JPH0568562A - ヒトＣ型肝炎ウイルスのｃＤＮＡ、そのクローン及びこれらの利用法 - Google Patents

Abstract

(57)【要約】 【目的】 ヒト C 型肝炎ウイルスの cDNA、そのクロー
ン及びこれらの利用法を提供する。 【構成】 ヒト C 型肝炎確定診断患者 5 名由来の RNA
を鋳型として cDNA を作成し、PCR 法を利用して増幅
させ、PCR 産物をクローニングし、患者 1 名当り 3 ク
ローン、計 15 クローンを得、各クローンにおける塩基
配列及びアミノ酸配列を決定する。これらの 15 クロー
ンの内の 1 クローンは約9500 個のヌクレオチドを有
し、他のクローンは 5'-側翻訳不可能領域及びこれに続
く翻訳可能領域であって約 1800 個のヌクレオチドを有
している。これらクローン間の配列を比較検討して変異
の程度を知ると共に、共通している領域を見い出し、こ
れらの内の幾つかの領域をプローブ又は抗原として用い
ることによりヒト C 型肝炎ウイルスの検出やヒト C 型
肝炎の診断を行う。

Description

【発明の詳細な説明】

【０００１】

【産業上の利用分野】本発明はヒト C 型肝炎ウイルス
(以下「HCV」と略記する) の cDNA、そのクローン及び
これらの利用法に係る。

【０００２】

【従来の技術】ヒト C 型肝炎 [従来「非 A 非 B(NANB)
型肝炎」と称されていたもの] は輸血に起因する肝炎
の 90 - 95% を占めており、治療法も未だ確立するに至
っていないために、その診断法、治療薬及び予防用のワ
クチンの早期開発が強く要望されている。

【０００３】近年に至り、米国のカイロン社がチンパン
ジーの肝臓由来の cDNA をクローン化し、これをベクタ
ー (λgt11) に組込み、大腸菌で発現させたところ、そ
の産物が NANB 型肝炎患者の血清と反応することを見い
出している。最終的には、これが約 9000 個のヌクレオ
チドを有する一本鎖 RNA ウイルスであり、フラビウイ
ルスに近いウイルスであることを突き止め、これを HCV
と命名すると共に、その内で約 7000 個のヌクレオチ
ドからなる cDNA 塩基配列を公表した [EP 318216
(A)]。一方、日本人の NANB 型肝炎患者から得られた H
CV の塩基配列については、これまでに岡本等 ["The Ja
pan journal of Experimental Medicine", Vol. 60, pa
ges 167 - 177 (1990)]、加藤等 ["Proceedings ofthe
National Academy of Sciences of the U.S.A.", Vol.
87, pages 9524 - 9528 (1990)] の報告がある。 尚、
金子等は、日本人に関して HCV の一部の非構造領域に
おける多様性が個体間、更には同一個体内においても存
在することを報告している ("The 1990International S
ymposium on Viral Hepatitis and Liver Disease", 19
90 inpress)。

【０００４】上記のカイロン社は、HCV 遺伝子の一部を
用い酵母で発現させたポリペプチドを抗原として、それ
に反応する NANB 型肝炎患者血清中の抗体を検出するキ
ットを開発した。このキットは NANB 型肝炎患者が HCV
感染をしたか否かを判定するものであり、疫学的には
約 70% の確率で判定できる旨公表されている。この診
断法は HCV 抗原を検出するものではないため、抗体産
生が見られない感染初期の患者では感染しているか否か
の判定はできない。

【０００５】

【発明が解決しようとする課題】HCV 遺伝子の構造に関
する既述の従来技術において、カイロン社が報告してい
るのものはチンパンジー由来のものであり、岡本等の報
告は約 1900 個のヌクレオチドからなる部分配列を明ら
かにしているに過ぎない。一方、加藤等の報告は約 950
0 個のヌクレオチドからなるものであり、HCV 遺伝子の
ほぼ全長をカバーしているものと考えられるが、数人の
NANB 型肝炎患者のプール血清から RNAを抽出し、これ
を用いて cDNA を作成しており、従って単一のクローン
に関するものではない。

【０００６】上記の点と、金子等が指摘している既述の
多様性とを考慮に入れると、複数のNANB 患者由来の HC
V 遺伝子をクローン化すると共に、同一の患者由来の複
数の HCV 遺伝子をクローン化し、各クローンについて
塩基配列やアミノ酸配列を調べ、HCV 遺伝子の変異の程
度を比較検討することにより HCV 遺伝子において良く
保存されている共通の塩基配列領域やアミノ酸配列を見
い出すことが C 型肝炎に対する的確な診断法、治療
法、輸血用血液の処理法等を提供し、又ワクチンの開発
をもたらす上で肝要であると考えられるが、このような
研究報告はなされていない。従って、本発明の課題は複
数の患者に由来の RNA を鋳型として cDNA を作成し、
最近米国のシータス社により開発されたポリメラーゼ連
鎖反応 [PolymeraseChain Reaction (PCR)] 法を利用し
て増幅させ、PCR 産物をクローニングすることにより患
者一人当りから複数個の cDNA を確立すること並びにそ
の各 cDNAクローンの塩基配列やアミノ酸配列を決定
し、これらを比較検討して共通領域を見い出すことにあ
る。

【０００７】

【課題を解決するための手段及び作用】本発明者等は、
先ず、確定診断された5 名の NANB 型肝炎患者由来の肝
臓組織から RNA を分離精製し、この精製 RNA を鋳型と
して自体周知の手法で cDNAを作成した。この cDNA を
PCR 法を利用して増幅させ、PCR 産物をプラスミドでク
ローニングすることにより患者 1 名当り 3 種類の単一
クローン、計 15 クローンを確立させた。これらのクロ
ーンはそれぞれ後記の配列表における配列番号 1 - 15
に示される塩基配列及びアミノ酸配列を有していた。

【０００８】尚、上記の 15 クローンの内の 1 クロー
ン (配列番号 2, JK1-B) は HCV 遺伝子の全塩基配列を
含み、約 9500 個のヌクレオチドからなり、ヒト由来の
ものであって 一人の NANB 型肝炎患者から得られたも
のとしては初めてのクローン化成功例である。

【０００９】上記の NANB 型肝炎患者由来の HCV にお
けるそれぞれ単一の 15 クローン間での塩基配列を比較
検討した処、1734 塩基中で 1436 塩基が共通であり、2
5 塩基以上にわたって共通している領域は 9 箇所存在
していた。

【００１０】

【発明の効果】本発明によれば、HCV 遺伝子をコードす
る塩基配列を有する一本鎖 DNA 及び該一本鎖 DNA とそ
の相補一本鎖 DNA からなる二本鎖 DNA が提供される。
上記の一本鎖 DNA はこれをプローブとして用いること
により遺伝子レベルでの C 型肝炎の診断に利用するこ
とができ、又二本鎖 DNA は制限酵素により適宣の鎖長
を有するものとなして構造と生理活性との相関究明に利
用することができる。上記の一本鎖 DNA はクローン化
されていることができ、15 クローン存在する。従っ
て、その 15 クローン間で共通する塩基配列を有する領
域をプライマーとして選択し、このプライマーを用いて
PCR 法を実施すれば、高い精度を以ってC 型肝炎の診
断を行うことができる。

【００１１】尚、二本鎖ＤＮＡ断片の組み込まれるべき
ベクターに発現ベクターを選択し、本発明による遺伝子
を有している組換えベクターを作成し、このベクターを
大腸菌、酵母等の微生物や動物細胞に取り込ませ、該微
生物または動物細胞を培養すれば、HCV に起因する抗原
物質を大量に生産することができる。この場合抗原とし
て選択される領域は、本発明による 15 クローン間でよ
く保存されている部分、即ち共通している部分を選択す
ることが肝要である。従って、この産物を用いて酵素免
疫法、血球凝集法、ラテックス凝集法等を実施すれば、
高い精度を以って患者の血液中に存在する抗 HCV 抗体
量の測定が可能になり、HCV に起因する肝炎、癌等の進
行程度、回復の程度を診断することが可能となり、又キ
ャリヤーの診断も可能となる。尚、上記の抗原物質を用
いて、継代免疫等の手法により、弱毒化させることによ
りワクチン開発の途が開かれ、又上記の産物を動物に免
疫させることにより、抗体性物質を生産させ、これを取
得すれば治療薬や輸血用血液の処理剤を提供することが
でき、該抗体性物質をモノクローン、ポリクローン化す
れば治療薬としての安定供給が可能となる。

【００１２】

【実施例】次に、実施例に関して本発明を更に詳細に且
つ具体的に説明する。

【００１３】実施例 1 (A) NANB 型肝炎患者からの RNA の分離精製と相補 DNA
の合成 NANB 型肝炎患者 5 名からの RNA の分離精製は特願平
1 - 163715 明細書に記載の方法に従って行った。これ
により得られた精製 RNA に対して、プライマーとして
岡本等が "The Japan jounal of Experimental Medicin
e", Vol. 60, pages 167 - 177 (1990) において報告し
ている HCV 塩基配列を元に作成したプライマーを用い
逆転写酵素により cDNA を合成した。更に、上記の患者
5名の内の 1 名から得られた RNA に対してカイロン社
が EP 318216(A) において報告している HCV 塩基配列
を元に作成したプライマーを用い上記と同様に逆転写酵
素により cDNA を合成した。

【００１４】(B) PCR 法による cDNA の増幅とクローニ
ング 上記の (A) で得た 5 名の患者の cDNA をそれぞれ鋳型
として、先ず 5'-末端プライマーと (A) で用いた岡本
等のプライマーにより約 1900 個のヌクレオチドを PCR
法を利用して増幅させ、得られた PCR 産物を M13 プ
ラスミドにクローニングした。得られたクローンの内で
患者 1 名当たり３クローンを選び出し、計 15クローン
を得た。これらの各クローンは約 1900 個のヌクレオチ
ドを含んでいた。次に上記の (A) の末尾において述べ
た cDNA、即ち 患者 5 名の内の1 名から得られた RNA
を基にして合成された cDNA を鋳型として、上記の岡本
等及びカイロン社が報告している HCV 塩基配列を元に
作成したプライマーを用い、PCR 法を利用して目的の領
域を増幅させた。この場合にプライマーとしては、互い
に一部がオーバーラップするように 12 組を選んだ。得
られた PCR 産物を M13 プラスミドにクローニングし
た。このようにして得られた計 12 組の各組のクローン
からそれぞれ 1 クローン宛選択し、計 12 クローンを
得た。

【００１５】(C) 塩基配列の決定 上記の (B) で得たクローンの塩基配列をダイデオキシ
法により決定した。その結果は後記の配列表の配列番号
1 - 15 に示されている。

【００１６】実施例 2 (A) 患者間で保存された HCV 由来 DNA クローンの領域
を用いた遺伝子診断 NANB 型肝炎患者の血清から、例えばグアニジンチオシ
アネートを用いたチルグーイン等の方法 ["Biochemistr
y", Vol. 18, pages 5294 - 5299 (1979)] によって全
RNA を抽出した。この RNA に後記の配列表に示した配
列の一部 (配列番号 1 における 12 番目から 36 番目
迄の配列と、570 番目から 594 番目迄の相補鎖の配
列）をプライマーとして用い、逆転写酵素の存在下に 4
2℃ で 1 時間インキュベーションすることにより相補
DNA を合成した。この相補 DNA を用い、金子等の方法
["Journal of Clinical Microbiology", Vol. 27, page
s1930 - 1933 (1989)] に従って PCR を 2回行うことに
より DNA の大量増幅を行い、電気泳動によって予想さ
れた約 570 塩基対の長さのバンドを確認した。尚、 2
回目の PCR には配列表に示した配列番号 1 における 1
9 番目から 43番目迄の配列と、565 番目から 589 番目
迄の相補鎖の配列をプライマーとして用いた (上記 4
種類のプライマーをプライマーセット A とする)。更
に、別の部分のプライマー (1 回目には配列表の配列番
号 1 における 12 番目から 36番目迄の配列と 310 番
目から 334 番目迄の相補鎖の配列を用い、2 回目には
配列表の配列番号 1 の 19 番目から 43 番目までの配
列と、304 番目から 328 番目迄の相補鎖の配列を用い
る) で上記のように PCR を行うことによっても、予想
された約 310 塩基対の長さのバンドを電気泳動によっ
て確認することができた (この 4 種類のプライマーを
プライマーセット B とする)。

【００１７】この電気泳動の結果は図 1 に示されてい
る。図 1 に見られるように NANB 型肝炎患者の血清で
は予想された位置に PCR 産物由来のバンドが確認さ
れ、一方健常人の血清ではバンドは全く検出されなかっ
た。

【００１８】

【配列表】

配列番号：１ 配列の長さ：１７３４ 配列の型：核酸 鎖の数：二本鎖 トポロジー：直鎖状 配列の種類：cDNA to genomic RNA ハイポセティカル：Ｎｏ アンチセンス：Ｎｏ 起源 生物名：Hepatitis C Virus 単離クローン名：JK1-A 組織の種類：肝臓細胞 GGCGACACTC CACCATAGAT CACTCCCCTG TGAGGAACTG CTGTCTTCAC GACGAAAGCG 60 ＴＣＴＡＧＣＣＡＴＧ ＧＣＧＴＴＡＧＴＡＴ ＧＡＣＴＧＴＣＧＴＧ ＣＡＧ
ＣＣＴＧＣＡＧ ＧＡＣＣＣＣＣＣＣＴ ＣＣＣＧＧＧＡＧＡＧ 120 CCATAGTGGT CTGCGGAACC GGTGAGTACA CCGGAATTGC CAGGACGACC GGGTCCTTTC 180 TTGGATCACC CCGCTCAATG CCTGGAGATT TGGGCGTGCC CCCGCGAGAC TGCTAGCCGA 240 GTAGTGTTGG GTCGCGAAAG GCCTTGTGGT ACTGCCTGAT AGGGTGCTTG CGAGTGCCCC 300 GGGAGGTCTC GTAGACCGTG CACC ATG AGC ACA AAT CCC AAA CCT CAA 348 Met Ser Thr Asn Pro Lys Pro Gln 1 5 AGA AAA ACC AAA CGT AAC ACC AAC CGC CGC CCA CAG GAC GTC AAG TTC 396 Arg Lys Thr Lys Arg Asn Thr Asn Arg Arg Pro Gln Asp Val Lys Phe 10 15 20 CCG GGC GGT GGT CAG ATC GTT GGT GGA GTT TAC CTG TTG CCG CGC AGG 444 Pro Gly Gly Gly Gln Ile Val Gly Gly Val Tyr Leu Leu Pro Arg Arg 25 30 35 40 GGC CCC AGG TTG GGT GTG CGC GCG ACT AGG AAG ACT TCC GAG CGG TCG 492 Gly Pro Arg Leu Gly Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Ser 45 50 55 CAA CCT CGT GGA AGG CGA CAA CCT ATC CCC AAG GCT CGC CAG CCC GAG 540 Gln Pro Arg Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Gln Pro Glu 60 65 70 GGC AGG GCC TGG GCT CAG CCC GGG TAC CCT TGG CCC CTC TAT GGC AAT 588 Gly Arg Ala Trp Ala Gln Pro Gly Tyr Pro Trp Pro Leu Tyr Gly Asn 75 80 85 GAG GGT CTG GGG TGG GCA GGA TGG CTC CTG TCA CCC TAC GGC TCC CGG 636 Glu Gly Leu Gly Trp Ala Gly Trp Leu Leu Ser Pro Tyr Gly Ser Arg 90 95 100 CCT AGG TGG GGC CCC ACG GAC CCC CGG CGT AGG TCG CGT AAT TTG GGT 684 Pro Arg Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Asn Leu Gly 105 110 115 120 AAG GTC ATC GAT ACC CTC ACA TGC GGC TTC GCC GAC CTC ATG GGG TAC 732 Lys Val Ile Asp Thr Leu Thr Cys Gly Phe Ala Asp Leu Met Gly Tyr 125 130 135 ATT CCG CTC GTC GGC GCC CCC CTA GGA GGC GCT GCC AGG GCC CTG GCG 780 Ile Pro Leu Val Gly Ala Pro Leu Gly Gly Ala Ala Arg Ala Leu Ala 140 145 150 CAT GGC GTC CGG GTT CTG GAG GAC GGC GTG AAC TAC GCA ACA GGG AAT 828 His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 155 160 165 CTG CCC GGT TGC TCT TTT TCT ATC TTC CTC TTG GCC TTG CTG TCC TGT 876 Leu Pro Gly Cys Ser Phe Ser Ile Phe Leu Leu Ala Leu Leu Ser Cys 170 175 180 CTG ACC GTC CCA GTT TCC ACT TAT GAA GTG CGC AAC GTG TCC GGG GTG 924 Leu Thr Val Pro Val Ser Thr Tyr Glu Val Arg Asn Val Ser Gly Val 185 190 195 200 TAC CAT GTC ACG AAC GAC TGC TCC AAC TCA AGT ATT GTG TAT GAG GCA 972 Tyr His Val Thr Asn Asp Cys Ser Asn Ser Ser Ile Val Tyr Glu Ala 205 210 215 GCG GAC ATG ATT ATG CAT ACC CCC GGG TGC GTA CCC TGC GTC CGG GAG 1020 Ala Asp Met Ile Met His Thr Pro Gly Cys Val Pro Cys Val Arg Glu 220 225 230 GGC AAT TCC TCC TGC TGC TGG GTA GCG CTC ACT CCC ACG CTC GCG GCC 1068 Gly Asn Ser Ser Cys Cys Trp Val Ala Leu Thr Pro Thr Leu Ala Ala 235 240 245 AGG AAC AGC AGC ATC CCC ACT ACG ACA ATA CGA CGA CAT GTC GAT TTG 1116 Arg Asn Ser Ser Ile Pro Thr Thr Thr Ile Arg Arg His Val Asp Leu 250 255 260 CTC GTT GGG GCA GCT GCT CTC TGC TCC GCT ATG TAC GTG GGG GAT CTC 1164 Leu Val Gly Ala Ala Ala Leu Cys Ser Ala Met Tyr Val Gly Asp Leu 265 270 275 280 TGT GGG TCT GTT TTC CTC GTC TCC CAA TTG TTC ACC TTC TCA CCT CGC 1212 Cys Gly Ser Val Phe Leu Val Ser Gln Leu Phe Thr Phe Ser Pro Arg 285 290 295 CGG TAT GAG ACG GTA CAG GAC TGC AAT TGC TCA CTC TAT CCC GGC CAC 1260 Arg Tyr Glu Thr Val Gln Asp Cys Asn Cys Ser Leu Tyr Pro Gly His 300 305 310 GTA TCA GGT CAT CGT ATG GCT TGG GAT ATG ATG ATG AAC TGG TCA CCT 1308 Val Ser Gly His Arg Met Ala Trp Asp Met Met Met Asn Trp Ser Pro 315 320 325 ACA ACA GCC TTA GTG GTG TCG CAG TTA CTC CGG ATC CCA CAA GCC ATC 1356 Thr Thr Ala Leu Val Val Ser Gln Leu Leu Arg Ile Pro Gln Ala Ile 330 335 340 GTG GAT ATG GTG GTG GGG GCC CAC TGG GGA GTC CTG GCG GGC CTC GCC 1404 Val Asp Met Val Val Gly Ala His Trp Gly Val Leu Ala Gly Leu Ala 345 350 355 360 TAC TAT TCC ATG GTG GGG AAC TGG GCT AAG GTC TTG ATT GTG ATG CTA 1452 Tyr Tyr Ser Met Val Gly Asn Trp Ala Lys Val Leu Ile Val Met Leu 365 370 375 CTC TTT GCC GGC GTT GAC GGG ACT ACC TAC GTG TCA GTG GGG CAT GCA 1500 Leu Phe Ala Gly Val Asp Gly Thr Thr Tyr Val Ser Val Gly His Ala 380 385 390 AGC CGG ACC ACC CAG CGC GTT GCG TCC TTC TTC AGT CCC GGC TCG GCT 1548 Ser Arg Thr Thr Gln Arg Val Ala Ser Phe Phe Ser Pro Gly Ser Ala 395 400 405 CAG AAA ATC CAA CTT GTT AAC ACC AAC GGC AGT TGG CAC ATC AAC AGG 1596 Gln Lys Ile Gln Leu Val Asn Thr Asn Gly Ser Trp His Ile Asn Arg 410 415 420 ACT GCC CTG AAT TGC AAC GAG TCC ATC AAC ACT GGG TTC TTT GCG GCG 1644 Thr Ala Leu Asn Cys Asn Glu Ser Ile Asn Thr Gly Phe Phe Ala Ala 425 430 435 440 CTG TTT TAC GCA AAG AAG TTC AAC TCG TCT GGA TGC TCA GAG CGC ATG 1692 Leu Phe Tyr Ala Lys Lys Phe Asn Ser Ser Gly Cys Ser Glu Arg Met 445 450 455 GCT AGC TGT CGT CCC ATT GAC AAA TTC GCT CAA GGG TGG GGT 1734 Ala Ser Cys Arg Pro Ile Asp Lys Phe Ala Gln Gly Trp Gly 460 465 470 配列番号：２ 配列の長さ：９４０５ 配列の型：核酸 鎖の数：二本鎖 トポロジー：直鎖状 配列の種類：cDNA to genomic RNA ハイポセティカル：Ｎｏ アンチセンス：Ｎｏ 起源 生物名：Hepatitis C Virus 単離クローン名：JK1-B 組織の種類：肝臓細胞 GGCGACACTC CACCATAGAT CACTCCCCTG TGAGGAACTG CTGTCTTCAC GACGAAAGCG 60 TCTAGCCATG GCGTTAGTAT GACTGTCGTG CAGCCTGCAG GACCCCCCCT CCCGGGAGAG 120 CCATAGTGGT CTGCGGAACC GGTGAGTACA CCGGAATTGC CAGGACGACC GGGTCCTTTC 180 TTGGATCACC CCGCTCAATG CCTGGAGATT TGGGCGTGCC CCCGCGAGAC TGCTAGCCGA 240 GTAGTGTTGG GTCGCGAAAG GCCTTGTGGT ACTGCCTGAT AGGGTGCTTG CGAGTGCCCC 300 GGGAGGTCTC GTAGACCGTG CACC ATG AGC ACA AAT CCT AAA CCT CAA 348 Met Ser Thr Asn Pro Lys Pro Gln 1 5 AGA AAA ACC AAA CGT AAC ACC AAC CGC CGC CCA CAG GAC GTC AAG TTC 396 Arg Lys Thr Lys Arg Asn Thr Asn Arg Arg Pro Gln Asp Val Lys Phe 10 15 20 CCG GGC GGT GGT CAG ATC GTT GGT GGA GTT TAC CTG TTG CCG CGC AGG 444 Pro Gly Gly Gly Gln Ile Val Gly Gly Val Tyr Leu Leu Pro Arg Arg 25 30 35 40 GGC CCC AGG TTG GGT GTG CGC GCG ACC AGG AAG ACT TCC GAG CGG TCG 492 Gly Pro Arg Leu Gly Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Ser 45 50 55 CAA CCT CGT GGA AGG CGA CAA CCT ATC CCC AAG GCT CGC CAG CCC GAG 540 Gln Pro Arg Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Gln Pro Glu 60 65 70 GGC AGG GCC TGG GCT CAG CCC GGG TAC CCT TGG CCC CTC TAT GGC AAT 588 Gly Arg Ala Trp Ala Gln Pro Gly Tyr Pro Trp Pro Leu Tyr Gly Asn 75 80 85 GAG GGT CTG GGG TGG GCA GGA TGG CTC CTG TCA CCC TAC GGC TCC CGG 636 Glu Gly Leu Gly Trp Ala Gly Trp Leu Leu Ser Pro Tyr Gly Ser Arg 90 95 100 CCT AGG TGG GGC CCC ACG GAC CCC CGG CGT AGG TCG CGT AAT TTG GGT 684 Pro Arg Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Asn Leu Gly 105 110 115 120 AAG GTC ATC GAT ACC CTC ACA TGC GGC TTC GCC GAC CTC ATG GGG TAC 732 Lys Val Ile Asp Thr Leu Thr Cys Gly Phe Ala Asp Leu Met Gly Tyr 125 130 135 ATT CCG CTC GTC GGG GCC CCC CTA GGA GGC GCT GCC AGG GCC CTG GCG 780 Ile Pro Leu Val Gly Ala Pro Leu Gly Gly Ala Ala Arg Ala Leu Ala 140 145 150 CAT GGC GTC CGG GTT CTG GAG GAC GGC GTG AAC TAC GCA ACA GGG AAT 828 His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 155 160 165 CTG CCC GGT TGC TCT TTT TCT ATC TTC CTC TTG GCC TTG CTG TCC TGT 876 Leu Pro Gly Cys Ser Phe Ser Ile Phe Leu Leu Ala Leu Leu Ser Cys 170 175 180 CTG ACC GTC CCA GTT TCC ACT TAT GAA GTG CGC AAC GTG TCC GGG GTG 924 Leu Thr Val Pro Val Ser Thr Tyr Glu Val Arg Asn Val Ser Gly Val 185 190 195 200 TAC CAT GTC ACG AAC GAC TGC TCC AAC TCA AGT ATT GTG TAT GAG GCA 972 Tyr His Val Thr Asn Asp Cys Ser Asn Ser Ser Ile Val Tyr Glu Ala 205 210 215 GCG GAC ATG ATT ATG CAT ACC CCC GGG TGC GTA CCC TGC GTC CGG GAG 1020 Ala Asp Met Ile Met His Thr Pro Gly Cys Val Pro Cys Val Arg Glu 220 225 230 GGT AAT TCC TCC CGC TGC TGG GTA GCG CTC ACT CCC ACG CTC GCG GCC 1068 Gly Asn Ser Ser Arg Cys Trp Val Ala Leu Thr Pro Thr Leu Ala Ala 235 240 245 AGG AAC AGC AGC ATC CCC ACT ACG ACA ATA CGA CGA CAT GTC GAT TTG 1116 Arg Asn Ser Ser Ile Pro Thr Thr Thr Ile Arg Arg His Val Asp Leu 250 255 260 CTC GTT GGG GCA GCT GCT CTC TGC TCC GCT ATG TAC GTG GGG GAT CTC 1164 Leu Val Gly Ala Ala Ala Leu Cys Ser Ala Met Tyr Val Gly Asp Leu 265 270 275 280 TGT GGG TCT GTT TTC CTC GTC TCC CAA TTG TTC ACC TTC TCA CCT CGC 1212 Cys Gly Ser Val Phe Leu Val Ser Gln Leu Phe Thr Phe Ser Pro Arg 285 290 295 CGG TAT GAG ACG GTA CAG GAC TGC AAT TGC TCA CTC TAT CCC GGC CAC 1260 Arg Tyr Glu Thr Val Gln Asp Cys Asn Cys Ser Leu Tyr Pro Gly His 300 305 310 GTA TCA GGT CAC CGT ATG GCT TGG GAT ATG ATG ATG AAC TGG TCA CCT 1308 Val Ser Gly His Arg Met Ala Trp Asp Met Met Met Asn Trp Ser Pro 315 320 325 ACA ACA GCC TTA GTG GTG TCG CAG TTA CTC CGG ATC CCA CAA GCC GTC 1356 Thr Thr Ala Leu Val Val Ser Gln Leu Leu Arg Ile Pro Gln Ala Val 330 335 340 GTG GAC ATG GTG GTG GGG GCC CAC TGG GGA GTC CTG GCG GGC CTC GCC 1404 Val Asp Met Val Val Gly Ala His Trp Gly Val Leu Ala Gly Leu Ala 345 350 355 360 TAC TAT TCC ATG GTG GGG AAC TGG GCT AAG GTC TTG ATT GTG ATG CTA 1452 Tyr Tyr Ser Met Val Gly Asn Trp Ala Lys Val Leu Ile Val Met Leu 365 370 375 CTC TTT GCC GGC GTT GAC GGG ACT ACC TAC GTG TCA GTG GGG CAT GCA 1500 Leu Phe Ala Gly Val Asp Gly Thr Thr Tyr Val Ser Val Gly His Ala 380 385 390 AGC CAG ACC ACC CGG CGC GTT GCG TCC TTC TTC AGT CCT GGC TCG GCT 1548 Ser Gln Thr Thr Arg Arg Val Ala Ser Phe Phe Ser Pro Gly Ser Ala 395 400 405 CAG AAA ATC CAA CTT GTT AAC ACC AAC GGC AGT TGG CAC ATC AAC AGG 1596 Gln Lys Ile Gln Leu Val Asn Thr Asn Gly Ser Trp His Ile Asn Arg 410 415 420 ACT GCC CTG AAT TGC AAC GAG TCC ATC AAC ACT GGG TTC TTT GCC GCG 1644 Thr Ala Leu Asn Cys Asn Glu Ser Ile Asn Thr Gly Phe Phe Ala Ala 425 430 435 440 CTG TTC TAC GTA AAG AAG TTC AAC TCG TCT GGA TGC TCA GAG CGC ATG 1692 Leu Phe Tyr Val Lys Lys Phe Asn Ser Ser Gly Cys Ser Glu Arg Met 445 450 455 GCT AGC TGT CGT CCC ATT GAC AGG TTC GCT CAA GGG TGG GGT CCC ATC 1740 Ala Ser Cys Arg Pro Ile Asp Arg Phe Ala Gln Gly Trp Gly Pro Ile 460 465 470 ACC CAT GCT GAG TCT CGC AGC TCG GAC CAA AGG CCT TAC TGC TGG CAC 1788 Thr His Ala Glu Ser Arg Ser Ser Asp Gln Arg Pro Tyr Cys Trp His 475 480 485 TAC GCA CCT CAA CCG TGT GGT ATC GTG CCC GCG TTG CAG GTG TGC GGT 1836 Tyr Ala Pro Gln Pro Cys Gly Ile Val Pro Ala Leu Gln Val Cys Gly 490 495 500 CCA GTG TAT TGT TTC ACC CCA AGC CCT GTT GTA GTG GGG ACG ACC GAC 1884 Pro Val Tyr Cys Phe Thr Pro Ser Pro Val Val Val Gly Thr Thr Asp 505 510 515 520 CGT TTC GGC GTC CCC ACG TAT AAC TGG GGG GAC AAT GAG ACG GAC GTG 1932 Arg Phe Gly Val Pro Thr Tyr Asn Trp Gly Asp Asn Glu Thr Asp Val 525 530 535 CTA CTC CTC AAC AAC ACG CGG CCG CCG CAA GGA AAC TGG TTC GGC TGT 1980 Leu Leu Leu Asn Asn Thr Arg Pro Pro Gln Gly Asn Trp Phe Gly Cys 540 545 550 ACA TGG ATG AAC AGC ACC GGG TTC ACC AAG ACG TGC GGG GGC CCC CCG 2028 Thr Trp Met Asn Ser Thr Gly Phe Thr Lys Thr Cys Gly Gly Pro Pro 555 560 565 TGT AAT ATC GGG GGG GCC GGC AAC AAC ACC TTG ACC TGC CCC ACG GAT 2076 Cys Asn Ile Gly Gly Ala Gly Asn Asn Thr Leu Thr Cys Pro Thr Asp 570 575 580 TGC TTC CGG AAG CAC CCC GAG GCC ACT TAC ACC AAG TGC GGC TCG GGG 2124 Cys Phe Arg Lys His Pro Glu Ala Thr Tyr Thr Lys Cys Gly Ser Gly 585 590 595 600 CCT TGG TTG ACA CCT AGG TGC ATG GTT GAC TAC CCA TAC AGA CTT TGG 2172 Pro Trp Leu Thr Pro Arg Cys Met Val Asp Tyr Pro Tyr Arg Leu Trp 605 610 615 CAC TAC CCC TGC ACT TTC AAC TTT ACC ATC TTT AAG ATC AGG ATG TAT 2220 His Tyr Pro Cys Thr Phe Asn Phe Thr Ile Phe Lys Ile Arg Met Tyr 620 625 630 GTG GGG GGT GTG GAG CAC AGG CTC AAC GCA GCG TGC AAC TGG ACT CGA 2268 Val Gly Gly Val Glu His Arg Leu Asn Ala Ala Cys Asn Trp Thr Arg 635 640 645 GGA GAG CGT TGT AAC ATA GAG GAC AGG GAT AGG TCA GAG CTT AGC CCG 2316 Gly Glu Arg Cys Asn Ile Glu Asp Arg Asp Arg Ser Glu Leu Ser Pro 650 655 660 CTG CTA CTG TCT ACA ACG GAG TGG CAG ATA CTA CCC TGT TCC TTT ACC 2364 Leu Leu Leu Ser Thr Thr Glu Trp Gln Ile Leu Pro Cys Ser Phe Thr 665 670 675 680 ACC CTA CCA GCT CTG TCC ACT GGT TTG ATC CAC CTC CAT CAG AAC ATC 2412 Thr Leu Pro Ala Leu Ser Thr Gly Leu Ile His Leu His Gln Asn Ile 685 690 695 GTG GAC GTG CAA TAC CTG TAC GGT GTA GGG TCA GCA GTT GTC TCC ATT 2460 Val Asp Val Gln Tyr Leu Tyr Gly Val Gly Ser Ala Val Val Ser Ile 700 705 710 GTG ATC AAA TGG GAG TAC GTT CTG CTG CTC TTC CTC CTC CTG GCG GAC 2508 Val Ile Lys Trp Glu Tyr Val Leu Leu Leu Phe Leu Leu Leu Ala Asp 715 720 725 GCG CGC GTC TGC GCC TGC TTA TGG ATG ATG CTG CTG ATA GCC CAG GCT 2556 Ala Arg Val Cys Ala Cys Leu Trp Met Met Leu Leu Ile Ala Gln Ala 730 735 740 GAG GCC GCC TTA GAA AAC CTG GTG GTC CTC AAT GCG GCG TCC GTG GCC 2604 Glu Ala Ala Leu Glu Asn Leu Val Val Leu Asn Ala Ala Ser Val Ala 745 750 755 760 GGA GCG CAT GGC ATC CTC TCA TTT CTT GTG TTC TTC TGT GCA GCC TGG 2652 Gly Ala His Gly Ile Leu Ser Phe Leu Val Phe Phe Cys Ala Ala Trp 765 770 775 TAC ATC AAG GGC AGG CTG GTC CCT GGG GCG GCA TAT GCT TTT TAT GGC 2700 Tyr Ile Lys Gly Arg Leu Val Pro Gly Ala Ala Tyr Ala Phe Tyr Gly 780 785 790 GTG TGG CCG CTG CTC CTG CTC CTG TTG GCG TTA CCA CCA CGG GCA TAC 2748 Val Trp Pro Leu Leu Leu Leu Leu Leu Ala Leu Pro Pro Arg Ala Tyr 795 800 805 GCC ATG GAC CGG GAG ATG GCC GCA TCG TGC GGA GGC GCG GTT TTC GTA 2796 Ala Met Asp Arg Glu Met Ala Ala Ser Cys Gly Gly Ala Val Phe Val 810 815 820 GGT CTA GCA CTC TTG ACC TTG TCA CCA CAC TAT AAA GCA TTC CTC GCC 2844 Gly Leu Ala Leu Leu Thr Leu Ser Pro His Tyr Lys Ala Phe Leu Ala 825 830 835 840 AGG CTC ATA TGG TGG TTA CAA TAT TTT ATC ACC AGG GTC GAG GCG TGC 2892 Arg Leu Ile Trp Trp Leu Gln Tyr Phe Ile Thr Arg Val Glu Ala Cys 845 850 855 TTG CAA GTG TGG GTC CCC CCT CTC AAC GTC CGG GGG GGC CGC GAT GCC 2940 Leu Gln Val Trp Val Pro Pro Leu Asn Val Arg Gly Gly Arg Asp Ala 860 865 870 ATC ATC CTC CTC ACG TGC GCG GTC CAC TCA GAG CTA ATC TTT GAC ATC 2988 Ile Ile Leu Leu Thr Cys Ala Val His Ser Glu Leu Ile Phe Asp Ile 875 880 885 ACC AAA ATT CTG CTC GCC ATA TTT GGA CCG CTC ATG GTG CTC CAG GCT 3036 Thr Lys Ile Leu Leu Ala Ile Phe Gly Pro Leu Met Val Leu Gln Ala 890 895 900 GGC CTA ACT AGA GTG CCG TAC TTC GTG CGC GCT CAA GGG CTC CTT CGT 3084 Gly Leu Thr Arg Val Pro Tyr Phe Val Arg Ala Gln Gly Leu Leu Arg 905 910 915 920 GCA TGC ATG TTG GTG CGG AAG GTC GCT GGG GGC CAT TAT GTC CAA ATG 3132 Ala Cys Met Leu Val Arg Lys Val Ala Gly Gly His Tyr Val Gln Met 925 930 935 GCT CTC ATG AAG CTG GCC GCA CTG ACT GGG ACG TAC GTT TAT GAC CAT 3180 Ala Leu Met Lys Leu Ala Ala Leu Thr Gly Thr Tyr Val Tyr Asp His 940 945 950 CTT ACT CCG CTG CGG GAC TGG GCC CAC TCG GGC CTG CGA GAC CTT GTG 3228 Leu Thr Pro Leu Arg Asp Trp Ala His Ser Gly Leu Arg Asp Leu Val 955 960 965 GTA GCG GTC GAG CCC GTC GTC TTC TCC GAC ATG GAG ACC AAG ATC ATC 3276 Val Ala Val Glu Pro Val Val Phe Ser Asp Met Glu Thr Lys Ile Ile 970 975 980 ACC TGG GGG GCG GAC ACA GCG GCG TGT GGG GAC ATC ATC TTG GGT CTG 3324 Thr Trp Gly Ala Asp Thr Ala Ala Cys Gly Asp Ile Ile Leu Gly Leu 985 990 995 1000 CCC GTC TCC GCC CGA AGG GGG AGG GAG ATA CTT CTG GGA CCG GCC GAT 3372 Pro Val Ser Ala Arg Arg Gly Arg Glu Ile Leu Leu Gly Pro Ala Asp 1005 1010 1015 GGT TTC AGA GAG CAG GGA TGG CGA CTC CTT GCG CCC ATC ACG GCC TAC 3420 Gly Phe Arg Glu Gln Gly Trp Arg Leu Leu Ala Pro Ile Thr Ala Tyr 1020 1025 1030 TCC CAA CAG ACG CGG GGC CTA TTT GGC TGC ATC GTC ACT AGT CTC ACA 3468 Ser Gln Gln Thr Arg Gly Leu Phe Gly Cys Ile Val Thr Ser Leu Thr 1035 1040 1045 GGT CGG GAC AAG AAC CAG GTC GAA GGG GAG GCT CAG GTG GTT TCC ACC 3516 Gly Arg Asp Lys Asn Gln Val Glu Gly Glu Ala Gln Val Val Ser Thr 1050 1055 1060 GCA ACG CAA TCT TTC TTG GCG ACC TGT GTC AAC GGC GTG TGT TGG ACT 3564 Ala Thr Gln Ser Phe Leu Ala Thr Cys Val Asn Gly Val Cys Trp Thr 1065 1070 1075 1080 GTT TAC CAT GGC GCC GGC TCA AAG ACC CTA GCC GGC CCA AAG GGG CCA 3612 Val Tyr His Gly Ala Gly Ser Lys Thr Leu Ala Gly Pro Lys Gly Pro 1085 1090 1095 ATC AAC CAA ATG TAC ACT AAT GTG GAT CAG GAC CTC GTC GGC TGG CAG 3660 Ile Asn Gln Met Tyr Thr Asn Val Asp Gln Asp Leu Val Gly Trp Gln 1100 1105 1110 GCG CCC TCC GGG GCG GCT TCC TTG ACA CCG TGC ACC TAC GGC AGC TCG 3708 Ala Pro Ser Gly Ala Ala Ser Leu Thr Pro Cys Thr Tyr Gly Ser Ser 1115 1120 1125 GAC CTT TAC TTG GTC ACG AGG CAT GCT GAT GTC ATT CCG GTG CGC CGG 3756 Asp Leu Tyr Leu Val Thr Arg His Ala Asp Val Ile Pro Val Arg Arg 1130 1135 1140 CGG GGC GAC AGC AGG GGG AGT CTA CTT TCC CCC AGG CCC GTC TCC TAC 3804 Arg Gly Asp Ser Arg Gly Ser Leu Leu Ser Pro Arg Pro Val Ser Tyr 1145 1150 1155 1160 TTG AAG GGC TCT TCC GGT GGT CCA CTG CTC TGC CCC TCG GGG CAC GCT 3852 Leu Lys Gly Ser Ser Gly Gly Pro Leu Leu Cys Pro Ser Gly His Ala 1165 1170 1175 GTG GGC ATC TTC CGG GCT GCT GTG TGC ACC CGG GGG GTT GCG AAG GCG 3900 Val Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val Ala Lys Ala 1180 1185 1190 GTG GAC TTC ATA CCC GTT GAG TCT ATG GAA ACT ACC ATG CGG TCT CCG 3948 Val Asp Phe Ile Pro Val Glu Ser Met Glu Thr Thr Met Arg Ser Pro 1195 1200 1205 GTC TTC ACG GAT AAT TCT TCT CCC CCG GCC GTA CCG CAG ACA TTC CAA 3996 Val Phe Thr Asp Asn Ser Ser Pro Pro Ala Val Pro Gln Thr Phe Gln 1210 1215 1220 GTG GCC CAT CTA CAT GCT CCC ACT GGC AGC GGC AAG AGC ACT AAG GTG 4044 Val Ala His Leu His Ala Pro Thr Gly Ser Gly Lys Ser Thr Lys Val 1225 1230 1235 1240 CCG GCT GCA TAC GCA GCC CAA GGG TAC AAG GTA CTC GTC CTG AAC CCG 4092 Pro Ala Ala Tyr Ala Ala Gln Gly Tyr Lys Val Leu Val Leu Asn Pro 1245 1250 1255 TCC GTT GCC GCC ACC TTG GGT TTT GGG GCA TAC ATG TCC AAG GCA CAT 4140 Ser Val Ala Ala Thr Leu Gly Phe Gly Ala Tyr Met Ser Lys Ala His 1260 1265 1270 GGT GTT GAC CCT AAC ATC AGT ACT GGG GTA AGG ACC ATC ACC ACG GGC 4188 Gly Val Asp Pro Asn Ile Ser Thr Gly Val Arg Thr Ile Thr Thr Gly 1275 1280 1285 GCC CCT ATC ACG TAC TCC ACC TAC GGC AAG TTC CTT GCC GAC GGT GGT 4236 Ala Pro Ile Thr Tyr Ser Thr Tyr Gly Lys Phe Leu Ala Asp Gly Gly 1290 1295 1300 TGC TCC GGG GGC GCC TAT GAC ATC ATA ATA TGT GAC GAG TGC CAT TCA 4284 Cys Ser Gly Gly Ala Tyr Asp Ile Ile Ile Cys Asp Glu Cys His Ser 1305 1310 1315 1320 ACT GAC TCG ACT TCT ATC TTA GGC ATC GGC ACG GTC CTG GAC CAA GCG 4332 Thr Asp Ser Thr Ser Ile Leu Gly Ile Gly Thr Val Leu Asp Gln Ala 1325 1330 1335 GAG ACG GCT GGA GCG CGG CTC GTC GTG CTC GCC GCC GCT ACG CCT CCG 4380 Glu Thr Ala Gly Ala Arg Leu Val Val Leu Ala Ala Ala Thr Pro Pro 1340 1345 1350 GGA TCG GTC ACC GTG CCA CAT CCC AAC ATT GAG GAG GTG GCC TTG CCT 4428 Gly Ser Val Thr Val Pro His Pro Asn Ile Glu Glu Val Ala Leu Pro 1355 1360 1365 AAC ACC GGA GAG ATT CCC TTC TAT GGC AAA GCC ATC CCC CTC GAG ACC 4476 Asn Thr Gly Glu Ile Pro Phe Tyr Gly Lys Ala Ile Pro Leu Glu Thr 1370 1375 1380 ATC AAG GGG GGG AGG CAT CTC ATC TTC TGC CAT TCC AAG AAG AAG TGT 4524 Ile Lys Gly Gly Arg His Leu Ile Phe Cys His Ser Lys Lys Lys Cys 1385 1390 1395 1400 GAT GAG CTC GCT GCA AAG CTG TCG GCC CTC GGG GTC AAT GCT GTA GCG 4572 Asp Glu Leu Ala Ala Lys Leu Ser Ala Leu Gly Val Asn Ala Val Ala 1405 1410 1415 TAC TAC CGG GGC CTT GAT GTG TCC GTC ATA CCG ACA AGC GGA GAC GTC 4620 Tyr Tyr Arg Gly Leu Asp Val Ser Val Ile Pro Thr Ser Gly Asp Val 1420 1425 1430 GTT GTC GTG GCA ACA GAC GCC CTA ATG ACA GGC TAC ACC GGT GAC TTT 4668 Val Val Val Ala Thr Asp Ala Leu Met Thr Gly Tyr Thr Gly Asp Phe 1435 1440 1445 GAT TCA GTG ATC GAC TGT AAT ACA TGT GTC ACC CAG ACA GTC GAC TTC 4716 Asp Ser Val Ile Asp Cys Asn Thr Cys Val Thr Gln Thr Val Asp Phe 1450 1455 1460 AGC TTG GAC CCT ACC TTC ACC ATT GAG ACG ACA ACG CTG CCC CAA GAC 4764 Ser Leu Asp Pro Thr Phe Thr Ile Glu Thr Thr Thr Leu Pro Gln Asp 1465 1470 1475 1480 GCG GTG TCG CGC TCA CAA CGG CGA GGC AGA ACT GGC AGG GGT AGA GGG 4812 Ala Val Ser Arg Ser Gln Arg Arg Gly Arg Thr Gly Arg Gly Arg Gly 1485 1490 1495 GGC ATA TAC AGG TTT GTG ACT CCA GGG GAA CGG CCT TCG GGT ATG TTC 4860 Gly Ile Tyr Arg Phe Val Thr Pro Gly Glu Arg Pro Ser Gly Met Phe 1500 1505 1510 GAT TCT TCG GTC CTA TGT GAG TGC TAT GAC GCG GGC TGT GCT TGG TAC 4908 Asp Ser Ser Val Leu Cys Glu Cys Tyr Asp Ala Gly Cys Ala Trp Tyr 1515 1520 1525 GAG CTC ACG CCC GCA GTG ACC TCG GTT AGG CTG CGG GCT TAC CTA AAT 4956 Glu Leu Thr Pro Ala Val Thr Ser Val Arg Leu Arg Ala Tyr Leu Asn 1530 1535 1540 ACA CCA GGG TTG CCC GTC TGC CAG GTC CAC CTG GAG TTC TGG GAG AGC 5004 Thr Pro Gly Leu Pro Val Cys Gln Val His Leu Glu Phe Trp Glu Ser 1545 1550 1555 1560 GTC TTT ACA GGC CTC ACC CAC ATA GAT GCC CAC TTC TTG TCC CAG ACT 5052 Val Phe Thr Gly Leu Thr His Ile Asp Ala His Phe Leu Ser Gln Thr 1565 1570 1575 AAG CAG GCA GGA GAG AAC TTC CCC TAC CTG GTG GCA TAC CAG GCC ACA 5100 Lys Gln Ala Gly Glu Asn Phe Pro Tyr Leu Val Ala Tyr Gln Ala Thr 1580 1585 1590 GTG TGC GCC AGG GCC CAG GCC CCA CCT CCA TCG TGG GAT CAA ATG TGG 5148 Val Cys Ala Arg Ala Gln Ala Pro Pro Pro Ser Trp Asp Gln Met Trp 1595 1600 1605 AAG TGT CTC ATA CGG CTA AAG CCT ACG CTG CAC GGG CCA ACA CCC CTG 5196 Lys Cys Leu Ile Arg Leu Lys Pro Thr Leu His Gly Pro Thr Pro Leu 1610 1615 1620 TTG TAT AGG CTA GGA GCC GTC CAA AAC GAG GTC ACC CTC ACA CAC CCC 5244 Leu Tyr Arg Leu Gly Ala Val Gln Asn Glu Val Thr Leu Thr His Pro 1625 1630 1635 1640 ATA ACC AAA TTC ATC ATG GCA TGC ATG TCG GCT GAT CTA GAG GTC GTC 5292 Ile Thr Lys Phe Ile Met Ala Cys Met Ser Ala Asp Leu Glu Val Val 1645 1650 1655 ACC AGC ACC TGG GTG CTG GTA GGC GGA GTC CTC GCA GCT CTG GCC GCG 5340 Thr Ser Thr Trp Val Leu Val Gly Gly Val Leu Ala Ala Leu Ala Ala 1660 1665 1670 TAC TGC CTG ACA ACG GGC AGC GTG GTT ATT GTG GGC AGG ATC ATC TTG 5388 Tyr Cys Leu Thr Thr Gly Ser Val Val Ile Val Gly Arg Ile Ile Leu 1675 1680 1685 TCC GGG AGG CCG GCT ATC ATT CCT GAC AGG GAA GTC CTC TAC CAG GAG 5436 Ser Gly Arg Pro Ala Ile Ile Pro Asp Arg Glu Val Leu Tyr Gln Glu 1690 1695 1700 TTT GAT GAG ATG GAG GAG TGC GCC TCA CAC CTT CCT TAC ATC GAA CAA 5484 Phe Asp Glu Met Glu Glu Cys Ala Ser His Leu Pro Tyr Ile Glu Gln 1705 1710 1715 1720 GGA ATG CAG CTC GCC GAG CAA TTC AAG CAG AAG GCG CTC GGG CTG CTG 5532 Gly Met Gln Leu Ala Glu Gln Phe Lys Gln Lys Ala Leu Gly Leu Leu 1725 1730 1735 CAA ACA GCC TCC AAG CAA GCG GAG GCT GCT GCT CCT GTG GTA GAG TCC 5580 Gln Thr Ala Ser Lys Gln Ala Glu Ala Ala Ala Pro Val Val Glu Ser 1740 1745 1750 AAG TGG CAA GCC CTT GAG GCC TTC TGG GCG AAG CAC ATG TGG AAC TTC 5628 Lys Trp Gln Ala Leu Glu Ala Phe Trp Ala Lys His Met Trp Asn Phe 1755 1760 1765 ATC AGC GGG ATA CAG TAT CTA GCA GGC TTG TCC ACC CTG CCT GGA AAC 5676 Ile Ser Gly Ile Gln Tyr Leu Ala Gly Leu Ser Thr Leu Pro Gly Asn 1770 1775 1780 CCC GCA ATA GTA TCA CTG ATG GCA TTC ACA GCC TCT ATC ACT AGC CCG 5724 Pro Ala Ile Val Ser Leu Met Ala Phe Thr Ala Ser Ile Thr Ser Pro 1785 1790 1795 1800 CTC ACC ACC CAA CAT ACC CTC CTG TTT AAC ATC TTA GGG GGA TGG GTG 5772 Leu Thr Thr Gln His Thr Leu Leu Phe Asn Ile Leu Gly Gly Trp Val 1805 1810 1815 GCC GCC CAA CTC GCC CCC CCC AGC GCT GCT TCA GCC TTC GTG GGC GCC 5820 Ala Ala Gln Leu Ala Pro Pro Ser Ala Ala Ser Ala Phe Val Gly Ala 1820 1825 1830 GGC ATC GCT GGC GCG GCT GTT GGC AGC ATA GGC CTT GGG AAG GTG CTT 5868 Gly Ile Ala Gly Ala Ala Val Gly Ser Ile Gly Leu Gly Lys Val Leu 1835 1840 1845 GTG GAC ATC CTG GCG GGT TAT GGA GCA GGG GTG GCA GGC GCA CTC GTG 5916 Val Asp Ile Leu Ala Gly Tyr Gly Ala Gly Val Ala Gly Ala Leu Val 1850 1855 1860 GCC TTT AAG GGC ATG AGC GGC GAA ATG CCC TCC ACC GAG GAC CTG GTC 5964 Ala Phe Lys Gly Met Ser Gly Glu Met Pro Ser Thr Glu Asp Leu Val 1865 1870 1875 1880 AAC TTA CTC CCT GCC ATC CTC TCT CCT GGC GCC CTG GTC GTC GGG GTC 6012 Asn Leu Leu Pro Ala Ile Leu Ser Pro Gly Ala Leu Val Val Gly Val 1885 1890 1895 GTG TGC GCA GCA ATA CTG CGT CGG CAT GTG GGC CCA GGG GAG GGG GCT 6060 Val Cys Ala Ala Ile Leu Arg Arg His Val Gly Pro Gly Glu Gly Ala 1900 1905 1910 GTG CAG TGG ATG AAC CGG CTG ATA GCA TTC GCC TCG CGG GGT AAC CAC 6108 Val Gln Trp Met Asn Arg Leu Ile Ala Phe Ala Ser Arg Gly Asn His 1915 1920 1925 GTT TCC CCC ACG CAT TAT GTG CCT GAG AGC GAT GCT GCG GCG CGT GTC 6156 Val Ser Pro Thr His Tyr Val Pro Glu Ser Asp Ala Ala Ala Arg Val 1930 1935 1940 ACC AAG ATC CTC TCC AGC CTT ACC ATC ACT CAG CGT CTA AGG AGG CTC 6204 Thr Lys Ile Leu Ser Ser Leu Thr Ile Thr Gln Arg Leu Arg Arg Leu 1945 1950 1955 1960 CAC CAG TGG ATT AAT GAG GAC TGC TCC ACG CCA TGC TCC GGT TCG TGG 6252 His Gln Trp Ile Asn Glu Asp Cys Ser Thr Pro Cys Ser Gly Ser Trp 1965 1970 1975 CTC AGG GAT GTT TGG GAC TGG ATA TGC ACG GTG TTG ACT GAC TTC AAG 6300 Leu Arg Asp Val Trp Asp Trp Ile Cys Thr Val Leu Thr Asp Phe Lys 1980 1985 1990 ACC TGG CTC CAG TCC AAG CTC CTG CCG CGG CTG CCG GGA GAC CCC TTC 6348 Thr Trp Leu Gln Ser Lys Leu Leu Pro Arg Leu Pro Gly Asp Pro Phe 1995 2000 2005 TTC TCA TGT CAA CGT GGA TAC AGG GGG GTC TGG CGG GGA GAT GGC GTC 6396 Phe Ser Cys Gln Arg Gly Tyr Arg Gly Val Trp Arg Gly Asp Gly Val 2010 2015 2020 ATG CAA ACC ACC TGC CCA TGT GGA GCA CAG ATC ACC GGA CAT GTC AAA 6444 Met Gln Thr Thr Cys Pro Cys Gly Ala Gln Ile Thr Gly His Val Lys 2025 2030 2035 2040 AAC GGT TCC ATG AGG ATC GTT GGG CCT AAA ACC TGT AGC AAC ACG TGG 6492 Asn Gly Ser Met Arg Ile Val Gly Pro Lys Thr Cys Ser Asn Thr Trp 2045 2050 2055 CAC GGA ACA TTC CCC ATC AAC GCA TAC ACC ACG GGC CCC TGC ACG CCC 6540 His Gly Thr Phe Pro Ile Asn Ala Tyr Thr Thr Gly Pro Cys Thr Pro 2060 2065 2070 TCC CCG GCG CCA AAC TAT TCC AGG GCG CTG TGG CGG GTG GCT GCT GAG 6588 Ser Pro Ala Pro Asn Tyr Ser Arg Ala Leu Trp Arg Val Ala Ala Glu 2075 2080 2085 GAG TAT GTG GAA GTC ACG CGG GTG GGG GAT TTC CAC TAC GTG ACG GGC 6636 Glu Tyr Val Glu Val Thr Arg Val Gly Asp Phe His Tyr Val Thr Gly 2090 2095 2100 ATG ACC ACT GAC AAC GTA AAA TGC CCA TGC CAG GTT CCA GCC CCC GAA 6684 Met Thr Thr Asp Asn Val Lys Cys Pro Cys Gln Val Pro Ala Pro Glu 2105 2110 2115 2120 TTC TTC ACG GAA GTG GAT GGG GTG CGG TTG CAC AGG TAC GCT CCA GCT 6732 Phe Phe Thr Glu Val Asp Gly Val Arg Leu His Arg Tyr Ala Pro Ala 2125 2130 2135 TGC AAA CCT CTC CTA CGG GAT GAG GTT ACA TTC CAG GTC GGG CTC AAC 6780 Cys Lys Pro Leu Leu Arg Asp Glu Val Thr Phe Gln Val Gly Leu Asn 2140 2145 2150 CAA TTC CCG GTT GGG TCA CAG CTC CCG TGT GAG CCC GAA CCG GAT GTA 6828 Gln Phe Pro Val Gly Ser Gln Leu Pro Cys Glu Pro Glu Pro Asp Val 2155 2160 2165 ACA GTG CTC ACT TCC ATG CTT ACC GAC CCC TCC CAC ATT ACA GCG GAG 6876 Thr Val Leu Thr Ser Met Leu Thr Asp Pro Ser His Ile Thr Ala Glu 2170 2175 2180 ACG GCT AAG CGT AGG TTG GCC AGA GGG TCT CCC CCC TCC TTG GCC AGC 6924 Thr Ala Lys Arg Arg Leu Ala Arg Gly Ser Pro Pro Ser Leu Ala Ser 2185 2190 2195 2200 TCT TCA GCT AGC CAG TTG TCT GCG CCT TCT TTG AAG GCG ACA TGC ACT 6972 Ser Ser Ala Ser Gln Leu Ser Ala Pro Ser Leu Lys Ala Thr Cys Thr 2205 2210 2215 ACC CGT CAT GAC TCC CCG GAC GCC GAC CTC ATC GAG GCC AAC CTC CTG 7020 Thr Arg His Asp Ser Pro Asp Ala Asp Leu Ile Glu Ala Asn Leu Leu 2220 2225 2230 TGG CGG CAG GAG ATG GGC GGG AAC ATC ACC CGG GTG GAG TCA GAG AAT 7068 Trp Arg Gln Glu Met Gly Gly Asn Ile Thr Arg Val Glu Ser Glu Asn 2235 2240 2245 AAG GTA GTA ATT CTA GAC TCT TTT GAA CCG CTT CGG GCG GAG GAG GAT 7116 Lys Val Val Ile Leu Asp Ser Phe Glu Pro Leu Arg Ala Glu Glu Asp 2250 2255 2260 GAG AGG GAA GTA TCC GTG GCG GCG GAG ATC CTG CGG AAA AGC AGG AAG 7164 Glu Arg Glu Val Ser Val Ala Ala Glu Ile Leu Arg Lys Ser Arg Lys 2265 2270 2275 2280 TTC CCC CCA GCG TTG CCC ATA TGG GCA CGC CCG AGC TAC AAC CCA CCA 7212 Phe Pro Pro Ala Leu Pro Ile Trp Ala Arg Pro Ser Tyr Asn Pro Pro 2285 2290 2295 CTC CTA GAG TCT TGG AAG GAC CCG GAC TAC GTC CCT CCG GTG GTA CAC 7260 Leu Leu Glu Ser Trp Lys Asp Pro Asp Tyr Val Pro Pro Val Val His 2300 2305 2310 GGG TGC CCT CTG CCA CCT ACC ATG GCC CCT CCA ATA CCA CCT CCA CGG 7308 Gly Cys Pro Leu Pro Pro Thr Met Ala Pro Pro Ile Pro Pro Pro Arg 2315 2320 2325 AGG AAG AGG ACG GTT GTC CTG ACA GAA TCC ACC GTG TCT TCT GCC TTG 7356 Arg Lys Arg Thr Val Val Leu Thr Glu Ser Thr Val Ser Ser Ala Leu 2330 2335 2340 GCT GAG CTT GCC ACA AAG ACC TTC GGC AGC TCC GGA TCG TCA GCC GTT 7404 Ala Glu Leu Ala Thr Lys Thr Phe Gly Ser Ser Gly Ser Ser Ala Val 2345 2350 2355 2360 GAC AGC GGC ACG GCG ACG GCC CCT CCT GAC CAG CCC TCC GAT GAC GGA 7452 Asp Ser Gly Thr Ala Thr Ala Pro Pro Asp Gln Pro Ser Asp Asp Gly 2365 2370 2375 GAC AGA GGA TCT GAC GAT GAG TCG TAC TCC TCC ATG CCC CCC CTT GAG 7500 Asp Arg Gly Ser Asp Asp Glu Ser Tyr Ser Ser Met Pro Pro Leu Glu 2380 2385 2390 GGG GAG CCG GGG GAC CCC GAT CTC AGC GAC GGG TCT TGG TCT ACC GTG 7548 Gly Glu Pro Gly Asp Pro Asp Leu Ser Asp Gly Ser Trp Ser Thr Val 2395 2400 2405 AGC GAG GAG GCT AGT NNN GAG GAC GTC GCT TGC TGC TCG ATG TCT TAC 7596 Ser Glu Glu Ala Ser Xaa Glu Asp Val Ala Cys Cys Ser Met Ser Tyr 2410 2415 2420 ACG TGG ACA GGC GCC CTA ATC ACA CCA TGC GCC GCG GAG GAG AGC AAG 7644 Thr Trp Thr Gly Ala Leu Ile Thr Pro Cys Ala Ala Glu Glu Ser Lys 2425 2430 2435 2440 CTG CCC ATC AAT CCG TTG AGC AAC TCT TTG CTG CGT CAC CAC AAC ATG 7692 Leu Pro Ile Asn Pro Leu Ser Asn Ser Leu Leu Arg His His Asn Met 2445 2450 2455 GTC TAC GCC ACA ACA TCT CGC AGC GCA GGC CTA CGG CAG AAA AAG GTC 7740 Val Tyr Ala Thr Thr Ser Arg Ser Ala Gly Leu Arg Gln Lys Lys Val 2460 2465 2470 ACC TTT GAC AGA CTG CAG GTC CCG GAC GAC CAT TAC CGG GAC GTG CTC 7788 Thr Phe Asp Arg Leu Gln Val Pro Asp Asp His Tyr Arg Asp Val Leu 2475 2480 2485 AAG GAG ATG AAG GCG AAG GCG TCC ACA GTT AAG GCT AAA CTT CTA TCT 7836 Lys Glu Met Lys Ala Lys Ala Ser Thr Val Lys Ala Lys Leu Leu Ser 2490 2495 2500 GTA GAA GAA GCC TGC AAG CTG ACG CCC CCA CAC TCG GCC AGA TCC AAA 7884 Val Glu Glu Ala Cys Lys Leu Thr Pro Pro His Ser Ala Arg Ser Lys 2505 2510 2515 2520 TTT GGC TAT GGG GCG AAG GAC GTC CGG AAC CTA TCC AGC AAG GCC GTT 7932 Phe Gly Tyr Gly Ala Lys Asp Val Arg Asn Leu Ser Ser Lys Ala Val 2525 2530 2535 AAC CAC ATC CAC TCC GTG TGG AAG GAC TTG CTG GAA GAC ACT GAA ACA 7980 Asn His Ile His Ser Val Trp Lys Asp Leu Leu Glu Asp Thr Glu Thr 2540 2545 2550 CCA ATT GAC ACT ACC ATC ATG GCA AAA AAT GAG GTC TTC TGT GTT CAA 8028 Pro Ile Asp Thr Thr Ile Met Ala Lys Asn Glu Val Phe Cys Val Gln 2555 2560 2565 CCA GAG AAA GGA GGC CGC AAG CCA GCC CGC CTT ATC GTA TTC CCA GAA 8076 Pro Glu Lys Gly Gly Arg Lys Pro Ala Arg Leu Ile Val Phe Pro Glu 2570 2575 2580 CTT GGG GTT CGT GTG TGC GAG AAA ATG GCC CTT TAC GAC GTG GTC TCC 8124 Leu Gly Val Arg Val Cys Glu Lys Met Ala Leu Tyr Asp Val Val Ser 2585 2590 2595 2600 ACT CTT CCT CAG GCC GTG ATG GGC TCC TCA TAC GGA TTC CAG TAC TCT 8172 Thr Leu Pro Gln Ala Val Met Gly Ser Ser Tyr Gly Phe Gln Tyr Ser 2605 2610 2615 CCT GGG CAG CGG GTC GAG TTC CTG GTG AAT GCC TGG AAA TCG AAG AAA 8220 Pro Gly Gln Arg Val Glu Phe Leu Val Asn Ala Trp Lys Ser Lys Lys 2620 2625 2630 AAC CCT ATG GGC TTC GCA TAT TGC ACC CGC TGT TTT GAC TCA ACG GTC 8268 Asn Pro Met Gly Phe Ala Tyr Cys Thr Arg Cys Phe Asp Ser Thr Val 2635 2640 2645 ACT GAG AGT GAT ATC CGT GTT GAG GAG TCA ATT TAC CAA TGT TGT GAC 8316 Thr Glu Ser Asp Ile Arg Val Glu Glu Ser Ile Tyr Gln Cys Cys Asp 2650 2655 2660 TTG GCC CCC GAG GCC AGA CAG GTC ATA AGG TCG CTC ACG GAG CGG CTT 8364 Leu Ala Pro Glu Ala Arg Gln Val Ile Arg Ser Leu Thr Glu Arg Leu 2665 2670 2675 2680 TAT ATC GGG GGC CCC CTG ACT AAT TCA AAA GGG CAG AAC TGC GGT TAT 8412 Tyr Ile Gly Gly Pro Leu Thr Asn Ser Lys Gly Gln Asn Cys Gly Tyr 2685 2690 2695 CGC CGG TGC CGC GCC AGC GGT GTG CTG ACG ACT AAC TGC GGT AAT ACC 8460 Arg Arg Cys Arg Ala Ser Gly Val Leu Thr Thr Asn Cys Gly Asn Thr 2700 2705 2710 CTC ACA TGT TAC TTG AAG GCC TCT GCA GCC TGT CGA GCT GCA AAG CTC 8508 Leu Thr Cys Tyr Leu Lys Ala Ser Ala Ala Cys Arg Ala Ala Lys Leu 2715 2720 2725 CAG GAC TGC ACG ATG CTC GTG TGC GGA GAC GAC CTT GTC GTT ATC TGT 8556 Gln Asp Cys Thr Met Leu Val Cys Gly Asp Asp Leu Val Val Ile Cys 2730 2735 2740 GAA AGC GCG GGA ACC CAG GAG GAC GCG GCG AGC CTA CGA GTC TTC ACG 8604 Glu Ser Ala Gly Thr Gln Glu Asp Ala Ala Ser Leu Arg Val Phe Thr 2745 2750 2755 2760 GAG GCT ATG ACT AGG TAC TCT GCC CCC CCC GGG GAC CCG CCC CAA CCA 8652 Glu Ala Met Thr Arg Tyr Ser Ala Pro Pro Gly Asp Pro Pro Gln Pro 2765 2770 2775 GAA TAC GAC TTG GAG TTA ATA ACA TCA TGC TCC TCC AAC GTG TCG GTC 8700 Glu Tyr Asp Leu Glu Leu Ile Thr Ser Cys Ser Ser Asn Val Ser Val 2780 2785 2790 GCG CAC GAC GCA TCT GGC AAG CGG GTG TAC TAC CTC ACT CGC GAC CCC 8748 Ala His Asp Ala Ser Gly Lys Arg Val Tyr Tyr Leu Thr Arg Asp Pro 2795 2800 2805 ACC ACC CCC CTC GCG AGG GCA GCG TGG GAA ACA GCA AGA CAC ACT CCA 8796 Thr Thr Pro Leu Ala Arg Ala Ala Trp Glu Thr Ala Arg His Thr Pro 2810 2815 2820 GTA AAC TCC TGG CTA GGC AAC ATC ATC ATG TAC GCG CCC ACC CTG TGG 8844 Val Asn Ser Trp Leu Gly Asn Ile Ile Met Tyr Ala Pro Thr Leu Trp 2825 2830 2835 2840 GCA AGG ATG ATT CTG ATG ACC CAC TTC TTC TCC ATC CTT CTA GCT CAG 8892 Ala Arg Met Ile Leu Met Thr His Phe Phe Ser Ile Leu Leu Ala Gln 2845 2850 2855 GAG CAA CTT GAA AAA GCC CTG GGT TGT CAG ATC TAC GGG GCC ACT TAC 8940 Glu Gln Leu Glu Lys Ala Leu Gly Cys Gln Ile Tyr Gly Ala Thr Tyr 2860 2865 2870 TTC ATT GAA CCA CTT GAC CTA CCT CAG ATC ATT CAG CGA CTC CAC GGT 8988 Phe Ile Glu Pro Leu Asp Leu Pro Gln Ile Ile Gln Arg Leu His Gly 2875 2880 2885 CTT AGC GCA TTT TCA CTC CAC AGT TAC TCT CCA GGT GAA ATC AAT AGG 9036 Leu Ser Ala Phe Ser Leu His Ser Tyr Ser Pro Gly Glu Ile Asn Arg 2890 2895 2900 GTG GCT TCA TGC CTC AGG AAA CTT GGG GTA CCA CCC TTG CGA GTC TGG 9084 Val Ala Ser Cys Leu Arg Lys Leu Gly Val Pro Pro Leu Arg Val Trp 2905 2910 2915 2920 AGA CAT CGG GCC AGA AGT GTC CGC GCT AAG CTA CTG TCC CAG GGG GGG 9132 Arg His Arg Ala Arg Ser Val Arg Ala Lys Leu Leu Ser Gln Gly Gly 2925 2930 2935 AGG GCC GCC ACT TGT GGC AAG TAC CTC TTC AAC TGG GCA GTG AGG ACC 9180 Arg Ala Ala Thr Cys Gly Lys Tyr Leu Phe Asn Trp Ala Val Arg Thr 2940 2945 2950 AAG CTT AAA CTC ACT CCA ATT CCG GCT GCG TCC CAG TTG GAC TTA TCC 9228 Lys Leu Lys Leu Thr Pro Ile Pro Ala Ala Ser Gln Leu Asp Leu Ser 2955 2960 2965 GGC TGG TTC GTT GCT GGT TAC AGC GGG GGA GAC ATA TAT CAC AGC CTG 9276 Gly Trp Phe Val Ala Gly Tyr Ser Gly Gly Asp Ile Tyr His Ser Leu 2970 2975 2980 TCT CGT GCC CGA CCC CGC TGG TTC ATG TGG TGC CTA CTC CTA CTT TCT 9324 Ser Arg Ala Arg Pro Arg Trp Phe Met Trp Cys Leu Leu Leu Leu Ser 2985 2990 2995 3000 GTA GGG GTA GGC ATC TAC CTG CTC CCC AAC CGG TGA ACGGGGAGCT 9370 Val Gly Val Gly Ile Tyr Leu Leu Pro Asn Arg *** 3005 3010 AACCACTCCA GGCCAATAGG CCATTCCCTT TTTTT 9405 配列番号：３ 配列の長さ：１７３４ 配列の型：核酸 鎖の数：二本鎖 トポロジー：直鎖状 配列の種類：cDNA to genomic RNA ハイポセティカル：Ｎｏ アンチセンス：Ｎｏ 起源 生物名：Hepatitis C Virus 単離クローン名：JK1-C 組織の種類：肝臓細胞 GGCGACACTC CACCATAGAT CACTCCCCTG TGAGGAACTG CTGTCTTCAC GACGAAAGCG 60 TCTAGCCATG GCGTTAGTAT GACTGTCGTG CAGCCTGCAG GACCCCCCCT CCCGGGAGAG 120 CCATAGTGGT CTGCGGAACC GGTGAGTACA CCGGAATTGC CAGGACGACC GGGTCCTTTC 180 TTGGATCACC CCGCTCAATG CCTGGAGATT TGGGCGTGCC CCCGCGAGAC TGCTAGCCGA 240 GTAGTGTTGG GTCGCGAAAG GCCTTGTGGT ACTGCCTGAT AGGGTGCTTG CGAGTGCCCC 300 GGGAGGTCTC GTAGACCGTG CACC ATG AGC ACA AAT CCT AAA CCC CAA 348 Met Ser Thr Asn Pro Lys Pro Gln 1 5 AGA AAA ACC AAA CGT AAC ACC AAC CGC CGC CCA CAG GAC GTC AAG TTC 396 Arg Lys Thr Lys Arg Asn Thr Asn Arg Arg Pro Gln Asp Val Lys Phe 10 15 20 CCG GGC GGT GGT CAG ATC GTT GGT GGG GTT TAC CTG TTG CCG CGC AGG 444 Pro Gly Gly Gly Gln Ile Val Gly Gly Val Tyr Leu Leu Pro Arg Arg 25 30 35 40 GGC CCC AGG TTG GGT GTG CGC GCG ACT AGG AAG ACT TCC GAG CGG TCG 492 Gly Pro Arg Leu Gly Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Ser 45 50 55 CAA CCT CGT GGA AGG CGA CAA CCT ATC CCT AAG GCT CGC CAG CCC GAG 540 Gln Pro Arg Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Gln Pro Glu 60 65 70 GGC AGG GCC TGG GCT CAG CCC GGG TAC CCT TGG CCC CTC TAT GGC AAT 588 Gly Arg Ala Trp Ala Gln Pro Gly Tyr Pro Trp Pro Leu Tyr Gly Asn 75 80 85 GAG GGT CTG GGG TGG GCA GGA TGG CTC CTG TCA CCC TAC GGC TCC CGG 636 Glu Gly Leu Gly Trp Ala Gly Trp Leu Leu Ser Pro Tyr Gly Ser Arg 90 95 100 CCT AGG TGG GGC CCC ACG GAC CCC CGG CGT AGG TCG CGT AAT TTG GGT 684 Pro Arg Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Asn Leu Gly 105 110 115 120 AAG GTC ATC GAT ACC CTC ACA TGC GGC TTC GCC GAC CTC ATG GGG TAC 732 Lys Val Ile Asp Thr Leu Thr Cys Gly Phe Ala Asp Leu Met Gly Tyr 125 130 135 ATT CCG CTC GTC GGG GCC CCC CTA GGA GGC GCT GCC AGG GCC CTG GCG 780 Ile Pro Leu Val Gly Ala Pro Leu Gly Gly Ala Ala Arg Ala Leu Ala 140 145 150 CAT GGC GTC CGG GTT CTG GAG GAC GGC GTG AAC TAC GCA ACA GGG AAT 828 His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 155 160 165 CTG CCC GGT TGC TCT TTT TCT ATC TTC CTC TTG GCC TTG CTG TCC TGT 876 Leu Pro Gly Cys Ser Phe Ser Ile Phe Leu Leu Ala Leu Leu Ser Cys 170 175 180 CTG ACC GTC CCA GTT TCC ACT TAT GAA GTG CGC AAC GTG TCC GGG GTG 924 Leu Thr Val Pro Val Ser Thr Tyr Glu Val Arg Asn Val Ser Gly Val 185 190 195 200 TAC CAT GTC ACG AAC GAC TGC TCC AAC TCA AGT ATT GTG TAT GAG GCA 972 Tyr His Val Thr Asn Asp Cys Ser Asn Ser Ser Ile Val Tyr Glu Ala 205 210 215 GCG GAC ATG ATT ATG CAT ACC CCC GGG TGC GTA CCC TGC GTC CGG GAG 1020 Ala Asp Met Ile Met His Thr Pro Gly Cys Val Pro Cys Val Arg Glu 220 225 230 GGC AAT TCC TCC CGT TGC TGG GTA GCG CTC ACT CCC ACG CTC GCG GCC 1068 Gly Asn Ser Ser Arg Cys Trp Val Ala Leu Thr Pro Thr Leu Ala Ala 235 240 245 AGG AAC AGC AGC ATC CCC ACT ACG ACA ATA CGA CGA CAT GTC GAT TTG 1116 Arg Asn Ser Ser Ile Pro Thr Thr Thr Ile Arg Arg His Val Asp Leu 250 255 260 CTC GTT GGG GCA GCT GCT CTC TGC TCC GCT ATG TAC GTG GGG GAT CTC 1164 Leu Val Gly Ala Ala Ala Leu Cys Ser Ala Met Tyr Val Gly Asp Leu 265 270 275 280 TGT GGG TCT GTT TTC CTC GTC TCC CAA TTG TTC ACC TTC TCA CCT CGC 1212 Cys Gly Ser Val Phe Leu Val Ser Gln Leu Phe Thr Phe Ser Pro Arg 285 290 295 CAG TAT GAG ACG GTA CAG GAC TGC AAT TGC TCA CTC TAT CCC GGC CAC 1260 Gln Tyr Glu Thr Val Gln Asp Cys Asn Cys Ser Leu Tyr Pro Gly His 300 305 310 GTA TCA GGT CAC CGT ATG GCT TGG GAT ATG ATG ATG AAC TGG TCA CCT 1308 Val Ser Gly His Arg Met Ala Trp Asp Met Met Met Asn Trp Ser Pro 315 320 325 ACA ACG GCC TTA GTG GTG TCG CAG CTA CTC CGG ATC CCA CAA GCC GTC 1356 Thr Thr Ala Leu Val Val Ser Gln Leu Leu Arg Ile Pro Gln Ala Val 330 335 340 GTG GAC ATG GTG GTG GGG GCC CAC TGG GGA GTC CTG GCG GGC CTC GCC 1404 Val Asp Met Val Val Gly Ala His Trp Gly Val Leu Ala Gly Leu Ala 345 350 355 360 TAC TAT TCC ATG GTG GGA AAC TGG GCT AAG GTC TTG ATT GTG ATG CTA 1452 Tyr Tyr Ser Met Val Gly Asn Trp Ala Lys Val Leu Ile Val Met Leu 365 370 375 CTC TTT GCC GGC GTT GAC GGG ACT ACT ACC GTG TCA GTG GGG CAT GCA 1500 Leu Phe Ala Gly Val Asp Gly Thr Thr Thr Val Ser Val Gly His Ala 380 385 390 AGC CAG ACC ACC CGG CGC GTT GCG TCC TTC TTC AGT CCC GGC TCG GCT 1548 Ser Gln Thr Thr Arg Arg Val Ala Ser Phe Phe Ser Pro Gly Ser Ala 395 400 405 CAG AAA ATC CAA CTT GTT AAC ACC AAC GGC AGT TGG CAC ATC AAC AGG 1596 Gln Lys Ile Gln Leu Val Asn Thr Asn Gly Ser Trp His Ile Asn Arg 410 415 420 ACT GCC CTG AAT TGC AAC GAG TCC ATC AAC ACT GGG TTC TTT GCC GCG 1644 Thr Ala Leu Asn Cys Asn Glu Ser Ile Asn Thr Gly Phe Phe Ala Ala 425 430 435 440 CTG TTC TAC TCA AAG AAG TTC AAC TCG TCT GGA TGC TCA GAG CGC ATG 1692 Leu Phe Tyr Ser Lys Lys Phe Asn Ser Ser Gly Cys Ser Glu Arg Met 445 450 455 GCT AGC TGT CGT CCC ATT GAC AAA TTC GCT CAA GGG TGG GGT 1734 Ala Ser Cys Arg Pro Ile Asp Lys Phe Ala Gln Gly Trp Gly 460 465 470 配列番号：４ 配列の長さ：１７３４ 配列の型：核酸 鎖の数：二本鎖 トポロジー：直鎖状 配列の種類：cDNA to genomic RNA ハイポセティカル：Ｎｏ アンチセンス：Ｎｏ 起源 生物名：Hepatitis C Virus 単離クローン名：JK2-A 組織の種類：肝臓細胞 GGCGACACTC CACCATAGAT CACTCCCCTG TGAGGAACTG CTGTCTTCAC GACGAAAGCG 60 TCTAGCCATG GCGTTAGTAT GACTGTCGTG CAGCCTGCAG GACCCCCCCT CCCGGGAGAG 120 CCATAGTGGT CTGCGGAACC GGTGAGTACA CCGGAATTGC CAGGACGACC GGGTCCTTTC 180 TTGGATCACC CCGCTCAATG CCTGGAGATT TGGGCATGCC CCCGCGAGAC TGCTAGCCGA 240 GTAGTGTTGG GTCGCGAAAG GCCTTGTGGT ACTGCCTGAT AGGGTGCTTG CGAGTGCCCC 300 GGGAGGTCTC GTAGACCGTG CATC ATG AGC ACA AAT CCT AAA CCT CAA 348 Met Ser Thr Asn Pro Lys Pro Gln 1 5 AGA AAA ACC AAA CGT AAC ACC AAC CGC CGC CCA CAG GAC GTC AAG TTC 396 Arg Lys Thr Lys Arg Asn Thr Asn Arg Arg Pro Gln Asp Val Lys Phe 10 15 20 CCG GGC GGT GGC CAG ATC GTT GGT GGA GTT TAC CTG TTG CCG CGC AGG 444 Pro Gly Gly Gly Gln Ile Val Gly Gly Val Tyr Leu Leu Pro Arg Arg 25 30 35 40 GGC CCC AGG TTG GGT GTG CGC GCG ACT AGG AAG ACT TCC GAG CGG TCG 492 Gly Pro Arg Leu Gly Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Ser 45 50 55 CAA CCT CGT GGA AGG CGA CAA CCT ATC CCC AAG GCT CGC CAG CCC GAG 540 Gln Pro Arg Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Gln Pro Glu 60 65 70 GGC AGG GCC TGG GCT CAG CCC GGG TAC CCT TGG CCC CCC TAT GGC AAT 588 Gly Arg Ala Trp Ala Gln Pro Gly Tyr Pro Trp Pro Pro Tyr Gly Asn 75 80 85 GAG GGT CTG GGG TGG GCA GGA TGG CTC CTG TCA CCC CGT GGC TCC CGG 636 Glu Gly Leu Gly Trp Ala Gly Trp Leu Leu Ser Pro Arg Gly Ser Arg 90 95 100 CCT AGT TGG GGC CCC ACG GAC CCC CGG CGT AGG TCG CGT AAT TTG GGT 684 Pro Ser Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Asn Leu Gly 105 110 115 120 AAG GTC ATC GAT ACC CTC ACA TGC GGC TTC GCC GAC CTC ATG GGG TAC 732 Lys Val Ile Asp Thr Leu Thr Cys Gly Phe Ala Asp Leu Met Gly Tyr 125 130 135 ATT CCG CTC GTC GGC GCC CCC CTA GGA GGC GCT GCC AGG GCC CTG GCG 780 Ile Pro Leu Val Gly Ala Pro Leu Gly Gly Ala Ala Arg Ala Leu Ala 140 145 150 CAT GGC GTC CGG GTT CTG GAG GAC GGC GTG AAC TAC GCA ACA GGG AAT 828 His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 155 160 165 CTG CCC GGT TGC TCT TTC TCT ATC TTC CTC TTG GCT CTG CTG TCT TGT 876 Leu Pro Gly Cys Ser Phe Ser Ile Phe Leu Leu Ala Leu Leu Ser Cys 170 175 180 TTG ACC ATC CCA GCA TCC GCT TAT GAA GTG CGC AAC GTG TCC GGG TTG 924 Leu Thr Ile Pro Ala Ser Ala Tyr Glu Val Arg Asn Val Ser Gly Leu 185 190 195 200 TAC CAT GTC ACG AAC GAC TGC TCC AAC TCA AGT ATT GTG TAT GAG GCA 972 Tyr His Val Thr Asn Asp Cys Ser Asn Ser Ser Ile Val Tyr Glu Ala 205 210 215 GCG GAC TTG ATC ATG CAC ACC CCT GGG TGC GTG CCC TGC GTC CTG GAG 1020 Ala Asp Leu Ile Met His Thr Pro Gly Cys Val Pro Cys Val Leu Glu 220 225 230 GGA AAC TCC TCC CGT TGC TGG GTA GCG CTC ACT CTC ACG CTC GCG GCC 1068 Gly Asn Ser Ser Arg Cys Trp Val Ala Leu Thr Leu Thr Leu Ala Ala 235 240 245 AGG AAC AGC AGC ATC CCC ACT ACG ACA ATA CGA CGT CAC GTC GAC TTG 1116 Arg Asn Ser Ser Ile Pro Thr Thr Thr Ile Arg Arg His Val Asp Leu 250 255 260 CTC GTT GGG GCG GCT GCT TTC TGC TCC GCT ATG TAC GTG GGG GAT CTC 1164 Leu Val Gly Ala Ala Ala Phe Cys Ser Ala Met Tyr Val Gly Asp Leu 265 270 275 280 TGC GGA TCT GTT TTC CTC GTC TCC CAG CTA TTC ACC TTT TCA CCT CGC 1212 Cys Gly Ser Val Phe Leu Val Ser Gln Leu Phe Thr Phe Ser Pro Arg 285 290 295 CGG TAT GAG ACG GTA CAA GAC TGC AAT TGC TCA CTC TAT TCC GGC CAC 1260 Arg Tyr Glu Thr Val Gln Asp Cys Asn Cys Ser Leu Tyr Ser Gly His 300 305 310 GTA TCA GGT CAC CGC ATG GCC TGG GAT ATG ATG ATG AAC TGG TCG CCC 1308 Val Ser Gly His Arg Met Ala Trp Asp Met Met Met Asn Trp Ser Pro 315 320 325 ACA ACA GCC CTA GTG GTA TCG CAG CTA CTC CGG ATC CCA CAA GCC GTC 1356 Thr Thr Ala Leu Val Val Ser Gln Leu Leu Arg Ile Pro Gln Ala Val 330 335 340 GTG GAC ATG GTG GCG GGG GCC CAC TGG GGA GTC TTG GCA GGC CTC GCC 1404 Val Asp Met Val Ala Gly Ala His Trp Gly Val Leu Ala Gly Leu Ala 345 350 355 360 TAC TAT TCC ATG GTG GGG AAC TGG GCT AAG GTC TTG ATT GTG ATG CTA 1452 Tyr Tyr Ser Met Val Gly Asn Trp Ala Lys Val Leu Ile Val Met Leu 365 370 375 CTC TTT GCC GGC GTC GAC GGG AGA ACC CAA GTG ACG GGA GCA CAG GCA 1500 Leu Phe Ala Gly Val Asp Gly Arg Thr Gln Val Thr Gly Ala Gln Ala 380 385 390 GGC CAC ACC ACC AGT GGC CTT GCG TCC CTC TTC ACA CCA GGG CCG TCT 1548 Gly His Thr Thr Ser Gly Leu Ala Ser Leu Phe Thr Pro Gly Pro Ser 395 400 405 CAG AAA ATC CAG CTT GTA AAC TCT AAC GGC AGT CGG CAC ATC AAC AGA 1596 Gln Lys Ile Gln Leu Val Asn Ser Asn Gly Ser Arg His Ile Asn Arg 410 415 420 ACT GCC CTG AGT TGC AAC GAC TCC CTC AAA ACT GGC TTC CTT GCC GCG 1644 Thr Ala Leu Ser Cys Asn Asp Ser Leu Lys Thr Gly Phe Leu Ala Ala 425 430 435 440 CTG TTC TAC ACA CAT AAG TTC AAC GCG GCT GGA TGC CCG GAG CGC ATG 1692 Leu Phe Tyr Thr His Lys Phe Asn Ala Ala Gly Cys Pro Glu Arg Met 445 450 455 GCC AGC TGC TGC TCT ATT GAC ACG TTC GAC CAA GGG TGG GGT 1734 Ala Ser Cys Cys Ser Ile Asp Thr Phe Asp Gln Gly Trp Gly 460 465 470 配列番号：５ 配列の長さ：１７３４ 配列の型：核酸 鎖の数：二本鎖 トポロジー：直鎖状 配列の種類：cDNA to genomic RNA ハイポセティカル：Ｎｏ アンチセンス：Ｎｏ 起源 生物名：Hepatitis C Virus 単離クローン名：JK2-B 組織の種類：肝臓細胞 GGCGACACTC CACCATAGAT CACTCCCCTG TGAGGAACTG CTGTCTTCAC GACGAAAGCG 60 TCTAGCCATG GCGTTAGTAT GACTGTCGTG CAGCCTGCAG GACCCCCCCT CCCGGGAGAG 120 CCATAGTGGT CTGCGGAACC GGTGAGTACA CCGGAATTGC CAGGACGACC GGGTCCTTTC 180 TTGGATCACC CCGCTCAATG CCTGGAGATT TGGGCGTGCC CCCGCGAGAC TGCTAGCCGA 240 GTAGTGTTGG GTCGCGAAAG GCCTTGTGGT ACTGCCTGAT AGGGTGCTTG CGAGTGCCCC 300 GGGAGGTCTC GTAGACCGTG CATC ATG AGC ACA AAT CCT AAA CCT CAA 348 Met Ser Thr Asn Pro Lys Pro Gln 1 5 AGA AAA ACC AAA CGT AAC ACC AAC CGC CGC CCA CAG GAC GTC AAG TTC 396 Arg Lys Thr Lys Arg Asn Thr Asn Arg Arg Pro Gln Asp Val Lys Phe 10 15 20 CCG GGC GGT GGC CAG ATC GTT GGT GGA GTT TAC CTG TTG CCG CGC AGG 444 Pro Gly Gly Gly Gln Ile Val Gly Gly Val Tyr Leu Leu Pro Arg Arg 25 30 35 40 GGC CCC AGG TTG GGT GTG CGC GCG ACT AGG AAG ACT TCC GAG CGG TCG 492 Gly Pro Arg Leu Gly Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Ser 45 50 55 CAA CCT CGT GGA AGG CGA CAA CCT ATC CCC AAG GCT CGC CAG CCC GAG 540 Gln Pro Arg Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Gln Pro Glu 60 65 70 GGC AGG GCC TGG GCT CAG CCC GGG TAC CCT TGG CCC CTC TAT GGC AAT 588 Gly Arg Ala Trp Ala Gln Pro Gly Tyr Pro Trp Pro Leu Tyr Gly Asn 75 80 85 GAG GGT CTG GGG TGG GCA GGA TGG CTC CTG TCA CCC CGT GGC TCC CGG 636 Glu Gly Leu Gly Trp Ala Gly Trp Leu Leu Ser Pro Arg Gly Ser Arg 90 95 100 CCT AGT TGG GGC CCC ACG GAC CCC CGG CGT AGG TCG CGT AAT TTG GGT 684 Pro Ser Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Asn Leu Gly 105 110 115 120 AAG GTC ATC GAT ACC CTC ACA TGC GGC TTC GCC GAC CTC ATG GGG TAC 732 Lys Val Ile Asp Thr Leu Thr Cys Gly Phe Ala Asp Leu Met Gly Tyr 125 130 135 ATT CCG CTC GTC GGC GCC CCC CTA GGA GGC GCT GCC AGG GCC CTG GCG 780 Ile Pro Leu Val Gly Ala Pro Leu Gly Gly Ala Ala Arg Ala Leu Ala 140 145 150 CAT GGC GTC CGG GTT CTG GAG GAC GGC GTG AAC TAC GCA ACA GGG AAT 828 His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 155 160 165 CTG CCC GGT TGC TCT TTC TCT ATC TTC CTC TTG GCT CTG CTG TCT TGT 876 Leu Pro Gly Cys Ser Phe Ser Ile Phe Leu Leu Ala Leu Leu Ser Cys 170 175 180 TTG ACC ATC CCA GCA TCC GCT TAT GAA GTG CGC AAC GTG TCC GGG TTG 924 Leu Thr Ile Pro Ala Ser Ala Tyr Glu Val Arg Asn Val Ser Gly Leu 185 190 195 200 TAC CAT GTC ACG AAC GAC TGC TCC AAC TCA AGT ATT GTG TAT GAG GCA 972 Tyr His Val Thr Asn Asp Cys Ser Asn Ser Ser Ile Val Tyr Glu Ala 205 210 215 GCG GAC TTG ATC ATG CAC ACC CCT GGG TGC GTG CCC TGC GTC CTG GAG 1020 Ala Asp Leu Ile Met His Thr Pro Gly Cys Val Pro Cys Val Leu Glu 220 225 230 GGA AAC TCC TCC CGC TGC TGG GTA GCG CTC ACT CTC ACG CTC GCG GCC 1068 Gly Asn Ser Ser Arg Cys Trp Val Ala Leu Thr Leu Thr Leu Ala Ala 235 240 245 AGG AAC AAC AGC ATC CCC ACT ACG ACA ATA CGA CGT CAT GTC GAC TTG 1116 Arg Asn Asn Ser Ile Pro Thr Thr Thr Ile Arg Arg His Val Asp Leu 250 255 260 CTC GTT GGG GCG GCT GCT TTC TGC TCC GCT ATG TAC GTG GGG GAT CTC 1164 Leu Val Gly Ala Ala Ala Phe Cys Ser Ala Met Tyr Val Gly Asp Leu 265 270 275 280 TGC GGA CCT GTT TTC CTC GTC TCC CAG CTA TTC ACC TTC TCA CCT CGC 1212 Cys Gly Pro Val Phe Leu Val Ser Gln Leu Phe Thr Phe Ser Pro Arg 285 290 295 CGG TAT GAG ACG GTA CAA GAC TGC AAC TGC TCA CTC TAT TCC GGC CAC 1260 Arg Tyr Glu Thr Val Gln Asp Cys Asn Cys Ser Leu Tyr Ser Gly His 300 305 310 GTA TCA GGT CAC CGC ATG GCC TGG GAT ATG ATG ATG AAC TGG TCG CCC 1308 Val Ser Gly His Arg Met Ala Trp Asp Met Met Met Asn Trp Ser Pro 315 320 325 ACA ACA GCC CTA GTG GTA TCG CAG TTA CTC CGG ATC CCA CAA GCC GTC 1356 Thr Thr Ala Leu Val Val Ser Gln Leu Leu Arg Ile Pro Gln Ala Val 330 335 340 GTG GAC ATG GTG GCG GGG GCC CAC TGG GGA GTC TTG GCG GGC CTC GCC 1404 Val Asp Met Val Ala Gly Ala His Trp Gly Val Leu Ala Gly Leu Ala 345 350 355 360 TAC TAT TCC ATG GTG GGG AAC TGG GCT AAG GTC TTG ATT GTG ATG CTA 1452 Tyr Tyr Ser Met Val Gly Asn Trp Ala Lys Val Leu Ile Val Met Leu 365 370 375 CTC TTT GCC GGC GTC GAC GGG CAG ACC CGA GTG ACG GGG GCA CAA GTA 1500 Leu Phe Ala Gly Val Asp Gly Gln Thr Arg Val Thr Gly Ala Gln Val 380 385 390 GGC CAG ACC ACC AGT AGC CTT ACG TCC CTC TTC ACA CCA GGG CCG TCT 1548 Gly Gln Thr Thr Ser Ser Leu Thr Ser Leu Phe Thr Pro Gly Pro Ser 395 400 405 CAG AAC ATC CAG CTT GTA AAC TCT AAC GGC AGT CGG CAC ATC AAC AGA 1596 Gln Asn Ile Gln Leu Val Asn Ser Asn Gly Ser Arg His Ile Asn Arg 410 415 420 ACT GCC CTG AGT TGC AAC GAC TCC CTC AAA ACT GGC TTC CTT GCC GCG 1644 Thr Ala Leu Ser Cys Asn Asp Ser Leu Lys Thr Gly Phe Leu Ala Ala 425 430 435 440 CTG TTC TAC ACA CAT AAG TTC AAC GCG GCT GGA TGC CCG GAG CGC ATG 1692 Leu Phe Tyr Thr His Lys Phe Asn Ala Ala Gly Cys Pro Glu Arg Met 445 450 455 GCC AGC TGC CGC TCC ATT GAC ACG TTC GAC CAA GGG TGG GGT 1734 Ala Ser Cys Arg Ser Ile Asp Thr Phe Asp Gln Gly Trp Gly 460 465 470 配列番号：６ 配列の長さ：１７３４ 配列の型：核酸 鎖の数：二本鎖 トポロジー：直鎖状 配列の種類：cDNA to genomic RNA ハイポセティカル：Ｎｏ アンチセンス：Ｎｏ 起源 生物名：Hepatitis C Virus 単離クローン名：JK2-C 組織の種類：肝臓細胞 GGCGACACTC CACCATAGAT CACTCCCCTG TGAGGAACTG CTGTCCTCAC GACGAAAGCG 60 TCTAGCCATG GCGTTAGTAT GACTGTCGTG CAGCCTGCAG GACCCCCCCT CCCGGGAGAG 120 CCATAGTGGT CTGCGGAACC GGTGAGTACA CCGGAATTGC CAGGACGACC GGGTCCTTTC 180 TTGGATCACC CCGCTCAATG CCTGGAGATT TGGGCGTGCC CCCGCGAGAC TGCTAGCCGA 240 GTAGTGTTGG GTCGCGAAAG GCCTTGTGGT ACTGCCTGAT AGGGTGCTTG CGAGTGCCCC 300 GGGAGGTCTC GTAGACCGTG CATC ATG AGC ACA AAT CCT AAA CCT CAA 348 Met Ser Thr Asn Pro Lys Pro Gln 1 5 AGA AAA ACC AAA CGT AAC ACC AAC CGC CGC CCA CAG GAC GTC AAG TTC 396 Arg Lys Thr Lys Arg Asn Thr Asn Arg Arg Pro Gln Asp Val Lys Phe 10 15 20 CCG GGC GGT GGC CAG ATC GTT GGT GGA GTT TAC CTG TTG CCG CGC AGG 444 Pro Gly Gly Gly Gln Ile Val Gly Gly Val Tyr Leu Leu Pro Arg Arg 25 30 35 40 GGC CCC AGG TTG GGT GTG CGC GCG ACT AGG AAG ACT TCC GAG CGG TCG 492 Gly Pro Arg Leu Gly Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Ser 45 50 55 CAA CCT CGT GGA AGG CGA CAA CCT ATC CCC AAG GCT CGC CAG CCC GAG 540 Gln Pro Arg Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Gln Pro Glu 60 65 70 GGC AGG GCC TGG GCT CAG CCC GGG TAC CCT TGG CCC CCC TAT GGC AAT 588 Gly Arg Ala Trp Ala Gln Pro Gly Tyr Pro Trp Pro Pro Tyr Gly Asn 75 80 85 GAG GGT CTG GGG TGG GCA GGA TGG CTC CTG TCA CCC CGT GGC TCC CGG 636 Glu Gly Leu Gly Trp Ala Gly Trp Leu Leu Ser Pro Arg Gly Ser Arg 90 95 100 CCT AGT TGG GGC CCC ACG GAC CCC CGG CGT AGG TCG CGT AAT TTG GGT 684 Pro Ser Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Asn Leu Gly 105 110 115 120 AAG GTC ATC GAT ACC CTC ACA TGC GGC TTC GCC GAC CTC ATG GGG TAC 732 Lys Val Ile Asp Thr Leu Thr Cys Gly Phe Ala Asp Leu Met Gly Tyr 125 130 135 ATT CCG CTC GTC GGC GCC CCC CTA GGA GGC GCT GCC AGG GCC CTG GCG 780 Ile Pro Leu Val Gly Ala Pro Leu Gly Gly Ala Ala Arg Ala Leu Ala 140 145 150 CAT GGC GTC CGG GTT CTG GAG GAC GGC GTG AAC TAC GCA ACA GGG AAT 828 His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 155 160 165 CTG CCC GGT TGC TCT TTC TCT ATC TTC CTC TTG GCT CTG CTG TCT TGT 876 Leu Pro Gly Cys Ser Phe Ser Ile Phe Leu Leu Ala Leu Leu Ser Cys 170 175 180 TTG ACC ATC CCA GCA TCC GCT TAT GAA GTG CGC AAC GTG TCC GGG TTG 924 Leu Thr Ile Pro Ala Ser Ala Tyr Glu Val Arg Asn Val Ser Gly Leu 185 190 195 200 TAC CAT GTC ACG AAC GAC TGC TCC AAC ACA AGT ATT GTG TAT GAG GCA 972 Tyr His Val Thr Asn Asp Cys Ser Asn Thr Ser Ile Val Tyr Glu Ala 205 210 215 GCG GAC TTG ATC ATG CAC ACC CCT GGG TGC GTG CCC TGC GTC CTG GAG 1020 Ala Asp Leu Ile Met His Thr Pro Gly Cys Val Pro Cys Val Leu Glu 220 225 230 GGA AAC TCC TCC CGC TGC TGG GTA GCG CTC ACT CTC ACG CTC GCG GCC 1068 Gly Asn Ser Ser Arg Cys Trp Val Ala Leu Thr Leu Thr Leu Ala Ala 235 240 245 AGG AAC TAC AGC ATC CCC ACT ACG ACA ATA CGA CGT CAT GTC GAC TTG 1116 Arg Asn Tyr Ser Ile Pro Thr Thr Thr Ile Arg Arg His Val Asp Leu 250 255 260 CTC GTT GGG GCG GCT GCT TTC TGC TCC GCT ATG TAC GTG GGG GAT CTC 1164 Leu Val Gly Ala Ala Ala Phe Cys Ser Ala Met Tyr Val Gly Asp Leu 265 270 275 280 TGC GGA TCT GTT TTC CTC GTC TCC CAG CTA TTT ACC TCC TCA CCT CGC 1212 Cys Gly Ser Val Phe Leu Val Ser Gln Leu Phe Thr Ser Ser Pro Arg 285 290 295 CGG TAT GAG ACG GTA CAA GAC TGC AAT TGC TCA CTC TAT TCC GGC CAC 1260 Arg Tyr Glu Thr Val Gln Asp Cys Asn Cys Ser Leu Tyr Ser Gly His 300 305 310 GTA TCA GGT CAC CGC ATG GCC TGG GAT ATG ATG ATG AAC TGG TCG CCC 1308 Val Ser Gly His Arg Met Ala Trp Asp Met Met Met Asn Trp Ser Pro 315 320 325 ACA ACA GCC CTA GTG GTA TCG CAG TTA CTC CGG ATC CCA CAA GCC GTC 1356 Thr Thr Ala Leu Val Val Ser Gln Leu Leu Arg Ile Pro Gln Ala Val 330 335 340 GTG GAC ATG GTG GCG GGG GCC CAC TGG GGA GTC TTG GCG GGC CTC GCC 1404 Val Asp Met Val Ala Gly Ala His Trp Gly Val Leu Ala Gly Leu Ala 345 350 355 360 TAC TAT TCC ATG GTG GGG AAC TGG GCT AAG GTC TTG ATT GTG ATG CTA 1452 Tyr Tyr Ser Met Val Gly Asn Trp Ala Lys Val Leu Ile Val Met Leu 365 370 375 CTC TTT GCC GGC GTT GAC GGG ACA ACC CAT GTG ACG GGG GCA CAG ATG 1500 Leu Phe Ala Gly Val Asp Gly Thr Thr His Val Thr Gly Ala Gln Met 380 385 390 GGC CAG ACC ACC AGT GGC CTT ACG TCC CTC TTC ACA CCA GGG CCG TCT 1548 Gly Gln Thr Thr Ser Gly Leu Thr Ser Leu Phe Thr Pro Gly Pro Ser 395 400 405 CAG AAC ATC CAG CTT GTA AAC TCT AAC GGC AGT CGG CAC ATC AAC AGA 1596 Gln Asn Ile Gln Leu Val Asn Ser Asn Gly Ser Arg His Ile Asn Arg 410 415 420 ACT GCC CTG AGT TGC AAC GAC TCC CTC AAA ACT GGG TTC CTT GCC GCG 1644 Thr Ala Leu Ser Cys Asn Asp Ser Leu Lys Thr Gly Phe Leu Ala Ala 425 430 435 440 CTG TTC TAC ACA CAT AAG TTC AAC GCG GCT GGA TGC CCG GAG CGC ATG 1692 Leu Phe Tyr Thr His Lys Phe Asn Ala Ala Gly Cys Pro Glu Arg Met 445 450 455 GCC AGC TGC CGC TCC ATT GAC ACG TTC GAC CAG GGG TGG GGT 1734 Ala Ser Cys Arg Ser Ile Asp Thr Phe Asp Gln Gly Trp Gly 460 465 470 配列番号：７ 配列の長さ：１７３４ 配列の型：核酸 鎖の数：二本鎖 トポロジー：直鎖状 配列の種類：cDNA to genomic RNA ハイポセティカル：Ｎｏ アンチセンス：Ｎｏ 起源 生物名：Hepatitis C Virus 単離クローン名：JK3-A 組織の種類：肝臓細胞 GGCGACACTC CACCATAGAT CACTCCCCTG TGAGGAACTG CTGTCTTCAC GACGAAAGCG 60 TCTGGCCATG GCGTTAGTAT GACTGTCGTG CAGCCTGCAG GCCCCCCCCT CCCGGGAGAG 120 CCATAGTGGT CTGCGGAACC GGTGAGTACA CCGGAATTGC CAGGACGACC GGGTCCTTTC 180 TTGGATCACC CCGCTCAATG CCTGGAGATT TGGGCGTGCC CCCGCGAGAC TGCTAGCCGA 240 GTAGTGTTGG GTCGCGAAAG GCCTTGTGGT ACTGCCTGAT AGGGTGCTTG CGAGTGCCCC 300 GGGAGGTCTC GTAGACCGTG CAAC ATG AGC ACA AAT CCT AAA CCT CAA 348 Met Ser Thr Asn Pro Lys Pro Gln 1 5 AGA CAA ACC AAA CGC AAC ACC ATC CGC CGC CCA CAG GAC GTT AAG TTC 396 Arg Gln Thr Lys Arg Asn Thr Ile Arg Arg Pro Gln Asp Val Lys Phe 10 15 20 CCG GGC GGC GGT CAG ATC GTT GGT GGA GTT TAC CTG TTG CCG CGC AGG 444 Pro Gly Gly Gly Gln Ile Val Gly Gly Val Tyr Leu Leu Pro Arg Arg 25 30 35 40 GGC CCC AGG TTG GGT GTG CGC GCG ACT AGG AAG ACT TCC GAG CGG TCG 492 Gly Pro Arg Leu Gly Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Ser 45 50 55 CAA CCT CGT GGA AGG CGA CAA CCT ATC CCC AAG GCT CGC CAG CCC GAG 540 Gln Pro Arg Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Gln Pro Glu 60 65 70 GGT AGG GCC TGG GCT CAG CCC GGG TAC CCT TGG CCC CTC TAC GGC AAT 588 Gly Arg Ala Trp Ala Gln Pro Gly Tyr Pro Trp Pro Leu Tyr Gly Asn 75 80 85 GAG GGC ATG GGA TGG ACG GGA TGG CTC TTG TCC CCC CGT GGT TCT CGG 636 Glu Gly Met Gly Trp Thr Gly Trp Leu Leu Ser Pro Arg Gly Ser Arg 90 95 100 CCT AGT TGG GGC CCC ACG GAC CCC CGG CGT AGG TCG CGT AAT TTG GGT 684 Pro Ser Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Asn Leu Gly 105 110 115 120 AAG GTC ATC GAT ACC CTC ACA TGC GGC TTC GCC GAC CTC ATG GGG TAC 732 Lys Val Ile Asp Thr Leu Thr Cys Gly Phe Ala Asp Leu Met Gly Tyr 125 130 135 ATT CCG CTC GTC GGC GCC CCC CTA GGG GGC GTT GCC AGG GCC CTG GCG 780 Ile Pro Leu Val Gly Ala Pro Leu Gly Gly Val Ala Arg Ala Leu Ala 140 145 150 CAT GGC GTC CGG GTT CTG GAG GAC GGC GTG AAT TAC GCA ACA GGG AAT 828 His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 155 160 165 CTG CCC GGT TGC TCT TTC TCT ATC TTC CCC TTG GCT TTG CTG TCC TGT 876 Leu Pro Gly Cys Ser Phe Ser Ile Phe Pro Leu Ala Leu Leu Phe Cys 170 175 180 CTG ACC GTC CCA GCT TCC GCT TAT GAA GTG CGC AAC GTG TCC GGG GCG 924 Leu Thr Val Pro Ala Ser Ala Tyr Glu Val Arg Asn Val Ser Gly Ala 185 190 195 200 TAC CGT GTC ACG AAC GAC TGC TCC AAC ACA AGT ATT GTG TAT GAG GCA 972 Tyr Arg Val Thr Asn Asp Cys Ser Asn Thr Ser Ile Val Tyr Glu Ala 205 210 215 GCG GAC ATG ATC ATG CAC ACC CCC GGG TGC GTG CCC TGC GTC CGG GAC 1020 Ala Asp Met Ile Met His Thr Pro Gly Cys Val Pro Cys Val Arg Asp 220 225 230 GGA AAC ATC TCC CGC TGC TGG GTA GCG CTC ACT CTC ACG CTC GCG GCC 1068 Gly Asn Ile Ser Arg Cys Trp Val Ala Leu Thr Leu Thr Leu Ala Ala 235 240 245 AGG AAC AGC AGC ATC CCC ACT ACG ACA ATA CGA CGT CAT GTC GAT TTG 1116 Arg Asn Ser Ser Ile Pro Thr Thr Thr Ile Arg Arg His Val Asp Leu 250 255 260 CTC GTT GGG GCA GCT GCC CTC TGC TCC GCT ATG TAC GTG GGA GAT CTC 1164 Leu Val Gly Ala Ala Ala Leu Cys Ser Ala Met Tyr Val Gly Asp Leu 265 270 275 280 TGC GGA TCT GTC TTC CTC GTC TCC CAG TTG TTC ACC TTC TCA CCA CGC 1212 Cys Gly Ser Val Phe Leu Val Ser Gln Leu Phe Thr Phe Ser Pro Arg 285 290 295 CGA TAC GAG ACG GTA CAG GAC TGC AAT TGC TCA ATC TAT CCC GGC AAA 1260 Arg Tyr Glu Thr Val Gln Asp Cys Asn Cys Ser Ile Tyr Pro Gly Lys 300 305 310 TTA ACA GGT CAC CGC ATG GCC TGG GAT ATG ATG ATG AAC TGG TCG CCC 1308 Leu Thr Gly His Arg Met Ala Trp Asp Met Met Met Asn Trp Ser Pro 315 320 325 ACA ACA GCC CTA GTG GTA TCG CAG TTA CTC CGG ATC CCA CAA GCC GTC 1356 Thr Thr Ala Leu Val Val Ser Gln Leu Leu Arg Ile Pro Gln Ala Val 330 335 340 GCG GAC ATG GTG GCG GGG GCC CAC TGG GGA GTC TTG GCG GGC CTC GCC 1404 Ala Asp Met Val Ala Gly Ala His Trp Gly Val Leu Ala Gly Leu Ala 345 350 355 360 TAC TAT TCC ATG GTG GGG AAC TGG GCT AAG GTC TTG ATT GTG ATG CTA 1452 Tyr Tyr Ser Met Val Gly Asn Trp Ala Lys Val Leu Ile Val Met Leu 365 370 375 CTC TTT GCC GGC GTC GAC GGG CAG ACC CGC GTG ACG GGG GCA CAA GTA 1500 Leu Phe Ala Gly Val Asp Gly Gln Thr Arg Val Thr Gly Ala Gln Val 380 385 390 GGC CGC ACC ACC AGT AGC CTT ACG TCC CTC TTC ACA CCA GGG CCG TCT 1548 Gly Arg Thr Thr Ser Ser Leu Thr Ser Leu Phe Thr Pro Gly Pro Ser 395 400 405 CAG AAC ATC CAG CTT GTA AAC TCT AAC GGC AGC TGG CAC ATC AAC AGA 1596 Gln Asn Ile Gln Leu Val Asn Ser Asn Gly Ser Trp His Ile Asn Arg 410 415 420 ACT GCC CTG AGT TGC AAC GAC TCC CTC AAA ACT GGG TTC CTT GCC GCG 1644 Thr Ala Leu Ser Cys Asn Asp Ser Leu Lys Thr Gly Phe Leu Ala Ala 425 430 435 440 CTG TTC TAC ACA CAT AAG TTC AAC GCG TCC GGA TGC CCG GAG CGC ATG 1692 Leu Phe Tyr Thr His Lys Phe Asn Ala Ser Gly Cys Pro Glu Arg Met 445 450 455 GCC AGC TGC CGC TCC ATT GAC ACG TTC GAC CAG GGG TGG GGT 1734 Ala Ser Cys Arg Ser Ile Asp Thr Phe Asp Gln Gly Trp Gly 460 465 470 配列番号：８ 配列の長さ：１７３４ 配列の型：核酸 鎖の数：二本鎖 トポロジー：直鎖状 配列の種類：cDNA to genomic RNA ハイポセティカル：Ｎｏ アンチセンス：Ｎｏ 起源 生物名：Hepatitis C Virus 単離クローン名：JK3-B 組織の種類：肝臓細胞 GGCGACACTC CACCATAGAT CACTCCCCTG TGAGGAACTG CTGTCTTCAC GACGAAAGCG 60 TCTAGCCATG GCGTTAGTAT GACTGTCGTG CAGCCTGCAG GCCCCCCCCT CCCGGGAGAG 120 CCATAGTGGT CTGCGGAACC GGTGAGTACA CCGGAATTGC CAGGACGACC GGGTCCTTTC 180 TTGGATCACC CCGCTCAATG CCTGGAGATT TGGGCGTGCC CCCGCGAGAC TGCTAGCCGA 240 GTAGTGTTGG GTCGCGAAAG GCCTTGTGGT ACTGCCTGAT AGGGTGCTTG CGAGTGCCCC 300 GGGAGGTCTC GTAGACCGTG CAAC ATG AGC ACA AAT CCT AAA CCT CAA 348 Met Ser Thr Asn Pro Lys Pro Gln 1 5 AGA CAA ACC AAA CGC AAC ACC ATC CGC CGC CCA CAG GAC GTT AAG TTC 396 Arg Gln Thr Lys Arg Asn Thr Ile Arg Arg Pro Gln Asp Val Lys Phe 10 15 20 CCG GGC GGC GGT CAG ATC GTT GGT GGA GTT TAC CTG TTG CCG CGC AGG 444 Pro Gly Gly Gly Gln Ile Val Gly Gly Val Tyr Leu Leu Pro Arg Arg 25 30 35 40 GGC CCC AGG TTG GGT GTG CGC GCG ACT AGG AAG ACT TCC GAG CGG TCG 492 Gly Pro Arg Leu Gly Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Ser 45 50 55 CAA CCT CGT GGA AGG CGA CAA CCT ATC CCC AAG GCT CGC CAG CCC GAG 540 Gln Pro Arg Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Gln Pro Glu 60 65 70 GGT AGG GCC TGG GCT CAG CCC GGG TAC CCT TGG CCC CTC TAC GGC AAT 588 Gly Arg Ala Trp Ala Gln Pro Gly Tyr Pro Trp Pro Leu Tyr Gly Asn 75 80 85 GAG GGC ATA GGA TGG ACG GGA TGG CTC TTG TCC CCC CGT GGT TCT CGG 636 Glu Gly Ile Gly Trp Thr Gly Trp Leu Leu Ser Pro Arg Gly Ser Arg 90 95 100 CCT AGT TGG GGC CCC ACG GAC CCC CGG CGT AGG TCG CGT AAT TTG GGT 684 Pro Ser Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Asn Leu Gly 105 110 115 120 AAG GTC ATC GAT ACC CTC ACA TGC GGC TTC GCC GAC CTC ATG GGG TAC 732 Lys Val Ile Asp Thr Leu Thr Cys Gly Phe Ala Asp Leu Met Gly Tyr 125 130 135 ATT CCG CTC GTC GGC GCC CCC CTA GGG GGC GTT GCC AGG GCC CTG GCG 780 Ile Pro Leu Val Gly Ala Pro Leu Gly Gly Val Ala Arg Ala Leu Ala 140 145 150 CAT GGC GTC CGG GTT CTG GAG GAC GGC GTG AAT TAC GCA ACA GGG AAT 828 His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 155 160 165 CTG CCC GGT TGC TCT TTC TCT ATC TTC CTC TTG GCT TTG CTG TCC TGT 876 Leu Pro Gly Cys Ser Phe Ser Ile Phe Leu Leu Ala Leu Leu Phe Cys 170 175 180 CTG ACC GTC CCA GCT TCC GCT TAT GAA GTG CGC AAC GTG TCC GGG GCG 924 Leu Thr Val Pro Ala Ser Ala Tyr Glu Val Arg Asn Val Ser Gly Ala 185 190 195 200 TAC CAT GTC ACG AAC GAC TGC TCC AAC ACA AGT ATC GTG TAT GAG GCA 972 Tyr His Val Thr Asn Asp Cys Ser Asn Thr Ser Ile Val Tyr Glu Ala 205 210 215 GCG GAC ATG ATC ATG CAC ACC CCC GGG TGC GTG CCC TGC GTC CGG GAC 1020 Ala Asp Met Ile Met His Thr Pro Gly Cys Val Pro Cys Val Arg Asp 220 225 230 GGA AAC ATC TCC CGC TGC TGG GTA GCG CTC ACT CTC ACG CTC GCG GCC 1068 Gly Asn Ile Ser Arg Cys Trp Val Ala Leu Thr Leu Thr Leu Ala Ala 235 240 245 AGG AAC AGC AGC ATC CCC ACT ACG ACA ATA CGA CGT CAT GTC GAT TTG 1116 Arg Asn Ser Ser Ile Pro Thr Thr Thr Ile Arg Arg His Val Asp Leu 250 255 260 CTC GTT GGG GCA GCT GCC CTC TGC TCC GCT ATG TAC GTG GGA GAT CTC 1164 Leu Val Gly Ala Ala Ala Leu Cys Ser Ala Met Tyr Val Gly Asp Leu 265 270 275 280 TGC GGA TCT GTC TTC CTC GTC TCC CAG TTG TTC ACC TTC TCA CCA CGC 1212 Cys Gly Ser Val Phe Leu Val Ser Gln Leu Phe Thr Phe Ser Pro Arg 285 290 295 CGA TAC GAG ACG GTA CAG GAC TGC AAT TGT TCA ATC TAT CCC GGC AAA 1260 Arg Tyr Glu Thr Val Gln Asp Cys Asn Cys Ser Ile Tyr Pro Gly Lys 300 305 310 TTA ACA GGT CAC CGC ATG GCC TGG GAT ATG ATG ATG AAC TGG TCG CCC 1308 Leu Thr Gly His Arg Met Ala Trp Asp Met Met Met Asn Trp Ser Pro 315 320 325 ACA ACA GCC CTA GTG GTA TCG CAG TTA CTC CGG ATC CCA CAA GCC GTC 1356 Thr Thr Ala Leu Val Val Ser Gln Leu Leu Arg Ile Pro Gln Ala Val 330 335 340 GTG GAC ATG GTG GCG GGG GCC CAC TGG GGA GTC TTG GCG GGC CTC GCC 1404 Val Asp Met Val Ala Gly Ala His Trp Gly Val Leu Ala Gly Leu Ala 345 350 355 360 TAC TAT TCC ATG GTG GGG AAC TGG GCT AAG GTC TTG ATT GTG ATG CTA 1452 Tyr Tyr Ser Met Val Gly Asn Trp Ala Lys Val Leu Ile Val Met Leu 365 370 375 CTC TTT GCC GGC GTC GAC GGG CAG ACC CGC GTG ACG GGG GCA CAA GTA 1500 Leu Phe Ala Gly Val Asp Gly Gln Thr Arg Val Thr Gly Ala Gln Val 380 385 390 GGC CGC ACC ACC AGT AGC CTT ACG TCC CTC TTC ACA CCA GGG CCG TCT 1548 Gly Arg Thr Thr Ser Ser Leu Thr Ser Leu Phe Thr Pro Gly Pro Ser 395 400 405 CAG AAC ATC CAG CTT GTA AAC TCT AAC GGC AGC TGG CAC ATC AAC AGA 1596 Gln Asn Ile Gln Leu Val Asn Ser Asn Gly Ser Trp His Ile Asn Arg 410 415 420 ACT GCC CTG AGT TGC AAC GAC TCC CTC AAA ACT GGG TTC CTT GCC GCG 1644 Thr Ala Leu Ser Cys Asn Asp Ser Leu Lys Thr Gly Phe Leu Ala Ala 425 430 435 440 CTG TTC TAC ACA CAT AAG TTC AAC GCG TCC GGA TGC CCG GAG CGC ATG 1692 Leu Phe Tyr Thr His Lys Phe Asn Ala Ser Gly Cys Pro Glu Arg Met 445 450 455 GCC AGC TGC CGC TCC ATT GAC ACG TTC GAC CAG GGG TGG GGT 1734 Ala Ser Cys Arg Ser Ile Asp Thr Phe Asp Gln Gly Trp Gly 460 465 470 配列番号：９ 配列の長さ：１７３４ 配列の型：核酸 鎖の数：二本鎖 トポロジー：直鎖状 配列の種類：cDNA to genomic RNA ハイポセティカル：Ｎｏ アンチセンス：Ｎｏ 起源 生物名：Hepatitis C Virus 単離クローン名：JK3-C 組織の種類：肝臓細胞 GGCGACACTC CACCATAGAT CACTCCCCTG TGAGGAACTG CTGTCTTCAC GACGAAAGCG 60 TCTAGCCATG GCGTTAGTAT GACTGTCGTG CAGCCTGCAG GCCCCCCCCT CCCGGGAGAG 120 CCATAGTGGT CTGCGGAACC GGTGAGTACA CCGGAATTGC CAGGACGACC GGGTCCTTTC 180 TTGGATCACC CCGCTCAATG CCTGGAGATT TGGGCGTGCC CCCGCGAGAC TGCTAGCCGA 240 GTAGTGTTGG GTCGCGAAAG GCCTTGTGGT ACTGCCTGAT AGGGTGCTTG CGAGTGCCCC 300 GGGAGGTCTC GTAGACCGTG CAAC ATG AGC ACA AAT CCT AAA CCT CAA 348 Met Ser Thr Asn Pro Lys Pro Gln 1 5 AGA CAA ACC AAA CGC AAC ACC ATC CGC CGC CCA CAG GAC GTT AAG TTC 396 Arg Gln Thr Lys Arg Asn Thr Ile Arg Arg Pro Gln Asp Val Lys Phe 10 15 20 CCG GGC GGC GGT CAG ATC GTT GGT GGA GTT TAC CTG TTG CCG CGC AGG 444 Pro Gly Gly Gly Gln Ile Val Gly Gly Val Tyr Leu Leu Pro Arg Arg 25 30 35 40 GGC CCC AGG TTG GGT GTG CGC GCG ACT AGG AAG ACT TCC GAG CGG TCG 492 Gly Pro Arg Leu Gly Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Ser 45 50 55 CAA CCT CGT GGA AGG CGA CAA CCT ATC CCC AAG GCT CGC CAG CCC GAG 540 Gln Pro Arg Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Gln Pro Glu 60 65 70 GGT AGG GCC TGG GCT CAG CCC GGG TAC CCT TGG CCC CTC TAC GGC AAT 588 Gly Arg Ala Trp Ala Gln Pro Gly Tyr Pro Trp Pro Leu Tyr Gly Asn 75 80 85 GAG GGC ATG GGA TGG ACG GGA TGG CTC TTG TCC CCC CGT GGT TCT CGG 636 Glu Gly Met Gly Trp Thr Gly Trp Leu Leu Ser Pro Arg Gly Ser Arg 90 95 100 CCT AGT TGG GGC CCC ACG GAC CCC CGG CGT AGG TCG CGT AAT TTG GGT 684 Pro Ser Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Asn Leu Gly 105 110 115 120 AAG GTC ATC GAT ACC CTC ACA TGC GGC TTC GCC GAC CTC ATG GGG TAC 732 Lys Val Ile Asp Thr Leu Thr Cys Gly Phe Ala Asp Leu Met Gly Tyr 125 130 135 ATT CCG CTC GTC GGC GCC CCC CTA GGG GGC GTT GCC AGG GCC CTG GCG 780 Ile Pro Leu Val Gly Ala Pro Leu Gly Gly Val Ala Arg Ala Leu Ala 140 145 150 CAT GGC GTC CGG GTT CTG GAG GAC GGC GTG AAT TAC GCA ACA GGG AAT 828 His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 155 160 165 CTG CCC GGT TGC TCT TTC TCT ATC TTC CTC TTG GCT TTG CTG TCC TGT 876 Leu Pro Gly Cys Ser Phe Ser Ile Phe Leu Leu Ala Leu Leu Phe Cys 170 175 180 CTG ACC GTC CCA GCT TCC GCT TAT GAA GTG CGC AAC GTG TCC GGG GCG 924 Leu Thr Val Pro Ala Ser Ala Tyr Glu Val Arg Asn Val Ser Gly Ala 185 190 195 200 TAC CAT GTC ACG AAC GAC TGC TCC AAC TCA AGT ATC GTG TAT GAG GCA 972 Tyr His Val Thr Asn Asp Cys Ser Asn Ser Ser Ile Val Tyr Glu Ala 205 210 215 GCG GAC ATG ATC ATG CAC ACC CCC GGG TGC GTG CCC TGC GTC CGG GAC 1020 Ala Asp Met Ile Met His Thr Pro Gly Cys Val Pro Cys Val Arg Asp 220 225 230 GGA AAC ATC TCC CGC TGC TGG GTA GCG CTC ACT CTC ACG CTC GCG GCC 1068 Gly Asn Ile Ser Arg Cys Trp Val Ala Leu Thr Leu Thr Leu Ala Ala 235 240 245 AGG AAC AGC AGC ATC CCC ACT ACG ACA ATA CGA CGT CAT GTC GAT TTG 1116 Arg Asn Ser Ser Ile Pro Thr Thr Thr Ile Arg Arg His Val Asp Leu 250 255 260 CTC GTT GGG GCA GCT GCC CTC TGC TCC GCT ATG TAC GTG GGA GAT CTC 1164 Leu Val Gly Ala Ala Ala Leu Cys Ser Ala Met Tyr Val Gly Asp Leu 265 270 275 280 TGC GGA TCT GTC TTC CTC GTC TCC CAG TTG TTC ACC TTC TCA CCA CGC 1212 Cys Gly Ser Val Phe Leu Val Ser Gln Leu Phe Thr Phe Ser Pro Arg 285 290 295 CGA TAC GAG ACG GTA CAG GAC TGC AAT TGC TCA ATC TAT CCC GGC AAA 1260 Arg Tyr Glu Thr Val Gln Asp Cys Asn Cys Ser Ile Tyr Pro Gly Lys 300 305 310 TTA ACA GGT CAC CGC ATG GCT TGG GAT ATG ATG ATG AAC TGG TCG CCT 1308 Leu Thr Gly His Arg Met Ala Trp Asp Met Met Met Asn Trp Ser Pro 315 320 325 ACA ACA GCC CTA GTG GTG TCG CAG TTA CTC CGG ATC CCA CAA GCC GTC 1356 Thr Thr Ala Leu Val Val Ser Gln Leu Leu Arg Ile Pro Gln Ala Val 330 335 340 GTG GAC ATG GTA GCG GGG GCC CAC TGG GGA GTC CTG GCG GGC CTC GCC 1404 Val Asp Met Val Ala Gly Ala His Trp Gly Val Leu Ala Gly Leu Ala 345 350 355 360 TAC TAT TCC ATG GTG GGG AAC TGG GCT AAG GTC TTG ATT GTG ATG CTA 1452 Tyr Tyr Ser Met Val Gly Asn Trp Ala Lys Val Leu Ile Val Met Leu 365 370 375 CTC TTT GCC GGC GTC GAC GGG CAG ACC CGC GTG ACG GGG GCA CAA GTA 1500 Leu Phe Ala Gly Val Asp Gly Gln Thr Arg Val Thr Gly Ala Gln Val 380 385 390 GGC CGC ACC ACC AGT AGC CTT ACG TCC CTC TTC ACA CCA GGG CCG TCT 1548 Gly Arg Thr Thr Ser Ser Leu Thr Ser Leu Phe Thr Pro Gly Pro Ser 395 400 405 CAG AAC ATC CAG CTT GTA AAC TCT AAC GGC AGC TGG CAC ATC AAC AGA 1596 Gln Asn Ile Gln Leu Val Asn Ser Asn Gly Ser Trp His Ile Asn Arg 410 415 420 ACT GCC CTG AGT TGC AAC GAC TCC CTC AAA ACT GGG TTC CTT GCC GCG 1644 Thr Ala Leu Ser Cys Asn Asp Ser Leu Lys Thr Gly Phe Leu Ala Ala 425 430 435 440 CTG TTC TAC ACA CAT AAG TTC AAC GCG TCC GGA TGC CCG GAG CGC ATG 1692 Leu Phe Tyr Thr His Lys Phe Asn Ala Ser Gly Cys Pro Glu Arg Met 445 450 455 GCC AGC TGC CGC TCC ATT GAC ACG TTC GAC CAG GGG TGG GGT 1734 Ala Ser Cys Arg Ser Ile Asp Thr Phe Asp Gln Gly Trp Gly 460 465 470 配列番号：１０ 配列の長さ：１７３４ 配列の型：核酸 鎖の数：二本鎖 トポロジー：直鎖状 配列の種類：cDNA to genomic RNA ハイポセティカル：Ｎｏ アンチセンス：Ｎｏ 起源 生物名：Hepatitis C Virus 単離クローン名：JK4-A 組織の種類：肝臓細胞 GGCGACACTC CACCATAGAT CACTCCCCTG TGAGGAACTG CTGTCTTCAC GACGAAAGCG 60 TCTAGCCATG GCGTTAGTAT GACTGTCGTG CAGCCTGCAG GTCCCCCCCT CCCGGGAGAG 120 CCATAGTGGT CTGCGGAACC GGTGAGTACA CCGGAATTGC CAGGACGACC GGGTCCTTTC 180 TTGGATCACC CCGCTCAATG CCTGGAGATT TGGGCGTGCC CCCGCGAGAC TGCTAGCCGA 240 GTAGTGTTGG GTCGCGAAAG GCCTTGTGGT ACTGCCTGAT AGGGTGCTTG CGAGTGCCCC 300 GGGAGGTCTC GTAGACCGTG CACC ATG AGC ACA AAT CCT AAA CCT CAA 348 Met Ser Thr Asn Pro Lys Pro Gln 1 5 AGA AAA ACC AAA CGT AAC ACC AAC CGC CGC CCA CAG GAC GTT AAG TTC 396 Arg Lys Thr Lys Arg Asn Thr Asn Arg Arg Pro Gln Asp Val Lys Phe 10 15 20 CCG GGC GGT GGT CAG ATC GTT GGT GGA GTT TAC CTG TTG CCG CGC AGG 444 Pro Gly Gly Gly Gln Ile Val Gly Gly Val Tyr Leu Leu Pro Arg Arg 25 30 35 40 GGC CCC AGG TTG GGT GTG CGC GCG ACT AGG AAG ACT TCC GAG CGG TCG 492 Gly Pro Arg Leu Gly Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Ser 45 50 55 CAA CCT CGT GGA AGG CGA CAA CCT ATC CCC AAG GCT CGC CAG CCC GAG 540 Gln Pro Arg Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Gln Pro Glu 60 65 70 GGC AGG GCC TGT GCT CAG CCC GGG TAC CCT TGG CCC CTC TAT GGC AAT 588 Gly Arg Ala Cys Ala Gln Pro Gly Tyr Pro Trp Pro Leu Tyr Gly Asn 75 80 85 GAG GGT CTA GGG TGG GCA GGA TGG CTC CTG TCA CCC TAC GGC TCC CAG 636 Glu Gly Leu Gly Trp Ala Gly Trp Leu Leu Ser Pro Tyr Gly Ser Gln 90 95 100 CCT AGG TGG GGC CCC ACG GAC CCC CGG CGT AGG TCG CGT AAT TTG GGT 684 Pro Arg Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Asn Leu Gly 105 110 115 120 AAG GTC ATC GAT ACC CTC ACA TGC GGC TTC GCC GAC CTC ATG GGG TAC 732 Lys Val Ile Asp Thr Leu Thr Cys Gly Phe Ala Asp Leu Met Gly Tyr 125 130 135 ATT CCG CTC GTC GGC GCC CCC CTA GGA GGC GTT GCC AGG GCC CTG GCG 780 Ile Pro Leu Val Gly Ala Pro Leu Gly Gly Val Ala Arg Ala Leu Ala 140 145 150 CAT GGC GTC CGG GTT CTG GAG GAC GGC GTG AAC TAC GCA ACA GGG AAT 828 His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 155 160 165 CTG CCC GGT TGC TCT TTT TCT ATC TTC CTC TTG GCT TTG CTG TCC TGT 876 Leu Pro Gly Cys Ser Phe Ser Ile Phe Leu Leu Ala Leu Leu Ser Cys 170 175 180 CTG ACC GTC CCA GTT TCC ACC TAT GAA GTG CGC AAC GTG TCC GGG GTG 924 Leu Thr Val Pro Val Ser Thr Tyr Glu Val Arg Asn Val Ser Gly Val 185 190 195 200 TAC CAT GTC ACG AAC GAC TGC TCC AAC TCA AGT ATT GTG TAT GAG GCA 972 Tyr His Val Thr Asn Asp Cys Ser Asn Ser Ser Ile Val Tyr Glu Ala 205 210 215 GCG GAC ATG ATT ATG CAT ACC CCC GGG TGC GTA CCC TGC GTC CGG GAG 1020 Ala Asp Met Ile Met His Thr Pro Gly Cys Val Pro Cys Val Arg Glu 220 225 230 GGC AAT TCC TCC CGC TGC TGG GTA GCG CTC ACT CCC ACG CTC GCG GCC 1068 Gly Asn Ser Ser Arg Cys Trp Val Ala Leu Thr Pro Thr Leu Ala Ala 235 240 245 AGG AAC AGC AGC ATC CCC ACT ACG ACA ATA CGA CGT CAC GTC GAT TTG 1116 Arg Asn Ser Ser Ile Pro Thr Thr Thr Ile Arg Arg His Val Asp Leu 250 255 260 CTC GTT GGG GCA GCT GCT CTC TGC TCC GCT ATG TAC GTG GGG GAT CTC 1164 Leu Val Gly Ala Ala Ala Leu Cys Ser Ala Met Tyr Val Gly Asp Leu 265 270 275 280 TGT GGG TCT GTT TTC CTC GTC TCC CAA TTG TTC ACC TTC TCA CCT CGC 1212 Cys Gly Ser Val Phe Leu Val Ser Gln Leu Phe Thr Phe Ser Pro Arg 285 290 295 CGG TAT GAG ACG GTA CAG GAC TGC AAT TGC TCA CTC TAT CCC GGC CAT 1260 Arg Tyr Glu Thr Val Gln Asp Cys Asn Cys Ser Leu Tyr Pro Gly His 300 305 310 GTA TCA GGT CAT CGT ATG GCT TGG GAT ATG ATG ATG AAC TGG TCA CCT 1308 Val Ser Gly His Arg Met Ala Trp Asp Met Met Met Asn Trp Ser Pro 315 320 325 ACA ACA GCC TTA GTG GTG TCG CAG TTA CTC CGG ATC CCA CAA GCC GTC 1356 Thr Thr Ala Leu Val Val Ser Gln Leu Leu Arg Ile Pro Gln Ala Val 330 335 340 GTG GAC ATG GTG GTG GGG GCC CAC TGG GGA GTC CTG GCG GGC CTC GCC 1404 Val Asp Met Val Val Gly Ala His Trp Gly Val Leu Ala Gly Leu Ala 345 350 355 360 TAC TAT TCC ATG GTA GGG AAC TGG GCT AAG GTC TTG ATT GTG ATG CTA 1452 Tyr Tyr Ser Met Val Gly Asn Trp Ala Lys Val Leu Ile Val Met Leu 365 370 375 CTC TTT GCC GGC GTT GAC GGG ACT ACT TAC GTG TCA GTG GGG CAT GCA 1500 Leu Phe Ala Gly Val Asp Gly Thr Thr Tyr Val Ser Val Gly His Ala 380 385 390 AGC CGG ACC ACC CAG CGC GTT GCG TCC TTC TTC AGT CCC GGC TCG GCT 1548 Ser Arg Thr Thr Gln Arg Val Ala Ser Phe Phe Ser Pro Gly Ser Ala 395 400 405 CAG AAA ATC CAA CTT GTT AAC ACC AAC GGC AGT TGG CAC ATC AAC AGG 1596 Gln Lys Ile Gln Leu Val Asn Thr Asn Gly Ser Trp His Ile Asn Arg 410 415 420 ACT GCC CTG AAT TGC AAC GAG TCC ATC AAC ACT GGG TTC TTT GCC GCG 1644 Thr Ala Leu Asn Cys Asn Glu Ser Ile Asn Thr Gly Phe Phe Ala Ala 425 430 435 440 CTG TTC TAC GCA AAC AAG TTC AAC TCG TCT GGA TGC TCA GAG CGC ATG 1692 Leu Phe Tyr Ala Asn Lys Phe Asn Ser Ser Gly Cys Ser Glu Arg Met 445 450 455 GCT AGC TGT CGT CCC ATT GAC AAG TTC GCT CAA GGG TGG GGT 1734 Ala Ser Cys Arg Pro Ile Asp Lys Phe Ala Gln Gly Trp Gly 460 465 470 配列番号：１１ 配列の長さ：１７３４ 配列の型：核酸 鎖の数：二本鎖 トポロジー：直鎖状 配列の種類：cDNA to genomic RNA ハイポセティカル：Ｎｏ アンチセンス：Ｎｏ 起源 生物名：Hepatitis C Virus 単離クローン名：JK4-B 組織の種類：肝臓細胞 GGCGACACTC CACCATAGAT CACTCCCCTG TGAGGAACTG CTGTCTTCAC GACGAAAGCG 60 TCTAGCCATG GCGTTAGTAT GACTGTCGTG CAGCCTGCAG GTCCCCCCCT CCCGGGAGAG 120 CCATAGTGGT CTGCGGAACC GGTGAGTACA CCGGAATTGC CAGGACGACC GGGTCCTTTC 180 TTGGATCACC CCGCTCAATG CCTGGAGATT TGGGCGTGCC CCCGCGAGAC TGCTAGCCGA 240 GTAGTGTTGG GTCGCGAAAG GCCTTGTGGT ACTGCCTGAT AGGGTGCTTG CGAGTGCCCC 300 GGGAGGTCTC GTAGACCGTG CAAC ATG AGC ACA AAT CCT AAA CCT CAA 348 Met Ser Thr Asn Pro Lys Pro Gln 1 5 AGA AAA ACC AAA CGT AAC ACC AAC CGC CGC CCA CAG GAC GTC AAG TTT 396 Arg Lys Thr Lys Arg Asn Thr Asn Arg Arg Pro Gln Asp Val Lys Phe 10 15 20 CCG GGC GGT GGT CAG ATC GTT GGT GGA GTT TAC CTG TTG CCG CGC AGG 444 Pro Gly Gly Gly Gln Ile Val Gly Gly Val Tyr Leu Leu Pro Arg Arg 25 30 35 40 GGC CCC AGG TTG GGT GTG CGC GCG ACT AGG AAG ACT TCC GAG CGG TCG 492 Gly Pro Arg Leu Gly Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Ser 45 50 55 CAA CCT CGT GGA AGG CGA CAA CCT ATC CCC AAG GCT CGC CAG CCC GAG 540 Gln Pro Arg Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Gln Pro Glu 60 65 70 GGC AGG GCC TGT GCT CAG CCC GGG TAC CCT TGG CCC CTC TAT GGC AAT 588 Gly Arg Ala Cys Ala Gln Pro Gly Tyr Pro Trp Pro Leu Tyr Gly Asn 75 80 85 GAG GGT CTA GGG TGG GCA GGA TGG CTC CTG TCA CCC TAC GGC TCC CGG 636 Glu Gly Leu Gly Trp Ala Gly Trp Leu Leu Ser Pro Tyr Gly Ser Arg 90 95 100 CCT AGG TGG GGC CCC ACG GAC CCC CGG CGT AGG TCG CGT AAT TTG GGT 684 Pro Arg Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Asn Leu Gly 105 110 115 120 AAG GTC ATC GAT ACC CTC ACA TGC GGC TTC GCC GAC CTC ATG GGG TAC 732 Lys Val Ile Asp Thr Leu Thr Cys Gly Phe Ala Asp Leu Met Gly Tyr 125 130 135 ATT CCG CTC GTC GGC GCC CCC CTA GGA GGC GTT GCC AGG GCC CTG GCG 780 Ile Pro Leu Val Gly Ala Pro Leu Gly Gly Val Ala Arg Ala Leu Ala 140 145 150 CAT GGC GTC CGG GTT CTG GAG GAC GGC GTG AAC TAC GCA ACA GGG AAT 828 His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 155 160 165 TTG CCC GGT TGC TCT TTT TCT ATC TTC CTC TTG GCT TTG CTG TCC TGT 876 Leu Pro Gly Cys Ser Phe Ser Ile Phe Leu Leu Ala Leu Leu Ser Cys 170 175 180 CTG ACT GTC CCA GTT TCC ACT TAT GAA GTG CGC AAC GTG TCC GGG GTG 924 Leu Thr Val Pro Val Ser Thr Tyr Glu Val Arg Asn Val Ser Gly Val 185 190 195 200 TAC CAT GTC ACG AAC GAC TGC TCC AAC TCA AGT ATT GTG TAT GAA GCA 972 Tyr His Val Thr Asn Asp Cys Ser Asn Ser Ser Ile Val Tyr Glu Ala 205 210 215 GCG GAC ATG ATT ATG CAT ACC CCC GGG TGC GTA CCC TGC GTC CGG GAG 1020 Ala Asp Met Ile Met His Thr Pro Gly Cys Val Pro Cys Val Arg Glu 220 225 230 GGC AAT TCC TCC CGC TGC TGG GTA GCG CTC ACT CCC ACG CTC GCG GCC 1068 Gly Asn Ser Ser Arg Cys Trp Val Ala Leu Thr Pro Thr Leu Ala Ala 235 240 245 AGG AAC AGC AGC ATC CCC ACT ACG ACA ATA CGA CGA CAT GTC GAT TTG 1116 Arg Asn Ser Ser Ile Pro Thr Thr Thr Ile Arg Arg His Val Asp Leu 250 255 260 CTC GTT GGG GCA GCT GCT CTC TGC TCC GCT ATG TAC GTG GGG GAT CTC 1164 Leu Val Gly Ala Ala Ala Leu Cys Ser Ala Met Tyr Val Gly Asp Leu 265 270 275 280 TGT GGG TCT GTT TTC CTC GTC TCC CAA TTG TTC ACC TTC TCA CCT CGC 1212 Cys Gly Ser Val Phe Leu Val Ser Gln Leu Phe Thr Phe Ser Pro Arg 285 290 295 CGG TAT GAG ACG GTA CAG GAC TGC AAT TGC TCA CTC TAT CCC GGC CAC 1260 Arg Tyr Glu Thr Val Gln Asp Cys Asn Cys Ser Leu Tyr Pro Gly His 300 305 310 GTA TCA GGT CAT CGT ATG GCT TGG GAT ATG ATG ATG AAC TGG TCA CCT 1308 Val Ser Gly His Arg Met Ala Trp Asp Met Met Met Asn Trp Ser Pro 315 320 325 ACA ACA GCC TTG GTG GTG TCG CAG TTA CTC CGG ATC CCA CAA GCC GTC 1356 Thr Thr Ala Leu Val Val Ser Gln Leu Leu Arg Ile Pro Gln Ala Val 330 335 340 GTG GAC ATG GTG GTG GGG GCC CAC TGG GGA GTC CTG GCG GGC CTC GCC 1404 Val Asp Met Val Val Gly Ala His Trp Gly Val Leu Ala Gly Leu Ala 345 350 355 360 TAC TAT TCC ATG GTG GGA AAC TGG GCT AAG GTC TTG ATT GTG ATG CTA 1452 Tyr Tyr Ser Met Val Gly Asn Trp Ala Lys Val Leu Ile Val Met Leu 365 370 375 CTC TTT GCC GGC GTT GAC GGG ACT ACC ACC GTG TCA GGG GGG CAT GCA 1500 Leu Phe Ala Gly Val Asp Gly Thr Thr Thr Val Ser Gly Gly His Ala 380 385 390 AGC CAG ATC ACC CGG GGC GTT ACG TCC TTC TTC AGT CCT GGC TCG GCT 1548 Ser Gln Ile Thr Arg Gly Val Thr Ser Phe Phe Ser Pro Gly Ser Ala 395 400 405 CAG AAA ATC CAA CTT GTT AAC ACC AAC GGC AGT TGG CAC ATC AAC AGG 1596 Gln Lys Ile Gln Leu Val Asn Thr Asn Gly Ser Trp His Ile Asn Arg 410 415 420 ACT GCC CTG AAT TGC AAC GAG TCC ATC AAC ACT GGG TTC TTT GCC GCG 1644 Thr Ala Leu Asn Cys Asn Glu Ser Ile Asn Thr Gly Phe Phe Ala Ala 425 430 435 440 CTG TTT TAC GCA AAC AAG TTC AAC TCG TCT GGA TGC TCA GAG CGC ATG 1692 Leu Phe Tyr Ala Asn Lys Phe Asn Ser Ser Gly Cys Ser Glu Arg Met 445 450 455 GCT AGC TGT CGT CCC ATT GAC AAA TTC GCC CAA GGG TGG GGT 1734 Ala Ser Cys Arg Pro Ile Asp Lys Phe Ala Gln Gly Trp Gly 460 465 470 配列番号：１２ 配列の長さ：１７３４ 配列の型：核酸 鎖の数：二本鎖 トポロジー：直鎖状 配列の種類：cDNA to genomic RNA ハイポセティカル：Ｎｏ アンチセンス：Ｎｏ 起源 生物名：Hepatitis C Virus 単離クローン名：JK4-C 組織の種類：肝臓細胞 GGCGACACTC CACCATAGAT CACTCCCCTG TGAGGAACTG CTGTCTTCAC GACGAAAGCG 60 TCTAGCCATG GCGTTAGTAT GACTGTCGTG CAGCCTGCAG GTCCCCCCCT CCCGGGAGAG 120 CCATAGTGGT CTGCGGAACC GGTGAGTACA CCGGAATTGC CAGGACGACC GGGTCCTTTC 180 TTGGATCACC CCGCTCAATG CCTGGAGATT TGGGCGTGCC CCCGCGAGAC TGCTAGCCGA 240 GTAGTGTTGG GTCGCGAAAG GCCTTGTGGT ACTGCCTAAT AGGGTGCTTG CGAGTGCCCC 300 GGGAGGTCTC GTAGACCGTG CACC ATG AGC ACA AAT CCT AAA CCT CAA 348 Met Ser Thr Asn Pro Lys Pro Gln 1 5 AGA AAA ACC AAA CGT AAC ACC AAC CGC CGC CCA CAG GAC GTC AAG TTC 396 Arg Lys Thr Lys Arg Asn Thr Asn Arg Arg Pro Gln Asp Val Lys Phe 10 15 20 CCG GGC GGT GGT CAG ATC GTT GGT GGA GTT TAC CTG TTG CCG CGC AGG 444 Pro Gly Gly Gly Gln Ile Val Gly Gly Val Tyr Leu Leu Pro Arg Arg 25 30 35 40 GGC CCC AGG TTG GGT GTG CGC GCG ACT AGG AAG ACT TCC GAG CGG TCG 492 Gly Pro Arg Leu Gly Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Ser 45 50 55 CAA CCT CGT GGA AGG CGA CAA CCT ATC CCC AAG GCT CGC CAG CCC GAG 540 Gln Pro Arg Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Gln Pro Glu 60 65 70 GGC AGG GCC TGT GCT CAG CCC GGG TAC CCT TGG CCC CTC TAT GGC AAT 588 Gly Arg Ala Cys Ala Gln Pro Gly Tyr Pro Trp Pro Leu Tyr Gly Asn 75 80 85 GAG GGT CTA GGG TGG GCA GGA TGG CTC CTG TCA CCC TAC GGC TCC CGG 636 Glu Gly Leu Gly Trp Ala Gly Trp Leu Leu Ser Pro Tyr Gly Ser Arg 90 95 100 CCT AGG TGG GGC CCC ACG GAC CCC CGG CGT AGG TCG CGT AAT TTG GGT 684 Pro Arg Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Asn Leu Gly 105 110 115 120 AAG GTC ATC GAT ACC CTC ACA TGC GGC TTC GCC GAC CTC ATG GGG TAC 732 Lys Val Ile Asp Thr Leu Thr Cys Gly Phe Ala Asp Leu Met Gly Tyr 125 130 135 ATT CCG CTC GTC GGC GCC CCC CTA GGA GGC GTT GCC AGG GCC CTG GCG 780 Ile Pro Leu Val Gly Ala Pro Leu Gly Gly Val Ala Arg Ala Leu Ala 140 145 150 CAT GGC GTC CGG GTT CTG GAG GAC GGC GTG AAC TAC GCA ACA GGG AAT 828 His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 155 160 165 CTG CCC GGT TGC TCT TTT TCT ATC TTC CTC TTG GCT TTG CTG TCC TGT 876 Leu Pro Gly Cys Ser Phe Ser Ile Phe Leu Leu Ala Leu Leu Ser Cys 170 175 180 CTG ACC GTC CCA GTT TCC ACT TAT GAA GTG CGC AAC GTG TCC GGG GTG 924 Leu Thr Val Pro Val Ser Thr Tyr Glu Val Arg Asn Val Ser Gly Val 185 190 195 200 TAC CAT GTC ACG AAC GAC TGC TCC AAC TCA AGT ATT GTG TAT GAG GCA 972 Tyr His Val Thr Asn Asp Cys Ser Asn Ser Ser Ile Val Tyr Glu Ala 205 210 215 GCG GAC ATG ATT ATG CAT ACC CCC GGG TGC GTA CCC TGC GTC CGG GAG 1020 Ala Asp Met Ile Met His Thr Pro Gly Cys Val Pro Cys Val Arg Glu 220 225 230 GGC AAT TCC TCC CGC TGC TGG GTA GCG CTC ACT CCC ACG CTC GCG GCC 1068 Gly Asn Ser Ser Arg Cys Trp Val Ala Leu Thr Pro Thr Leu Ala Ala 235 240 245 AGG AAC AGC AGC ATC CCC ACT ACG ACA ATA CGA CGA CAT GTC GAT TTG 1116 Arg Asn Ser Ser Ile Pro Thr Thr Thr Ile Arg Arg His Val Asp Leu 250 255 260 CTC GTT GGG GCA GCT GCT CTC TGC TCC GCT ATG TAC GTG GGG GAT CTC 1164 Leu Val Gly Ala Ala Ala Leu Cys Ser Ala Met Tyr Val Gly Asp Leu 265 270 275 280 TGT GGG TCT GTT TTT CTC GTC TCC CAA TTG TTC ACC TTC TCA CCT CGC 1212 Cys Gly Ser Val Phe Leu Val Ser Gln Leu Phe Thr Phe Ser Pro Arg 285 290 295 CGG TAT GAG ACG GTA CAG GAC TGC AAT TGC TCA CTC TAT CCC GGC CAC 1260 Arg Tyr Glu Thr Val Gln Asp Cys Asn Cys Ser Leu Tyr Pro Gly His 300 305 310 GTA TCA GGT CAT CGT ATG GCT TGG GAT ATG ATG ATG AAC TGG TCA CCT 1308 Val Ser Gly His Arg Met Ala Trp Asp Met Met Met Asn Trp Ser Pro 315 320 325 ACA ACA GCC TTA GTG GTG TCG CAG TTA CTC CGG ATC CCA CAA GCC GTC 1356 Thr Thr Ala Leu Val Val Ser Gln Leu Leu Arg Ile Pro Gln Ala Val 330 335 340 GTG GAC ATG GTG GTG GGG GCC CAC TGG GGA GTC CTG GCG GGC CTC GCC 1404 Val Asp Met Val Val Gly Ala His Trp Gly Val Leu Ala Gly Leu Ala 345 350 355 360 TAC TAT TCC ATG GTG GGG AAC TGG GCT AAG GTC TTG ATT GTG ATG CTA 1452 Tyr Tyr Ser Met Val Gly Asn Trp Ala Lys Val Leu Ile Val Met Leu 365 370 375 CTC TTT GCC GGC GTT GAC GGG ACT ACC TAC GTG TCA GTG GGG CAT GCA 1500 Leu Phe Ala Gly Val Asp Gly Thr Thr Tyr Val Ser Gly Gly His Ala 380 385 390 AGC CGG ACC ACC CAG CGC GTT GCG TCC TTC TTC AGT CCC GGC TCG GCT 1548 Ser Arg Thr Thr Gln Arg Val Ala Ser Phe Phe Ser Pro Gly Ser Ala 395 400 405 CAG AAA ATC CAA CTT GTT AAC ACC AAC GGC AGT TGG CAC ATC AAC AGG 1596 Gln Lys Ile Gln Leu Val Asn Thr Asn Gly Ser Trp His Ile Asn Arg 410 415 420 ACT GCC CTG AAT TGC AAC GAG TCC ATC AAC ACT GGG TTC TTT GCC GCG 1644 Thr Ala Leu Asn Cys Asn Glu Ser Ile Asn Thr Gly Phe Phe Ala Ala 425 430 435 440 CTG TTC TAC GCA AAC AAG TTC AAC TCG TCT GGA TGC TCA GAG CGC ATG 1692 Leu Phe Tyr Ala Asn Lys Phe Asn Ser Ser Gly Cys Ser Glu Arg Met 445 450 455 GCT AGC TGT CGT CCC ATT GAC AGG TTC GCC CAA GGG TGG GGT 1734 Ala Ser Cys Arg Pro Ile Asp Arg Phe Ala Gln Gly Trp Gly 460 465 470 配列番号：１３ 配列の長さ：１７３４ 配列の型：核酸 鎖の数：二本鎖 トポロジー：直鎖状 配列の種類：cDNA to genomic RNA ハイポセティカル：Ｎｏ アンチセンス：Ｎｏ 起源 生物名：Hepatitis C Virus 単離クローン名：JK5-A 組織の種類：肝臓細胞 GGCGACACTC CACCATAGAT CACTCCCCTG TGAGGAACTG CTGTCTTCAC GACGAAAGCG 60 TCTAGCCATG GCGTTAGTAT GACTGTCGTA CAGCCTGCAG GCCCCCCCCT CCCGGGAGAG 120 CCATAGTGGT CTGCGGAACC GGTGAGTACA CCGGAATTGC CAGGACGACC GGGTCCTTTC 180 TTGGATAACC CTACTCTATG CCCGGCCATT TGGGCGTGCC CCCGCAAGAC TGCTAGCCGA 240 GTAGCGTTGG GTTGCGAAAG GCCTTGTGGT ACTGCCTGAT AGGGTGCTTG CGAGTGCCCC 300 GGGAGGTCTC GTAGACCGTG CACC ATG AGC ACA AAT CCC AAA CCT CAA 348 Met Ser Thr Asn Pro Lys Pro Gln 1 5 AGA AAA ACC AAA CGT AAC ACC AAC CGC CGC CCA CAG GAC GTT AAG TTT 396 Arg Lys Thr Lys Arg Asn Thr Asn Arg Arg Pro Gln Asp Val Lys Phe 10 15 20 CCG GGC GGC GGC CAG ATC GTT GGC GGA GTA TAC TTG TTG CCG CGC AGG 444 Pro Gly Gly Gly Gln Ile Val Gly Gly Val Tyr Leu Leu Pro Arg Arg 25 30 35 40 GGC CCC AGG TTG GGT GTG CGC GCG ACT AGG AAG ACT TCC GAG CGG TCG 492 Gly Pro Arg Leu Gly Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Ser 45 50 55 CAA CCT CGT GGA AGG CGA CAA CCT ATC CCC AAG GCT CGC CAG CCC GAG 540 Gln Pro Arg Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Gln Pro Glu 60 65 70 GGC AGG GCC TGG GCT CAG CCC GGG TAC CCT TGG CCC CTC TAT GGC AAT 588 Gly Arg Ala Trp Ala Gln Pro Gly Tyr Pro Trp Pro Leu Tyr Gly Asn 75 80 85 GAG GGT CTG GGG TGG GCA GGA TGG CTC CTG TCA CCC CGT GGC TCC CGG 636 Glu Gly Leu Gly Trp Ala Gly Trp Leu Leu Ser Pro Arg Gly Ser Arg 90 95 100 CCT AGT TGG GGC CCC ACG GAC CCC CGG CGT AGG TCG CGT AAT TTG GGT 684 Pro Ser Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Asn Leu Gly 105 110 115 120 AAG GTC ATC GAT ACC CTC ACA TGC GGC TTC GCC GAC CTC ATG GGG TAC 732 Lys Val Ile Asp Thr Leu Thr Cys Gly Phe Ala Asp Leu Met Gly Tyr 125 130 135 ATT CCG CTC GTC GGC GCC CCC CTA GGA GGC GCT GCC AGG GCC CTG GCG 780 Ile Pro Leu Val Gly Ala Pro Leu Gly Gly Ala Ala Arg Ala Leu Ala 140 145 150 CAT GGC GTC CGG GTT CTG GAG GAC GGC GTG AAC TAC GCA ACA GGG AAC 828 His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 155 160 165 CTG CCC GGT TGC TCT TTC TCT ATC TTC CTC TTG GCT CTG CTG TCT TGT 876 Leu Pro Gly Cys Ser Phe Ser Ile Phe Leu Leu Ala Leu Leu Ser Cys 170 175 180 TTG ACC ATC CCA GCA TCC GCT TAT GAA GTG CGC AAC GTG TCC GGG TTG 924 Leu Thr Ile Pro Ala Ser Ala Tyr Glu Val Arg Asn Val Ser Gly Leu 185 190 195 200 TAC CAT GTC ACG AAC GAC TGC TCC AAC TCA AGT ATT GTG TAT GAG GCA 972 Tyr His Val Thr Asn Asp Cys Ser Asn Ser Ser Ile Val Tyr Glu Ala 205 210 215 GCG GAC TTG ATC ATG CAC ACC CCT GGG TGC GTG CCC TGC GTC CTG GAG 1020 Ala Asp Leu Ile Met His Thr Pro Gly Cys Val Pro Cys Val Leu Glu 220 225 230 GGC AAC TCC TCC CGC TGC TGG GTA GCG CTC ACT CCC ACG CTT GCG GCC 1068 Gly Asn Ser Ser Arg Cys Trp Val Ala Leu Thr Pro Thr Leu Ala Ala 235 240 245 AGG AAC AAC AGC ATC CCC ACT ACG ACA ATA CGA CGT CAC GTC GAT TTG 1116 Arg Asn Asn Ser Ile Pro Thr Thr Thr Ile Arg Arg His Val Asp Leu 250 255 260 CTC GTT GGG GCG GCT GCT TTC TGC TCC GCT ATG TAC GTG GGG GAC CTC 1164 Leu Val Gly Ala Ala Ala Phe Cys Ser Ala Met Tyr Val Gly Asp Leu 265 270 275 280 TGC GGA TCT GTT TTC CTC GTC TCC CAG CTA TTC ACC TTC TCA CCT CGC 1212 Cys Gly Ser Val Phe Leu Val Ser Gln Leu Phe Thr Phe Ser Pro Arg 285 290 295 CGG TAT GAG ACG GTA CAA GAC TGC AAC TGC TCA CTC TAT CCC GGC CAC 1260 Arg Tyr Glu Thr Val Gln Asp Cys Asn Cys Ser Leu Tyr Pro Gly His 300 305 310 GTA TCA GGT CAC CGC ATG GCT TGG GAT ATG ATG ATG AAC TGG TCG CCC 1308 Val Ser Gly His Arg Met Ala Trp Asp Met Met Met Asn Trp Ser Pro 315 320 325 ACA ACA GCC CTA GTG GTA TCG CAG TTA CTC CGG ATC CCA CAA GCC GTC 1356 Thr Thr Ala Leu Val Val Ser Gln Leu Leu Arg Ile Pro Gln Ala Val 330 335 340 GTG GAC ATG GTG GCG GGG GCC CAC TGG GGA GTC TTG GCG GGC CTC GCC 1404 Val Asp Met Val Ala Gly Ala His Trp Gly Val Leu Ala Gly Leu Ala 345 350 355 360 TAC TAT TCC ATG GTG GGG AAC TGG GCT AAG GTC TTG ATT GTG ATG CTA 1452 Tyr Tyr Ser Met Val Gly Asn Trp Ala Lys Val Leu Ile Val Met Leu 365 370 375 CGC TTT GCC GGC GTC AAC GGG CAG ACC CGC GTG ACG GGG GCA CAA GTA 1500 Arg Phe Ala Gly Val Asn Gly Gln Thr Arg Val Thr Gly Ala Gln Val 380 385 390 GGC CGC ACC ACC AGT AGC CTT ACG TCC CTC TTC ACA CCA GGG CCG TCT 1548 Gly Arg Thr Thr Ser Ser Leu Thr Ser Leu Phe Thr Pro Gly Pro Ser 395 400 405 CAG AAC ATC CAG CTT GTA AAC TCT AAC GGC AGC TGG CAC ATT AAC AGA 1596 Gln Asn Ile Gln Leu Val Asn Ser Asn Gly Ser Trp His Ile Asn Arg 410 415 420 ACT GCC CTG AGT TGC AAC GAC TCC CTC AAA ACT GGG TTC CTT GCC GCG 1644 Thr Ala Leu Ser Cys Asn Asp Ser Leu Lys Thr Gly Phe Leu Ala Ala 425 430 435 440 CTG TTC TAC ACA CAT AAG TTC AAC GCG TCC GGA TGC CCG GAG CGC ATG 1692 Leu Phe Tyr Thr His Lys Phe Asn Ala Ser Gly Cys Pro Glu Arg Met 445 450 455 GCC AGC TGC CGC TCC ATT GAC ACG TTC GAC CAG GGG TGG GGT 1734 Ala Ser Cys Arg Ser Ile Asp Thr Phe Asp Gln Gly Trp Gly 460 465 470 配列番号：１４ 配列の長さ：１７３４ 配列の型：核酸 鎖の数：二本鎖 トポロジー：直鎖状 配列の種類：cDNA to genomic RNA ハイポセティカル：Ｎｏ アンチセンス：Ｎｏ 起源 生物名：Hepatitis C Virus 単離クローン名：JK5-B 組織の種類：肝臓細胞 GGCGACACTC CACCATAGAT CACTCCCCTG TGAGGAACTG CTGTCTTCAC GACGAAAGCG 60 TCTAGCCATG GCGTTAGTAT GACTGTCGTA CAGCCTGCAG GCCCCCCCCT CCCGGGAGAG 120 CCATAGTGGT CTGCGGAACC GGTGAGTACA CCGGAATTGC CAGGACGACC GGGTCCTTTC 180 TTGGATAACC CTGCTCTATG CCCGGCCATT TGGGCGTGCC CCCGCAAGAC TGCTAGCCGA 240 GTAGCGTTGG GTTGCGAAAG GCCTTGTGGT ACTGCCTGAT AGGGTGCTTG CGAGTGCCCC 300 GGGAGGTCTC GTAGACCGTG CACC ATG AGC ACA AAT CCC AAA CCT CAA 348 Met Ser Thr Asn Pro Lys Pro Gln 1 5 AGA AAA ACC AAA CGT AAC ACC AAC CGC CGC CCA CAG GAC GTC AAG TTC 396 Arg Lys Thr Lys Arg Asn Thr Asn Arg Arg Pro Gln Asp Val Lys Phe 10 15 20 CCG GGT GGT GGT CAG ATC GTT GGT GGA GTT TAC CTG TTT CCG CGC AGG 444 Pro Gly Gly Gly Gln Ile Val Gly Gly Val Tyr Leu Phe Pro Arg Arg 25 30 35 40 GGC CCC AGG TTG GGT GTG CGC GCG ACA AGG AAG ACT TCC GAG CGG TCG 492 Gly Pro Arg Leu Gly Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Ser 45 50 55 CAA CCT CGT GGA AGG CGA CAA CCT ATC CCC AAG GCT CGC CAG CCC GAG 540 Gln Pro Arg Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Gln Pro Glu 60 65 70 GGC AGG GCC TGG GCT CAG CCC GGG TAC CCT TGG CCC CTC TAT GGC AAT 588 Gly Arg Ala Trp Ala Gln Pro Gly Tyr Pro Trp Pro Leu Tyr Gly Asn 75 80 85 GAG GGT CTG GGG TGG GCA GGA TGG CTC CTG TCA CCC CGT GGC TCC CGG 636 Glu Gly Leu Gly Trp Ala Gly Trp Leu Leu Ser Pro Arg Gly Ser Arg 90 95 100 CCT AGT TGG GGC CCC ACG GAC CCC CGG CGT AGG TCG CGT AAT TTG GGT 684 Pro Ser Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Asn Leu Gly 105 110 115 120 AAG GTC ATC GAT ACC CTC ACA TGC GGC TTC GCC GAC CTC ATG GGG TAC 732 Lys Val Ile Asp Thr Leu Thr Cys Gly Phe Ala Asp Leu Met Gly Tyr 125 130 135 ATT CCG CTC GTC GGC GCC CCC CTA GGA GGC GCT GCC AGG GCC CTG GCG 780 Ile Pro Leu Val Gly Ala Pro Leu Gly Gly Ala Ala Arg Ala Leu Ala 140 145 150 CAT GGC GTC CGG GTT CTG GAG GAC GGC GTG AAC TAC GCA ACA GGG AAC 828 His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 155 160 165 CTG CCC GGT TGC TCT TTC TCT ATC TTC CTC TTG GCT CTG CTG TCT TGT 876 Leu Pro Gly Cys Ser Phe Ser Ile Phe Leu Leu Ala Leu Leu Ser Cys 170 175 180 TTG ACC ATC CCA GCA TCC GCT TAT GAA GTG CGC AAC GTG TCC GGG TTG 924 Leu Thr Ile Pro Ala Ser Ala Tyr Glu Val Arg Asn Val Ser Gly Leu 185 190 195 200 TAC CAT GTC ACG AAC GAC TGC TCC AAC TCA AGT ATT GTG TAT GAG GCA 972 Tyr His Val Thr Asn Asp Cys Ser Asn Ser Ser Ile Val Tyr Glu Ala 205 210 215 GCG GAC TTG ATC ATG CAC ACC CCT GGG TGC GTG CCC TGC GTC CTG GAG 1020 Ala Asp Leu Ile Met His Thr Pro Gly Cys Val Pro Cys Val Leu Glu 220 225 230 GGC AAC TCC TCC CGC TGC TGG GTA GCG CTC ACT CCC ACG CTT GCG GCC 1068 Gly Asn Ser Ser Arg Cys Trp Val Ala Leu Thr Pro Thr Leu Ala Ala 235 240 245 AGG AAC AAC AGC ATC CCC ACT ACG ACA ATA CGA CGT CAC GTC GAT TTG 1116 Arg Asn Asn Ser Ile Pro Thr Thr Thr Ile Arg Arg His Val Asp Leu 250 255 260 CTC GTT GGG GCG GCT GCT TTC TGC TCC GCT ATG TAC GTG GGG GAC CTC 1164 Leu Val Gly Ala Ala Ala Phe Cys Ser Ala Met Tyr Val Gly Asp Leu 265 270 275 280 TGC GGA TCT GTT TTC CTC GTC TCC CAG CTA TTC ACC TTC TCA CCT CGC 1212 Cys Gly Ser Val Phe Leu Val Ser Gln Leu Phe Thr Phe Ser Pro Arg 285 290 295 CGG TAT GAG ACG GTA CAA GAC TGC AAC TGC TCA CTC TAT CCC GGC CAC 1260 Arg Tyr Glu Thr Val Gln Asp Cys Asn Cys Ser Leu Tyr Pro Gly His 300 305 310 GTA TCA GGT CAC CGC ATG GCT TGG GAT ATG ATG ATG AAC TGG TCG CCC 1308 Val Ser Gly His Arg Met Ala Trp Asp Met Met Met Asn Trp Ser Pro 315 320 325 ACA ACA GCC CTA GTG GTA TCG CAG TTA CTC CGG ATC CCA CAA GCC GTC 1356 Thr Thr Ala Leu Val Val Ser Gln Leu Leu Arg Ile Pro Gln Ala Val 330 335 340 GTG GAC ATG GTG GCG GGG GCC CAC TGG GGA GTC TTG GCG GGC CTC GCC 1404 Val Asp Met Val Ala Gly Ala His Trp Gly Val Leu Ala Gly Leu Ala 345 350 355 360 TAC TAT TCC ATG GTG GGG AAC TGG GCT AAG GTC TTG ATT GTG ATG CTA 1452 Tyr Tyr Ser Met Val Gly Asn Trp Ala Lys Val Leu Ile Val Met Leu 365 370 375 CGC TTT GCC GGC GTC GAC GGG CAG ACC CGC GTG ACG GGG GCA CAA GTA 1500 Arg Phe Ala Gly Val Asp Gly Gln Thr Arg Val Thr Gly Ala Gln Val 380 385 390 GGC CGC ACC ACC AGT AGC CTT ACG TCC CTC TTC ACA CCA GGG CCG TCT 1548 Gly Arg Thr Thr Ser Ser Leu Thr Ser Leu Phe Thr Pro Gly Pro Ser 395 400 405 CAG AAC ATC CAG CTT GTA AAC TCT AAC GGC AGC TGG CAC ATC AAC AGA 1596 Gln Asn Ile Gln Leu Val Asn Ser Asn Gly Ser Trp His Ile Asn Arg 410 415 420 ACT GCC CTG AGT TGC AAC GAC TCC CTC AAA ACT GGG TTC CTT GCC GCG 1644 Thr Ala Leu Ser Cys Asn Asp Ser Leu Lys Thr Gly Phe Leu Ala Ala 425 430 435 440 CTG TTC TAC ACA CAT AAG TTC AAC GCG TCC GGA TGC CCG GAG CGC ATG 1692 Leu Phe Tyr Thr His Lys Phe Asn Ala Ser Gly Cys Pro Glu Arg Met 445 450 455 GCC AGC TGC CGC TCC ATT GAC ACG TTC GAC CAG GGG TGG GGT 1734 Ala Ser Cys Arg Ser Ile Asp Thr Phe Asp Gln Gly Trp Gly 460 465 470 配列番号：１５ 配列の長さ：１７３４ 配列の型：核酸 鎖の数：二本鎖 トポロジー：直鎖状 配列の種類：cDNA to genomic RNA ハイポセティカル：Ｎｏ アンチセンス：Ｎｏ 起源 生物名：Hepatitis C Virus 単離クローン名：JK5-C 組織の種類：肝臓細胞 GGCGACACTC CACCATAGAT CACTCCCCTG TGAGGAACTG CTGTCTTCAC GACGAAAGCG 60 TCTAGCCATG GCGTTAGTAT GACTGTCGTA CAGCCTGCAG GCCCCCCCCT CCCGGGAGAG 120 CCATAGTGGT CTGCGGAACC GGTGAGTACA CCGGAATTGC CAGGACGACC GGGTCCTTTC 180 TTGGATAACC CTGCTCTATG CCCGGCCATT TGGGCGTGCC CCCGCAAGAC TGCTAGCCGA 240 GTAGCGTTGG GTTGCGAAAG GCCTTGTGGT ACTGCCTAAT AGGGTGCTTG CGAGTGCCCC 300 GGGAGGTCTC GTAGACCGTG CACC ATG AGC ACA AAT CCC AAA CCT CAA 348 Met Ser Thr Asn Pro Lys Pro Gln 1 5 AGA CAA ACC AAA CGT AAC ACC AAC CGC CGC CCA CAG GAC GTT AAG TTT 396 Arg Gln Thr Lys Arg Asn Thr Asn Arg Arg Pro Gln Asp Val Lys Phe 10 15 20 CCG GGC GGC GGC CAG ATC GTT GGC GGA GTA TAC TTG TTG CCG CGC AGG 444 Pro Gly Gly Gly Gln Ile Val Gly Gly Val Tyr Leu Leu Pro Arg Arg 25 30 35 40 GGC CCC AGG TTG GGT GTG CGC GCG ACG AGG AAG ACT TCG GAG CGG TCC 492 Gly Pro Arg Leu Gly Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Ser 45 50 55 CAG CCA CGT GGG AGG CGC CAG CCC ATC CCC AAA GAT CGG CGC TCC ACT 540 Gln Pro Arg Gly Arg Arg Gln Pro Ile Pro Lys Asp Arg Arg Ser Thr 60 65 70 GGC AAG TCC TGG GAA GAG CCA GGA TAC CCT TGG CCC CTA TAT GGG AAT 588 Gly Lys Ser Trp Glu Glu Pro Gly Tyr Pro Trp Pro Leu Tyr Gly Asn 75 80 85 GAG GGA CTC GGC TGG GCA GGA TGG CTC CTG TCT CCC CGA GGT TCC CGT 636 Glu Gly Leu Gly Trp Ala Gly Trp Leu Leu Ser Pro Arg Gly Ser Arg 90 95 100 CCC TCT TGG GGC CCC AAT GAC CCC CGG CAT AGG TCG CGC AAC GTG GGT 684 Pro Ser Trp Gly Pro Asn Asp Pro Arg His Arg Ser Arg Asn Val Gly 105 110 115 120 AAG GTC ATC GGT ACC CTC ATA TGC GGC CTC GCC GAC CTC ATG GGG TAC 732 Lys Val Ile Gly Thr Leu Ile Cys Gly Leu Ala Asp Leu Met Gly Tyr 125 130 135 ATT CCG CTC GTC AGC GCC CCC CTA GGA GGC GCT GCC AGG GCC CTG GCG 780 Ile Pro Leu Val Ser Ala Pro Leu Gly Gly Ala Ala Arg Ala Leu Ala 140 145 150 CAT GGC GTC CGG GTT CTG GAG GAC GGC GTG AAC TAC GCA ACA GGG AAC 828 His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 155 160 165 CTG CCC GGT TGC TCT TTC TCT ATC TTC CTC TTG GCT CTG CTG TCT TGT 876 Leu Pro Gly Cys Ser Phe Ser Ile Phe Leu Leu Ala Leu Leu Ser Cys 170 175 180 TTG ACC ATC CCA GCA TCC GCT TAT GAA GTG CGC AAC GTG TCC GGG TTG 924 Leu Thr Ile Pro Ala Ser Ala Tyr Glu Val Arg Asn Val Ser Gly Leu 185 190 195 200 TAC CAT GTC ACG AAC GAC TGC TCC AAC TCA AGT ATT GTG TAT CAG GCA 972 Tyr His Val Thr Asn Asp Cys Ser Asn Ser Ser Ile Val Tyr Gln Ala 205 210 215 GCG GAC TTG GTC ATG CAC ACC CCT GGG TGC GTG CCC TGC GTC CTG GAG 1020 Ala Asp Leu Val Met His Thr Pro Gly Cys Val Pro Cys Val Leu Glu 220 225 230 GGC AAC TCC TCC CGC TGC TGG GTA GCG CTC ACT CCC ACG CTT GCG GCC 1068 Gly Asn Ser Ser Arg Cys Trp Val Ala Leu Thr Pro Thr Leu Ala Ala 235 240 245 AGG AAC AAC AGC ATC CCC ACT ACG ACA ATA CGA CGT CAC GTC GAT TTG 1116 Arg Asn Asn Ser Ile Pro Thr Thr Thr Ile Arg Arg His Val Asp Leu 250 255 260 CTC GTT GGG GCG GCT GCT TTC TGC TCC GCT ATG TAC GTG GGG GAC CTC 1164 Leu Val Gly Ala Ala Ala Phe Cys Ser Ala Met Tyr Val Gly Asp Leu 265 270 275 280 TGC GGC GGG GTG ATG CTC GCC TCC CAG CTA TTC GTC GTC TCG CCG CAA 1212 Cys Gly Gly Val Met Leu Ala Ser Gln Leu Phe Val Val Ser Pro Gln 285 290 295 CAT CAT TGG TTT GTG CAG GAA TGC AAT TGC TCC ATC TAC CCT GGT ACC 1260 His His Trp Phe Val Gln Glu Cys Asn Cys Ser Ile Tyr Pro Gly Thr 300 305 310 ATC ACT GGG CAC CGC ATG GCT TGG GAT ATG ATG ATG AAC TGG TCG CCC 1308 Ile Thr Gly His Arg Met Ala Trp Asp Met Met Met Asn Trp Ser Pro 315 320 325 ACA ACA GCC CTA GTG GTA TCG CAG TTA CTC CGG ATC CCA CAA GCC GTC 1356 Thr Thr Ala Leu Val Val Ser Gln Leu Leu Arg Ile Pro Gln Ala Val 330 335 340 GTG GAC ATG GTG GCG GGG GCC CAC TGG GGA GTC TTG GCG GGC CTC GCC 1404 Val Asp Met Val Ala Gly Ala His Trp Gly Val Leu Ala Gly Leu Ala 345 350 355 360 TAC TAT TCC ATG GTG GGG AAC TGG GCT AAG GTC TTG ATT GTG ATG CTA 1452 Tyr Tyr Ser Met Val Gly Asn Trp Ala Lys Val Leu Ile Val Met Leu 365 370 375 CGC TTT GCC GGC GTC GAC GGG CAG ACC CGC GTG ACG GGG GCA CAA GTA 1500 Arg Phe Ala Gly Val Asp Gly Gln Thr Arg Val Thr Gly Ala Gln Val 380 385 390 GGC CGC ACC ACC AGT AGC CTT ACG TCC CTC TTC ACA CCA GGG CCG TCT 1548 Gly Arg Thr Thr Ser Ser Leu Thr Ser Leu Phe Thr Pro Gly Pro Ser 395 400 405 CAG AAC ATC CAG CTT GTA AAC TCT AAC GGC AGC TGG CAC ATC AAC AGA 1596 Gln Asn Ile Gln Leu Val Asn Ser Asn Gly Ser Trp His Ile Asn Arg 410 415 420 ACT GCC CTG AGT TGC AAC GAC TCC CTC AAA ACT GGG TTC CTT GCC GCG 1644 Thr Ala Leu Ser Cys Asn Asp Ser Leu Lys Thr Gly Phe Leu Ala Ala 425 430 435 440 CTG TTC TAC ACA CAT AAG TTC AAC GCG TCT GGA TGC CCG GAG CGC ATG 1692 Leu Phe Tyr Thr His Lys Phe Asn Ala Ser Gly Cys Pro Glu Arg Met 445 450 455 GCC AGC TGC CGC TCC ATT GAC ACG TTC GAC CAG GGG TGG GGT 1734 Ala Ser Cys Arg Ser Ile Asp Thr Phe Asp Gln Gly Trp Gly 460 465 470

【図面の簡単な説明】

【図１】NANB 型肝炎であると確定診断された患者 3 名
と健常人 1 名の血清を被験資料とし、本発明による DN
A クローンの一部領域をプライマーとして PCR を実施
した場合における PCR産物の電気泳動パターンを描記し
た図であり、レーン 2- 5 はプライマーセット A を、
レーン 7 - 10 はプライマーセットB を用いた場合のも
のであって、レーン 2 - 4 及び 7 -9 は NANB 型肝炎
患者血清を、レーン 5 及び 10 は健常人血清を用いた
場合であり、レーン 1 及び 6 はサイズマーカーを示し
ている。

───────────────────────────────────────────────────── フロントページの続き (72)発明者 澤井 喜一 愛知県名古屋市東区東外堀町35番地 株式 会社三和化学研究所内

Claims (10)

【特許請求の範囲】
1. 【請求項１】 ヒト C 型肝炎ウイルス遺伝子をコード
   する約 9500 個のヌクレオチドを含有する一本鎖 DNA
   又は該一本鎖 DNA とその相補一本鎖 DNA とからなる二
   本鎖 DNA。
2. 【請求項２】 ヒト C 型肝炎ウイルス遺伝子の 5'-側
   翻訳不能領域と、それに続くアミノ酸配列の一部をコー
   ドする約 1800 個のヌクレオチドを含有する一本鎖 DNA
   又は該一本鎖 DNA とその相補一本鎖 DNA とからなる
   二本鎖 DNA。
3. 【請求項３】 配列表中の配列番号 2 に示される塩基
   配列を有していることを特徴とする、請求項１に記載の
   一本鎖 DNA 又は該一本鎖 DNA とその相補一本鎖 DNA
   とからなる二本鎖 DNA。
4. 【請求項４】 配列表中の配列番号 1 及び 3 - 15 の
   何れかに示される塩基配列を有していることを特徴とす
   る、請求項２に記載の一本鎖 DNA 又は該一本鎖 DNA と
   その相補一本鎖 DNA とからなる二本鎖 DNA。
5. 【請求項５】 配列表中の配列番号 2 に示されるアミ
   ノ酸配列を有していることを特徴とする、cDNA クロー
   ン。
6. 【請求項６】 配列表中の配列番号 1 及び 3 - 15 の
   何れかに示されるアミノ酸配列を有していることを特徴
   とする、cDNA クローン。
7. 【請求項７】 請求項３に記載の DNA の一部をプロー
   ブとして用いることを特徴とする、ポリメラーゼ連鎖反
   応 (PCR) 法によるヒト C 型肝炎ウイルスの検出並びに
   ヒト C 型肝炎の診断法。
8. 【請求項８】 請求項４に記載の DNA の一部をプロー
   ブとして用いることを特徴とする、ポリメラーゼ連鎖反
   応 (PCR) 法によるヒト C 型肝炎ウイルスの検出並びに
   ヒト C 型肝炎の診断法。
9. 【請求項９】 請求項３に記載の DNA の一部を抗原と
   して用いることを特徴とする、酵素免疫法 (EIA)、血球
   凝集法又はラテックス凝集法による抗ヒト C型肝炎ウイ
   ルス抗体の検出並びにヒト C 型肝炎の診断法。
10. 【請求項１０】 請求項４に記載の DNA の一部を抗原
    として用いることを特徴とする、酵素免疫法 (EIA)、血
    球凝集法又はラテックス凝集法による抗ヒトC 型肝炎ウ
    イルス抗体の検出並びにヒト C 型肝炎の診断法。