JPH0588299U - Petri dish - Google Patents

Abstract

(57) [Summary] [Purpose] To impart hydrophilicity to the inner surface of a plastic petri dish so that it can be used for culturing microorganisms and cells. [Structure] In a plastic petri dish, a plastic film layer having silicon oxide vapor-deposited on the inner surface of the bottom is attached by an insert injection molding method.

Description

[Detailed description of the device]

[0001]

[Industrial applications]

 The present invention relates to a plastic petri dish suitable for culturing microorganisms and cells.

[0002]

[Prior Art]

 The conventional plastic petri dish has a property that it repels aqueous samples and culture medium because the inner surface has a surface tension of about 30-40 dyne / cm, which is hydrophobic. It was necessary to add fresh aqueous samples and medium. In order to solve this problem, Japanese Utility Model Publication No. 62-35628 discloses a petri dish in which a laminated sheet of a hydrophilic polyvinyl alcohol resin and a hydrophobic synthetic resin is formed by drawing a polyvinyl alcohol resin layer on the inner surface. Further, Japanese Utility Model Laid-Open No. 60-55400 discloses a method of coating quartz on the culture surface of a plastic container to make it hydrophilic.

[0003]

[Problems to be solved by the device]

 However, in the method described in Japanese Utility Model Laid-Open No. 62-35628, there is a case where the polyvinyl alcohol on the inner surface causes hydrolysis during use and the surface state of the culture surface becomes unstable. Further, the method according to Japanese Utility Model Laid-Open No. 60-55400 has a problem in that the quality is stabilized in practical use since it is coated in a later step to impart hydrophilicity. The inventors of the present invention focused on the fact that the surface of the glass was well wetted by an aqueous sample or a culture medium, and in consideration of the ease of handling when forming a petri dish, the plastic on which silicon oxide was deposited was deposited. I came up with the idea of using a film. The present invention uses the silicon oxide vapor-deposited plastic film to eliminate the above-mentioned instability during use of the culture surface and the need for post-treatment, and to provide a stable plastic dish with a hydrophilic surface. Is.

[0004]

Means for Solving the Problems The present invention, as shown in FIGS. 1 and 2, is a plastic petri dish and a plastic film (120) having a silicon oxide deposition layer (130) formed on the inner surface of the bottom. The petri dish (100) is characterized in that layers are attached by an insert injection molding method.

The above-described vapor-deposited silicon oxide is usually represented by a chemical formula of Si _x O _y (x = 1 or 2, y = 0 to 3), and is formed by vacuum vapor deposition, sputtering, ion plating, or the like. The thickness of the vapor-deposited film is preferably at least 100 Å or more.

Further, as the material of the plastic film on which silicon oxide is vapor-deposited, the thermoplastic resin of the petri dish main body and the thermoplastic resin capable of heat fusion during injection molding are generally used. When a plastic film that is not heat-sealed is used, an adhesive layer that thermally adheres to the thermoplastic resin of the petri dish body during injection molding is placed in advance on the back surface of the plastic film on which silicon oxide is deposited. ..

As shown in FIG. 2 (a), the method of depositing a plastic film (120) layer having silicon oxide vapor-deposited on it by insert injection molding is a male mold (210) of an injection molding die. At the site corresponding to the inner surface of the bottom of the petri dish, the silicon oxide vapor-deposited surface of the plastic film on which silicon oxide was vapor-deposited was fixed to the mold surface using vacuum suction or static electricity, and then shown in Figure 2 (b). As described above, it is melted by the injection molding device (300) into the cavity (240) which is the space between the male mold and the female mold in which the molded product of the injection-molded mold (200) that has been clamped is formed. A thermoplastic resin (350) is injected from a gate (230) provided in a female mold to mold a petri dish body, and at the same time, a plastic film layer deposited with silicon oxide is attached to the bottom inner surface of the petri dish. To do. As shown in FIG. 2C, the molded petri dish (100) is opened and discharged from the injection molding die.

Further, as the material of the petri dish body, a thermoplastic resin such as polystyrene, polypropylene, polycarbonate or polyester is used.

[0009]

[Action]

 The petri dish of the present invention comes into contact with an aqueous sample or medium necessary for culturing microorganisms or cells.Since the culture surface of the bottom inner surface of the petri dish is a silicon oxide deposition surface, it is hydrophilic and wets with the aqueous sample and medium. It is good and can create a good atmosphere for culture.

[0010]

【Example】

 FIG. 1 is a cross-sectional view of a petri dish of the present embodiment, and FIG. 2 is a cross-sectional view of a die showing the state of insert injection molding of the petri dish by steps. Next, an embodiment will be described with reference to the drawings.

First, on one surface of a 16 μm thick polyester film (material: polyethylene terephthalate), silicon oxide was vapor-deposited to a thickness of 800 Å by vacuum vapor deposition to prepare a vapor deposition film. The produced vapor deposition film was cut according to the shape of the inner surface of the bottom of the petri dish.

Next, as shown in FIG. 2A, a portion corresponding to the inner surface of the bottom of the petri dish of the male die (210) when the die for injection molding of the petri dish attached to the injection molding machine is opened. Then, the vapor deposition surface of the vapor deposition film was adsorbed and fixed on the surface of the mold by vacuum suction. Then, as shown in FIG. 2 (b), after the mold is clamped, it is melted by an injection molding device (300) into a cavity (240) formed by a male mold and a female mold (220). Molten polyethylene terephthalate (350) is injected from a gate (230) provided in a female mold to mold the petri dish body and at the same time, a plastic film (120) having a vapor deposition layer (130) of silicon oxide on the inner surface of the bottom is formed. The vapor-deposited surface was heat-bonded and stuck. The molded petri dish (100) of this example was taken out by opening the mold for injection molding, as shown in FIG. 2 (c).

Next, the prepared petri dish of this example was evaluated. The surface tension of the silicon oxide vapor-deposited surface on the inner surface of the bottom of the dish was 84 dyne / cm, and it was confirmed that the wet sample was well wetted with the aqueous sample and the medium. Furthermore, when the cells were actually cultivated using the petri dish of this Example, the petri dish of this Example had a very stable surface state, and created a good atmosphere for culturing the cells. It was

FIG. 3 shows the relationship between the deposition thickness of silicon oxide measured by the present inventor and the surface tension indicating the degree of wetting.

In this example, a vapor deposition film having a vapor deposition film thickness of silicon oxide of 800 Å was used. However, from the measurement results of FIG. 3, the surface tension of a vapor deposition film thickness of silicon oxide of 100 Å is 75 dyne / cm. In practice, if the surface tension is 75 dyne / cm or more, there is no problem. If the vapor-deposited film thickness of silicon oxide is 100 Å or more, it is considered that the sample or medium has sufficient wetting. Judged

[0016]

[Effect of the device]

 The petri dish of the present invention has a vapor-deposited layer of silicon oxide on the culturing surface, which is hydrophilic and wets well to create a good atmosphere for culturing microorganisms and cells, and the stable surface condition may cause reactions with microorganisms and cells. Absent. In addition, post-treatment for making the petri dish hydrophilic is unnecessary, and furthermore, sterilization treatment is easy and storage management is easy.

[Brief description of drawings]

FIG. 1 is a cross-sectional view of a petri dish of the present invention.

FIG. 2 is a cross-sectional view of a mold showing a process of insert injection molding of a petri dish of the present invention in each process.

FIG. 3 is a graph showing the relationship between the film thickness of vapor-deposited silicon oxide and the surface tension.

[Explanation of Codes] 100 ... Petri dish 110 ... Petri dish main body 120 ... Plastic film 130 ... Silicon oxide vapor deposition layer 200 ... Injection mold 210 ... Male 220 ... Female 230 ... Gate 240 ... … Cavity 300 …… Injection molding machine 350 …… Melted resin

Claims (1)

[Scope of utility model registration request] 1. A petri dish made of plastic, characterized in that a plastic film layer having silicon oxide vapor-deposited on the inner surface of the bottom is stuck by an insert injection molding method.