously performing the biochemical analysis step by the colorimetric method in correspondence with the measurement item.

[0006] Further, for the sample supplied from the sample supply section, an immunoturbidimetric analysis step using near infrared rays and a biochemical analysis step by a colorimetric method can be carried out at the same time depending on the measurement items. It is characterized by comprising means.

[0007] Further, for the sample supplied from the sample supply section, an immunoturbidimetric analysis step using near infrared rays,
It is characterized by comprising a measuring means capable of simultaneously carrying out the immunoassay step requiring the / F separation corresponding to the measurement items.

Further, the sample supplied from the sample supply section is subjected to an immunoassay step requiring B / F separation, an immunoturbidimetric analysis step using near infrared rays, and a biochemical analysis step by a colorimetric method. A measuring means that can be simultaneously performed corresponding to the measurement items is provided.

[0009]

In the automatic analyzer of the present invention having the above-mentioned structure, the immune unit is incorporated based on the automatic biochemical analyzer, so that the immunoanalyzer and the automatic biochemical analyzer can be integrated. Became. Therefore, once the sample is set in the device, both the immunity item and the biochemistry item can be measured.

[0010]

An embodiment of the present invention will be described below with reference to the drawings.

FIG. 1 is a perspective view showing the outline of an automatic analyzer according to an embodiment of the present invention. In the figure, the automatic analyzer comprises a sample section 2 in which a plurality of sample tubes 1 for receiving samples such as serum collected from a human body are arranged,
Reagent tube 3 containing reagents corresponding to test items for each type
And a reaction part 6 having a plurality of reaction tubes 5 for mixing and reacting a sample and a reagent. In addition, the automatic analyzer uses the B for performing B / F separation.
It has an / F separation unit 7 and an immunizing reagent section 8 containing an immunizing reagent. Further, the automatic analyzer includes an operation unit 9 for inputting information such as test items, a CRT 11 and a printer 12 for displaying the photometric result information measured by the photometric unit 10, and a system controller 13 for controlling the entire automatic analyzer. With.

The sample section 2 has a sample suction / dispensing mechanism 14 for sucking and dispensing the sample in the sample tube 1, and the sample suction / dispensing mechanism 14 is controlled under the control of the system controller 13. The sample tube 1 is selected, and a predetermined amount of the sample in the sample tube 1 is sucked, and then the predetermined reaction tube 5 of the reaction section 6 or the predetermined B / F separation reaction tube 15 of the B / F separation unit 7 is selected. It is configured to dispense.

The reagent section 4 has a reagent suction / dispensing mechanism 17 for sucking and dispensing the reagent in the reagent tube 3 corresponding to the input test item by the nozzle 16, and this reagent suction / dispensing mechanism 17 is provided. Under the control of the system controller 13, is configured so that a desired reagent can be sucked and then dispensed into the reaction tube 5.

The reaction section 6 is a stirring section 1 for stirring a solution in which the sample and the reagent dispensed in the reaction tube 5 are mixed.
8, a photometric section 10 for analyzing the agitated solution, a cleaning section 19 for cleaning the inside of the reaction tube 5 before and after the analysis, and a constant temperature of the solution in the reaction tube 5 so that a stable reaction can be carried out. And a constant temperature part 20 for

The B / F separation unit 7 includes a stirring unit (not shown) for stirring a solution in which the sample dispensed in the B / F separation reaction tube 15 and the immunizing reagent are mixed, and an analysis unit. A cleaning unit (not shown) for cleaning the inside / outside of the B / F separation reaction tube, a magnet (not shown) for performing B / F separation, which will be described later, and a probe setting plate 21 for setting each probe. have. In addition, the immunological reagent section 8
Is a first immunization reagent part 22 containing a first immunization reagent (magnetic substance-bound antibody) and a second immunization reagent (enzyme-labeled antibody (P
OD)) in the second immunity reagent section 23, and the first immunity reagent dispensing mechanism 24 and the second immunity reagent dispensing mechanism 25 are respectively controlled under the control of the system controller 13. After aspirating the first immunization reagent and the second immunization reagent, B
The / F separation reaction tube 15 can be dispensed, and the probe of each dispensing mechanism can be set by the probe setting plate 21.

The photometric section 10 transmits a light source 26 and light from the light source 26 to a solution in the reaction tube 5 in which the sample and the reagent are mixed, and disperses the transmitted light for each wavelength. 27 and a photodiode array 28 that receives the dispersed light and detects the absorption for each wavelength. For example, 340 nm to 808 nm and 950 nm.
It is a multi-wavelength spectrophotometry system that can receive the near infrared rays of the. Then, the absorbance of the solution is measured (water blank measurement), and this measurement data is used as the calibration reference data. Reaction tube 5
Since the soiling differs depending on the measurement, the calibration reference data is obtained each time before measuring the absorbance of the solution.

FIG. 2 is a block diagram showing the structure of the automatic analyzer. In the figure, the system controller 13
Is a CPU 29 and an operation unit 9 that control the overall operation of the device.
A memory 30 for storing the test item information inputted to the test item and reagent information corresponding to the test item information, and a program memory 31 for storing a series of operation command information for executing an analysis based on the test item as a program. And is configured. The system controller 13 is electrically connected to the operation unit 9, the CRT 11, the printer 12, the reagent suction / dispensing mechanism 17, the sample suction / dispensing mechanism 14, the reaction unit 6, and the B / F separation unit 7, and the reaction unit. In addition to the system controller 13, a reagent suction / dispensing mechanism 17 and a sample suction / dispensing mechanism 14 are electrically connected to the system 6.

Next, the overall operation of the automatic analyzer is shown in FIG.
This will be described with reference to the flowchart in FIG. When making measurements
The reagent and the sample may be dispensed into the reaction tube 5 in any order, but an example in which the reagent is dispensed first will be described. In addition, not only one type of reagent but also two or more types of reagent may be used for one test item as necessary. In the following description of operation, the case of using two types of reagents will be described.

First, when the power is turned on by pressing the start key (not shown) of the operation unit 9, the start signal is the CPU.
Then, the CPU 29 reads out the program stored in the program memory 31 and the preparation is completed (step ST1). Next, when information such as test items is input from the operation unit 9, the CPU 29 stores the test item information in the memory 30 and controls the cleaning unit 19 to clean the inside of the reaction tube 5 (step ST2). And CP
U29 controls the cleaning unit 19 to dispense pure water into the reaction tube 5, causes the photometric unit 10 to perform a water blank measurement, and discharges the pure water used for this measurement after this measurement is completed (step S
T3). The CPU 29 searches the test item information stored in the memory 30, controls the reagent aspirating / dispensing mechanism 17, and selects the reagent corresponding to the test item information input to the operation unit 9.
By sucking pure water as a suction and dispensing medium, the pure water is sucked into the nozzle 16 (step ST4). Under the control of the CPU 29, the reagent aspirating and dispensing mechanism 17 dispenses the previously aspirated reagent as the first reagent into the reaction tube 5 of the reaction unit 6 (step ST5).

Next, the CPU 29 causes the sample suction / dispensing mechanism 14 to suck the sample in the sample section 2, and dispenses the sucked sample into the B / F separation reaction tube 15 of the B / F separation unit 7 ( Step ST6), B / F described later
After the separation process (step ST7), this solution is sucked by the sample suction / dispensing mechanism 14 and discharged into the reaction tube 5 (step ST8).

On the other hand, the CPU 29 causes the sample suction / dispensing mechanism 14 to suck the sample in the sample section 2, and dispenses the sucked sample into the same reaction tube 5 dispensed in step ST5 (step ST9). Then, CPU
29 causes the stirring section 18 to stir the solution of the reagent and sample dispensed into the reaction tube 5 (step ST10). As in step ST9, the CPU 29 causes the reagent suction / dispensing mechanism 17 to operate.
Is controlled to dispense the reagent as the second reagent into the solution obtained in step 7 (step ST11), and the solution is stirred by the stirring unit 18 (step ST12).

Then, the CPU 29 causes the photometric unit 10 to perform photometry on the solution in which the first reagent and the second reagent have been dispensed or the solution discharged from the B / F separation unit 7, and receives the photometric result information (step ST13). ). The CPU 29 sends the received photometric result information to the CRT 11 for image display, and also sends the photometric result information to the printer 12 for print processing (step ST14). Cleaning section 1
Under the control of the CPU 29, 9 discharges the solution in the reaction tube 5 for which analysis has been completed, cleans this reaction tube 5 (step ST15), and completes the measurement (step ST1).
6).

Next, the B / F separation process in step ST7 will be described with reference to the position flow shown in FIG. The numbers shown in the figure mean the position numbers of the B / F separation reaction tube.

First, the sample containing the antigen to be measured is subjected to the B / F separation reaction tube 15 by the sample suction / dispensing mechanism 14.
(No. 1), followed by a B / F separation reaction in which the first immunizing reagent, which is a magnetic substance-bound antibody, is put into the sample from the first immunizing reagent part 22 by the first immunizing reagent dispensing mechanism Tube 1
Discharge to No. 5 (No. 2). As a result, the first immune reaction (antigen / antibody reaction) is performed. And by magnet B /
With the magnetic substance-bound antibody adsorbed at one place in the F separation reaction tube 15 (No. 13 to No. 15), the B / F separation reaction tube 15
The solution containing the released antigen in the inside is aspirated (No.15), then the washing solution (PBS) is discharged into the B / F separation reaction tube 15 (No.16), and the magnetic substance-bound antibody is transferred to the B / F by a magnet. In the state where it is adsorbed at one place in the separation reaction tube 15 (No. 17 to No. 1
9), the solution in the B / F separation reaction tube 15 is sucked and removed (No. 19), whereby B / F separation is performed.

Next, the second immunizing reagent, which is an enzyme-labeled antibody, is discharged from the second immunizing reagent section 23 to the B / F separation reaction tube 15 by the second immunizing reagent dispensing mechanism 25 (No. 20).
), By stirring with a stirring unit (No. 21),
A second immune reaction (antigen / antibody reaction) is performed. And
With the magnetic substance-bound antibody adsorbed at one place in the B / F separation reaction tube 15 by a magnet (No.35 to No.37), B /
The solution containing the released enzyme-labeled antibody in the F separation reaction tube 15 is suctioned and removed (No. 37).

The cleaning liquid was discharged into the B / F separation reaction tube 15 (No. 38), and this was stirred by the stirring section (No. 39).
), B / F separation reaction tube 1 using a magnet to bind the magnetic substance-bound antibody
While being adsorbed at one place in No. 5 (No.40 to No.42),
The solution in the B / F separation reaction tube 15 is sucked and removed (N
o.42). This No. 38 ~ No. 42 process is No. 43 ~ No. 47
Repeat No. 48 to No. 52 in the same manner, and repeat B
/ F separation is performed. Then, the substrate solution (TMBZ) is added to the B / F.
It is discharged into the separation reaction tube 15 (No. 53) and the third reaction (enzymatic reaction) is carried out.

After passing through such a B / F separation step, this solution is sucked by the sample suction / dispensing mechanism 14 and discharged into the reaction tube 5, and photometry is performed.

Further, although this B / F separation example is based on the magnet system, it is also applicable to the B / F separation based on the beads system, the membrane system and the like.

As described above, since the present apparatus is based on the automatic biochemical analysis apparatus and is equipped with the B / F separation unit, it is possible to integrate the immunological analysis apparatus and the automatic biochemical analysis apparatus. became. Therefore, once the sample is set in the device, both immunity items and biochemistry items can be measured, and the measurement time can be shortened. Also, the device space can be reduced. Furthermore, since all items are measured using the same photometric system, it is possible to reduce variations in measurement data.

[0030]

EFFECT OF THE INVENTION The automatic analyzer of the present invention has the above-mentioned structure and action. Once the sample is set in the device, both the immunological item and the biochemical item can be measured. The time can be shortened. Further, since the immunoanalyzer and the automatic biochemical analyzer are integrated, the device space can be reduced. Furthermore, since all items are measured using the same photometric system, it is possible to reduce variations in measurement data.

[Brief description of drawings]

FIG. 1 is a schematic diagram showing an outline of an automatic analyzer according to an embodiment of the present invention.

FIG. 2 is a block diagram showing a configuration of an automatic analyzer according to the same embodiment.

FIG. 3 is a flowchart showing the operation of the automatic analyzer in the same example.

FIG. 4 is a diagram showing an example of a position flow of a B / F separation unit of the automatic analyzer according to the same embodiment.

[Explanation of symbols]

 2 sample part (sample supply part) 4 reagent part 6 reaction part 7 B / F separation unit 8 immunological reagent part 10 photometric part 13 system controller

Claims (4)

[Claims] 1. A measurement capable of simultaneously performing an immunoassay step requiring B / F separation and a biochemical analysis step by a colorimetric method on a sample supplied from a sample supply section in correspondence with measurement items. An automatic analyzer characterized by comprising means. 2. A measurement capable of simultaneously performing an immunoturbidimetric analysis step using near-infrared rays and a biochemical analysis step by a colorimetric method on a sample supplied from a sample supply section in correspondence with measurement items. An automatic analyzer characterized by comprising means. 3. An immunoturbidimetric analysis step using near infrared rays for the sample supplied from the sample supply section, and B / F
An automatic analyzer characterized by comprising a measuring means capable of simultaneously performing an immunoassay step requiring separation according to measurement items. 4. The sample supplied from the sample supply unit is provided with an immunoassay step requiring B / F separation, an immunoturbidimetric analysis step using near infrared rays, and a biochemical analysis step by a colorimetric method. An automatic analyzer characterized in that it comprises a measuring means that can be carried out at the same time according to the measurement item.